{"id":"https://openalex.org/W2103992736","doi":"https://doi.org/10.1039/b406860b","title":"Rapid PCR in a continuous flow device","display_name":"Rapid PCR in a continuous flow device","publication_year":2004,"publication_date":"2004-01-01","ids":{"openalex":"https://openalex.org/W2103992736","doi":"https://doi.org/10.1039/b406860b","mag":"2103992736","pmid":"https://pubmed.ncbi.nlm.nih.gov/15570378"},"language":"en","primary_location":{"id":"doi:10.1039/b406860b","doi":"https://doi.org/10.1039/b406860b","is_oa":false,"landing_page_url":"https://doi.org/10.1039/b406860b","pdf_url":null,"source":{"id":"https://openalex.org/S116836101","display_name":"Lab on a Chip","issn_l":"1473-0189","issn":["1473-0189","1473-0197"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Lab on a Chip","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5089590532","display_name":"Masahiko Hashimoto","orcid":"https://orcid.org/0000-0002-1355-8738","observed_orcids":["https://orcid.org/0000-0002-1355-8738"]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Masahiko Hashimoto","raw_affiliation_strings":["Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA","institution_ids":["https://openalex.org/I121820613"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109340745","display_name":"Pin-Chuan Chen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Pin-Chuan Chen","raw_affiliation_strings":["Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA","Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA","institution_ids":["https://openalex.org/I121820613"]},{"raw_affiliation_string":"Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA#TAB#","institution_ids":["https://openalex.org/I121820613"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112833806","display_name":"Michael W. Mitchell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael W. Mitchell","raw_affiliation_strings":["Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA","Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA","institution_ids":["https://openalex.org/I121820613"]},{"raw_affiliation_string":"Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA#TAB#","institution_ids":["https://openalex.org/I121820613"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5114009501","display_name":"Dimitris E. Nikitopoulos","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dimitris E. Nikitopoulos","raw_affiliation_strings":["Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA","Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA","institution_ids":["https://openalex.org/I121820613"]},{"raw_affiliation_string":"Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA#TAB#","institution_ids":["https://openalex.org/I121820613"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038208939","display_name":"Steven A. Soper","orcid":"https://orcid.org/0000-0002-8292-7058","observed_orcids":["https://orcid.org/0000-0002-8292-7058"]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Steven A. Soper","raw_affiliation_strings":["Department of Chemistry, Louisiana State University, Baton Rouge, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Louisiana State University, Baton Rouge, USA","institution_ids":["https://openalex.org/I121820613"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5087283200","display_name":"Michael C. Murphy","orcid":"https://orcid.org/0000-0003-1108-3238","observed_orcids":["https://orcid.org/0000-0003-1108-3238"]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael C. Murphy","raw_affiliation_strings":["Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA","Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA","institution_ids":["https://openalex.org/I121820613"]},{"raw_affiliation_string":"Department of Mechanical Engineering, Louisiana State University, Baton Rouge, USA#TAB#","institution_ids":["https://openalex.org/I121820613"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I121820613"],"apc_list":null,"apc_paid":null,"fwci":12.72,"has_fulltext":false,"cited_by_count":218,"citation_normalized_percentile":{"value":0.99376878,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":92,"max":100},"biblio":{"volume":"4","issue":"6","first_page":"638","last_page":"638"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.9473000168800354,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.9473000168800354,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11407","display_name":"Innovative Microfluidic and Catalytic Techniques Innovation","score":0.04830000177025795,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/continuous-flow-pcr","display_name":"continuous-flow