{"id":"https://openalex.org/W2102825615","doi":null,"title":"Cloning, expression, and functional characterization of in-house prepared human leukemia inhibitory factor.","display_name":"Cloning, expression, and functional characterization of in-house prepared human leukemia inhibitory factor.","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W2102825615","mag":"2102825615","pmid":"https://pubmed.ncbi.nlm.nih.gov/23862122","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3712781"},"language":"en","primary_location":{"id":"pmh:oai:europepmc.org:2708152","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3712781","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3712781","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026328934","display_name":"Hassan Rassouli","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I202419968","display_name":"Royan Institute","ror":"https://ror.org/02exhb815","country_code":"IR","type":"nonprofit","lineage":["https://openalex.org/I202419968"]},{"id":"https://openalex.org/I2802694670","display_name":"Academic Center for Education, Culture and Research","ror":"https://ror.org/0126z4b94","country_code":"IR","type":"nonprofit","lineage":["https://openalex.org/I2802694670"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Hassan Rassouli","raw_affiliation_strings":["1. Department of Molecular Systems Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1. Department of Molecular Systems Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran","institution_ids":["https://openalex.org/I202419968","https://openalex.org/I2802694670"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113830556","display_name":"Shiva Nemati","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I202419968","display_name":"Royan Institute","ror":"https://ror.org/02exhb815","country_code":"IR","type":"nonprofit","lineage":["https://openalex.org/I202419968"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Shiva Nemati","raw_affiliation_strings":["ROYAN INSTITUTE"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ROYAN INSTITUTE","institution_ids":["https://openalex.org/I202419968"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085241381","display_name":"Siamak Rezaeiani","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Siamak Rezaeiani","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061164618","display_name":"Ali Sayadmanesh","orcid":"https://orcid.org/0000-0003-2338-9840","observed_orcids":["https://orcid.org/0000-0003-2338-9840"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ali Sayadmanesh","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111847501","display_name":"Mohammad Reza Gharaati","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mohammad Reza Gharaati","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001269082","display_name":"Ghasem Hosseini Salekdeh","orcid":"https://orcid.org/0000-0002-5124-4721","observed_orcids":["https://orcid.org/0000-0002-5124-4721"]},"institutions":[{"id":"https://openalex.org/I202419968","display_name":"Royan Institute","ror":"https://ror.org/02exhb815","country_code":"IR","type":"nonprofit","lineage":["https://openalex.org/I202419968"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Ghasem Hosseini Salekdeh","raw_affiliation_strings":["ROYAN INSTITUTE"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ROYAN INSTITUTE","institution_ids":["https://openalex.org/I202419968"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091186278","display_name":"Hossein Baharvand","orcid":"https://orcid.org/0000-0001-6528-3687","observed_orcids":["https://orcid.org/0000-0001-6528-3687"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hossein Baharvand","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5031627943","display_name":"Hamid Gourabi","orcid":"https://orcid.org/0000-0001-7277-4898","observed_orcids":["https://orcid.org/0000-0001-7277-4898"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hamid Gourabi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.11,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.46026223,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"15","issue":"2","first_page":"190","last_page":"7"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9810000061988831,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9810000061988831,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11503","display_name":"Cytokine Signaling Pathways and Interactions","score":0.005400000140070915,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.003599999938160181,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/leukemia-inhibitory-factor","display_name":"leukemia inhibitory factor","score":1.0},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8899999856948853},{"id":"https://openalex.org/keywords/mouse-embryonic-stem-cells","display_name":"mouse embryonic stem cells","score":0.7599999904632568},{"id":"https://openalex.org/keywords/induced-pluripotent-stem-cells","display_name":"induced pluripotent stem cells","score":1.0},{"id":"https://openalex.org/keywords/stem-cell-maintenance","display_name":"stem cell maintenance","score":0.6859999895095825},{"id":"https://openalex.org/keywords/plasmid-cloning","display_name":"plasmid cloning","score":0.6480000019073486},{"id":"https://openalex.org/keywords/functional-characterization","display_name":"functional characterization","score":0.550000011920929}],"concepts":[{"id":"https://openalex.org/C27284151","wikidata":"https://www.wikidata.org/wiki/Q6534502","display_name":"Leukemia inhibitory