{"id":"https://openalex.org/W2102989692","doi":"https://doi.org/10.1261/rna.1754109","title":"Absolute quantification of microRNAs by using a universal reference","display_name":"Absolute quantification of microRNAs by using a universal reference","publication_year":2009,"publication_date":"2009-10-27","ids":{"openalex":"https://openalex.org/W2102989692","doi":"https://doi.org/10.1261/rna.1754109","mag":"2102989692","pmid":"https://pubmed.ncbi.nlm.nih.gov/19861428","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2779673"},"language":"en","primary_location":{"id":"doi:10.1261/rna.1754109","doi":"https://doi.org/10.1261/rna.1754109","is_oa":true,"landing_page_url":"https://doi.org/10.1261/rna.1754109","pdf_url":"http://rnajournal.cshlp.org/content/15/12/2375.full.pdf","source":{"id":"https://openalex.org/S187657468","display_name":"RNA","issn_l":"1355-8382","issn":["1355-8382","1469-9001"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RNA","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"http://rnajournal.cshlp.org/content/15/12/2375.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112084237","display_name":"Ute Bissels","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I137469784","display_name":"Miltenyi Biotec (Germany)","ror":"https://ror.org/00qhe6a56","country_code":"DE","type":"company","lineage":["https://openalex.org/I137469784"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ute Bissels","raw_affiliation_strings":["Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany","institution_ids":["https://openalex.org/I137469784"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056855713","display_name":"Stefan Wild","orcid":"https://orcid.org/0000-0003-3227-3049","observed_orcids":["https://orcid.org/0000-0003-3227-3049"]},"institutions":[{"id":"https://openalex.org/I137469784","display_name":"Miltenyi Biotec (Germany)","ror":"https://ror.org/00qhe6a56","country_code":"DE","type":"company","lineage":["https://openalex.org/I137469784"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Stefan Wild","raw_affiliation_strings":["Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany","institution_ids":["https://openalex.org/I137469784"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009160578","display_name":"Stefan Tomiuk","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I137469784","display_name":"Miltenyi Biotec (Germany)","ror":"https://ror.org/00qhe6a56","country_code":"DE","type":"company","lineage":["https://openalex.org/I137469784"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Stefan Tomiuk","raw_affiliation_strings":["Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany","institution_ids":["https://openalex.org/I137469784"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030939184","display_name":"Angela Sarah Holste","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I137469784","display_name":"Miltenyi Biotec (Germany)","ror":"https://ror.org/00qhe6a56","country_code":"DE","type":"company","lineage":["https://openalex.org/I137469784"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Angela Holste","raw_affiliation_strings":["Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany","institution_ids":["https://openalex.org/I137469784"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027878787","display_name":"Markus Hafner","orcid":"https://orcid.org/0000-0002-4336-6518","observed_orcids":["https://orcid.org/0000-0002-4336-6518"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I83399316","display_name":"Rockefeller University","ror":"https://ror.org/0420db125","country_code":"US","type":"education","lineage":["https://openalex.org/I83399316"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Markus Hafner","raw_affiliation_strings":["Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Rockefeller University, New York, New York 10065, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Rockefeller University, New York, New York 10065, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I83399316"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042084987","display_name":"Thomas Tuschl","orcid":"https://orcid.org/0000-0002-6483-5678","observed_orcids":["https://orcid.org/0000-0002-6483-5678"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I83399316","display_name":"Rockefeller University","ror":"https://ror.org/0420db125","country_code":"US","type":"education","lineage":["https://openalex.org/I83399316"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas Tuschl","raw_affiliation_strings":["Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Rockefeller University, New York, New York 10065, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Rockefeller University, New York, New York 