{"id":"https://openalex.org/W210389462","doi":null,"title":"Development of blocking ELISA for detection of antibodies against avian influenza virus of the H7 subtype.","display_name":"Development of blocking ELISA for detection of antibodies against avian influenza virus of the H7 subtype.","publication_year":2008,"publication_date":"2008-10-01","ids":{"openalex":"https://openalex.org/W210389462","mag":"210389462","pmid":"https://pubmed.ncbi.nlm.nih.gov/19122917"},"language":"en","primary_location":{"id":"pmid:19122917","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19122917","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108396013","display_name":"Jui\u2010Hung Shien","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162838928","display_name":"National Chung Hsing University","ror":"https://ror.org/05vn3ca78","country_code":"TW","type":"education","lineage":["https://openalex.org/I162838928"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Jui-Hung Shien","raw_affiliation_strings":["Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan","institution_ids":["https://openalex.org/I162838928"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023862054","display_name":"Lin-Fan Fu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lin-Fan Fu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027625285","display_name":"Jinru Wu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jin-Ru Wu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110064902","display_name":"Min-Chu Cheng","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Min-Chu Cheng","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113689158","display_name":"Happy K. Shieh","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Happy K Shieh","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5113690503","display_name":"Poa\u2010Chun Chang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162838928","display_name":"National Chung Hsing University","ror":"https://ror.org/05vn3ca78","country_code":"TW","type":"education","lineage":["https://openalex.org/I162838928"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Poa-Chun Chang","raw_affiliation_strings":["National Chung Hsing University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Chung Hsing University","institution_ids":["https://openalex.org/I162838928"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.3231,"has_fulltext":false,"cited_by_count":13,"citation_normalized_percentile":{"value":0.78781904,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"41","issue":"5","first_page":"369","last_page":"76"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10167","display_name":"Influenza Virus Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10167","display_name":"Influenza Virus Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11560","display_name":"Animal Disease Management and Epidemiology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/blocking-elisa","display_name":"blocking ELISA","score":0.9559999704360962},{"id":"https://openalex.org/keywords/avian-influenza-virus","display_name":"avian influenza virus","score":0.8500000238418579},{"id":"https://openalex.org/keywords/h7-subtype","display_name":"H7 subtype","score":0.9929999709129333},{"id":"https://openalex.org/keywords/antibody-detection","display_name":"antibody detection","score":0.8960000276565552},{"id":"https://openalex.org/keywords/hemagglutination-inhibition-test","display_name":"hemagglutination inhibition test","score":0.878000020980835},{"id":"https://openalex.org/keywords/recombinant-hemagglutinin","display_name":"recombinant hemagglutinin","score":0.953000009059906},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.9879999756813049},{"id":"https://openalex.org/keywords/serological-diagnosis","display_name":"serological diagnosis","score":0.5059999823570251},{"id":"https://openalex.org/keywords/assay-sensitivity-and-specificity","display_name":"assay sensitivity and specificity","score":0.6439999938011169},{"id":"https://openalex.org/keywords/chickens","display_name":"chickens","score":0.578000009059906}],"concepts":[{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.7907643914222717},{"id":"https://openalex.org/C32611913","wikidata":"https://www.wikidata.org/wiki/Q2166189","display_name":"Titer","level":3,"score":0.7737109661102295},{"id":"https://openalex.org/C2778692840","wikidata":"https://www.wikidata.org/wiki/Q9136673","display_name":"Hemagglutinin (influenza)","level":3,"score":0.6926980018615723},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.6251981258392334},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5670363306999207},{"id":"https://openalex.org/C182072434","wikidata":"https://www.wikidata.org/wiki/Q3985103","display_name":"Hemagglutination