{"id":"https://openalex.org/W2105039552","doi":"https://doi.org/10.1104/pp.006353","title":"hpRNA-Mediated Targeting of the Arabidopsis FAD2 Gene Gives Highly Efficient and Stable Silencing","display_name":"hpRNA-Mediated Targeting of the Arabidopsis FAD2 Gene Gives Highly Efficient and Stable Silencing","publication_year":2002,"publication_date":"2002-08-01","ids":{"openalex":"https://openalex.org/W2105039552","doi":"https://doi.org/10.1104/pp.006353","mag":"2105039552","pmid":"https://pubmed.ncbi.nlm.nih.gov/12177485","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/166760"},"language":"en","primary_location":{"id":"doi:10.1104/pp.006353","doi":"https://doi.org/10.1104/pp.006353","is_oa":false,"landing_page_url":"https://doi.org/10.1104/pp.006353","pdf_url":null,"source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC166760/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081665558","display_name":"Peter A. Stoutjesdijk","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1292875679","display_name":"Commonwealth Scientific and Industrial Research Organisation","ror":"https://ror.org/03qn8fb07","country_code":"AU","type":"government","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4387156119"]},{"id":"https://openalex.org/I4210152679","display_name":"Plant Industry","ror":"https://ror.org/05jg9pj51","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4210152679","https://openalex.org/I4387156119"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Peter A. Stoutjesdijk","raw_affiliation_strings":["Commonwealth Scientific and Industrial Research Organization Plant Industry, P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Commonwealth Scientific and Industrial Research Organization Plant Industry, P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia","institution_ids":["https://openalex.org/I1292875679","https://openalex.org/I4210152679"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055102597","display_name":"Surinder Pal Singh","orcid":"https://orcid.org/0000-0003-4488-8147","observed_orcids":["https://orcid.org/0000-0003-4488-8147"]},"institutions":[{"id":"https://openalex.org/I1292875679","display_name":"Commonwealth Scientific and Industrial Research Organisation","ror":"https://ror.org/03qn8fb07","country_code":"AU","type":"government","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4387156119"]},{"id":"https://openalex.org/I4210152679","display_name":"Plant Industry","ror":"https://ror.org/05jg9pj51","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4210152679","https://openalex.org/I4387156119"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Surinder P. Singh","raw_affiliation_strings":["Commonwealth Scientific and Industrial Research Organization Plant Industry, P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Commonwealth Scientific and Industrial Research Organization Plant Industry, P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia","institution_ids":["https://openalex.org/I1292875679","https://openalex.org/I4210152679"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100345161","display_name":"Qing Liu","orcid":"https://orcid.org/0000-0001-6617-6859","observed_orcids":["https://orcid.org/0000-0001-6617-6859"]},"institutions":[{"id":"https://openalex.org/I1292875679","display_name":"Commonwealth Scientific and Industrial Research Organisation","ror":"https://ror.org/03qn8fb07","country_code":"AU","type":"government","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4387156119"]},{"id":"https://openalex.org/I4210152679","display_name":"Plant Industry","ror":"https://ror.org/05jg9pj51","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4210152679","https://openalex.org/I4387156119"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Qing Liu","raw_affiliation_strings":["Commonwealth Scientific and Industrial Research Organization Plant Industry, P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Commonwealth Scientific and Industrial Research Organization Plant Industry, P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia","institution_ids":["https://openalex.org/I1292875679","https://openalex.org/I4210152679"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090022375","display_name":"Clive Hurlstone","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1292875679","display_name":"Commonwealth Scientific and Industrial Research Organisation","ror":"https://ror.org/03qn8fb07","country_code":"AU","type":"government","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4387156119"]},{"id":"https://openalex.org/I4210152679","display_name":"Plant Industry","ror":"https://ror.org/05jg9pj51","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4210152679","https://openalex.org/I4387156119"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Clive J. Hurlstone","raw_affiliation_strings":["Commonwealth Scientific and Industrial Research Organization Plant Industry, P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Commonwealth Scientific and Industrial Research Organization Plant Industry, P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia","institution_ids":["https://openalex.org/I1292875679","https://openalex.org/I4210152679"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075047285","display_name":"Peter M. Waterhouse","orcid":"https://orcid.org/0000-0002-2812-3753","observed_orcids":["https://orcid.org/0000-0002-2812-3753"]},"institutions":[{"id":"https://openalex.org/I1292875679","display_name":"Commonwealth Scientific and Industrial Research Organisation","ror":"https://ror.org/03qn8fb07","country_code":"AU","type":"government","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4387156119"]},{"id":"https://openalex.org/I4210152679","display_name":"Plant Industry","ror":"https://ror.org/05jg9pj51","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4210152679","https://openalex.org/I4387156119"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Peter