10065, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I83399316"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5030992159","display_name":"Andreas Bosio","orcid":"https://orcid.org/0000-0001-9776-2535","observed_orcids":["https://orcid.org/0000-0001-9776-2535"]},"institutions":[{"id":"https://openalex.org/I137469784","display_name":"Miltenyi Biotec (Germany)","ror":"https://ror.org/00qhe6a56","country_code":"DE","type":"company","lineage":["https://openalex.org/I137469784"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Andreas Bosio","raw_affiliation_strings":["Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany","institution_ids":["https://openalex.org/I137469784"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":3.9942,"has_fulltext":true,"cited_by_count":207,"citation_normalized_percentile":{"value":0.9414168,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":97,"max":100},"biblio":{"volume":"15","issue":"12","first_page":"2375","last_page":"2384"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.8916000127792358,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.8916000127792358,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.06759999692440033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.02539999969303608,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mirna-quantification","display_name":"microRNA quantification","score":0.796999990940094},{"id":"https://openalex.org/keywords/absolute-quantification","display_name":"absolute quantification","score":0.9739999771118164},{"id":"https://openalex.org/keywords/mirna-microarray","display_name":"miRNA microarray","score":0.8840000033378601},{"id":"https://openalex.org/keywords/hematopoietic-progenitor-cells","display_name":"hematopoietic progenitor cells","score":0.9290000200271606},{"id":"https://openalex.org/keywords/liver","display_name":"liver","score":0.5709999799728394},{"id":"https://openalex.org/keywords/spike-in-experiments","display_name":"spike-in experiments","score":0.8389999866485596}],"concepts":[{"id":"https://openalex.org/C49305753","wikidata":"https://www.wikidata.org/wiki/Q6826947","display_name":"MiRBase","level":4,"score":0.9450080394744873},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.896342396736145},{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.6725776791572571},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6024994850158691},{"id":"https://openalex.org/C186836561","wikidata":"https://www.wikidata.org/wiki/Q1931516","display_name":"Microarray","level":4,"score":0.5360707640647888},{"id":"https://openalex.org/C95371953","wikidata":"https://www.wikidata.org/wiki/Q591745","display_name":"DNA microarray","level":4,"score":0.501215934753418},{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.4169533848762512},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.35898879170417786},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3289690613746643},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.20208033919334412},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1911177933216095}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D018628","descriptor_name":"Gene Dosage","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020411","descriptor_name":"Oligonucleotide Array Sequence Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D035683","descriptor_name":"MicroRNAs","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1261/rna.1754109","doi":"https://doi.org/10.1261/rna.1754109","is_oa":true,"landing_page_url":"https://doi.org/10.1261/rna.1754109","pdf_url":"http://rnajournal.cshlp.org/content/15/12/2375.full.pdf","source":{"id":"https://openalex.org/S187657468","display_name":"RNA","issn_l":"1355-8382","issn":["1355-8382","1469-9001"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RNA","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2779673","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2779673","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RNA","raw_type":"Text"},{"id":"pmid:19861428","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19861428","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RNA (New York, N.Y.)","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1261/rna.1754109","doi":"https://doi.org/10.1261/rna.1754109","is_oa":true,"landing_page_url":"https://doi.org/10.1261/rna.1754109","pdf_url":"http://rnajournal.cshlp.org/content/15/12/2375.full.pdf","source":{"id":"https://openalex.org/S187657468","display_name":"RNA","issn_l":"1355-8382","issn":["1355-8382","1469-9001"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"RNA","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306082","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2102989692.pdf","grobid_xml":"https://content.openalex.org/works/W2102989692.grobid-xml"},"referenced_works_count":44,"referenced_works":["https://openalex.org/W45534775","