{"id":"https://openalex.org/W2104975701","doi":"https://doi.org/10.1152/ajpcell.00563.2004","title":"Identification and characterization of novel IGFBP5 interacting protein: evidence IGFBP5-IP is a potential regulator of osteoblast cell proliferation","display_name":"Identification and characterization of novel IGFBP5 interacting protein: evidence IGFBP5-IP is a potential regulator of osteoblast cell proliferation","publication_year":2005,"publication_date":"2005-11-03","ids":{"openalex":"https://openalex.org/W2104975701","doi":"https://doi.org/10.1152/ajpcell.00563.2004","mag":"2104975701","pmid":"https://pubmed.ncbi.nlm.nih.gov/16269403","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2902994"},"language":"en","primary_location":{"id":"doi:10.1152/ajpcell.00563.2004","doi":"https://doi.org/10.1152/ajpcell.00563.2004","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajpcell.00563.2004","pdf_url":null,"source":{"id":"https://openalex.org/S62704344","display_name":"American Journal of Physiology-Cell Physiology","issn_l":"0363-6143","issn":["0363-6143","1522-1563"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320261","host_organization_name":"American Physical Society","host_organization_lineage":["https://openalex.org/P4310320261"],"host_organization_lineage_names":["American Physical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Cell Physiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2902994","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044913956","display_name":"Yousef G. Amaar","orcid":"https://orcid.org/0000-0002-5525-3422","observed_orcids":["https://orcid.org/0000-0002-5525-3422"]},"institutions":[{"id":"https://openalex.org/I4210116750","display_name":"Jerry L. Pettis Memorial VA Medical Center","ror":"https://ror.org/02bqrry13","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1322918889","https://openalex.org/I2799886695","https://openalex.org/I4210116750","https://openalex.org/I4210138663","https://openalex.org/I4210141570"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yousef G. Amaar","raw_affiliation_strings":["Musculoskeletal Disease Center, PO Box 151, Jerry L. Pettis Veterans Affairs Medical Center, 11201 Benton St., Loma Linda, CA 92357, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Musculoskeletal Disease Center, PO Box 151, Jerry L. Pettis Veterans Affairs Medical Center, 11201 Benton St., Loma Linda, CA 92357, USA","institution_ids":["https://openalex.org/I4210116750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062086691","display_name":"Blanca Tapia","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Blanca Tapia","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029231318","display_name":"Shin\u2010Tai Chen","orcid":"https://orcid.org/0000-0003-1571-2687","observed_orcids":["https://orcid.org/0000-0003-1571-2687"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Shin-Tai Chen","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024383216","display_name":"David J. Baylink","orcid":"https://orcid.org/0000-0001-8937-5623","observed_orcids":["https://orcid.org/0000-0001-8937-5623"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"David J. Baylink","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5054497725","display_name":"Subburaman Mohan","orcid":"https://orcid.org/0000-0003-0063-986X","observed_orcids":["https://orcid.org/0000-0003-0063-986X"]},"institutions":[{"id":"https://openalex.org/I1322918889","display_name":"United States Department of Veterans Affairs","ror":"https://ror.org/05rsv9s98","country_code":"US","type":"government","lineage":["https://openalex.org/I1322918889"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Subburaman Mohan","raw_affiliation_strings":["","UNITED STATES DEPARTMENT OF VETERANS AFFAIRS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]},{"raw_affiliation_string":"UNITED STATES DEPARTMENT OF VETERANS AFFAIRS","institution_ids":["https://openalex.org/I1322918889"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.9878,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.77948937,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":95},"biblio":{"volume":"290","issue":"3","first_page":"C900","last_page":"C906"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10748","display_name":"Growth Hormone and Insulin-like Growth Factors","score":0.5595999956130981,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10748","display_name":"Growth