factor","level":4,"score":0.842796802520752},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7896300554275513},{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.7079703211784363},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.6299226880073547},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.5559951663017273},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5167180895805359},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.48068150877952576},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.4541676342487335},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.44637027382850647},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.4426991045475006},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.42915716767311096},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.20419231057167053},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.18092772364616394},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.09509244561195374},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07820725440979004},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[],"locations_count":6,"locations":[{"id":"pmh:oai:europepmc.org:2708152","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3712781","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:3712781","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3712781","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell J","raw_type":"Text"},{"id":"pmid:23862122","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23862122","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell journal","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.777.9755","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.777.9755","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/8c/63/Cell-J-15-190.PMC3712781.pdf","raw_type":"text"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.864.5580","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.864.5580","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.sid.ir/en/VEWSSID/J_pdf/82320135813.pdf","raw_type":"text"},{"id":"pmh:oai:doaj.org/article:a816235b382f4a909f4520d4901a1a11","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/a816235b382f4a909f4520d4901a1a11","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Cell Journal, Vol 15, Iss 2, Pp 190-197 (2013)","raw_type":"article"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:2708152","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3712781","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9391000270843506}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W1482979909","https://openalex.org/W1539756908","https://openalex.org/W1546645929","https://openalex.org/W1963495927","https://openalex.org/W1968199996","https://openalex.org/W1976076317","https://openalex.org/W1978502066","https://openalex.org/W1987718270","https://openalex.org/W1990050578","https://openalex.org/W1994006339","https://openalex.org/W1994806718","https://openalex.org/W1995176683","https://openalex.org/W2008244502","https://openalex.org/W2012160384","https://openalex.org/W2015352093","https://openalex.org/W2018855387","https://openalex.org/W2037342213","https://openalex.org/W2050632125","https://openalex.org/W2053576571","https://openalex.org/W2053633009","https://openalex.org/W2070427174","https://openalex.org/W2073219775","https://openalex.org/W2085647104","https://openalex.org/W2100478793","https://openalex.org/W2105361655","https://openalex.org/W2107927066","https://openalex.org/W2119449055","https://openalex.org/W2159949202","https://openalex.org/W2161045200","https://openalex.org/W2171493548","https://openalex.org/W2335660413","https://openalex.org/W2404979076"],"related_works":["https://openalex.org/W1963495927","https://openalex.org/W2387432312","https://openalex.org/W2463102383","https://openalex.org/W2891936237","https://openalex.org/W2047308052","https://openalex.org/W4220749617","https://openalex.org/W2803122749","https://openalex.org/W2036210696","https://openalex.org/W2119449055","https://openalex.org/W2016014660"],"abstract_inverted_index":{"OBJECTIVE:":[0],"Leukemia":[1],"inhibitory":[2],"factor":[3,40],"(LIF)":[4],"plays":[5],"important":[6],"roles":[7],"in":[8,56,96,131,160,165,174],"cellular":[9],"proliferation,":[10],"growth":[11,39],"promotion":[12],"and":[13,31,50,156,163,179],"differentiation":[14],"of":[15,18,44,99,147],"various":[16],"types":[17],"target":[19],"cells.":[20],"In":[21,64],"addition,":[22],"LIF":[23,34],"influences":[24],"bone":[25],"metabolism,":[26],"cachexia,":[27],"neural":[28],"development,":[29],"embryogenesis":[30],"inflammation.":[32],"Human":[33],"(hLIF)":[35],"is":[36,153],"an":[37],"essential":[38],"for":[41],"the":[42,72,86,91,97,116],"maintenance":[43],"mouse":[45,161],"embryonic":[46],"stem":[47,53],"cells":[48,54,113],"(ESCs)":[49],"induced":[51],"pluripotent":[52],"(iPSCs)":[55],"a":[57,78,100,125,148],"pluripotent,":[58],"undifferentiated":[59],"state.":[60],"MATERIALS":[61],"AND":[62],"METHODS:":[63],"this":[65],"experimental":[66],"study,":[67],"we":[68],"cloned":[69],"hLIF":[70,82,129,150],"into":[71,85,105],"pENTR-D/":[73],"TOPO":[74,79],"entry":[75],"vector":[76,89],"by":[77,90],"reaction.":[80],"Next,":[81],"was":[83,103],"subcloned":[84],"pDEST17":[87],"destination":[88],"LR":[92],"reaction,":[93],"which":[94],"resulted":[95],"production":[98],"construct":[101],"that":[102,134,152],"transferred":[104],"E.":[106,132],"coli":[107,133],"strain":[108],"Rosetta-gami\u2122":[109],"2(DE3)":[110],"pLacI":[111],"competent":[112],"to":[114],"produce":[115],"His6-hLIF":[117],"fusion":[118],"protein.":[119],"RESULTS:":[120],"This":[121,139],"straightforward":[122],"method":[123],"produced":[124,178],"biologically":[126,154],"active":[127,155],"recombinant":[128],"protein":[130,151],"has":[135,141],"long-term":[136],"storage":[137],"ability.":[138],"procedure":[140],"provided":[142],"rapid,":[143],"cost":[144],"effective":[145],"purification":[146],"soluble":[149],"functional":[157],"as":[158],"measured":[159],"ESCs":[162],"iPSCs":[164],"vitro.":[166],"CONCLUSION:":[167],"Our":[168],"results":[169],"showed":[170],"no":[171],"significant":[172],"differences":[173],"function":[175],"between":[176],"laboratory":[177],"commercialized":[180],"hLIF.":[181]},"counts_by_year":[{"year":2020,"cited_by_count":3},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1}],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2016-06-24T00:00:00"}