{"id":"https://openalex.org/W2100535792","doi":"https://doi.org/10.1016/s0014-5793(98)01096-5","title":"Ribozyme processed tRNA transcripts with unfriendly internal promoter for T7 RNA polymerase: production and activity","display_name":"Ribozyme processed tRNA transcripts with unfriendly internal promoter for T7 RNA polymerase: production and activity","publication_year":1998,"publication_date":"1998-09-25","ids":{"openalex":"https://openalex.org/W2100535792","doi":"https://doi.org/10.1016/s0014-5793(98)01096-5","mag":"2100535792","pmid":"https://pubmed.ncbi.nlm.nih.gov/9771901"},"language":"en","primary_location":{"id":"doi:10.1016/s0014-5793(98)01096-5","doi":"https://doi.org/10.1016/s0014-5793(98)01096-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0014-5793(98)01096-5","pdf_url":null,"source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5053268831","display_name":"Pierre Fechter","orcid":"https://orcid.org/0000-0001-5912-361X","observed_orcids":["https://orcid.org/0000-0001-5912-361X"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210128885","display_name":"Architecture et R\u00e9activit\u00e9 de l'arN","ror":"https://ror.org/03xmjtz19","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210096427","https://openalex.org/I4210128885","https://openalex.org/I68947357"]},{"id":"https://openalex.org/I4210158086","display_name":"Institut de Biologie Mol\u00e9culaire et Cellulaire","ror":"https://ror.org/05qpmg879","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210158086","https://openalex.org/I68947357"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Pierre Fechter","raw_affiliation_strings":["UPR 9002 du CNRS, Institut de Biologie Mol\u00e9culaire et Cellulaire, Strasbourg, France","ARN - Architecture et R\u00e9activit\u00e9 de l'ARN (Institut de Biologie Mol\u00e9culaire et Cellulaire 15 rue Ren\u00e9 Descartes 67084 STRASBOURG Cedex - France)","UPR 9002 du CNRS, Institut de Biologie Mol\u00e9culaire et Cellulaire, 15, rue Ren\u00e9 Descartes, 67084 Strasbourg Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UPR 9002 du CNRS, Institut de Biologie Mol\u00e9culaire et Cellulaire, Strasbourg, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210128885"]},{"raw_affiliation_string":"ARN - Architecture et R\u00e9activit\u00e9 de l'ARN (Institut de Biologie Mol\u00e9culaire et Cellulaire 15 rue Ren\u00e9 Descartes 67084 STRASBOURG Cedex - France)","institution_ids":["https://openalex.org/I4210128885","https://openalex.org/I4210158086"]},{"raw_affiliation_string":"UPR 9002 du CNRS, Institut de Biologie Mol\u00e9culaire et Cellulaire, 15, rue Ren\u00e9 Descartes, 67084 Strasbourg Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210128885"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023344474","display_name":"Jo\u00eblle Rudinger\u2010Thirion","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210128885","display_name":"Architecture et R\u00e9activit\u00e9 de l'arN","ror":"https://ror.org/03xmjtz19","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210096427","https://openalex.org/I4210128885","https://openalex.org/I68947357"]},{"id":"https://openalex.org/I4210158086","display_name":"Institut de Biologie Mol\u00e9culaire et Cellulaire","ror":"https://ror.org/05qpmg879","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210158086","https://openalex.org/I68947357"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Jo\u00eblle Rudinger","raw_affiliation_strings":["UPR 9002 du CNRS, Institut de Biologie Mole\u0301culaire et Cellulaire, 15 Rue Rene\u0301 Descartes, 67084 Strasbourg Cedex, France","ARN - Architecture et R\u00e9activit\u00e9 de l'ARN (Institut de Biologie Mol\u00e9culaire et Cellulaire 15 rue Ren\u00e9 Descartes 67084 STRASBOURG Cedex - France)","UPR 9002 du CNRS, Institut de Biologie Mol\u00e9culaire et Cellulaire, 15, rue Ren\u00e9 Descartes, 67084 Strasbourg Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UPR 9002 du CNRS, Institut de Biologie Mole\u0301culaire et Cellulaire, 15 Rue Rene\u0301 Descartes, 67084 Strasbourg Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210128885"]},{"raw_affiliation_string":"ARN - Architecture et R\u00e9activit\u00e9 de l'ARN (Institut de Biologie Mol\u00e9culaire et Cellulaire 15 rue Ren\u00e9 Descartes 67084 STRASBOURG Cedex - France)","institution_ids":["https://openalex.org/I4210128885","https://openalex.org/I4210158086"]},{"raw_affiliation_string":"UPR 9002 du CNRS, Institut de Biologie Mol\u00e9culaire et Cellulaire, 15, rue Ren\u00e9 Descartes, 67084 Strasbourg Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210128885"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054694568","display_name":"Richard Gieg\u00e9","orcid":"https://orcid.org/0000-0001-9662-1482","observed_orcids":["https://orcid.org/0000-0001-9662-1482"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210128885","display_name":"Architecture et R\u00e9activit\u00e9 de l'arN","ror":"https://ror.org/03xmjtz19","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210096427","https://openalex.org/I4210128885","https://openalex.org/I68947357"]},{"id":"https://openalex.org/I4210158086","display_name":"Institut de Biologie Mol\u00e9culaire et Cellulaire","ror":"https://ror.org/05qpmg879","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210158086","https://openalex.org/I68947357"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Richard Gieg\u00e9","raw_affiliation_strings":["UPR 9002 du CNRS, Institut de Biologie Mole\u0301culaire et Cellulaire, 15 Rue Rene\u0301 Descartes, 67084 Strasbourg Cedex, France","ARN - Architecture et R\u00e9activit\u00e9 de l'ARN (Institut de Biologie Mol\u00e9culaire et Cellulaire 15 rue Ren\u00e9 Descartes 67084 STRASBOURG Cedex - France)","UPR 9002 du CNRS, Institut de Biologie Mol\u00e9culaire et Cellulaire, 15, rue Ren\u00e9 Descartes, 67084 Strasbourg Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UPR 9002 du CNRS, Institut de Biologie Mole\u0301culaire et Cellulaire, 15 Rue Rene\u0301 Descartes, 67084 Strasbourg Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210128885"]},{"raw_affiliation_string":"ARN - Architecture et R\u00e9activit\u00e9 de l'ARN (Institut de Biologie Mol\u00e9culaire et Cellulaire 15 rue Ren\u00e9 Descartes 67084 STRASBOURG Cedex - France)","institution_ids":["https://openalex.org/I4210128885","https://openalex.org/I4210158086"]},{"raw_affiliation_string":"UPR 9002 du CNRS, Institut de Biologie Mol\u00e9culaire et Cellulaire, 15, rue Ren\u00e9 Descartes, 67084 Strasbourg Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210128885"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018545943","display_name":"Anne Th\u00e9obald\u2010Dietrich","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210128885","display_name":"Architecture et R\u00e9activit\u00e9 de l'arN","ror":"https://ror.org/03xmjtz19","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210096427","https://openalex.org/I4210128885","https://openalex.org/I68947357"]},{"id":"https://openalex.org/I4210158086","display_name":"Institut de Biologie Mol\u00e9culaire et Cellulaire","ror":"https://ror.org/05qpmg879","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210158086","https://openalex.org/I68947357"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Anne Th\u00e9obald-Dietrich","raw_affiliation_strings":["UPR 9002 du CNRS, Institut de Biologie Mole\u0301culaire et Cellulaire, 15 Rue Rene\u0301 Descartes, 67084 Strasbourg Cedex, France","ARN - Architecture et R\u00e9activit\u00e9 de l'ARN (Institut de Biologie Mol\u00e9culaire et Cellulaire 15 rue Ren\u00e9 Descartes 67084 STRASBOURG Cedex - France)","UPR 9002 du CNRS, Institut de Biologie Mol\u00e9culaire et Cellulaire, 15, rue Ren\u00e9 Descartes, 67084 Strasbourg Cedex, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UPR 9002 du CNRS, Institut de Biologie Mole\u0301culaire et Cellulaire, 15 Rue Rene\u0301 Descartes, 67084 Strasbourg Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210128885"]},{"raw_affiliation_string":"ARN - Architecture et R\u00e9activit\u00e9 de l'ARN (Institut de Biologie Mol\u00e9culaire et Cellulaire 15 rue Ren\u00e9 Descartes 67084 STRASBOURG Cedex - France)","institution_ids":["https://openalex.org/I4210128885","https://openalex.org/I4210158086"]},{"raw_affiliation_string":"UPR 