{"id":"https://openalex.org/W2102641990","doi":"https://doi.org/10.1002/prot.20618","title":"Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast","display_name":"Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast","publication_year":2005,"publication_date":"2005-12-14","ids":{"openalex":"https://openalex.org/W2102641990","doi":"https://doi.org/10.1002/prot.20618","mag":"2102641990","pmid":"https://pubmed.ncbi.nlm.nih.gov/16355407"},"language":"en","primary_location":{"id":"doi:10.1002/prot.20618","doi":"https://doi.org/10.1002/prot.20618","is_oa":false,"landing_page_url":"https://doi.org/10.1002/prot.20618","pdf_url":null,"source":{"id":"https://openalex.org/S121161810","display_name":"Proteins Structure Function and Bioinformatics","issn_l":"0887-3585","issn":["0887-3585","1097-0134"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proteins: Structure, Function, and Bioinformatics","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061875704","display_name":"Yong\u2010Sung Kim","orcid":"https://orcid.org/0000-0003-2673-1509","observed_orcids":["https://orcid.org/0000-0003-2673-1509"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yong\u2010Sung Kim","raw_affiliation_strings":["Division of Biological Engineering, Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biological Engineering, Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015652615","display_name":"Rashna Bhandari","orcid":"https://orcid.org/0000-0003-3101-0204","observed_orcids":["https://orcid.org/0000-0003-3101-0204"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I95457486","display_name":"University of California, Berkeley","ror":"https://ror.org/01an7q238","country_code":"US","type":"education","lineage":["https://openalex.org/I95457486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rashna Bhandari","raw_affiliation_strings":["Howard Hughes Medical Institute, Department of Molecular and Cell Biology and Department of Chemistry, University of California, Berkeley, California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Howard Hughes Medical Institute, Department of Molecular and Cell Biology and Department of Chemistry, University of California, Berkeley, California","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I95457486"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058204963","display_name":"Jennifer R. Cochran","orcid":"https://orcid.org/0000-0002-6532-9873","observed_orcids":["https://orcid.org/0000-0002-6532-9873"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jennifer R. Cochran","raw_affiliation_strings":["Division of Biological Engineering and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biological Engineering and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038330393","display_name":"John Kuriyan","orcid":"https://orcid.org/0000-0002-4414-5477","observed_orcids":["https://orcid.org/0000-0002-4414-5477"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I95457486","display_name":"University of California, Berkeley","ror":"https://ror.org/01an7q238","country_code":"US","type":"education","lineage":["https://openalex.org/I95457486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John Kuriyan","raw_affiliation_strings":["Howard Hughes Medical Institute, Department of Molecular and Cell Biology and Department of Chemistry, University of California, Berkeley, California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Howard Hughes Medical Institute, Department of Molecular and Cell Biology and Department of Chemistry, University of California, Berkeley, California","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I95457486"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111326412","display_name":"Karl Dane Wittrup","orcid":"https://orcid.org/0000-0003-2398-5896","observed_orcids":["https://orcid.org/0000-0003-2398-5896"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"K. Dane Wittrup","raw_affiliation_strings":["Division of Biological Engineering and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biological Engineering and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts","institution_ids":["https://openalex.org/I63966007"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5111326412"],"corresponding_institution_ids":["https://openalex.org/I63966007"],"apc_list":{"value":4550,"currency":"USD","value_usd":4550},"apc_paid":null,"fwci":1.6519,"has_fulltext":false,"cited_by_count":69,"citation_normalized_percentile":{"value":0.82060402,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"62","issue":"4","first_page":"1026","last_page":"1035"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9398999810218811,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9398999810218811,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.01510000042617321,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.005200000014156103,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/epidermal-growth-factor-receptor","display_name":"epidermal growth factor receptor","score":0.9980000257492065},{"id":"https://openalex.org/keywords/extracellular-domain","display_name":"extracellular domain","score":0.972000002861023},{"id":"https://openalex.org/keywords/directed-evolution","display_name":"directed evolution","score":0.9430000185966492},{"id":"https://openalex.org/keywords/yeast-surface-display","display_name":"yeast surface display","score":0.9919999837875366},{"id":"https://openalex.org/keywords/egf-binding","display_name":"EGF binding","score":0.5730000138282776},{"id":"https://openalex.org/keywords/autophosphorylation","display_name":"autophosphorylation","score":0.8980000019073486},{"id":"https://openalex.org/keywords/mapk-signaling","display_name":"MAPK signaling","score":0.9959999918937683},{"id":"https://openalex.org/keywords/plasma-membrane-localization","display_name":"plasma membrane localization","score":0.6980000138282776}],"concepts":[{"id":"https://openalex.org/C150109051","wikidata":"https://www.wikidata.org/wiki/Q3630494","display_name":"Autophosphorylation","level":4,"score":0.7445015907287598},{"id":"https://openalex.org/C2779438470","wikidata":"https://www.wikidata.org/wiki/Q424401","display_name":"Epidermal growth factor receptor","level":3,"score":0.7291871309280396},{"id":"https://openalex.org/C2776362946","wikidata":"https://www.wikidata.org/wiki/Q424374","display_name":"Epidermal growth