{"id":"https://openalex.org/W2104458086","doi":"https://doi.org/10.1083/jcb.153.1.121","title":"Cyclin a Is Destroyed in Prometaphase and Can Delay Chromosome Alignment and Anaphase","display_name":"Cyclin a Is Destroyed in Prometaphase and Can Delay Chromosome Alignment and Anaphase","publication_year":2001,"publication_date":"2001-04-02","ids":{"openalex":"https://openalex.org/W2104458086","doi":"https://doi.org/10.1083/jcb.153.1.121","mag":"2104458086","pmid":"https://pubmed.ncbi.nlm.nih.gov/11285279","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2185531"},"language":"en","primary_location":{"id":"doi:10.1083/jcb.153.1.121","doi":"https://doi.org/10.1083/jcb.153.1.121","is_oa":false,"landing_page_url":"https://doi.org/10.1083/jcb.153.1.121","pdf_url":null,"source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Cell Biology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2185531/pdf/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5018677801","display_name":"Nicole R. den Elzen","orcid":"https://orcid.org/0000-0003-0223-4954","observed_orcids":["https://orcid.org/0000-0003-0223-4954"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nicole den Elzen","raw_affiliation_strings":["aWellcome/Cancer Research Campaign Institute, Cambridge CB2 1QR, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"aWellcome/Cancer Research Campaign Institute, Cambridge CB2 1QR, United Kingdom","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5046956793","display_name":"Jonathon Pines","orcid":"https://orcid.org/0000-0002-5227-6004","observed_orcids":["https://orcid.org/0000-0002-5227-6004"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jonathon Pines","raw_affiliation_strings":["aWellcome/Cancer Research Campaign Institute, Cambridge CB2 1QR, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"aWellcome/Cancer Research Campaign Institute, Cambridge CB2 1QR, United Kingdom","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":5.0003,"has_fulltext":true,"cited_by_count":376,"citation_normalized_percentile":{"value":0.96722028,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":96,"max":100},"biblio":{"volume":"153","issue":"1","first_page":"121","last_page":"136"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10492","display_name":"Microtubule and mitosis dynamics","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10492","display_name":"Microtubule and mitosis dynamics","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10583","display_name":"Cancer-related Molecular Pathways","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cyclin-a","display_name":"cyclin A","score":0.9909999966621399},{"id":"https://openalex.org/keywords/cyclin-degradation","display_name":"cyclin degradation","score":0.6309999823570251},{"id":"https://openalex.org/keywords/prometaphase","display_name":"prometaphase","score":0.8130000233650208},{"id":"https://openalex.org/keywords/chromosome-alignment","display_name":"chromosome alignment","score":0.8999999761581421},{"id":"https://openalex.org/keywords/anaphase","display_name":"anaphase","score":0.7950000166893005},{"id":"https://openalex.org/keywords/sister-chromatid-segregation","display_name":"sister chromatid segregation","score":0.824999988079071},{"id":"https://openalex.org/keywords/26s-proteasome","display_name":"26S proteasome","score":0.9169999957084656},{"id":"https://openalex.org/keywords/cyclin-dependent-kinases","display_name":"cyclin-dependent kinases","score":0.7910000085830688},{"id":"https://openalex.org/keywords/destruction-box","display_name":"destruction box","score":0.7279999852180481},{"id":"https://openalex.org/keywords/spindle-assembly-checkpoint","display_name":"spindle assembly checkpoint","score":0.921999990940094},{"id":"https://openalex.org/keywords/nuclear-envelope-breakdown","display_name":"nuclear envelope breakdown","score":0.8640000224113464},{"id":"https://openalex.org/keywords/mitosis","display_name":"mitosis","score":0.6470000147819519},{"id":"https://openalex.org/keywords/mitotic-cyclins","display_name":"mitotic cyclins","score":0.6970000267028809}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7323907613754272},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.7243909239768982},{"id":"https://openalex.org/C107888121","wikidata":"https://www.wikidata.org/wiki/Q418432","display_name":"Anaphase-promoting complex","level":5,"score":0.6765764951705933},{"id":"https://openalex.org/C9743823","wikidata":"https://www.wikidata.org/wiki/Q5198264","display_name":"Cyclin B","level":5,"score":0.6597678661346436},{"id":"https://openalex.org/C28179978","wikidata":"https://www.wikidata.org/wiki/Q5198263","display_name":"Cyclin