{"id":"https://openalex.org/W2103265014","doi":"https://doi.org/10.1016/j.diagmicrobio.2008.05.002","title":"Development and evaluation of a 1-step duplex reverse transcription polymerase chain reaction for differential diagnosis of chikungunya and dengue infection","display_name":"Development and evaluation of a 1-step duplex reverse transcription polymerase chain reaction for differential diagnosis of chikungunya and dengue infection","publication_year":2008,"publication_date":"2008-06-26","ids":{"openalex":"https://openalex.org/W2103265014","doi":"https://doi.org/10.1016/j.diagmicrobio.2008.05.002","mag":"2103265014","pmid":"https://pubmed.ncbi.nlm.nih.gov/18583086"},"language":"en","primary_location":{"id":"doi:10.1016/j.diagmicrobio.2008.05.002","doi":"https://doi.org/10.1016/j.diagmicrobio.2008.05.002","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.diagmicrobio.2008.05.002","pdf_url":null,"source":{"id":"https://openalex.org/S80485324","display_name":"Diagnostic Microbiology and Infectious Disease","issn_l":"0732-8893","issn":["0732-8893","1879-0070"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Diagnostic Microbiology and Infectious Disease","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102757054","display_name":"Paban Kumar Dash","orcid":"https://orcid.org/0000-0003-4322-2044","observed_orcids":["https://orcid.org/0000-0003-4322-2044"]},"institutions":[{"id":"https://openalex.org/I2800900741","display_name":"Defence Research and Development Establishment","ror":"https://ror.org/04sm60f26","country_code":"IN","type":"facility","lineage":["https://openalex.org/I1340206300","https://openalex.org/I2799351866","https://openalex.org/I2800900741","https://openalex.org/I4210150591"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Paban Kumar Dash","raw_affiliation_strings":["Division of Virology, Defence Research and Development Establishment, Gwalior, MP 474 002, India. pabandash@rediffmail.com","Division of Virology, Defence Research and Development Establishment (DRDE), Gwalior, MP 474 002, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Virology, Defence Research and Development Establishment, Gwalior, MP 474 002, India. pabandash@rediffmail.com","institution_ids":["https://openalex.org/I2800900741"]},{"raw_affiliation_string":"Division of Virology, Defence Research and Development Establishment (DRDE), Gwalior, MP 474 002, India","institution_ids":["https://openalex.org/I2800900741"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040776132","display_name":"Manmohan Parida","orcid":"https://orcid.org/0009-0002-6856-2221","observed_orcids":["https://orcid.org/0009-0002-6856-2221"]},"institutions":[{"id":"https://openalex.org/I2800900741","display_name":"Defence Research and Development Establishment","ror":"https://ror.org/04sm60f26","country_code":"IN","type":"facility","lineage":["https://openalex.org/I1340206300","https://openalex.org/I2799351866","https://openalex.org/I2800900741","https://openalex.org/I4210150591"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Manmohan Parida","raw_affiliation_strings":["Division of Virology, Defence Research and Development Establishment (DRDE), Gwalior, MP 474 002, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Virology, Defence Research and Development Establishment (DRDE), Gwalior, MP 474 002, India","institution_ids":["https://openalex.org/I2800900741"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109993473","display_name":"S.R. Santhosh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2800900741","display_name":"Defence Research and Development Establishment","ror":"https://ror.org/04sm60f26","country_code":"IN","type":"facility","lineage":["https://openalex.org/I1340206300","https://openalex.org/I2799351866","https://openalex.org/I2800900741","https://openalex.org/I4210150591"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"S.R. Santhosh","raw_affiliation_strings":["Division of Virology, Defence Research and Development Establishment (DRDE), Gwalior, MP 474 002, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Virology, Defence Research and Development Establishment (DRDE), Gwalior, MP 474 002, India","institution_ids":["https://openalex.org/I2800900741"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001464911","display_name":"Parag Saxena","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2800900741","display_name":"Defence Research and Development Establishment","ror":"https://ror.org/04sm60f26","country_code":"IN","type":"facility","lineage":["https://openalex.org/I1340206300","https://openalex.org/I2799351866","https://openalex.org/I2800900741","https://openalex.org/I4210150591"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Parag Saxena","raw_affiliation_strings":["Division of Virology, Defence Research and Development Establishment (DRDE), Gwalior, MP 474 002, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Virology, Defence Research and Development Establishment (DRDE), Gwalior, MP 474 002, India","institution_ids":["https://openalex.org/I2800900741"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108508250","display_name":"Ambuj