{"id":"https://openalex.org/W2099593237","doi":"https://doi.org/10.1083/jcb.135.6.1515","title":"Exclusion of CD45 inhibits activity of p56lck associated with glycolipid-enriched membrane domains.","display_name":"Exclusion of CD45 inhibits activity of p56lck associated with glycolipid-enriched membrane domains.","publication_year":1996,"publication_date":"1996-12-15","ids":{"openalex":"https://openalex.org/W2099593237","doi":"https://doi.org/10.1083/jcb.135.6.1515","mag":"2099593237","pmid":"https://pubmed.ncbi.nlm.nih.gov/8978819","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2133949"},"language":"en","primary_location":{"id":"doi:10.1083/jcb.135.6.1515","doi":"https://doi.org/10.1083/jcb.135.6.1515","is_oa":true,"landing_page_url":"https://doi.org/10.1083/jcb.135.6.1515","pdf_url":"https://rupress.org/jcb/article-pdf/135/6/1515/1268038/1515.pdf","source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://rupress.org/jcb/article-pdf/135/6/1515/1268038/1515.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5059663610","display_name":"William Rodgers","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"W Rodgers","raw_affiliation_strings":["Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112718152","display_name":"John K. Rose","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J K Rose","raw_affiliation_strings":["Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA","institution_ids":["https://openalex.org/I32971472"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I32971472"],"apc_list":null,"apc_paid":null,"fwci":4.9592,"has_fulltext":true,"cited_by_count":305,"citation_normalized_percentile":{"value":0.96377212,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"135","issue":"6","first_page":"1515","last_page":"1523"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.6298999786376953,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.6298999786376953,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.3037000000476837,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12104","display_name":"Protein Tyrosine Phosphatases","score":0.02810000069439411,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lck","display_name":"Lck","score":0.875},{"id":"https://openalex.org/keywords/cd45","display_name":"CD45","score":0.9380000233650208},{"id":"https://openalex.org/keywords/src-family-kinases","display_name":"Src family kinases","score":0.8370000123977661},{"id":"https://openalex.org/keywords/tyrosine-phosphorylation","display_name":"tyrosine phosphorylation","score":0.7929999828338623},{"id":"https://openalex.org/keywords/kinase-activity","display_name":"kinase activity","score":0.7760000228881836},{"id":"https://openalex.org/keywords/t-cell-signaling","display_name":"T cell signaling","score":0.6949999928474426},{"id":"https://openalex.org/keywords/membrane-domains","display_name":"membrane domains","score":0.42500001192092896},{"id":"https://openalex.org/keywords/jurkat-cells","display_name":"Jurkat cells","score":0.703000009059906},{"id":"https://openalex.org/keywords/protein-tyrosine-phosphatase","display_name":"protein tyrosine phosphatase","score":0.7360000014305115}],"concepts":[{"id":"https://openalex.org/C178666793","wikidata":"https://www.wikidata.org/wiki/Q422476","display_name":"Phosphatase","level":3,"score":0.6151210069656372},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6028276681900024},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.5968520045280457},{"id":"https://openalex.org/C2778198054","wikidata":"https://www.wikidata.org/wiki/Q15195154","display_name":"Hyperphosphorylation","level":3,"score":0.5815646648406982},{"id":"https://openalex.org/C85528070","wikidata":"https://www.wikidata.org/wiki/Q643602","display_name":"Protein tyrosine phosphatase","level":3,"score":0.5536394119262695},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.533499538898468},{"id":"https://openalex.org/C2777553839","wikidata":"https://www.wikidata.org/wiki/Q14330986","display_name":"Tyrosine phosphorylation","level":3,"score":0.5061618685722351},{"id":"https://openalex.org/C179464577","wikidata":"https://www.wikidata.org/wiki/Q1632589","display_name":"Jurkat