A. Waterhouse","raw_affiliation_strings":["Commonwealth Scientific and Industrial Research Organization Plant Industry, P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Commonwealth Scientific and Industrial Research Organization Plant Industry, P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia","institution_ids":["https://openalex.org/I1292875679","https://openalex.org/I4210152679"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5041635820","display_name":"Allan G. Green","orcid":"https://orcid.org/0000-0002-3248-2089","observed_orcids":["https://orcid.org/0000-0002-3248-2089"]},"institutions":[{"id":"https://openalex.org/I1292875679","display_name":"Commonwealth Scientific and Industrial Research Organisation","ror":"https://ror.org/03qn8fb07","country_code":"AU","type":"government","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4387156119"]},{"id":"https://openalex.org/I4210152679","display_name":"Plant Industry","ror":"https://ror.org/05jg9pj51","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4210152679","https://openalex.org/I4387156119"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Allan G. Green","raw_affiliation_strings":["Commonwealth Scientific and Industrial Research Organization Plant Industry, P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Commonwealth Scientific and Industrial Research Organization Plant Industry, P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia","institution_ids":["https://openalex.org/I1292875679","https://openalex.org/I4210152679"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3851,"currency":"USD","value_usd":3851},"apc_paid":null,"fwci":15.4555,"has_fulltext":false,"cited_by_count":175,"citation_normalized_percentile":{"value":0.99070312,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"129","issue":"4","first_page":"1723","last_page":"1731"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.6176999807357788,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.6176999807357788,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.058800000697374344,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.04780000075697899,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fad2","display_name":"FAD2","score":0.8299999833106995},{"id":"https://openalex.org/keywords/delta12-desaturase","display_name":"\u039412 desaturase","score":0.8479999899864197},{"id":"https://openalex.org/keywords/post-transcriptional-gene-silencing","display_name":"post-transcriptional gene silencing","score":0.8100000023841858},{"id":"https://openalex.org/keywords/co-suppression","display_name":"co-suppression","score":0.8320000171661377},{"id":"https://openalex.org/keywords/hairpin-rna","display_name":"hairpin RNA","score":0.9369999766349792},{"id":"https://openalex.org/keywords/3-untranslated-region","display_name":"3' untranslated region","score":0.8270000219345093},{"id":"https://openalex.org/keywords/seed-quality","display_name":"seed quality","score":0.5230000019073486},{"id":"https://openalex.org/keywords/arabidopsis-thaliana","display_name":"Arabidopsis thaliana","score":0.8019999861717224}],"concepts":[{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.8851626515388489},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.6091986298561096},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5709388256072998},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5640337467193604},{"id":"https://openalex.org/C2779491563","wikidata":"https://www.wikidata.org/wiki/Q157892","display_name":"Arabidopsis","level":4,"score":0.5184369087219238},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5163113474845886},{"id":"https://openalex.org/C91779695","wikidata":"https://www.wikidata.org/wiki/Q3780824","display_name":"Coding region","level":3,"score":0.49462029337882996},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4940688908100128},{"id":"https://openalex.org/C190232843","wikidata":"https://www.wikidata.org/wiki/Q1950808","display_name":"Small hairpin RNA","level":4,"score":0.4908023774623871},{"id":"https://openalex.org/C84597430","wikidata":"https://www.wikidata.org/wiki/Q106227","display_name":"Locus (genetics)","level":3,"score":0.4658658504486084},{"id":"https://openalex.org/C2191507","wikidata":"https://www.wikidata.org/wiki/Q7277185","display_name":"RNA silencing","level":5,"score":0.45567190647125244},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.35566431283950806},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.332773357629776}],"mesh":[{"descriptor_ui":"D005816","descriptor_name":"Genetic Complementation Test","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012639","descriptor_name":"Seeds","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012639","descriptor_name":"Seeds","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013489","descriptor_name":"Suppression, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016337","descriptor_name":"RNA, Catalytic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016337","descriptor_name":"RNA, Catalytic","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018749","descriptor_name":"RNA, Plant","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018749","descriptor_name":"RNA, Plant","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020868","descriptor_name":"Gene Silencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D034743","descriptor_name":"RNA-Induced Silencing Complex","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D034743","descriptor_name":"RNA-Induced Silencing Complex","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D044943","descriptor_name":"Fatty Acid Desaturases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D044943","descriptor_name":"Fatty Acid