PCR","score":0.9330000281333923},{"id":"https://openalex.org/keywords/flow-velocity","display_name":"flow velocity","score":0.7509999871253967},{"id":"https://openalex.org/keywords/temperature-distribution","display_name":"temperature distribution","score":0.7480000257492065},{"id":"https://openalex.org/keywords/thermal-equilibrium","display_name":"thermal equilibrium","score":0.6190000176429749},{"id":"https://openalex.org/keywords/pressure-drop","display_name":"pressure drop","score":0.7820000052452087},{"id":"https://openalex.org/keywords/residence-time","display_name":"residence time","score":0.9710000157356262},{"id":"https://openalex.org/keywords/amplification-efficiency","display_name":"amplification efficiency","score":0.8320000171661377},{"id":"https://openalex.org/keywords/lambda-dna","display_name":"lambda DNA","score":0.6100000143051147}],"concepts":[{"id":"https://openalex.org/C2985264121","wikidata":"https://www.wikidata.org/wiki/Q216320","display_name":"Continuous flow","level":2,"score":0.64295893907547},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.32574066519737244},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3064180016517639},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.2539186477661133},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.20108544826507568}],"mesh":[{"descriptor_ui":"D003198","descriptor_name":"Computer Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003199","descriptor_name":"Computer Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004867","descriptor_name":"Equipment Design","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008956","descriptor_name":"Models, Chemical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010582","descriptor_name":"Bacteriophage lambda","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017022","descriptor_name":"Flow Injection Analysis","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":true},{"descriptor_ui":"D017022","descriptor_name":"Flow Injection Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017076","descriptor_name":"Computer-Aided Design","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019544","descriptor_name":"Equipment Failure Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046210","descriptor_name":"Microfluidic Analytical Techniques","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":true},{"descriptor_ui":"D046210","descriptor_name":"Microfluidic Analytical Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1039/b406860b","doi":"https://doi.org/10.1039/b406860b","is_oa":false,"landing_page_url":"https://doi.org/10.1039/b406860b","pdf_url":null,"source":{"id":"https://openalex.org/S116836101","display_name":"Lab on a Chip","issn_l":"1473-0189","issn":["1473-0189","1473-0197"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Lab on a Chip","raw_type":"journal-article"},{"id":"pmid:15570378","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15570378","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Lab on a chip","raw_type":"Evaluation Study"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.581.9673","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.581.9673","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.gene-quantification.de/hashimoto-lab-on-chip-2004.pdf","raw_type":"text"},{"id":"pmh:oai:repository.lsu.edu:mechanical_engineering_pubs-2835","doi":null,"is_oa":false,"landing_page_url":"https://repository.lsu.edu/mechanical_engineering_pubs/1835","pdf_url":null,"source":{"id":"https://openalex.org/S4210169993","display_name":"Civil War Book Review","issn_l":"1528-6592","issn":["1528-6592"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315936","host_organization_name":"Louisiana State University","host_organization_lineage":["https://openalex.org/P4310315936"],"host_organization_lineage_names":["Louisiana State University"],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Faculty Publications","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4816556358","display_name":"MICRO-INSTRUMENT PLATFORMS FOR GENETIC-BASED ANALYSES","funder_award_id":"R24EB002115","funder_id":"https://openalex.org/F4320337363","funder_display_name":"National Institute of Biomedical Imaging and Bioengineering"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"},{"id":"https://openalex.org/F4320337363","display_name":"National Institute of Biomedical Imaging and