{"id":"https://openalex.org/W2100511536","doi":"https://doi.org/10.1002/rcm.1290081220","title":"Peptide fingerprints after partial acid hydrolysis: Analysis by matrix\u2010assisted laser desorption/ionization mass spectrometry","display_name":"Peptide fingerprints after partial acid hydrolysis: Analysis by matrix\u2010assisted laser desorption/ionization mass spectrometry","publication_year":1994,"publication_date":"1994-12-01","ids":{"openalex":"https://openalex.org/W2100511536","doi":"https://doi.org/10.1002/rcm.1290081220","mag":"2100511536","pmid":"https://pubmed.ncbi.nlm.nih.gov/7696698"},"language":"en","primary_location":{"id":"doi:10.1002/rcm.1290081220","doi":"https://doi.org/10.1002/rcm.1290081220","is_oa":false,"landing_page_url":"https://doi.org/10.1002/rcm.1290081220","pdf_url":null,"source":{"id":"https://openalex.org/S89689683","display_name":"Rapid Communications in Mass Spectrometry","issn_l":"0951-4198","issn":["0951-4198","1097-0231"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rapid Communications in Mass Spectrometry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087884137","display_name":"Michael D. Knierman","orcid":"https://orcid.org/0000-0001-7427-2269","observed_orcids":["https://orcid.org/0000-0001-7427-2269"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Michael D. Knierman","raw_affiliation_strings":["Monoclonal Antibody/Recombinant Protein Production Facility-PRI-FCRDC, MD 21701","Monoclonal Antibody/Recombinant Protein Production Facility\u2014PRI\u2010FCRDC, PO Box B, Bldg. 1052A, Frederick, MD 21701, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Monoclonal Antibody/Recombinant Protein Production Facility-PRI-FCRDC, MD 21701","institution_ids":[]},{"raw_affiliation_string":"Monoclonal Antibody/Recombinant Protein Production Facility\u2014PRI\u2010FCRDC, PO Box B, Bldg. 1052A, Frederick, MD 21701, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5105466343","display_name":"John E. Coligan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John E. Coligan","raw_affiliation_strings":["Laboratory of Molecular Structure, Bldg 4. Room 413, 9000 Rockville Pike, National Institute of Allergy and Infectious Disease, National Institutes of Health. Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Structure, Bldg 4. Room 413, 9000 Rockville Pike, National Institute of Allergy and Infectious Disease, National Institutes of Health. Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020375596","display_name":"Kenneth C. Parker","orcid":"https://orcid.org/0000-0002-6282-2478","observed_orcids":["https://orcid.org/0000-0002-6282-2478"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kenneth C. Parker","raw_affiliation_strings":["Laboratory of Molecular Structure, Bldg 4. Room 413, 9000 Rockville Pike, National Institute of Allergy and Infectious Disease, National Institutes of Health. Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Structure, Bldg 4. Room 413, 9000 Rockville Pike, National Institute of Allergy and Infectious Disease, National Institutes of Health. Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084513610","display_name":"Brian T. Chait","orcid":"https://orcid.org/0000-0003-3524-557X","observed_orcids":["https://orcid.org/0000-0003-3524-557X"]},"institutions":[{"id":"https://openalex.org/I83399316","display_name":"Rockefeller University","ror":"https://ror.org/0420db125","country_code":"US","type":"education","lineage":["https://openalex.org/I83399316"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"B. T. Chait","raw_affiliation_strings":["The Rockefeller University, New York, NY 10021-6399, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Rockefeller University, New York, NY 10021-6399, USA","institution_ids":["https://openalex.org/I83399316"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5087884137"],"corresponding_institution_ids":[],"apc_list":{"value":4840,"currency":"USD","value_usd":4840},"apc_paid":null,"fwci":1.5878,"has_fulltext":false,"cited_by_count":16,"citation_normalized_percentile":{"value":0.81699746,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"8","issue":"12","first_page":"1007","last_page":"1010"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9804999828338623,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9804999828338623,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.01759999990463257,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/peptide-mass-fingerprinting","display_name":"peptide mass mapping","score":0.9390000104904175},{"id":"https://openalex.org/keywords/partial-acid-hydrolysis","display_name":"partial acid hydrolysis","score":0.972000002861023},{"id":"https://openalex.org/keywords/hydrochloric-acid-hydrolysis","display_name":"hydrochloric acid hydrolysis","score":0.9620000123977661},{"id":"https://openalex.org/keywords/maldi-ms","display_name":"MALDI-MS","score":0.6129999756813049},{"id":"https://openalex.org/keywords/peptide-mapping","display_name":"peptide mapping","score":0.6309999823570251},{"id":"https://openalex.org/keywords/c-terminal-cleavage","display_name":"C-terminal cleavage","score":0.5809999704360962}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9259176254272461},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.8441637754440308},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.7575015425682068},{"id":"https://openalex.org/C28800991","wikidata":"https://www.wikidata.org/wiki/Q15634405","display_name":"Hydrolysate","level":3,"score":0.7025019526481628},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.6993616819381714},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6942287683486938},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.5899595618247986},{"id":"https://openalex.org/C75280812","wikidata":"https://www.wikidata.org/wiki/Q190382","display_name":"Matrix-assisted