{"id":"https://openalex.org/W2103291997","doi":"https://doi.org/10.1002/elps.1150190119","title":"Sequencing using capillary electrophoresis of short tandem repeat alleles separated and purified by high performance liquid chromatography","display_name":"Sequencing using capillary electrophoresis of short tandem repeat alleles separated and purified by high performance liquid chromatography","publication_year":1998,"publication_date":"1998-01-01","ids":{"openalex":"https://openalex.org/W2103291997","doi":"https://doi.org/10.1002/elps.1150190119","mag":"2103291997","pmid":"https://pubmed.ncbi.nlm.nih.gov/9511871"},"language":"en","primary_location":{"id":"doi:10.1002/elps.1150190119","doi":"https://doi.org/10.1002/elps.1150190119","is_oa":false,"landing_page_url":"https://doi.org/10.1002/elps.1150190119","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102225304","display_name":"Michael A. Marino","orcid":"https://orcid.org/0000-0003-1290-4229","observed_orcids":["https://orcid.org/0000-0003-1290-4229"]},"institutions":[{"id":"https://openalex.org/I4210151970","display_name":"Armed Forces Institute of Pathology","ror":"https://ror.org/04se3fj97","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210151970"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Michael A. Marino","raw_affiliation_strings":["Center for Medical and Molecular Genetics, Armed Forces Institute of Pathology, Washington, DC, USA. marino@email.afip.osd.mil","Center for Medical and Molecular Genetics, Armed Forces Institue of Pathology, Washington DC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Medical and Molecular Genetics, Armed Forces Institute of Pathology, Washington, DC, USA. marino@email.afip.osd.mil","institution_ids":["https://openalex.org/I4210151970"]},{"raw_affiliation_string":"Center for Medical and Molecular Genetics, Armed Forces Institue of Pathology, Washington DC, USA","institution_ids":["https://openalex.org/I4210151970"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044300649","display_name":"Joseph M. Devaney","orcid":"https://orcid.org/0000-0003-3648-2737","observed_orcids":["https://orcid.org/0000-0003-3648-2737"]},"institutions":[{"id":"https://openalex.org/I181401687","display_name":"American University","ror":"https://ror.org/052w4zt36","country_code":"US","type":"education","lineage":["https://openalex.org/I181401687"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joseph M. Devaney","raw_affiliation_strings":["Department of Chemistry, American University, Washington DC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, American University, Washington DC, USA","institution_ids":["https://openalex.org/I181401687"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064199538","display_name":"Jonathan K. Smith","orcid":"https://orcid.org/0000-0001-8631-7903","observed_orcids":["https://orcid.org/0000-0001-8631-7903"]},"institutions":[{"id":"https://openalex.org/I4210151970","display_name":"Armed Forces Institute of Pathology","ror":"https://ror.org/04se3fj97","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210151970"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jonathan K. Smith","raw_affiliation_strings":["Center for Medical and Molecular Genetics, Armed Forces Institue of Pathology, Washington DC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Medical and Molecular Genetics, Armed Forces Institue of Pathology, Washington DC, USA","institution_ids":["https://openalex.org/I4210151970"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028374192","display_name":"James E. Girard","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I181401687","display_name":"American University","ror":"https://ror.org/052w4zt36","country_code":"US","type":"education","lineage":["https://openalex.org/I181401687"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James E. Girard","raw_affiliation_strings":["Department of Chemistry, American University, Washington DC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, American University, Washington DC, USA","institution_ids":["https://openalex.org/I181401687"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5102225304"],"corresponding_institution_ids":["https://openalex.org/I4210151970"],"apc_list":{"value":3940,"currency":"USD","value_usd":3940},"apc_paid":null,"fwci":3.2871,"has_fulltext":false,"cited_by_count":20,"citation_normalized_percentile":{"value":0.91959775,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"19","issue":"1","first_page":"108","last_page":"118"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.7285000085830688,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.7285000085830688,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10751","display_name":"Forensic