A2","level":5,"score":0.659105658531189},{"id":"https://openalex.org/C16438837","wikidata":"https://www.wikidata.org/wiki/Q5198260","display_name":"Cyclin A","level":5,"score":0.6110070943832397},{"id":"https://openalex.org/C175732170","wikidata":"https://www.wikidata.org/wiki/Q9064829","display_name":"Spindle checkpoint","level":5,"score":0.6043483018875122},{"id":"https://openalex.org/C154202817","wikidata":"https://www.wikidata.org/wiki/Q14763006","display_name":"Prometaphase","level":5,"score":0.5745022296905518},{"id":"https://openalex.org/C124320809","wikidata":"https://www.wikidata.org/wiki/Q46292","display_name":"Cyclin-dependent kinase","level":4,"score":0.5639873147010803},{"id":"https://openalex.org/C11882975","wikidata":"https://www.wikidata.org/wiki/Q24781041","display_name":"Cyclin D","level":5,"score":0.5635604858398438},{"id":"https://openalex.org/C54086632","wikidata":"https://www.wikidata.org/wiki/Q5512671","display_name":"G2-M DNA damage checkpoint","level":5,"score":0.48541030287742615},{"id":"https://openalex.org/C53420372","wikidata":"https://www.wikidata.org/wiki/Q22299157","display_name":"Anaphase","level":4,"score":0.4477682411670685},{"id":"https://openalex.org/C6391034","wikidata":"https://www.wikidata.org/wiki/Q6881916","display_name":"Mitotic exit","level":5,"score":0.42648300528526306},{"id":"https://openalex.org/C93304396","wikidata":"https://www.wikidata.org/wiki/Q14763008","display_name":"Mitosis","level":2,"score":0.42082783579826355},{"id":"https://openalex.org/C120089663","wikidata":"https://www.wikidata.org/wiki/Q59250999","display_name":"Cyclin","level":4,"score":0.3985033631324768},{"id":"https://openalex.org/C47432576","wikidata":"https://www.wikidata.org/wiki/Q918594","display_name":"Spindle apparatus","level":4,"score":0.35814064741134644},{"id":"https://openalex.org/C105696609","wikidata":"https://www.wikidata.org/wiki/Q1068809","display_name":"Cell cycle checkpoint","level":4,"score":0.3130822777748108},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.29710853099823},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.22819414734840393},{"id":"https://openalex.org/C85813293","wikidata":"https://www.wikidata.org/wiki/Q188909","display_name":"Cell division","level":3,"score":0.12170478701591492},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.06178003549575806}],"mesh":[{"descriptor_ui":"D000705","descriptor_name":"Anaphase","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D002877","descriptor_name":"Chromosomes, Human","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008677","descriptor_name":"Metaphase","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D008938","descriptor_name":"Mitosis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015415","descriptor_name":"Biomarkers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018844","descriptor_name":"Cyclin-Dependent Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019925","descriptor_name":"Cyclin A","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019925","descriptor_name":"Cyclin A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1083/jcb.153.1.121","doi":"https://doi.org/10.1083/jcb.153.1.121","is_oa":false,"landing_page_url":"https://doi.org/10.1083/jcb.153.1.121","pdf_url":null,"source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Cell Biology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2185531","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2185531/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2185531/pdf/","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Cell Biol","raw_type":"Text"},{"id":"pmh:oai:europepmc.org:917399","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2185531","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:11285279","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11285279","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.285.4436","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.285.4436","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/93/c3/J_Cell_Biol_2001_Apr_2_153(1)_121-136.tar.gz","raw_type":"text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2185531","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2185531/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2185531/pdf/","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Cell Biol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320360","display_name":"University of