Hormone and Insulin-like Growth Factors","score":0.5595999956130981,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11219","display_name":"Bone Metabolism and Diseases","score":0.41940000653266907,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12110","display_name":"Bone and Dental Protein Studies","score":0.004399999976158142,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/igfbp-5","display_name":"IGFBP-5","score":0.9380000233650208},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.8500000238418579},{"id":"https://openalex.org/keywords/osteoblast-proliferation","display_name":"osteoblast proliferation","score":0.972000002861023},{"id":"https://openalex.org/keywords/osteosarcoma","display_name":"osteosarcoma","score":0.8090000152587891},{"id":"https://openalex.org/keywords/bone-formation","display_name":"bone formation","score":0.6330000162124634},{"id":"https://openalex.org/keywords/sirna-knockdown","display_name":"siRNA knockdown","score":0.7310000061988831}],"concepts":[{"id":"https://openalex.org/C71829478","wikidata":"https://www.wikidata.org/wiki/Q901305","display_name":"Immunoprecipitation","level":3,"score":0.546265184879303},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.5292313098907471},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5289345383644104},{"id":"https://openalex.org/C2778260815","wikidata":"https://www.wikidata.org/wiki/Q917177","display_name":"Osteoblast","level":3,"score":0.5077563524246216},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.49175146222114563},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3895508646965027},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.38340094685554504},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.35191571712493896},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2844706177711487},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.160727858543396}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010006","descriptor_name":"Osteoblasts","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D010006","descriptor_name":"Osteoblasts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020868","descriptor_name":"Gene Silencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D049109","descriptor_name":"Cell Proliferation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1152/ajpcell.00563.2004","doi":"https://doi.org/10.1152/ajpcell.00563.2004","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajpcell.00563.2004","pdf_url":null,"source":{"id":"https://openalex.org/S62704344","display_name":"American Journal of Physiology-Cell Physiology","issn_l":"0363-6143","issn":["0363-6143","1522-1563"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320261","host_organization_name":"American Physical Society","host_organization_lineage":["https://openalex.org/P4310320261"],"host_organization_lineage_names":["American Physical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Cell Physiology","raw_type":"journal-article"},{"id":"pmid:16269403","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16269403","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American journal of physiology. Cell physiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2902994","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2902994","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Am J Physiol Cell Physiol","raw_type":"Text"},{"id":"mag:2469817306","doi":null,"is_oa":false,"landing_page_url":"https://ajpcell.physiology.org/content/ajpcell/290/3/c900.full.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2902994","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2902994","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Am J Physiol Cell Physiol","raw_type":"Text"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2494010510","display_name":"Endosteal Bone Volume Regulation and Osteoporosis","funder_award_id":"R01AR031062","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"},{"id":"https://openalex.org/G500157944","display_name":null,"funder_award_id":"AR 31062","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"}],"funders":[{"id":"https://openalex.org/F4320306127","display_name":"U.S. Department of Veterans Affairs","ror":"https://ror.org/05rsv9s98"},{"id":"https://openalex.org/F4320315885","display_name":"Australian