{"id":"https://openalex.org/W2103279581","doi":null,"title":"Cloning and expression of human recombinant isoform a of glycine\u2013N\u2013acyltransferase","display_name":"Cloning and expression of human recombinant isoform a of glycine\u2013N\u2013acyltransferase","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W2103279581","mag":"2103279581"},"language":"en","primary_location":{"id":"pmh:oai:repository.nwu.ac.za:10394/9055","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10394/9055","pdf_url":"http://hdl.handle.net/10394/9055","source":{"id":"https://openalex.org/S4306402268","display_name":"Boloka Institutional Repository (North-west University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114027177","host_organization_name":"University of North Carolina at Chapel Hill","host_organization_lineage":["https://openalex.org/I114027177"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10394/9055","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078180567","display_name":"Daniel Andries Grundling","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Daniel Andries Grundling","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5078180567"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.3935999870300293,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.3935999870300293,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.28519999980926514,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.1168999969959259,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.7839999794960022},{"id":"https://openalex.org/keywords/detoxification","display_name":"detoxification","score":0.75},{"id":"https://openalex.org/keywords/amino-acid-conjugation","display_name":"amino acid conjugation","score":0.7760000228881836},{"id":"https://openalex.org/keywords/protein-aggregation","display_name":"protein aggregation","score":0.6330000162124634},{"id":"https://openalex.org/keywords/protein-refolding","display_name":"protein refolding","score":0.7020000219345093},{"id":"https://openalex.org/keywords/baculovirus-expression-system","display_name":"baculovirus expression system","score":0.8230000138282776},{"id":"https://openalex.org/keywords/protein-solubility","display_name":"protein solubility","score":0.6740000247955322},{"id":"https://openalex.org/keywords/inborn-errors-of-metabolism","display_name":"inborn errors of metabolism","score":0.7889999747276306}],"concepts":[{"id":"https://openalex.org/C2780497538","wikidata":"https://www.wikidata.org/wiki/Q1192006","display_name":"Detoxification (alternative medicine)","level":3,"score":0.8059731721878052},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7074267864227295},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5667508840560913},{"id":"https://openalex.org/C27881333","wikidata":"https://www.wikidata.org/wiki/Q864895","display_name":"Biotransformation","level":3,"score":0.518131673336029},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.515463650226593},{"id":"https://openalex.org/C115448650","wikidata":"https://www.wikidata.org/wiki/Q409205","display_name":"Xenobiotic","level":3,"score":0.4266614615917206},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4151197373867035},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.40337634086608887},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2072562575340271},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:repository.nwu.ac.za:10394/9055","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10394/9055","pdf_url":"http://hdl.handle.net/10394/9055","source":{"id":"https://openalex.org/S4306402268","display_name":"Boloka Institutional Repository (North-west University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114027177","host_organization_name":"University of North Carolina at Chapel Hill","host_organization_lineage":["https://openalex.org/I114027177"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2103279581","doi":null,"is_oa":false,"landing_page_url":"https://dspace.nwu.ac.za/bitstream/10394/9055/1/Grundling_DA.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:repository.nwu.ac.za:10394/9055","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10394/9055","pdf_url":"http://hdl.handle.net/10394/9055","source":{"id":"https://openalex.org/S4306402268","display_name":"Boloka Institutional Repository (North-west University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114027177","host_organization_name":"University of North Carolina at Chapel Hill","host_organization_lineage":["https://openalex.org/I114027177"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"sustainable_development_goals":[{"score":0.504800021648407,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320671","display_name":"National Research Foundation","ror":"https://ror.org/05s0g1g46"},{"id":"https://openalex.org/F4320320719","display_name":"Department of Science and Technology, Ministry of Science and