cells","level":4,"score":0.5054912567138672},{"id":"https://openalex.org/C197153747","wikidata":"https://www.wikidata.org/wiki/Q24780316","display_name":"SH2 domain","level":4,"score":0.4941723644733429},{"id":"https://openalex.org/C108636557","wikidata":"https://www.wikidata.org/wiki/Q21109354","display_name":"Proto-oncogene tyrosine-protein kinase Src","level":3,"score":0.4863610863685608},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.4509229362010956},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4470507800579071},{"id":"https://openalex.org/C42362537","wikidata":"https://www.wikidata.org/wiki/Q87099740","display_name":"Tyrosine kinase","level":3,"score":0.4454272985458374},{"id":"https://openalex.org/C2776165026","wikidata":"https://www.wikidata.org/wiki/Q188017","display_name":"Tyrosine","level":2,"score":0.4352574646472931},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.42576494812965393},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.35319679975509644},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.2347184419631958},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.23438510298728943},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.0},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002458","descriptor_name":"Cell Fractionation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006017","descriptor_name":"Glycolipids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008563","descriptor_name":"Membrane Lipids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014443","descriptor_name":"Tyrosine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017493","descriptor_name":"Leukocyte Common Antigens","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D019061","descriptor_name":"src-Family Kinases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D019061","descriptor_name":"src-Family Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D019169","descriptor_name":"Jurkat Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019860","descriptor_name":"Lymphocyte Specific Protein Tyrosine Kinase p56(lck)","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1083/jcb.135.6.1515","doi":"https://doi.org/10.1083/jcb.135.6.1515","is_oa":true,"landing_page_url":"https://doi.org/10.1083/jcb.135.6.1515","pdf_url":"https://rupress.org/jcb/article-pdf/135/6/1515/1268038/1515.pdf","source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"journal-article"},{"id":"pmid:8978819","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8978819","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2133949","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2133949/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Cell Biol","raw_type":"Text"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.274.1624","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.274.1624","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/25/9e/J_Cell_Biol_1996_Dec_2_135(6)_1515-1523.tar.gz","raw_type":"text"}],"best_oa_location":{"id":"doi:10.1083/jcb.135.6.1515","doi":"https://doi.org/10.1083/jcb.135.6.1515","is_oa":true,"landing_page_url":"https://doi.org/10.1083/jcb.135.6.1515","pdf_url":"https://rupress.org/jcb/article-pdf/135/6/1515/1268038/1515.pdf","source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4043000042438507,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G5537212882","display_name":null,"funder_award_id":"AI-24345","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2099593237.pdf","grobid_xml":"https://content.openalex.org/works/W2099593237.grobid-xml"},"referenced_works_count":68,"referenced_works":["https://openalex.org/W1483782840","https://openalex.org/W1494595774","https://openalex.org/W1517168785","https://openalex.org/W1563035509","https://openalex.org/W1571171030","https://openalex.org/W1582379379","https://openalex.org/W1602215669","https://openalex.org/W1608138975","https://openalex.org/W1772739390","https://openalex.org/W1795202973","https://openalex.org/W1872676673","https://openalex.org/W1890859862","https://openalex.org/W1909995282","https://openalex.org/W1941725045","https://openalex.org/W1943505406","https://openalex.org/W1945795093","https://openalex.org/W1952552905","https://openalex.org/W1964774404","https://openalex.org/W1967234