Government","ror":"https://ror.org/0314h5y94"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337362","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W112329086","https://openalex.org/W658743016","https://openalex.org/W1611419331","https://openalex.org/W1966905303","https://openalex.org/W1977173084","https://openalex.org/W1977186059","https://openalex.org/W1977236952","https://openalex.org/W1997806989","https://openalex.org/W2006345660","https://openalex.org/W2013340095","https://openalex.org/W2014264595","https://openalex.org/W2015843482","https://openalex.org/W2017887446","https://openalex.org/W2020368904","https://openalex.org/W2027454234","https://openalex.org/W2036108278","https://openalex.org/W2041228153","https://openalex.org/W2044856415","https://openalex.org/W2053296976","https://openalex.org/W2056983956","https://openalex.org/W2064731204","https://openalex.org/W2072543946","https://openalex.org/W2077689651","https://openalex.org/W2077956417","https://openalex.org/W2080958662","https://openalex.org/W2082312247","https://openalex.org/W2085051326","https://openalex.org/W2086867296","https://openalex.org/W2087475258","https://openalex.org/W2098100188","https://openalex.org/W2140491527","https://openalex.org/W2144634347","https://openalex.org/W2278625953"],"related_works":["https://openalex.org/W2469817306","https://openalex.org/W2090277249","https://openalex.org/W2085051326","https://openalex.org/W2021306369","https://openalex.org/W2127013923","https://openalex.org/W7098751986","https://openalex.org/W1569741533","https://openalex.org/W1979725275","https://openalex.org/W1970699667","https://openalex.org/W2086867296"],"abstract_inverted_index":{"Insulin-like":[0],"growth":[1,24,40,241],"factor":[2,25,41],"binding":[3,17,46],"protein-5":[4],"(IGFBP5)":[5],"is":[6,135,166],"a":[7,15,23,50,86,113,121,208,215],"multifunctional":[8],"protein,":[9,18],"which":[10,179],"acts":[11],"not":[12],"only":[13],"as":[14,22,83,237],"traditional":[16],"but":[19],"also":[20],"functions":[21],"independent":[26],"of":[27,43,47,61,66,90,99,189,195,199,228,240,244],"IGFs":[28],"to":[29,49,53,63,79,112,182],"stimulate":[30],"bone":[31,252],"formation.":[32],"It":[33],"has":[34],"been":[35],"predicted":[36],"that":[37,77,102,123,142,163,206,233],"the":[38,91,100,106,164,187,193,197,226],"intrinsic":[39],"action":[42],"IGFBP5":[44,48,62,80,82,115,134,146,245],"involves":[45],"putative":[51],"receptor":[52],"induce":[54],"downstream":[55],"signaling":[56],"pathways":[57],"and/or":[58],"nuclear":[59],"translocation":[60],"influence":[64],"transcription":[65],"genes":[67],"involved":[68],"in":[69,85,125,138,168,175,214,217,251],"osteoblast":[70,202,222],"cell":[71,95,150,177,223],"proliferation/differentiation.":[72],"Our":[73],"study":[74],"indentified":[75],"proteins":[76],"bound":[78],"using":[81,207],"bait":[84,107],"yeast":[87],"two-hybrid":[88],"screen":[89],"U2":[92],"human":[93,172],"osteosarcoma":[94,176],"cDNA":[96],"library.":[97],"One":[98],"clones":[101],"interacted":[103],"strongly":[104],"with":[105],"under":[108],"high-stringency":[109],"conditions":[110],"corresponded":[111],"novel":[114],"interacting":[116],"protein":[117],"(IGFBP5-IP)":[118],"encoded":[119],"by":[120,137],"gene":[122],"resides":[124],"mouse":[126],"chromosome":[127],"10.":[128],"The":[129],"interaction":[130],"between":[131],"IGFBP5-IP":[132,154,200,234],"and":[133,145,149,155,159,174,220,246],"confirmed":[136],"vitro":[139],"coimmunoprecipitation":[140],"studies":[141],"used":[143],"pFlag":[144],"polyclonal":[147],"antibody,":[148],"lysates":[151],"overexpressing":[152],"both":[153,169,218],"IGFBP5.":[156,184],"Northern":[157],"blot":[158],"RT-PCR":[160],"analysis":[161],"showed":[162],"IGFBP-IP":[165],"expressed":[167],"untransformed":[170],"normal":[171],"osteoblasts":[173],"lines,":[178],"are":[180],"known":[181],"produce":[183],"To":[185],"determine":[186],"roles":[188],"IGFBP5-IP,":[190],"we":[191,231],"evaluated":[192],"effect":[194],"blocking":[196],"expression":[198],"on":[201],"proliferation.":[203,224],"We":[204],"found":[205],"IGFBP5-IP-specific":[209],"small":[210],"interfering-hairpin":[211],"plasmid":[212],"resulted":[213],"decrease":[216],"basal":[219],"IGFBP5-induced":[221],"On":[225],"basis":[227],"these":[229],"findings,":[230],"predict":[232],"may":[235],"act":[236],"intracellular":[238],"mediator":[239],"promoting":[242],"actions":[243],"perhaps":[247],"other":[248],"osteoregulatory":[249],"agents":[250],"cells.":[253]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}