9002 du CNRS, Institut de Biologie Mol\u00e9culaire et Cellulaire, 15, rue Ren\u00e9 Descartes, 67084 Strasbourg Cedex, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210128885"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5018545943"],"corresponding_institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210128885","https://openalex.org/I4210158086"],"apc_list":{"value":3980,"currency":"USD","value_usd":3980},"apc_paid":null,"fwci":1.3564,"has_fulltext":false,"cited_by_count":144,"citation_normalized_percentile":{"value":0.80133855,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":92,"max":99},"biblio":{"volume":"436","issue":"1","first_page":"99","last_page":"103"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/trna-transcription","display_name":"tRNA gene transcription","score":0.7260000109672546},{"id":"https://openalex.org/keywords/t7-rna-polymerase","display_name":"T7 RNA polymerase","score":0.9010000228881836},{"id":"https://openalex.org/keywords/hammerhead-ribozyme","display_name":"hammerhead ribozyme","score":0.9789999723434448},{"id":"https://openalex.org/keywords/5-terminal-nucleotides","display_name":"5'-terminal nucleotides","score":0.6949999928474426},{"id":"https://openalex.org/keywords/trnaasp","display_name":"tRNAAsp","score":0.9940000176429749},{"id":"https://openalex.org/keywords/yeast-trna","display_name":"yeast tRNA","score":0.824999988079071},{"id":"https://openalex.org/keywords/aminoacylation","display_name":"aminoacylation","score":0.6729999780654907},{"id":"https://openalex.org/keywords/in-vitro-transcription","display_name":"in vitro transcription","score":0.6150000095367432}],"concepts":[{"id":"https://openalex.org/C76346623","wikidata":"https://www.wikidata.org/wiki/Q205858","display_name":"Ribozyme","level":4,"score":0.7329399585723877},{"id":"https://openalex.org/C2780048736","wikidata":"https://www.wikidata.org/wiki/Q418713","display_name":"T7 RNA polymerase","level":5,"score":0.6064870357513428},{"id":"https://openalex.org/C153957851","wikidata":"https://www.wikidata.org/wiki/Q201448","display_name":"Transfer RNA","level":4,"score":0.5930335521697998},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.5609618425369263},{"id":"https://openalex.org/C64350747","wikidata":"https://www.wikidata.org/wiki/Q15334993","display_name":"RNA polymerase II","level":5,"score":0.5282917022705078},{"id":"https://openalex.org/C2779792011","wikidata":"https://www.wikidata.org/wiki/Q3817690","display_name":"Hammerhead ribozyme","level":5,"score":0.5046428442001343},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4854905903339386},{"id":"https://openalex.org/C70916841","wikidata":"https://www.wikidata.org/wiki/Q3501658","display_name":"RNA polymerase III","level":5,"score":0.473189115524292},{"id":"https://openalex.org/C172768829","wikidata":"https://www.wikidata.org/wiki/Q14817959","display_name":"Transcription factor II D","level":5,"score":0.46558183431625366},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4631340801715851},{"id":"https://openalex.org/C25281209","wikidata":"https://www.wikidata.org/wiki/Q3502188","display_name":"RNA polymerase I","level":5,"score":0.4412260055541992},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.43460118770599365},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.38046330213546753},{"id":"https://openalex.org/C2776449523","wikidata":"https://www.wikidata.org/wiki/Q272631","display_name":"RNA polymerase","level":4,"score":0.36930662393569946},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2734120190143585},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.27031975984573364},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.25982511043548584},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.16938289999961853},{"id":"https://openalex.org/C2776441376","wikidata":"https://www.wikidata.org/wiki/Q165028","display_name":"Bacteriophage","level":4,"score":0.0},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D001671","descriptor_name":"Biochemistry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012321","descriptor_name":"DNA-Directed