https://openalex.org/W1532162953","https://openalex.org/W1976829642","https://openalex.org/W1989373988","https://openalex.org/W2004651171","https://openalex.org/W2008420760","https://openalex.org/W2016987986","https://openalex.org/W2017270642","https://openalex.org/W2022916085","https://openalex.org/W2042206438","https://openalex.org/W2077607729","https://openalex.org/W2097621920","https://openalex.org/W2108314707","https://openalex.org/W2110348656","https://openalex.org/W2117289584","https://openalex.org/W2119251184","https://openalex.org/W2123214754","https://openalex.org/W2126696654","https://openalex.org/W2127299993","https://openalex.org/W2136746865","https://openalex.org/W2141128504","https://openalex.org/W2146046635","https://openalex.org/W2149146453","https://openalex.org/W2150926065","https://openalex.org/W2151868278","https://openalex.org/W2151922790","https://openalex.org/W2155173012","https://openalex.org/W2157795344","https://openalex.org/W2158878989","https://openalex.org/W2161222316","https://openalex.org/W2161426724","https://openalex.org/W2161693225","https://openalex.org/W2162266792","https://openalex.org/W2162674813","https://openalex.org/W2164550342","https://openalex.org/W2166179294","https://openalex.org/W2167100268","https://openalex.org/W2167264963","https://openalex.org/W2167546613","https://openalex.org/W2171833185","https://openalex.org/W2409075727","https://openalex.org/W2748101318","https://openalex.org/W4294107304","https://openalex.org/W7100139990"],"related_works":["https://openalex.org/W1971602763","https://openalex.org/W4200592532","https://openalex.org/W2064709017","https://openalex.org/W2744391314","https://openalex.org/W7095683263","https://openalex.org/W2132064277","https://openalex.org/W2036280113","https://openalex.org/W2411090539","https://openalex.org/W3172548493","https://openalex.org/W2394826813"],"abstract_inverted_index":{"MicroRNAs":[0],"(miRNAs)":[1],"are":[2,136],"a":[3,48,58,99,125,141],"species":[4],"of":[5,52,61,79,107,116,119,128,179],"small":[6],"RNAs":[7],"approximately":[8],"21-23-nucleotides":[9],"long":[10],"that":[11,135],"have":[12,35,84],"been":[13,36,87],"shown":[14],"to":[15,38,163,170],"play":[16],"an":[17,91],"important":[18],"role":[19],"in":[20,47,132,192],"many":[21],"different":[22],"cellular,":[23],"developmental,":[24],"and":[25,40,55,75,104,124,151,195],"physiological":[26],"processes.":[27],"Accordingly,":[28],"numerous":[29],"PCR-,":[30],"sequencing-,":[31],"or":[32,64,173],"hybridization-based":[33],"methods":[34],"established":[37],"identify":[39],"quantify":[41],"miRNAs.":[42,108],"Their":[43],"short":[44],"length":[45],"results":[46],"high":[49],"dynamic":[50],"range":[51],"melting":[53],"temperatures":[54],"therefore":[56],"impedes":[57],"proper":[59],"selection":[60],"detection":[62],"probes":[63,145],"optimized":[65],"PCR":[66],"primers.":[67],"While":[68],"miRNA":[69,158,189],"microarrays":[70],"allow":[71],"for":[72,93,102,146],"massive":[73],"parallel":[74,114],"accurate":[76],"relative":[77],"measurement":[78],"all":[80,147],"known":[81],"miRNAs,":[82],"they":[83],"so":[85],"far":[86],"less":[88],"useful":[89],"as":[90],"assay":[92],"absolute":[94,105],"quantification.":[95],"Here,":[96],"we":[97,187],"present":[98],"microarray-based":[100],"approach":[101],"global":[103],"quantification":[106],"The":[109],"method":[110,181],"relies":[111],"on":[112,140],"the":[113,117,164,177,180],"hybridization":[115],"sample":[118],"interest":[120],"labeled":[121,137],"with":[122,138,161],"Cy5":[123],"universal":[126,165],"reference":[127,166],"954":[129],"synthetic":[130],"miRNAs":[131,153],"equimolar":[133],"concentrations":[134],"Cy3":[139],"microarray":[142],"slide":[143],"containing":[144],"human,":[148],"mouse,":[149],"rat,":[150],"viral":[152],"(miRBase":[154],"12.0).":[155],"Each":[156],"single":[157],"is":[159],"quantified":[160,188],"respect":[162],"canceling":[167],"biases":[168],"related":[169],"sequence,":[171],"labeling,":[172],"hybridization.":[174],"We":[175],"demonstrate":[176],"accuracy":[178],"by":[182],"various":[183],"spike-in":[184],"experiments.":[185],"Furthermore,":[186],"copy":[190],"numbers":[191],"liver":[193],"samples":[194],"CD34(+)/CD133(-)":[196],"hematopoietic":[197],"progenitor":[198],"cells.":[199]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":10},{"year":2024,"cited_by_count":9},{"year":2023,"cited_by_count":11},{"year":2022,"cited_by_count":14},{"year":2021,"cited_by_count":13},{"year":2020,"cited_by_count":9},{"year":2019,"cited_by_count":13},{"year":2018,"cited_by_count":13},{"year":2017,"cited_by_count":13},{"year":2016,"cited_by_count":14},{"year":2015,"cited_by_count":18},{"year":2014,"cited_by_count":20},{"year":2013,"cited_by_count":9},{"year":2012,"cited_by_count":12}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}