factor","level":3,"score":0.7007232308387756},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.6320282220840454},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.6218434572219849},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.621568500995636},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.5826501250267029},{"id":"https://openalex.org/C108625454","wikidata":"https://www.wikidata.org/wiki/Q187126","display_name":"Glycoprotein","level":2,"score":0.546831488609314},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5464722514152527},{"id":"https://openalex.org/C115456853","wikidata":"https://www.wikidata.org/wiki/Q17917132","display_name":"ERBB3","level":4,"score":0.5187130570411682},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.505748987197876},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.4709164798259735},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.4698311984539032},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.4635523855686188},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.43248021602630615},{"id":"https://openalex.org/C197462201","wikidata":"https://www.wikidata.org/wiki/Q2476074","display_name":"Cell surface receptor","level":3,"score":0.4318041205406189},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.1647219955921173},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.13522157073020935},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1328393816947937},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.13120591640472412},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.08811768889427185}],"mesh":[{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004815","descriptor_name":"Epidermal Growth Factor","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004815","descriptor_name":"Epidermal Growth Factor","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004815","descriptor_name":"Epidermal Growth Factor","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016366","descriptor_name":"Open Reading Frames","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017433","descriptor_name":"Protein Structure, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019943","descriptor_name":"Amino Acid Substitution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1002/prot.20618","doi":"https://doi.org/10.1002/prot.20618","is_oa":false,"landing_page_url":"https://doi.org/10.1002/prot.20618","pdf_url":null,"source":{"id":"https://openalex.org/S121161810","display_name":"Proteins Structure Function and Bioinformatics","issn_l":"0887-3585","issn":["0887-3585","1097-0134"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proteins: Structure, Function, and Bioinformatics","raw_type":"journal-article"},{"id":"pmid:16355407","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16355407","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proteins","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.486.352","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.486.352","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://ws.ajou.ac.kr/~bioeng/papers/proteins_2006.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5592985994","display_name":"Engineered Antibody EGFR Antagonist Cancer Therapeutics","funder_award_id":"R01CA096504","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":44,"referenced_works":["https://openalex.org/W51995314","https://openalex.org/W135877420","https://openalex.org/W1498780407","https://openalex.org/W1521840182","https://openalex.org/W1560333424","https://openalex.org/W1570686199","https://openalex.org/W1767475163","https://openalex.org/W1966658428","https://openalex.org/W1978877135","https://openalex.org/W1988037036","https://openalex.org/W1990030711","https://openalex.org/W1990566414","https://openalex.org/W1990789777","https://openalex.org/W1995337883","https://openalex.org/W2002908987","https://openalex.org/W2023397454","https://openalex.org/W2027469872","https://openalex.org/W2029999856","https://openalex.org/W2030464104","https://openalex.org/W2031546084","https://openalex.org/W2031810447","https://openalex.org/W2033488181","https://openalex.org/W2034287217","https://openalex.org/W2035333743","https://openalex.org/W2043911313","https://openalex.org/W2044622948","https://openalex.org/W2055598450","https://openalex.org/W2058620780","https://openalex.org/W2062542077","https://openalex.org/W2065870425","https://openalex.org/W2071086763","https://openalex.org/W2080904861","https://openalex.org/W2095027173","https://openalex.org/W2099580557","https://openalex.org/W2099831601","https://openalex.org/W2103472483","https://openalex.org/W2103551836","https://openalex.org/W2122508875","https://openalex.org/W2133671622","https://openalex.org/W2143600397","https://openalex.org/W2149939700","https://openalex.org/W2153133904","https://openalex.org/W2159275729","https://openalex.org/W2160112128"],"related_works":["https://openalex.org/W7099514306","https://openalex.org/W2160112128","https://openalex.org/W4401446621","https://openalex.org/W2372328787","https://openalex.org/W2430461151","https://openalex.org/W968076459","https://openalex.org/W1592022389","https://openalex.org/W1990566414","https://openalex.org/W2774556349","https://openalex.org/W2140303505"],"abstract_inverted_index":{"The":[0],"extracellular":[1],"domain":[2],"of":[3,101],"epidermal":[4,52],"growth":[5,53],"factor":[6],"receptor":[7,105],"(EGFR-ECD)":[8],"has":[9],"been":[10],"engineered":[11],"through":[12],"directed":[13],"evolution":[14],"and":[15,30,50,55,78,85,88,112],"yeast":[16,108],"surface":[17],"display":[18],"using":[19],"conformationally-specific":[20,48],"monoclonal":[21],"antibodies":[22],"(mAbs)":[23],"as":[24,73],"screening":[25],"probes":[26],"for":[27,109],"proper":[28],"folding":[29],"functional":[31],"expression":[32],"in":[33,82,94,107],"Saccharomyces":[34],"cerevisiae.":[35],"An":[36],"EGFR":[37,66],"mutant":[38,69],"with":[39,62],"four":[40],"amino":[41],"acid":[42],"changes":[43],"exhibited":[44],"binding":[45],"to":[46],"the":[47,68,91],"mAbs":[49],"human":[51],"factor,":[54],"showed":[56],"increased":[57],"soluble":[58],"secretion":[59],"efficiency":[60],"compared":[61],"wild-type":[63],"EGFR.":[64],"Full-length":[65],"containing":[67],"EGFR-ECD":[70],"was":[71,86],"functional,":[72],"assayed":[74],"by":[75],"EGF-dependent":[76],"autophosphorylation":[77],"intracellular":[79],"MAPK":[80],"signaling":[81],"mammalian":[83,104],"cells,":[84],"expressed":[87],"localized":[89],"at":[90],"plasma":[92],"membrane":[93],"yeast.":[95],"This":[96],"approach":[97],"should":[98],"enable":[99],"engineering":[100],"other":[102],"complex":[103],"glycoproteins":[106],"genetic,":[110],"structural,":[111],"biophysical":[113],"studies.":[114]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":8},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}