Srivastava","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2800900741","display_name":"Defence Research and Development Establishment","ror":"https://ror.org/04sm60f26","country_code":"IN","type":"facility","lineage":["https://openalex.org/I1340206300","https://openalex.org/I2799351866","https://openalex.org/I2800900741","https://openalex.org/I4210150591"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Ambuj Srivastava","raw_affiliation_strings":["Division of Virology, Defence Research and Development Establishment (DRDE), Gwalior, MP 474 002, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Virology, Defence Research and Development Establishment (DRDE), Gwalior, MP 474 002, India","institution_ids":["https://openalex.org/I2800900741"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112376608","display_name":"Mamidi Neeraja","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139669409","display_name":"Nizam's Institute of Medical Sciences","ror":"https://ror.org/01wjz9118","country_code":"IN","type":"education","lineage":["https://openalex.org/I139669409"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Mamidi Neeraja","raw_affiliation_strings":["Department of Microbiology, Nizams Institute of Medical Sciences (NIMS), Hyderabad, AP 500 082, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Nizams Institute of Medical Sciences (NIMS), Hyderabad, AP 500 082, India","institution_ids":["https://openalex.org/I139669409"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113597175","display_name":"V Lakshmi","orcid":"https://orcid.org/0000-0003-2473-5311","observed_orcids":["https://orcid.org/0000-0003-2473-5311"]},"institutions":[{"id":"https://openalex.org/I139669409","display_name":"Nizam's Institute of Medical Sciences","ror":"https://ror.org/01wjz9118","country_code":"IN","type":"education","lineage":["https://openalex.org/I139669409"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"V. Lakshmi","raw_affiliation_strings":["Department of Microbiology, Nizams Institute of Medical Sciences (NIMS), Hyderabad, AP 500 082, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Nizams Institute of Medical Sciences (NIMS), Hyderabad, AP 500 082, India","institution_ids":["https://openalex.org/I139669409"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110257960","display_name":"P. Venkata Rao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2800900741","display_name":"Defence Research and Development Establishment","ror":"https://ror.org/04sm60f26","country_code":"IN","type":"facility","lineage":["https://openalex.org/I1340206300","https://openalex.org/I2799351866","https://openalex.org/I2800900741","https://openalex.org/I4210150591"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"P.V. Lakshmana Rao","raw_affiliation_strings":["Division of Virology, Defence Research and Development Establishment (DRDE), Gwalior, MP 474 002, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Virology, Defence Research and Development Establishment (DRDE), Gwalior, MP 474 002, India","institution_ids":["https://openalex.org/I2800900741"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5102757054"],"corresponding_institution_ids":["https://openalex.org/I2800900741"],"apc_list":{"value":3290,"currency":"USD","value_usd":3290},"apc_paid":null,"fwci":2.288,"has_fulltext":false,"cited_by_count":36,"citation_normalized_percentile":{"value":0.87755577,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"62","issue":"1","first_page":"52","last_page":"57"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12047","display_name":"Viral Infections and Vectors","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11135","display_name":"Virology and Viral Diseases","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/chikungunya","display_name":"chikungunya","score":0.890999972820282},{"id":"https://openalex.org/keywords/dengue","display_name":"dengue","score":0.9789999723434448},{"id":"https://openalex.org/keywords/differential-diagnosis","display_name":"differential diagnosis","score":0.8450000286102295},{"id":"https://openalex.org/keywords/duplex-rt-pcr","display_name":"duplex RT-PCR","score":0.8640000224113464},{"id":"https://openalex.org/keywords/single-tube-assay","display_name":"single-tube assay","score":0.6990000009536743},{"id":"https://openalex.org/keywords/e1-gene","display_name":"E1 gene","score":0.9010000228881836},{"id":"https://openalex.org/keywords/arbovirus-infection","display_name":"arbovirus infection","score":0.7850000262260437},{"id":"https://openalex.org/keywords/coinfection","display_name":"co-infection","score":0.531000018119812},{"id":"https://openalex.org/keywords/viral-genotyping","display_name":"viral genotyping","score":0.7839999794960022},{"id":"https://openalex.org/keywords/phylogenetic-analysis","display_name":"phylogenetic analysis","score":0.7739999890327454},{"id":"https://openalex.org/keywords/rapid-detection","display_name":"rapid