assay","level":4,"score":0.5580659508705139},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.5435715317726135},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5386865139007568},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.5330240726470947},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4780200719833374},{"id":"https://openalex.org/C22889606","wikidata":"https://www.wikidata.org/wiki/Q2166189","display_name":"Antibody titer","level":4,"score":0.41482800245285034},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1468987762928009},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.05141264200210571},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000914","descriptor_name":"Antibodies, Viral","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008807","descriptor_name":"Mice, Inbred BALB C","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009980","descriptor_name":"Influenza A virus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009980","descriptor_name":"Influenza A virus","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019267","descriptor_name":"Hemagglutinin Glycoproteins, Influenza Virus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019267","descriptor_name":"Hemagglutinin Glycoproteins, Influenza Virus","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D019267","descriptor_name":"Hemagglutinin Glycoproteins, Influenza Virus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":1,"locations":[{"id":"pmid:19122917","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19122917","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.6240000128746033,"display_name":"Good health and well-being"},{"id":"https://metadata.un.org/sdg/2","score":0.40450000762939453,"display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W177909858","https://openalex.org/W2009296046","https://openalex.org/W2010585539","https://openalex.org/W2023148463","https://openalex.org/W2040707465","https://openalex.org/W2041652700","https://openalex.org/W2088122405","https://openalex.org/W2111323042","https://openalex.org/W2122385379","https://openalex.org/W2126309815","https://openalex.org/W2143835164","https://openalex.org/W2153608890","https://openalex.org/W2170535835","https://openalex.org/W2174064394","https://openalex.org/W2306596008"],"related_works":["https://openalex.org/W2168992617","https://openalex.org/W2101080492","https://openalex.org/W2026692623","https://openalex.org/W3114311763","https://openalex.org/W2992711239","https://openalex.org/W2918809938","https://openalex.org/W2201581527","https://openalex.org/W3113954077","https://openalex.org/W2468299232","https://openalex.org/W2755039611"],"abstract_inverted_index":{"BACKGROUND":[0],"AND":[1],"PURPOSE:":[2],"The":[3,38,63,76,96,125,141,166,199],"conventional":[4],"method":[5],"used":[6],"for":[7,52,207],"subtyping":[8],"of":[9,29,40,54,56,59,98,111,127,151,155,179,209],"antibodies":[10],"against":[11],"avian":[12,57],"influenza":[13,58],"viruses":[14,33],"is":[15,24,65],"hemagglutination":[16],"inhibition":[17],"(HI)":[18],"test.":[19,140],"However,":[20],"the":[21,60,69,81,99,109,112,128,138,148,196,213,217,232],"HI":[22,139,149,163,177,189],"test":[23,160,174,186],"laborious":[25],"and":[26,67,87,108,171,181],"requires":[27],"preparation":[28],"antigen":[30,220],"from":[31,93,120],"viable":[32],"that":[34,216],"might":[35,201],"be":[36,133,222],"hazardous.":[37],"aim":[39],"this":[41],"study":[42],"was":[43,78,101,114,130,143,157],"to":[44,71,132,145,169],"develop":[45],"a":[46,84,88,204],"blocking":[47],"enzyme-linked":[48],"immunosorbent":[49],"assay":[50],"(B-ELISA)":[51],"detection":[53,208],"antibody":[55,86],"H7":[61],"subtype.":[62],"B-ELISA":[64,77,100,113,129,200],"fast":[66],"avoids":[68],"need":[70],"culture":[72],"whole":[73,233],"viruses.":[74],"METHODS:":[75],"based":[79],"on":[80],"reaction":[82],"between":[83],"monoclonal":[85],"recombinant":[89,218],"hemagglutinin":[90,219],"protein":[91],"purified":[92],"Escherichia":[94],"coli.":[95],"specificity":[97,126],"determined":[102,115],"by":[103,116,195],"testing":[104,117],"H7-negative":[105],"field":[106],"sera":[107,118,161,175,187],"sensitivity":[110,142,154,167],"collected":[119],"experimentally":[121],"immunized":[122],"chickens.":[123],"RESULTS:":[124],"found":[131,144],"97.7%":[134],"when":[135,159,173],"compared":[136],"with":[137,147,188,212],"vary":[146],"titer":[150],"sera.":[152],"A":[153],"100%":[156],"achieved":[158],"had":[162,176],"titers":[164,178,190],">or=2(7).":[165],"dropped":[168],"33%":[170],"20%":[172],"2(6)":[180],"2(5),":[182],"respectively.":[183],"Nearly":[184],"all":[185],"<or=2(4)":[191],"were":[192],"scored":[193],"negative":[194],"B-ELISA.":[197],"CONCLUSION:":[198],"serve":[202],"as":[203],"useful":[205],"tool":[206],"H7-specific":[210],"antibodies,":[211],"added":[214],"advantage":[215],"could":[221],"produced":[223],"in":[224,227],"E.":[225],"coli":[226],"large":[228],"quantities,":[229],"without":[230],"handling":[231],"virus.":[234]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}