833","https://openalex.org/W1970830762","https://openalex.org/W1973598651","https://openalex.org/W1974928798","https://openalex.org/W1976010795","https://openalex.org/W1978086947","https://openalex.org/W1987359407","https://openalex.org/W1990693463","https://openalex.org/W1992692912","https://openalex.org/W1994772282","https://openalex.org/W2004793282","https://openalex.org/W2005411001","https://openalex.org/W2007640430","https://openalex.org/W2012681367","https://openalex.org/W2013267240","https://openalex.org/W2018289835","https://openalex.org/W2023965621","https://openalex.org/W2024753337","https://openalex.org/W2028669354","https://openalex.org/W2029298164","https://openalex.org/W2034152669","https://openalex.org/W2035562653","https://openalex.org/W2040465119","https://openalex.org/W2042824379","https://openalex.org/W2047907583","https://openalex.org/W2050465028","https://openalex.org/W2064754325","https://openalex.org/W2068908631","https://openalex.org/W2092732198","https://openalex.org/W2099204510","https://openalex.org/W2100837269","https://openalex.org/W2104670055","https://openalex.org/W2109202813","https://openalex.org/W2111813696","https://openalex.org/W2114844324","https://openalex.org/W2115051410","https://openalex.org/W2118510702","https://openalex.org/W2119737153","https://openalex.org/W2123030732","https://openalex.org/W2129030094","https://openalex.org/W2133388060","https://openalex.org/W2144760773","https://openalex.org/W2146611925","https://openalex.org/W2156320225","https://openalex.org/W2163790379","https://openalex.org/W2163995402","https://openalex.org/W2169976308","https://openalex.org/W2181131337","https://openalex.org/W2419476448","https://openalex.org/W2471808755"],"related_works":["https://openalex.org/W7100396030","https://openalex.org/W2012681367","https://openalex.org/W2384283309","https://openalex.org/W2138128599","https://openalex.org/W2467593455","https://openalex.org/W2109202813","https://openalex.org/W2093365663","https://openalex.org/W2117707012","https://openalex.org/W1548984399","https://openalex.org/W2159431877"],"abstract_inverted_index":{"p56lck":[0],"(Lck)":[1],"is":[2,10,18,30,39,179],"a":[3,20,33,88,159,197],"lymphoid-specific":[4],"Src":[5],"family":[6],"tyrosine":[7,130,171],"kinase":[8,90,109,174],"that":[9,38,104,127,154,167,201],"critical":[11],"for":[12],"T-cell":[13],"development":[14],"and":[15,23,56,84,100,107,149,166,172],"activation.":[16],"Lck":[17,29,51,62,72,113,146,178,188],"also":[19],"membrane":[21,35,161],"protein,":[22],"approximately":[24],"half":[25],"of":[26,59,111,118,145,170,176,183,199],"the":[27,49,54,74,95,105,119,128,136,155,168,191],"membrane-associated":[28,50],"associated":[31,189],"with":[32,68,87,190],"glycolipid-enriched":[34],"(GEM)":[36],"fraction":[37,65,76,157],"resistant":[40],"to":[41,70,79,94,116,181],"solubilization":[42],"by":[43],"Triton":[44],"X-100":[45],"(TX-100).":[46],"To":[47],"compare":[48],"present":[52,163],"in":[53,73,147,164],"GEM":[55,75,137,148,156,192],"TX-100-soluble":[57,96,150],"fractions":[58],"Jurkat":[60],"cells,":[61],"from":[63,135,185],"each":[64],"was":[66,77,114,133],"immunoblotted":[67],"antibody":[69],"phosphotyrosine.":[71],"found":[78],"be":[80,203],"hyperphosphorylated":[81],"on":[82],"tyrosine,":[83],"this":[85],"correlated":[86],"lower":[89,108,173],"specific":[91,160],"activity":[92,110,175],"relative":[93],"Lck.":[97],"Peptide":[98],"mapping":[99],"phosphatase":[101,131],"diagests":[102],"showed":[103,142],"hyperphosphorylation":[106,169],"GEM-associated":[112,177],"due":[115,180],"phosphorylation":[117,144],"regulatory":[120],"COOH-terminal":[121],"Tyr505.":[122],"In":[123],"addition,":[124],"we":[125],"determined":[126],"membrane-bound":[129],"CD45":[132,141,184],"absent":[134],"fraction.":[138],"Cells":[139],"lacking":[140],"identical":[143],"membranes.":[151],"We":[152],"propose":[153],"represents":[158],"domain":[162],"T-cells,":[165],"exclusion":[182],"these":[186],"domains.":[187],"domains":[193],"may":[194],"therefore":[195],"consitute":[196],"reservoir":[198],"enzyme":[200],"can":[202],"readily":[204],"activated.":[205]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":6}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}