and Genetic Research","score":0.15729999542236328,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.05939999967813492,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microsatellite-markers","display_name":"microsatellite markers","score":0.8730000257492065},{"id":"https://openalex.org/keywords/dna-sequencing","display_name":"DNA sequencing","score":0.6069999933242798},{"id":"https://openalex.org/keywords/capillary-electrophoresis","display_name":"capillary electrophoresis","score":0.8939999938011169},{"id":"https://openalex.org/keywords/pcr-product-purification","display_name":"PCR product purification","score":0.8460000157356262},{"id":"https://openalex.org/keywords/heteroduplex-formation","display_name":"heteroduplex formation","score":0.7689999938011169},{"id":"https://openalex.org/keywords/th01","display_name":"TH01","score":0.800000011920929},{"id":"https://openalex.org/keywords/dna-fingerprinting","display_name":"DNA fingerprinting","score":0.671999990940094},{"id":"https://openalex.org/keywords/electrokinetic-injection","display_name":"electrokinetic injection","score":0.8339999914169312},{"id":"https://openalex.org/keywords/sequencing-efficiency","display_name":"sequencing efficiency","score":0.5860000252723694}],"concepts":[{"id":"https://openalex.org/C2900643","wikidata":"https://www.wikidata.org/wiki/Q747468","display_name":"Capillary electrophoresis","level":2,"score":0.7163201570510864},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6622234582901001},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.4995460510253906},{"id":"https://openalex.org/C61320498","wikidata":"https://www.wikidata.org/wiki/Q265193","display_name":"Microsatellite","level":4,"score":0.4623253643512726},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4608321785926819},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4444117248058319},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34300297498703003},{"id":"https://openalex.org/C180754005","wikidata":"https://www.wikidata.org/wiki/Q80726","display_name":"Allele","level":3,"score":0.2918962240219116},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.24517697095870972},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09814867377281189}],"mesh":[{"descriptor_ui":"D000483","descriptor_name":"Alleles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012091","descriptor_name":"Repetitive Sequences, Nucleic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019075","descriptor_name":"Electrophoresis, Capillary","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/elps.1150190119","doi":"https://doi.org/10.1002/elps.1150190119","is_oa":false,"landing_page_url":"https://doi.org/10.1002/elps.1150190119","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},{"id":"pmid:9511871","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9511871","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electrophoresis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W1979532417","https://openalex.org/W1980877103","https://openalex.org/W1985649733","https://openalex.org/W1986501489","https://openalex.org/W2002381459","https://openalex.org/W2016669119","https://openalex.org/W2023313295","https://openalex.org/W2028671182","https://openalex.org/W2040917377","https://openalex.org/W2049494633","https://openalex.org/W2050717506","https://openalex.org/W2053248530","https://openalex.org/W2060884975","https://openalex.org/W2067126730","https://openalex.org/W2071209346","https://openalex.org/W2071550714","https://openalex.org/W2075748014","https://openalex.org/W2077536709","https://openalex.org/W2082765979","https://openalex.org/W2090631526","https://openalex.org/W2093285783","https://openalex.org/W2129370721","https://openalex.org/W2148312059","https://openalex.org/W2411958326"],"related_works":["https://openalex.org/W2000637643","https://openalex.org/W1980469343","https://openalex.org/W1509391050","https://openalex.org/W2029430383","https://openalex.org/W2050321755","https://openalex.org/W2004216642","https://openalex.org/W2049