Desaturases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1104/pp.006353","doi":"https://doi.org/10.1104/pp.006353","is_oa":false,"landing_page_url":"https://doi.org/10.1104/pp.006353","pdf_url":null,"source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},{"id":"pmid:12177485","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12177485","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant physiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:166760","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC166760/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:166760","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC166760/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiol","raw_type":"Text"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","score":0.6735000014305115,"display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W302428862","https://openalex.org/W1497535479","https://openalex.org/W1509789978","https://openalex.org/W1513717105","https://openalex.org/W1562354168","https://openalex.org/W1565824301","https://openalex.org/W1583821488","https://openalex.org/W1647075334","https://openalex.org/W1856117416","https://openalex.org/W1932753289","https://openalex.org/W1972937465","https://openalex.org/W1975822917","https://openalex.org/W1976857400","https://openalex.org/W2003520310","https://openalex.org/W2005839167","https://openalex.org/W2008162435","https://openalex.org/W2008417030","https://openalex.org/W2015456365","https://openalex.org/W2024556021","https://openalex.org/W2026120652","https://openalex.org/W2034285366","https://openalex.org/W2035282111","https://openalex.org/W2054371299","https://openalex.org/W2070428634","https://openalex.org/W2075362515","https://openalex.org/W2077489356","https://openalex.org/W2089271124","https://openalex.org/W2106629711","https://openalex.org/W2118472374","https://openalex.org/W2144634347","https://openalex.org/W2150816271","https://openalex.org/W2155985666","https://openalex.org/W2159190071","https://openalex.org/W2165558713","https://openalex.org/W2174731688","https://openalex.org/W2335912748","https://openalex.org/W2597399759","https://openalex.org/W6676231913","https://openalex.org/W7033783247"],"related_works":["https://openalex.org/W2226823373","https://openalex.org/W2270274298","https://openalex.org/W2155295182","https://openalex.org/W829723946","https://openalex.org/W1974411647","https://openalex.org/W2356854071","https://openalex.org/W1565824301","https://openalex.org/W2370168679","https://openalex.org/W2995869075","https://openalex.org/W2009400937"],"abstract_inverted_index":{"The":[0,25],"endogenous":[1,270],"Delta":[2,47,112],"12-desaturase":[3,48,113],"gene":[4,209,272],"(FAD2)":[5],"in":[6,58,71,90,183,221,303],"Arabidopsis":[7],"was":[8,50,79,149,158,177,191],"targeted":[9,267],"for":[10,81,162,257,293],"silencing":[11,132,148,210],"using":[12],"seed-specific":[13],"cosuppression":[14],"(CS),":[15],"hairpin":[16],"(HP)":[17],"RNA":[18,217],"(hpRNA),":[19],"and":[20,63,84,233,299],"intron-spliced":[21],"HP":[22,102],"(iHP)":[23],"constructs.":[24,165],"iHP":[26,45,77,265],"construct,":[27,103,124],"incorporating":[28],"the":[29,34,38,59,101,105,122,126,145,152,163,184,192,194,205,235,246,258],"120-bp":[30],"3'-untranslated":[31],"region":[32,129],"of":[33,41,93,97,118,143,147,169,179,223,239,248,296],"FAD2":[35,72,106],"gene,":[36],"gave":[37,109],"highest":[39],"degree":[40,92,146],"silencing.":[42,94],"In":[43,139],"some":[44],"lines":[46],"activity":[49],"reduced":[51,111],"to":[52,261,276],"levels":[53],"as":[54,56],"low":[55],"those":[57],"null":[60],"fad2-1":[61],"mutant,":[62],"every":[64],"primary":[65],"transformant":[66],"showed":[67,85,131],"a":[68,134,173,197,230,290],"pronounced":[69,161],"reduction":[70],"activity.":[73],"One":[74],"highly":[75],"silenced":[76,242],"line":[78,188],"propagated":[80],"five":[82],"generations":[83],"no":[86],"reversion":[87],"or":[88,215],"diminution":[89],"its":[91],"About":[95],"75%":[96],"plants":[98,119,243],"transformed":[99,120],"with":[100,121,204],"targeting":[104],"coding":[107,128],"region,":[108],"dramatically":[110],"activity,":[114],"whereas":[115],"approximately":[116],"50%":[117],"CS":[123,164,187,224],"containing":[125],"same":[127],"sequence,":[130],"at":[133,229],"much":[135,159],"less":[136],"profound":[137],"level.":[138],"all":[140],"three":[141,167],"types":[142,168],"constructs,":[144],"increased":[150],"when":[151],"transgenes":[153],"were":[154],"homozygous,":[155],"but":[156,182],"this":[157,190,288],"more":[160],"All":[166],"construct":[170],"could":[171],"give":[172],"single":[174,231],"locus":[175,195,232],"that":[176,207,218,234,250,264,280],"capable":[178],"effective":[180,237],"silencing,":[181],"one":[185],"such":[186],"where":[189],"case,":[193],"had":[196],"complex":[198,226],"insertion":[199,227],"pattern.":[200],"This":[201],"is":[202,211,219,244],"consistent":[203],"concept":[206],"posttranscriptional":[208],"induced":[212],"by":[213,225,245],"double-stranded,":[214],"self-complementary,":[216],"formed":[220],"cases":[222],"patterns":[228],"most":[236],"way":[238],"generating":[240],"profoundly":[241],"use":[247],"constructs":[249,266],"encode":[251],"hpRNAs.":[252],"Furthermore,":[253],"these":[254],"results":[255],"demonstrate":[256],"first":[259],"time,":[260],"our":[262],"knowledge,":[263],"against":[268],"an":[269],"seed-expressed":[271],"are":[273,281],"clearly":[274],"able":[275],"generate":[277],"phenotypic":[278],"changes":[279],"inherited":[282],"stably":[283],"over":[284],"several":[285],"generations,":[286],"making":[287],"approach":[289],"reliable":[291],"technique":[292],"genetic":[294],"modification":[295],"seed":[297],"quality":[298],"possibly":[300],"other":[301],"traits":[302],"agricultural":[304],"plants.":[305]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":11},{"year":2015,"cited_by_count":9},{"year":2014,"cited_by_count":11},{"year":2013,"cited_by_count":9},{"year":2012,"cited_by_count":10}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}