detection","score":0.35199999809265137}],"concepts":[{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.8226585388183594},{"id":"https://openalex.org/C2781273456","wikidata":"https://www.wikidata.org/wiki/Q243257","display_name":"Chikungunya","level":3,"score":0.7970868945121765},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6679538488388062},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.6293321847915649},{"id":"https://openalex.org/C2779635636","wikidata":"https://www.wikidata.org/wiki/Q476209","display_name":"Dengue virus","level":3,"score":0.6131451725959778},{"id":"https://openalex.org/C31467283","wikidata":"https://www.wikidata.org/wiki/Q912147","display_name":"Genotyping","level":4,"score":0.5774322748184204},{"id":"https://openalex.org/C533803919","wikidata":"https://www.wikidata.org/wiki/Q30953","display_name":"Dengue fever","level":2,"score":0.5757476091384888},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5701572299003601},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.5278798341751099},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.4882988929748535},{"id":"https://openalex.org/C10389963","wikidata":"https://www.wikidata.org/wiki/Q848328","display_name":"Serotype","level":2,"score":0.4791404902935028},{"id":"https://openalex.org/C4692481","wikidata":"https://www.wikidata.org/wiki/Q169526","display_name":"Reverse transcription polymerase chain reaction","level":4,"score":0.4759252369403839},{"id":"https://openalex.org/C2777474056","wikidata":"https://www.wikidata.org/wiki/Q1111605","display_name":"Alphavirus","level":3,"score":0.4561980366706848},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.43396133184432983},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.31072524189949036},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.16663408279418945},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.08520612120628357}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002646","descriptor_name":"Chikungunya virus","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D002646","descriptor_name":"Chikungunya virus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002646","descriptor_name":"Chikungunya virus","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D003715","descriptor_name":"Dengue","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D003715","descriptor_name":"Dengue","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D003716","descriptor_name":"Dengue Virus","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D003716","descriptor_name":"Dengue Virus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003716","descriptor_name":"Dengue Virus","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D003937","descriptor_name":"Diagnosis, Differential","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007194","descriptor_name":"India","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010802","descriptor_name":"Phylogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014036","descriptor_name":"Togaviridae Infections","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D014036","descriptor_name":"Togaviridae Infections","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.diagmicrobio.2008.05.002","doi":"https://doi.org/10.1016/j.diagmicrobio.2008.05.002","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.diagmicrobio.2008.05.002","pdf_url":null,"source":{"id":"https://openalex.org/S80485324","display_name":"Diagnostic Microbiology and Infectious Disease","issn_l":"0732-8893","issn":["0732-8893","1879-0070"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Diagnostic Microbiology and Infectious Disease","raw_type":"journal-article"},{"id":"pmid:18583086","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18583086","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Diagnostic microbiology and infectious disease","raw_type":"Evaluation Study"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9537000060081482}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W1705600225","https://openalex.org/W1773048998","https://openalex.org/W1971242896","https://openalex.org/W1984621708","https://openalex.org/W1988176421","https://openalex.org/W2002262533","https://openalex.org/W2030514517","https://openalex.org/W2034377840","https://openalex.org/W2043999806","https://openalex.org/W2049347241","https://openalex.org/W2078518982","https://openalex.org/W2080259596","https://openalex.org/W2083524751","https://openalex.org/W2090105219","https://openalex.org/W2092158924","https://openalex.org/W2107922358","https://openalex.org/W2132500342","https://openalex.org/W2133312047","https://openalex.org/W2137415189","https://openalex.org/W2137750232","https://openalex.org/W2146396346","https://openalex.org/W2147763877","https://openalex.org/W2464023451","https://openalex.org/W4320800933"],"related_works":["https://openalex.org/W1963697385","https://openalex.org/W2467721711","https://openalex.org/W2033948036","https://openalex.org/W3096482993","https://openalex.org/W2171508094","https://openalex.org/W1981764106","https://openalex.org/W2037463365","https://openalex.org/W4367681140","https://openalex.org/W1934338847","https://openalex.org/W2282391863"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2016-06-24T00:00:00"}