108596","https://openalex.org/W2486571964","https://openalex.org/W3146663272","https://openalex.org/W2090908773"],"abstract_inverted_index":{"Polymerase":[0],"chain":[1],"reaction":[2],"(PCR)":[3],"amplified":[4],"alleles":[5,131,162],"need":[6],"to":[7,17,50,81,112,152,175,183,350],"be":[8,348],"isolated":[9],"and":[10,23,72,106,142,160,197,251,265,294,302,354],"purified":[11],"before":[12],"carrying":[13],"out":[14],"additional":[15,172],"analysis":[16,260],"confirm":[18],"sequence,":[19],"number":[20,303],"of":[21,35,53,79,86,93,130,132,138,150,248,255,279,291,304,315,325],"repeats":[22,306],"microvariants":[24],"within":[25],"a":[26,222,225,239,252,273,326,330,337],"short":[27,134],"tandem":[28,135],"repeat":[29,37,136],"(STR)":[30],"locus.":[31],"Also,":[32],"PCR":[33,87],"amplification":[34],"tetranucleotide":[36],"loci,":[38],"used":[39,182],"in":[40,46,147],"DNA":[41,61,108,115,352],"typing":[42],"assays,":[43],"often":[44],"result":[45],"heteroduplex":[47],"formation,":[48],"adding":[49],"the":[51,77,84,90,113,121,133,185,210,289,297,300,323],"complexity":[52],"analysis.":[54,65],"Sequencing":[55],"reactions":[56],"require":[57],"single":[58,223],"specific":[59],"target":[60],"for":[62,83,218,228,272,307,329],"reliable":[63],"sequencing":[64,116,277,313],"Alkylated":[66],"poly(styrene-divinylbenzene)":[67],"columns":[68],"at":[69],"elevated":[70],"temperature":[71],"gradient":[73],"elution":[74],"conditions":[75],"increase":[76],"efficiency":[78,314],"separation":[80,91,129,293],"allow":[82],"purification":[85,353],"products.":[88],"Using":[89],"technique":[92,124],"ion-pairing":[94],"reverse-phase":[95],"(IPRP)":[96],"high":[97],"performance":[98],"liquid":[99],"chromatography":[100,123],"(HPLC),":[101],"molecular":[102],"biologists":[103],"can":[104,347],"separate":[105],"purify":[107],"fragments":[109],"without":[110],"alteration":[111],"double-stranded":[114],"properties.":[117],"In":[118],"this":[119,320],"study,":[120],"IP-RP":[122],"has":[125],"been":[126],"demonstrated":[127],"by":[128,158,213],"loci":[137],"TH01,":[139],"vWA31,":[140],"F13A01":[141],"FES/":[143],"FPS.":[144],"Alleles":[145],"differing":[146],"size":[148],"range":[149],"12":[151],"4":[153],"base":[154],"pairs":[155],"were":[156,163,207],"separated":[157],"IPRP/HPLC":[159],"individual":[161,333],"peak-captured,":[164],"then":[165],"cycle-sequenced.":[166],"These":[167],"HPLC":[168,292],"fractions":[169],"required":[170],"no":[171],"steps":[173],"prior":[174],"cycle":[176],"sequencing.":[177],"Capillary":[178],"electrophoresis":[179],"(CE)":[180],"was":[181,233,267,282,317],"sequence":[184,301],"alleles.":[186],"Furthermore,":[187],"CE":[188,295],"offers":[189],"advantages":[190],"over":[191],"traditional":[192,203],"slab":[193,341],"methods":[194],"via":[195],"automation":[196],"higher":[198],"applied":[199,349],"voltages.":[200],"Interestingly,":[201],"unlike":[202],"gel":[204,342],"electrophoresis,":[205],"samples":[206],"introduced":[208],"into":[209],"sieving":[211],"matrix":[212],"electrokinetic":[214],"injection,":[215],"which":[216],"allows":[217],"multiple":[219],"injections":[220],"from":[221],"sample,":[224],"key":[226],"feature":[227],"method":[229,321],"development.":[230],"Applied":[231],"voltage":[232],"320":[234],"V":[235],"per":[236],"centimeter":[237],"using":[238,284,340],"nonderivatized":[240],"fused":[241],"silica":[242],"capillary":[243,263],"with":[244],"an":[245],"interior":[246],"diameter":[247],"50":[249],"microm":[250],"total":[253,259],"length":[254],"47":[256],"centimeters.":[257],"The":[258,344],"time":[261],"including":[262],"filling":[264],"pre-electrophoresis":[266],"less":[268],"than":[269],"30":[270],"min":[271],"220-bp":[274],"fragment.":[275],"A":[276],"rate":[278],"530":[280],"bp/h":[281],"achieved":[283],"these":[285],"conditions.":[286],"By":[287],"combining":[288],"techniques":[290,345],"sequencing,":[296],"results":[298],"confirmed":[299],"nucleotide":[305],"each":[308],"STR":[309],"loci.":[310],"An":[311],"average":[312],"97%":[316],"achieved.":[318],"Additionally,":[319],"defined":[322],"absence":[324],"9.3":[327],"microvariant":[328],"TH01":[331],"heterozygous":[332],"previously":[334],"typed":[335],"as":[336],"9,":[338],"9.3/10":[339],"electrophoresis.":[343],"described":[346],"other":[351],"isolation":[355],"problems.":[356]},"counts_by_year":[{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}