Technology, India","ror":"https://ror.org/0101xrq71"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2103279581.pdf","grobid_xml":"https://content.openalex.org/works/W2103279581.grobid-xml"},"referenced_works_count":54,"referenced_works":["https://openalex.org/W588815118","https://openalex.org/W595401227","https://openalex.org/W618249864","https://openalex.org/W1484177930","https://openalex.org/W1511076704","https://openalex.org/W1515879379","https://openalex.org/W1516646376","https://openalex.org/W1526671183","https://openalex.org/W1530639715","https://openalex.org/W1572955611","https://openalex.org/W1859528054","https://openalex.org/W1931534490","https://openalex.org/W1970756887","https://openalex.org/W1985610154","https://openalex.org/W1999851893","https://openalex.org/W2012973022","https://openalex.org/W2019410656","https://openalex.org/W2022658862","https://openalex.org/W2026585629","https://openalex.org/W2028809992","https://openalex.org/W2045298158","https://openalex.org/W2045336546","https://openalex.org/W2051763536","https://openalex.org/W2054149799","https://openalex.org/W2060667909","https://openalex.org/W2068719237","https://openalex.org/W2075470662","https://openalex.org/W2079554796","https://openalex.org/W2079648904","https://openalex.org/W2081067145","https://openalex.org/W2083624318","https://openalex.org/W2088144279","https://openalex.org/W2097100227","https://openalex.org/W2100028870","https://openalex.org/W2111411672","https://openalex.org/W2111760376","https://openalex.org/W2112457344","https://openalex.org/W2113155528","https://openalex.org/W2141426247","https://openalex.org/W2147842931","https://openalex.org/W2154556168","https://openalex.org/W2166645516","https://openalex.org/W2313835802","https://openalex.org/W2418159503","https://openalex.org/W2505184103","https://openalex.org/W2599434612","https://openalex.org/W2799137111","https://openalex.org/W2804007336","https://openalex.org/W2810264404","https://openalex.org/W2893681885","https://openalex.org/W2927471196","https://openalex.org/W3000813216","https://openalex.org/W3102668775","https://openalex.org/W3203867533"],"related_works":["https://openalex.org/W2012275779","https://openalex.org/W7026729704","https://openalex.org/W2147842931","https://openalex.org/W35719212","https://openalex.org/W2560343506","https://openalex.org/W3126640922","https://openalex.org/W1520989189","https://openalex.org/W1983181572","https://openalex.org/W2113155528","https://openalex.org/W7005507542"],"abstract_inverted_index":{"Awareness":[0],"of":[1,12,32,48,51,93,106,115,132,143,188,202,206,272],"detoxification,":[2],"nowadays":[3],"known":[4,37],"as":[5,42,135],"biotransformation,":[6],"has":[7],"become":[8],"an":[9],"integral":[10],"part":[11],"our":[13],"daily":[14],"lives.It":[15],"is":[16,22,41,53,149],"a":[17,43,128,136,155],"modern":[18],"buzz":[19],"word":[20],"that":[21],"used":[23,163,245,252],"to":[24,30,63,90,151,185,246,253,255,264],"promote":[25],"anything":[26],"from":[27],"health":[28],"food":[29],"enhancement":[31],"performance":[33],"in":[34,67,72,97,195,224,260],"sports.Another":[35],"lesser":[36],"application":[38],"for":[39,45,95,140],"detoxification":[40,76,86,142],"therapy":[44],"alleviating":[46],"symptoms":[47],"inborn":[49],"errors":[50],"metabolism.Detoxification":[52],"the":[54,68,107,120,171,181,189,207,257,273],"process":[55],"where":[56],"endogenous":[57],"and":[58,70,83,99,153,192,216,232,240,251,267],"xenobiotic":[59],"metabolites":[60,94],"are":[61,112],"transformed":[62],"less":[64],"harmful":[65,144],"products,":[66],"liver":[69],"kidneys,":[71],"two":[73,113],"phases.Phase":[74],"1":[75],"includes":[77],"oxidation,":[78],"hydroxylation,":[79],"dehydrogenation":[80],"metabolic":[81],"reduction":[82],"hydrolysis.Phase":[84],"2":[85],"uses":[87],"conjugation":[88,110],"reactions":[89],"increase":[91],"hydrophillicty":[92],"excretion":[96],"bile":[98],"urine.Glycine":[100],"N-acyltransferse":[101],"(GLYAT;":[102],"EC":[103],"2.3.1.13)is":[104],"one":[105],"amino":[108],"acid":[109],"enzymes.There":[111],"variants":[114],"human":[116,123],"GLYAT.I":[117],"focused":[118],"on":[119],"full-length":[121],"mRNA":[122],"GLYAT":[124,221,250,275],"isoform":[125],"a,":[126],"with":[127],"long":[129],"term":[130],"view":[131],"using":[133,170,180],"it":[134,148,279],"viable":[137],"therapeutic":[138],"enzyme":[139,234],"enhanced":[141],"metabolites.I":[145],"investigated":[146],"if":[147],"possible":[150],"clone":[152],"express":[154],"biologically":[156,281],"active":[157],"GLYAT.To":[158],"achieve":[159],"this":[160],"goal":[161],"I":[162],"three":[164,226],"expression":[165,169,179,183,194,227],"systems:":[166],"traditional":[167],"bacterial":[168,178],"pET":[172],"system;":[173],"second":[174],"generation":[175],"cold":[176],"shock":[177],"pCOLDTF":[182],"vector":[184],"improve":[186,214,265],"solubility":[187,215,266,271],"recombinant":[190,208,220,249,258,274],"protein;":[191],"baculovirus":[193],"insect":[196],"cells":[197],"since":[198],"therein":[199],"some":[200],"form":[201],"post":[203],"translation":[204],"glycosylation":[205],"protein":[209,259],"can":[210],"occur":[211],"which":[212],"might":[213],"ensure":[217],"biological":[218,269],"activity.The":[219,270],"expressed":[222],"well":[223],"all":[225],"systems":[228],"but":[229,278],"was":[230,243,276],"aggregated":[231,248],"no":[233],"activity":[235],"could":[236],"be":[237],"detected.A":[238],"denature":[239],"renature":[241],"system":[242],"also":[244],"collect":[247],"try":[254],"refold":[256],"appropriate":[261],"refolding":[262],"buffers":[263],"obtain":[268],"improved":[277],"remained":[280],"inactive.":[282]},"counts_by_year":[{"year":2025,"cited_by_count":1}],"updated_date":"2026-09-01T08:31:24.195828","created_date":"2016-06-24T00:00:00"}