RNA Polymerases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012321","descriptor_name":"DNA-Directed RNA Polymerases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012365","descriptor_name":"RNA, Transfer, Tyr","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012365","descriptor_name":"RNA, Transfer, Tyr","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012365","descriptor_name":"RNA, Transfer, Tyr","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016337","descriptor_name":"RNA, Catalytic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016337","descriptor_name":"RNA, Catalytic","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/s0014-5793(98)01096-5","doi":"https://doi.org/10.1016/s0014-5793(98)01096-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0014-5793(98)01096-5","pdf_url":null,"source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},{"id":"pmid:9771901","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9771901","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS letters","raw_type":"Journal Article"},{"id":"pmh:oai:HAL:hal-05255738v1","doi":null,"is_oa":false,"landing_page_url":"https://univoak.hal.science/hal-05255738","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://febs.onlinelibrary.wiley.com/doi/full/10.1016/S0014-5793%2898%2901096-5","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":16,"referenced_works":["https://openalex.org/W111642070","https://openalex.org/W1498439983","https://openalex.org/W1596784535","https://openalex.org/W1975152498","https://openalex.org/W1975372074","https://openalex.org/W2002841189","https://openalex.org/W2006145675","https://openalex.org/W2017669909","https://openalex.org/W2037356132","https://openalex.org/W2039733241","https://openalex.org/W2040179169","https://openalex.org/W2042649211","https://openalex.org/W2084476356","https://openalex.org/W2085272777","https://openalex.org/W2113679073","https://openalex.org/W4234879553"],"related_works":["https://openalex.org/W2470171919","https://openalex.org/W1971530826","https://openalex.org/W2053064002","https://openalex.org/W3087548219","https://openalex.org/W403239842","https://openalex.org/W2082069932","https://openalex.org/W7095841144","https://openalex.org/W2415712671","https://openalex.org/W7099860612","https://openalex.org/W1975705587"],"abstract_inverted_index":{"A":[0],"limitation":[1],"for":[2,10,81,93],"a":[3,35,42,59,68],"universal":[4],"use":[5],"of":[6,19,25,53,65,83,124],"T7":[7,44],"RNA":[8,45],"polymerase":[9,46],"in":[11,16,113],"vitro":[12],"tRNA":[13,70],"transcription":[14,82],"lies":[15],"the":[17,20,26,49,62,74,99,103],"nature":[18],"often":[21],"unfavorable":[22],"5'-terminal":[23],"sequence":[24,38],"gene":[27],"to":[28,58,122],"be":[29],"transcribed.":[30],"To":[31],"overcome":[32],"this":[33,54],"drawback,":[34],"hammerhead":[36],"ribozyme":[37],"was":[39,79],"introduced":[40],"between":[41],"strong":[43],"promoter":[47],"and":[48,89,117],"tDNA":[50],"sequence.":[51],"Transcription":[52],"construct":[55],"gives":[56],"rise":[57],"'transzyme'":[60],"molecule,":[61],"autocatalytic":[63],"activity":[64],"which":[66],"liberates":[67],"5'-OH":[69],"transcript":[71],"starting":[72,86],"with":[73,87,96,115],"proper":[75],"nucleotide.":[76],"The":[77],"method":[78,105],"optimized":[80],"yeast":[84,94],"tRNA(Tgammar),":[85],"5'-C1,":[88],"operates":[90],"as":[91],"well":[92],"tRNA(Asp)":[95],"5'-U1.":[97],"Although":[98],"tRNAs":[100],"produced":[101],"by":[102],"transzyme":[104],"are":[106,110],"not":[107],"phosphorylated,":[108],"they":[109],"fully":[111],"active":[112],"aminoacylation":[114],"k(cat)":[116],"Km":[118],"parameters":[119],"quasi":[120],"identical":[121],"those":[123],"their":[125],"phosphorylated":[126],"counterparts.":[127]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":7},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":8},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":6},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":8},{"year":2012,"cited_by_count":6}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}