Bioengineering","ror":"https://ror.org/00372qc85"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W643748507","https://openalex.org/W1517480469","https://openalex.org/W1966593487","https://openalex.org/W1968541773","https://openalex.org/W1969604868","https://openalex.org/W1974762990","https://openalex.org/W1975763728","https://openalex.org/W1976055891","https://openalex.org/W1976957718","https://openalex.org/W1983099123","https://openalex.org/W1983144058","https://openalex.org/W1993715247","https://openalex.org/W2007621122","https://openalex.org/W2007762328","https://openalex.org/W2014440872","https://openalex.org/W2015258567","https://openalex.org/W2016813397","https://openalex.org/W2023947315","https://openalex.org/W2045086311","https://openalex.org/W2056645539","https://openalex.org/W2060246168","https://openalex.org/W2066644485","https://openalex.org/W2074305200","https://openalex.org/W2077285362","https://openalex.org/W2079635927","https://openalex.org/W2083800486","https://openalex.org/W2094691790","https://openalex.org/W2108638990","https://openalex.org/W2114209260","https://openalex.org/W2130842192","https://openalex.org/W2139814080","https://openalex.org/W2403048238","https://openalex.org/W2594760119","https://openalex.org/W2888782157","https://openalex.org/W4237521406"],"related_works":["https://openalex.org/W2332384601","https://openalex.org/W1995376647","https://openalex.org/W1975228556","https://openalex.org/W2074680190","https://openalex.org/W2087544646","https://openalex.org/W1994182235","https://openalex.org/W2606419063","https://openalex.org/W2024159738","https://openalex.org/W2992315928","https://openalex.org/W2898086287"],"abstract_inverted_index":{"Continuous":[0],"flow":[1,35,45,73,115],"polymerase":[2,301],"chain":[3],"reaction":[4,253],"(CFPCR)":[5],"devices":[6],"are":[7,76],"compact":[8],"reactors":[9],"suitable":[10],"for":[11,44,92,280],"microfabrication":[12],"and":[13,83,110,139,166,192,221,261],"the":[14,25,34,38,42,48,51,93,108,132,137,140,143,161,178,189,193,196,209,262,283,290],"rapid":[15],"amplification":[16,26,99,206,277],"of":[17,41,78,113,142,153,195,208,214,255,282],"target":[18],"DNAs.":[19],"For":[20],"a":[21,84,118,151,181,212,251],"given":[22],"reactor":[23],"design,":[24],"time":[27,87,254,278],"can":[28,67],"be":[29,68,186,248,267],"reduced":[30,85,187],"simply":[31],"by":[32],"increasing":[33],"velocity":[36,216],"through":[37],"isothermal":[39],"zones":[40,200],"device;":[43],"velocities":[46,74,116,231],"near":[47,63],"design":[49],"value,":[50],"PCR":[52],"cocktail":[53],"reaches":[54],"thermal":[55,109],"equilibrium":[56],"at":[57,71,230,294],"each":[58,89,147],"zone":[59,91,149],"quickly,":[60],"so":[61],"that":[62,96],"ideal":[64],"temperature":[65,90,134,141,148,191,197],"profiles":[66],"obtained.":[69],"However,":[70],"high":[72,114],"there":[75],"penalties":[77],"an":[79],"increased":[80,201,203],"pressure":[81],"drop":[82],"residence":[86],"in":[88,117,146,180,250,269],"DNA/reagent":[94,144],"mixture,":[95],"potentially":[97],"affect":[98],"efficiency.":[100],"This":[101],"study":[102],"was":[103,128,159,217,287],"carried":[104],"out":[105],"to":[106,130,188,237,289],"evaluate":[107],"biochemical":[111],"effects":[112],"spiral,":[119],"20":[120,238],"cycle":[121],"CFPCR":[122,210],"device.":[123],"Finite":[124],"element":[125],"analysis":[126],"(FEA)":[127],"used":[129],"determine":[131],"steady-state":[133],"distribution":[135],"along":[136],"micro-channel":[138],"mixture":[145],"as":[150,211],"function":[152,213],"linear":[154,215],"velocity.":[155,204],"The":[156,205,243,275],"critical":[157],"transition":[158,198],"between":[160,199],"denaturation":[162],"(95":[163],"degrees":[164,169,171],"C)":[165,172],"renaturation":[167],"(55":[168],"C-68":[170],"zones;":[173],"above":[174],"6":[175],"mm":[176,235,239],"s(-1)":[177,236,240],"fluid":[179],"passively-cooled":[182],"channel":[183],"could":[184,247,266],"not":[185],"desired":[190],"duration":[194],"with":[202],"performance":[207],"assessed":[218],"using":[219],"500":[220,244],"997":[222,263,284],"base":[223],"pair":[224],"(bp)":[225],"fragments":[226],"from":[227,233],"lambda-DNA.":[228],"Amplifications":[229],"ranging":[232],"1":[234],"were":[241],"investigated.":[242],"bp":[245,264,285],"fragment":[246,265,286],"observed":[249],"total":[252],"1.7":[256],"min":[257,271],"(5.2":[258],"s":[259,273],"cycle(-1))":[260],"detected":[268],"3.2":[270],"(9.7":[272],"cycle(-1)).":[274],"longer":[276],"required":[279],"detection":[281],"due":[288],"device":[291],"being":[292],"operated":[293],"its":[295],"enzyme":[296],"kinetic":[297],"limit":[298],"(i.e.,":[299],"Taq":[300],"deoxynucleotide":[302],"incorporation":[303],"rate).":[304]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":8},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":7},{"year":2014,"cited_by_count":12},{"year":2013,"cited_by_count":17},{"year":2012,"cited_by_count":14}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}