{"id":"https://openalex.org/W2104155482","doi":null,"title":"Time-Varying Excitation in Fluorescence Spectroscopy for Biological Applications","display_name":"Time-Varying Excitation in Fluorescence Spectroscopy for Biological Applications","publication_year":2007,"publication_date":"2007-01-01","ids":{"openalex":"https://openalex.org/W2104155482","mag":"2104155482"},"language":"en","primary_location":{"id":"pmh:oai:DiVA.org:kth-4399","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4399","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/masterThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4399","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5058173879","display_name":"Gustav Persson","orcid":"https://orcid.org/0000-0002-2995-0275","observed_orcids":["https://orcid.org/0000-0002-2995-0275"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Gustav Persson","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-2995-0275","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5058173879"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9731000065803528,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9731000065803528,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11129","display_name":"Photochemistry and Electron Transfer Studies","score":0.021199999377131462,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11177","display_name":"Spectroscopy and Quantum Chemical Studies","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/time-varying-excitation","display_name":"time-varying excitation","score":0.9700000286102295},{"id":"https://openalex.org/keywords/modulated-excitation","display_name":"modulated excitation","score":0.9900000095367432},{"id":"https://openalex.org/keywords/fluorescence-spectroscopy","display_name":"fluorescence spectroscopy","score":0.8650000095367432},{"id":"https://openalex.org/keywords/biomolecular-dynamics","display_name":"biomolecular dynamics","score":0.8450000286102295},{"id":"https://openalex.org/keywords/single-molecule-fret","display_name":"single-molecule FRET","score":0.9160000085830688},{"id":"https://openalex.org/keywords/retinoid-x-receptor","display_name":"retinoid X receptor","score":0.9850000143051147},{"id":"https://openalex.org/keywords/nuclear-receptors","display_name":"nuclear receptors","score":0.9629999995231628},{"id":"https://openalex.org/keywords/conformational-dynamics","display_name":"conformational dynamics","score":0.9470000267028809},{"id":"https://openalex.org/keywords/rhodamine-6g","display_name":"rhodamine 6G","score":0.9959999918937683},{"id":"https://openalex.org/keywords/fluorescence-correlation-spectroscopy","display_name":"fluorescence correlation spectroscopy","score":0.9409999847412109},{"id":"https://openalex.org/keywords/protonation-kinetics","display_name":"protonation kinetics","score":0.8090000152587891},{"id":"https://openalex.org/keywords/fluorescein","display_name":"fluorescein","score":0.9639999866485596},{"id":"https://openalex.org/keywords/ccd-camera-detection","display_name":"CCD camera detection","score":0.5080000162124634}],"concepts":[{"id":"https://openalex.org/C83581075","wikidata":"https://www.wikidata.org/wiki/Q1361503","display_name":"Excitation","level":2,"score":0.7300645709037781},{"id":"https://openalex.org/C32891209","wikidata":"https://www.wikidata.org/wiki/Q483666","display_name":"Spectroscopy","level":2,"score":0.6884227991104126},{"id":"https://openalex.org/C158711907","wikidata":"https://www.wikidata.org/wiki/Q1768467","display_name":"Fluorescence spectroscopy","level":3,"score":0.6835523843765259},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.6702867150306702},{"id":"https://openalex.org/C133279788","wikidata":"https://www.wikidata.org/wiki/Q7804835","display_name":"Time-resolved spectroscopy","level":3,"score":0.512736439704895},{"id":"https://openalex.org/C192209626","wikidata":"https://www.wikidata.org/wiki/Q190909","display_name":"Focus (optics)","level":2,"score":0.47565075755119324},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.3875848352909088},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3863665461540222},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38554003834724426},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3467661738395691},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.34040170907974243},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.31800076365470886},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.2663227915763855},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.08723419904708862},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:DiVA.org:kth-4399","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4399","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/masterThesis"},{"id":"mag:2104155482","doi":null,"is_oa":false,"landing_page_url":"http://kth.diva-portal.org/smash/get/diva2:12132/fulltext01.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:DiVA.org:kth-4399","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4399","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/masterThesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":62,"referenced_works":["https://openalex.org/W1506004302","https://openalex.org/W1544898168","https://openalex.org/W1611437588","https://openalex.org/W1858860560","https://openalex.org/W1963667377","https://openalex.org/W1969073916","https://openalex.org/W1973948015","https://openalex.org/W1974142829","https://openalex.org/W1976786825","https://openalex.org/W1977420895","https://openalex.org/W1979741894","https://openalex.org/W1981104867","https://openalex.org/W1981195515","https://openalex.org/W1981765336","https://openalex.org/W1982841657","https://openalex.org/W1984748886","https://openalex.org/W1985486964","https://openalex.org/W1986578141","https://openalex.org/W1987741147","https://openalex