{"id":"https://openalex.org/W2102445673","doi":"https://doi.org/10.1073/pnas.89.2.658","title":"A protease responsible for post-translational cleavage of a conserved Asn-Gly linkage in glycinin, the major seed storage protein of soybean","display_name":"A protease responsible for post-translational cleavage of a conserved Asn-Gly linkage in glycinin, the major seed storage protein of soybean","publication_year":1992,"publication_date":"1992-01-15","ids":{"openalex":"https://openalex.org/W2102445673","doi":"https://doi.org/10.1073/pnas.89.2.658","mag":"2102445673","pmid":"https://pubmed.ncbi.nlm.nih.gov/1731337","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/48298"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.89.2.658","doi":"https://doi.org/10.1073/pnas.89.2.658","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.89.2.658","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/48298","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5011286193","display_name":"M. Paul Scott","orcid":"https://orcid.org/0000-0003-0479-4816","observed_orcids":["https://orcid.org/0000-0003-0479-4816"]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. Paul Scott","raw_affiliation_strings":["U.S. Department of Agriculture Agricultural Research Service, Department of Agronomy, Purdue University, West Lafayette, IN 47907"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"U.S. Department of Agriculture Agricultural Research Service, Department of Agronomy, Purdue University, West Lafayette, IN 47907","institution_ids":["https://openalex.org/I1312222531","https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000515613","display_name":"Rudolf Jung","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]},{"id":"https://openalex.org/I4210127902","display_name":"Leibniz-Institut f\u00fcr Pflanzengenetik und Kulturpflanzenforschung (IPK)","ror":"https://ror.org/02skbsp27","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210127902"]}],"countries":["DE","US"],"is_corresponding":false,"raw_author_name":"Rudolf Jung","raw_affiliation_strings":["U.S. Department of Agriculture Agricultural Research Service, Department of Agronomy, Purdue University, West Lafayette, IN 47907","Zentralinstitut f\u00fcr Genetik und Kulturpflanzenforschung, Gatersleben O-4325, Federal Republic of Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"U.S. Department of Agriculture Agricultural Research Service, Department of Agronomy, Purdue University, West Lafayette, IN 47907","institution_ids":["https://openalex.org/I1312222531","https://openalex.org/I219193219"]},{"raw_affiliation_string":"Zentralinstitut f\u00fcr Genetik und Kulturpflanzenforschung, Gatersleben O-4325, Federal Republic of Germany","institution_ids":["https://openalex.org/I4210127902"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038406250","display_name":"Klaus M\u00fcntz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210127902","display_name":"Leibniz-Institut f\u00fcr Pflanzengenetik und Kulturpflanzenforschung (IPK)","ror":"https://ror.org/02skbsp27","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210127902"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Klaus Muntz","raw_affiliation_strings":["Zentralinstitut f\u00fcr Genetik und Kulturpflanzenforschung, Gatersleben O-4325, Federal Republic of Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Zentralinstitut f\u00fcr Genetik und Kulturpflanzenforschung, Gatersleben O-4325, Federal Republic of Germany","institution_ids":["https://openalex.org/I4210127902"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5082779199","display_name":"Niels Chr. Nielsen","orcid":"https://orcid.org/0000-0003-2978-4366","observed_orcids":["https://orcid.org/0000-0003-2978-4366"]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Niels C. Nielsen","raw_affiliation_strings":["U.S. Department of Agriculture Agricultural Research Service, Department of Agronomy, Purdue University, West Lafayette, IN 47907"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"U.S. Department of Agriculture Agricultural Research Service, Department of Agronomy, Purdue University, West Lafayette, IN 47907","institution_ids":["https://openalex.org/I1312222531","https://openalex.org/I219193219"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":7.3402,"has_fulltext":false,"cited_by_count":133,"citation_normalized_percentile":{"value":0.98124414,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"89","issue":"2","first_page":"658","last_page":"662"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.21559999883174896,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.21559999883174896,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.18639999628067017,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.14069999754428864,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glycinin","display_name":"glycinin","score":0.8349999785423279},{"id":"https://openalex.org/keywords/11s-globulin","display_name":"11S globulin","score":0.722000002861023},{"id":"https://openalex.org/keywords/seed-storage-proteins","display_name":"seed storage proteins","score":0.9079999923706055},{"id":"https://openalex.org/keywords/post-translational-cleavage","display_name":"post-translational cleavage","score":0.8090000152587891},{"id":"https://openalex.org/keywords/protease","display_name":"proteolytic enzymes","score":0.6039999723434448},{"id":"https://openalex.org/keywords/cleavage-specificity","display_name":"cleavage specificity","score":0.7369999885559082},{"id":"https://openalex.org/keywords/soybean","display_name":"soybean","score":0.609000027179718},{"id":"https://openalex.org/keywords/vicia-faba","display_name":"Vicia faba","score":0.9950000047683716},{"id":"https://openalex.org/keywords/protein-assembly","display_name":"protein assembly","score":0.3479999899864197},{"id":"https://openalex.org/keywords/hexamer-formation","display_name":"hexamer formation","score":0.4059999883174896}],"concepts":[{"id":"https://openalex.org/C97564808","wikidata":"https://www.wikidata.org/wiki/Q425526","display_name":"Storage