laser desorption/ionization","level":4,"score":0.5884381532669067},{"id":"https://openalex.org/C202157981","wikidata":"https://www.wikidata.org/wiki/Q7251488","display_name":"Protein mass spectrometry","level":4,"score":0.5710339546203613},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.4805329442024231},{"id":"https://openalex.org/C162711632","wikidata":"https://www.wikidata.org/wiki/Q905514","display_name":"Desorption","level":3,"score":0.4771822690963745},{"id":"https://openalex.org/C16993767","wikidata":"https://www.wikidata.org/wiki/Q4674072","display_name":"Acid hydrolysis","level":3,"score":0.45831745862960815},{"id":"https://openalex.org/C2777436746","wikidata":"https://www.wikidata.org/wiki/Q2409","display_name":"Hydrochloric acid","level":2,"score":0.44745346903800964},{"id":"https://openalex.org/C27420735","wikidata":"https://www.wikidata.org/wiki/Q5968721","display_name":"Peptide mass fingerprinting","level":4,"score":0.44425395131111145},{"id":"https://openalex.org/C106487976","wikidata":"https://www.wikidata.org/wiki/Q685816","display_name":"Matrix (chemical analysis)","level":2,"score":0.4166163206100464},{"id":"https://openalex.org/C31827203","wikidata":"https://www.wikidata.org/wiki/Q874296","display_name":"Tandem mass spectrometry","level":3,"score":0.2866838276386261},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22036373615264893},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.15851560235023499},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.11777853965759277},{"id":"https://openalex.org/C150394285","wikidata":"https://www.wikidata.org/wiki/Q180254","display_name":"Adsorption","level":2,"score":0.0562574565410614},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006868","descriptor_name":"Hydrolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007834","descriptor_name":"Lasers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010449","descriptor_name":"Peptide Mapping","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/rcm.1290081220","doi":"https://doi.org/10.1002/rcm.1290081220","is_oa":false,"landing_page_url":"https://doi.org/10.1002/rcm.1290081220","pdf_url":null,"source":{"id":"https://openalex.org/S89689683","display_name":"Rapid Communications in Mass Spectrometry","issn_l":"0951-4198","issn":["0951-4198","1097-0231"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rapid Communications in Mass Spectrometry","raw_type":"journal-article"},{"id":"pmid:7696698","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7696698","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rapid communications in mass spectrometry : RCM","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":5,"referenced_works":["https://openalex.org/W127134555","https://openalex.org/W1921755785","https://openalex.org/W1972791227","https://openalex.org/W1974302575","https://openalex.org/W2119376397"],"related_works":["https://openalex.org/W2119868836","https://openalex.org/W2231455539","https://openalex.org/W2001958271","https://openalex.org/W4297453196","https://openalex.org/W2055122358","https://openalex.org/W7096985823","https://openalex.org/W3005066136","https://openalex.org/W2073082180","https://openalex.org/W2253103618","https://openalex.org/W1573840276"],"abstract_inverted_index":{"A":[0,95],"method":[1],"is":[2],"described":[3],"by":[4,25,69,164],"which":[5],"a":[6,109,142,146],"sequence-dependent":[7,143],"peptide":[8,147,179],"fingerprint":[9],"can":[10,66,160],"be":[11,67,138,161,169],"rapidly":[12],"obtained":[13,104],"upon":[14],"partial":[15],"hydrolysis":[16,85,124],"of":[17,91,155,176],"peptides":[18,34,177],"with":[19,37,152],"hydrochloric":[20],"acid":[21,73,107],"and":[22,126,149],"subsequent":[23],"analysis":[24],"matrix-assisted":[26],"laser":[27],"desorption/ionization":[28],"mass":[29,75],"spectrometry":[30],"(MALDI-MS).":[31],"When":[32],"synthetic":[33],"are":[35,49],"treated":[36],"3M":[38],"HCI":[39],"for":[40,145,172],"5":[41],"min":[42],"at":[43],"110":[44],"degrees":[45],"C,":[46],"amino":[47,72],"acids":[48],"released":[50],"in":[51,80],"turn":[52],"from":[53,61,88,99],"the":[54,59,62,71,78,83,89,92,100,158,174],"C-terminus":[55,101],"or,":[56],"depending":[57],"on":[58],"peptide,":[60,156],"N-terminus.":[63],"Sequence":[64],"information":[65],"deduced":[68],"identifying":[70],"whose":[74],"corresponds":[76],"to":[77,130,140],"difference":[79],"MW":[81,90],"between":[82],"major":[84],"products,":[86],"beginning":[87],"starting":[93,151],"peptide.":[94],"similar":[96],"pattern":[97],"exclusively":[98],"has":[102],"been":[103],"using":[105],"pentafluoropropionic":[106],"as":[108],"hydrolyzing":[110],"agent":[111],"(Tsugita":[112],"et":[113],"al.":[114],"Eur.":[115],"J.":[116],"Biochem.":[117],"206,":[118],"691":[119],"(1992)),":[120],"but":[121],"required":[122],"longer":[123],"time":[125],"more":[127],"handling":[128],"prior":[129],"analysis.":[131],"The":[132],"technique":[133],"we":[134],"have":[135],"developed":[136],"could":[137],"used":[139],"obtain":[141],"'fingerprint'":[144],"cheaply":[148],"rapidly,":[150],"picomole":[153],"amounts":[154],"because":[157],"hydrolysate":[159],"directly":[162],"analyzed":[163],"MALDI.":[165],"This":[166],"methodology":[167],"might":[168],"especially":[170],"useful":[171],"confirming":[173],"identity":[175],"during":[178],"mapping.":[180]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}