Bristol","ror":"https://ror.org/0524sp257"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2104458086.pdf","grobid_xml":"https://content.openalex.org/works/W2104458086.grobid-xml"},"referenced_works_count":61,"referenced_works":["https://openalex.org/W93220107","https://openalex.org/W243911327","https://openalex.org/W307602372","https://openalex.org/W373945723","https://openalex.org/W1483030389","https://openalex.org/W1483395792","https://openalex.org/W1517718286","https://openalex.org/W1544564404","https://openalex.org/W1556017388","https://openalex.org/W1646732007","https://openalex.org/W1959249887","https://openalex.org/W1966773208","https://openalex.org/W1978859977","https://openalex.org/W1981772689","https://openalex.org/W1986148855","https://openalex.org/W1991101546","https://openalex.org/W2000921861","https://openalex.org/W2005510835","https://openalex.org/W2005717642","https://openalex.org/W2008290281","https://openalex.org/W2010749631","https://openalex.org/W2011075906","https://openalex.org/W2014887448","https://openalex.org/W2018711267","https://openalex.org/W2022947433","https://openalex.org/W2026730063","https://openalex.org/W2026802787","https://openalex.org/W2029875848","https://openalex.org/W2036856219","https://openalex.org/W2039839254","https://openalex.org/W2042192373","https://openalex.org/W2048735610","https://openalex.org/W2050753155","https://openalex.org/W2064019223","https://openalex.org/W2073501183","https://openalex.org/W2075776549","https://openalex.org/W2075918847","https://openalex.org/W2078239174","https://openalex.org/W2078358000","https://openalex.org/W2084271768","https://openalex.org/W2088006036","https://openalex.org/W2089975983","https://openalex.org/W2096864462","https://openalex.org/W2102653975","https://openalex.org/W2106285650","https://openalex.org/W2106869142","https://openalex.org/W2126683450","https://openalex.org/W2129153399","https://openalex.org/W2129646494","https://openalex.org/W2130483937","https://openalex.org/W2131717330","https://openalex.org/W2137249911","https://openalex.org/W2147220557","https://openalex.org/W2151710109","https://openalex.org/W2154241215","https://openalex.org/W2155212002","https://openalex.org/W2166987747","https://openalex.org/W2324660863","https://openalex.org/W2354650432","https://openalex.org/W2404304740","https://openalex.org/W2411330611"],"related_works":["https://openalex.org/W2029875848","https://openalex.org/W4403061867","https://openalex.org/W2127545681","https://openalex.org/W1483395792","https://openalex.org/W2028834087","https://openalex.org/W2142790777","https://openalex.org/W2098088639","https://openalex.org/W2169971419","https://openalex.org/W2040977036","https://openalex.org/W2954838324"],"abstract_inverted_index":{"Mitosis":[0],"is":[1,30,75,99,134,151,161,170],"controlled":[2],"by":[3,78,137],"the":[4,14,21,37,41,71,79,104,112,128,141,147,155,164,167],"specific":[5],"and":[6,19,40,120,133,175],"timely":[7],"degradation":[8,69,102],"of":[9,84,114,130,144,166],"key":[10],"regulatory":[11],"proteins,":[12],"notably":[13],"mitotic":[15,148],"cyclins":[16],"that":[17,56,110,160],"bind":[18],"activate":[20],"cyclin-dependent":[22],"kinases":[23],"(Cdks).":[24],"In":[25],"animal":[26],"cells,":[27],"cyclin":[28,34,57,93,115,131],"A":[29,58,94,116,132],"always":[31],"degraded":[32,62],"before":[33],"B,":[35],"but":[36,74,96],"exact":[38],"timing":[39],"mechanism":[42],"underlying":[43],"this":[44],"are":[45],"not":[46,76,135,162],"known.":[47],"Here":[48],"we":[49],"use":[50],"live":[51],"cell":[52],"imaging":[53],"to":[54,60,146],"show":[55,109],"begins":[59],"be":[61],"just":[63],"after":[64],"nuclear":[65],"envelope":[66],"breakdown.":[67],"This":[68,124],"requires":[70],"26S":[72],"proteasome,":[73],"affected":[77],"spindle":[80,156,168],"checkpoint.":[81,157],"Neither":[82],"deletion":[83],"its":[85],"destruction":[86],"box":[87],"nor":[88,150],"disrupting":[89],"Cdk":[90,97],"binding":[91,98],"prevents":[92],"proteolysis,":[95],"necessary":[100],"for":[101,172],"at":[103],"correct":[105],"time.":[106],"We":[107],"also":[108],"increasing":[111],"levels":[113],"delays":[117],"chromosome":[118,173],"alignment":[119,174],"sister":[121],"chromatid":[122],"segregation.":[123],"delay":[125],"depends":[126],"on":[127],"proteolysis":[129,159],"caused":[136],"a":[138],"lag":[139],"in":[140],"bipolar":[142],"attachment":[143],"chromosomes":[145],"spindle,":[149],"it":[152],"mediated":[153],"via":[154],"Thus,":[158],"under":[163],"control":[165],"checkpoint":[169],"required":[171],"anaphase.":[176]},"counts_by_year":[{"year":2026,"cited_by_count":9},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":8},{"year":2021,"cited_by_count":16},{"year":2020,"cited_by_count":13},{"year":2019,"cited_by_count":14},{"year":2018,"cited_by_count":10},{"year":2017,"cited_by_count":14},{"year":2016,"cited_by_count":14},{"year":2015,"cited_by_count":17},{"year":2014,"cited_by_count":16},{"year":2013,"cited_by_count":18},{"year":2012,"cited_by_count":12}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}