protein","level":3,"score":0.8165558576583862},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.7308378219604492},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7148492932319641},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.6270704865455627},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.597658097743988},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.5056228637695312},{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.49012455344200134},{"id":"https://openalex.org/C2992233211","wikidata":"https://www.wikidata.org/wiki/Q131342","display_name":"Vicia faba","level":2,"score":0.47856929898262024},{"id":"https://openalex.org/C2775895851","wikidata":"https://www.wikidata.org/wiki/Q185906","display_name":"Asparagine","level":3,"score":0.47615504264831543},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.4658421277999878},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.45077207684516907},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4501773715019226},{"id":"https://openalex.org/C2777756961","wikidata":"https://www.wikidata.org/wiki/Q620730","display_name":"Glycine","level":3,"score":0.4135134816169739},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3856886923313141},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09833040833473206},{"id":"https://openalex.org/C43369102","wikidata":"https://www.wikidata.org/wiki/Q2307625","display_name":"Fracture (geology)","level":2,"score":0.0},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000097793","descriptor_name":"Legumins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005916","descriptor_name":"Globulins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005916","descriptor_name":"Globulins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011499","descriptor_name":"Protein Processing, Post-Translational","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013025","descriptor_name":"Glycine max","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D013025","descriptor_name":"Glycine max","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014674","descriptor_name":"Plant Proteins, Dietary","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014674","descriptor_name":"Plant Proteins, Dietary","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D030262","descriptor_name":"Soybean Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1073/pnas.89.2.658","doi":"https://doi.org/10.1073/pnas.89.2.658","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.89.2.658","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:48298","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/48298","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmid:1731337","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1731337","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:48298","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/48298","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Zero hunger","score":0.6875,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W1544745708","https://openalex.org/W1549946077","https://openalex.org/W1594700207","https://openalex.org/W1596599934","https://openalex.org/W2002250628","https://openalex.org/W2012215782","https://openalex.org/W2013484763","https://openalex.org/W2021842964","https://openalex.org/W2066778381","https://openalex.org/W2081371452","https://openalex.org/W2113772935","https://openalex.org/W2117689741","https://openalex.org/W2122912323","https://openalex.org/W2125372295","https://openalex.org/W2128809047","https://openalex.org/W2135368954","https://openalex.org/W2159792557","https://openalex.org/W2167190605"],"related_works":["https://openalex.org/W3104265963","https://openalex.org/W2086051151","https://openalex.org/W1987907371","https://openalex.org/W2135368954","https://openalex.org/W2123052715","https://openalex.org/W4390458386","https://openalex.org/W2112640697","https://openalex.org/W2026505245","https://openalex.org/W2409131265","https://openalex.org/W1591134545"],"abstract_inverted_index":{"The":[0,121],"assembly":[1],"of":[2,17,29,42,70,78,141,155,172],"11S":[3,66],"globulin":[4],"seed":[5,67],"storage":[6,68],"proteins":[7,69,100],"in":[8,12,44,50,56,102,113,176],"plants":[9],"is":[10,37,54,159],"regulated":[11],"part":[13],"by":[14],"the":[15,40,76,79,96,114,118,142,151,156],"activity":[16,53,126],"a":[18,129,160,169],"protease":[19,122],"that":[20,62,89],"cleaves":[21,64],"between":[22],"asparagine":[23],"and":[24,34,72,91,153,168],"glycine":[25],"residues.":[26],"Post-translational":[27],"cleavage":[28,115,146],"subunit":[30,144],"precursors":[31],"into":[32,48,75],"acidic":[33,165],"basic":[35],"polypeptides":[36,77],"associated":[38],"with":[39,163],"ability":[41],"subunits":[43],"trimers":[45],"to":[46,87],"aggregate":[47],"hexamers":[49],"vitro.":[51],"An":[52],"present":[55],"extracts":[57],"from":[58],"immature":[59,65],"soybean":[60,71],"seeds":[61],"specifically":[63],"Vicia":[73],"faba":[74],"mature":[80],"proteins.":[81],"Sequence":[82],"microanalysis":[83],"has":[84],"been":[85],"used":[86,105],"demonstrate":[88],"proglycinin":[90],"prolegumin":[92],"are":[93,104],"cut":[94],"at":[95],"legitimate":[97],"site":[98,116],"when":[99],"synthesized":[101],"vitro":[103],"as":[106],"substrates.":[107],"A":[108],"single":[109],"amino":[110,136],"acid":[111],"change":[112],"renders":[117],"substrate":[119],"uncleavable.":[120],"responsible":[123],"for":[124],"this":[125],"also":[127],"hydrolyzes":[128],"synthetic":[130],"octapeptide":[131],"whose":[132],"sequence":[133],"reproduces":[134],"four":[135],"acids":[137],"on":[138],"either":[139],"side":[140],"glycinin":[143],"G4":[145],"site.":[147],"This":[148],"assay":[149],"permitted":[150],"purification":[152],"characterization":[154],"protease.":[157],"It":[158],"glycosylated":[161],"enzyme":[162],"an":[164],"pH":[166],"optimum":[167],"molecular":[170],"mass":[171],"about":[173],"45":[174],"kDa":[175],"solution.":[177]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":4}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}