.org/W1988401928","https://openalex.org/W1995754186","https://openalex.org/W1998001177","https://openalex.org/W1999814206","https://openalex.org/W2012379620","https://openalex.org/W2017934591","https://openalex.org/W2017957530","https://openalex.org/W2024299353","https://openalex.org/W2031562047","https://openalex.org/W2037280577","https://openalex.org/W2039109187","https://openalex.org/W2050636876","https://openalex.org/W2052954631","https://openalex.org/W2061466767","https://openalex.org/W2062515534","https://openalex.org/W2063525641","https://openalex.org/W2063897180","https://openalex.org/W2064757943","https://openalex.org/W2065125587","https://openalex.org/W2067733907","https://openalex.org/W2070061241","https://openalex.org/W2079080809","https://openalex.org/W2081747214","https://openalex.org/W2084873610","https://openalex.org/W2094517427","https://openalex.org/W2097762754","https://openalex.org/W2106607972","https://openalex.org/W2108317418","https://openalex.org/W2117590286","https://openalex.org/W2123838431","https://openalex.org/W2128978059","https://openalex.org/W2133639929","https://openalex.org/W2134867392","https://openalex.org/W2136387231","https://openalex.org/W2137500070","https://openalex.org/W2148825507","https://openalex.org/W2167373328","https://openalex.org/W2167656187","https://openalex.org/W2170969811","https://openalex.org/W2172184136","https://openalex.org/W2316684534","https://openalex.org/W2494402508","https://openalex.org/W3091872384"],"related_works":["https://openalex.org/W2068998399","https://openalex.org/W2121034995","https://openalex.org/W7027452149","https://openalex.org/W2135902258","https://openalex.org/W2165548215","https://openalex.org/W2161712702","https://openalex.org/W1979664466","https://openalex.org/W7095220240","https://openalex.org/W1988989945","https://openalex.org/W3005148783"],"abstract_inverted_index":{"The":[0,230],"focus":[1],"of":[2,12,20,28,54,58,67,75,144,149,162,181,192,202,208,217,237,241,284,297,310,332,335],"this":[3],"thesis":[4],"is":[5,65,79,88,99,196,291],"to":[6,81,113,155,174,189,260,272,299,306,319,330],"explore":[7],"and":[8,46,87,107,130,175,213,226,248],"use":[9,216,296],"the":[10,36,55,59,68,73,160,163,169,184,193,200,205,209,215,238,267,295,301,307,311,321],"benefits":[11],"time-varying":[13],"excitation":[14,30,194,343],"in":[15,39,72,84,159,183,245,278,350,359],"fluorescence":[16,48,186,203,251,268],"spectroscopy":[17,253],"for":[18,52,167,172,287],"studies":[19,353],"biomolecular":[21],"dynamics.":[22],"Two":[23],"new":[24],"techniques":[25],"taking":[26],"advantage":[27],"modulated":[29,276,342],"are":[31,35,50,111],"presented.":[32,292],"Also":[33],"described":[34],"first":[37],"efforts":[38],"a":[40,93,103,279],"project":[41],"where":[42],"single":[43,117],"molecule":[44,118],"FRET":[45],"multi-parameter":[47],"detection":[49],"used":[51,154],"characterization":[53],"conformational":[56],"dynamics":[57],"retinoid":[60],"X":[61],"receptor":[62],"(RXR).":[63],"RXR":[64,134],"one":[66],"most":[69,90,345],"important":[70],"proteins":[71],"group":[74],"nuclear":[76],"receptors.":[77],"It":[78,198,255],"believed":[80],"be":[82,114,153,258],"involved":[83],"many":[85,138,351],"diseases":[86],"hence":[89],"interesting":[91],"as":[92,221],"potential":[94],"drug":[95],"target.":[96],"Our":[97],"study":[98],"at":[100,102,337],"present":[101],"very":[104,348],"early":[105],"stage":[106],"some":[108],"sample":[109],"issues":[110],"still":[112],"resolved.":[115],"However,":[116],"measurements":[119,236,331],"should":[120,256],"give":[121],"insights":[122],"not":[123],"attainable":[124],"by":[125,179,235],"previously":[126],"applied":[127,329],"ensemble":[128],"methods":[129],"help":[131],"explaining":[132],"how":[133],"can":[135],"regulate":[136],"so":[137],"different":[139,190,338],"processes.":[140],"Long-lived":[141],"transient":[142,211,264],"states":[143,178,212,265],"fluorescent":[145],"molecules":[146],"can,":[147],"because":[148],"their":[150],"long":[151],"lifetimes,":[152],"detect":[156],"subtle":[157],"changes":[158,182],"microenvironment":[161],"molecule.":[164],"A":[165,270],"method":[166],"determining":[168],"kinetic":[170,356],"rates":[171],"transitions":[173],"from":[176],"such":[177,220],"registration":[180],"average":[185],"intensity":[187],"related":[188],"modulation":[191,298],"source":[195],"introduced.":[197],"combines":[199],"sensitivity":[201,207],"with":[204,250,275,304,341],"environmental":[206],"long-lived":[210],"allows":[214,282],"slow":[218],"detectors":[219],"CCD":[222],"cameras,":[223],"making":[224],"parallelization":[225],"imaging":[227],"possible":[228],"developments.":[229],"approach":[231],"was":[232,328],"experimentally":[233],"verified":[234],"triplet":[239],"kinetics":[240,334],"rhodamine":[242],"6G":[243],"(Rh6G)":[244],"aqueous":[246],"solution":[247],"compared":[249],"correlation":[252,285,289],"(FCS).":[254],"also":[257],"applicable":[259],"any":[261],"other":[262],"photoinduced":[263],"affecting":[266],"intensity.":[269],"strategy":[271],"combine":[273],"FCS":[274,340],"excitation,":[277],"way":[280],"that":[281],"extraction":[283],"data":[286],"all":[288],"times,":[290],"This":[293],"enables":[294],"optimize":[300],"measurement":[302],"conditions":[303],"respect":[305],"photophysical":[308],"properties":[309],"dyes":[312],"used.":[313],"Measurements":[314],"were":[315],"made":[316],"on":[317],"Rh6G":[318],"verify":[320],"method.":[322],"To":[323],"illustrate":[324],"its":[325],"usefulness,":[326],"it":[327],"protonation":[333],"fluorescein":[336],"pH.":[339],"will":[344],"probably":[346],"prove":[347],"useful":[349],"future":[352],"involving":[354],"multiple":[355],"processes":[357],"occurring":[358],"overlapping":[360],"time":[361],"ranges.":[362]},"counts_by_year":[],"updated_date":"2026-09-04T10:56:28.161419","created_date":"2016-06-24T00:00:00"}