{"id":"https://openalex.org/W209958151","doi":"https://doi.org/10.21236/ad0857265","title":"Identification of Clones of Mammalian Cells by Enzymatic Activity and Isoenzyme Distribution Patterns","display_name":"Identification of Clones of Mammalian Cells by Enzymatic Activity and Isoenzyme Distribution Patterns","publication_year":1969,"publication_date":"1969-07-01","ids":{"openalex":"https://openalex.org/W209958151","doi":"https://doi.org/10.21236/ad0857265","mag":"209958151"},"language":"en","primary_location":{"id":"doi:10.21236/ad0857265","doi":"https://doi.org/10.21236/ad0857265","is_oa":false,"landing_page_url":"http://doi.org/10.21236/ad0857265","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"US Dept of the Army","raw_type":"report"},"volume":null,"type":"report","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108573832","display_name":"Donald C. Fish","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Donald C. Fish","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000702620","display_name":"James P. Dobbs","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"James P. Dobbs","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5101514173","display_name":"Richard C. Carter","orcid":"https://orcid.org/0000-0003-1291-4187","observed_orcids":["https://orcid.org/0000-0003-1291-4187"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Richard C. Carter","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T14135","display_name":"Cancer and biochemical research","score":0.6845999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T14135","display_name":"Cancer and biochemical research","score":0.6845999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.14949999749660492,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.025599999353289604,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/isoenzyme-distribution","display_name":"isoenzyme distribution","score":0.7310000061988831},{"id":"https://openalex.org/keywords/zymography","display_name":"zymography","score":0.9760000109672546},{"id":"https://openalex.org/keywords/enzyme-activity","display_name":"enzyme activity","score":0.753000020980835},{"id":"https://openalex.org/keywords/subclones","display_name":"subclones","score":0.6460000276565552},{"id":"https://openalex.org/keywords/esterase","display_name":"esterase","score":0.5249999761581421},{"id":"https://openalex.org/keywords/suspension-culture","display_name":"suspension culture","score":0.902999997138977},{"id":"https://openalex.org/keywords/monolayer-culture","display_name":"monolayer culture","score":0.9769999980926514},{"id":"https://openalex.org/keywords/cell-cycle","display_name":"cell cycle","score":0.5630000233650208},{"id":"https://openalex.org/keywords/cell-identification","display_name":"cell identification","score":0.5649999976158142}],"concepts":[{"id":"https://openalex.org/C119795356","wikidata":"https://www.wikidata.org/wiki/Q407801","display_name":"Isozyme","level":3,"score":0.7247833013534546},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.6245875954627991},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5754752159118652},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5025300979614258},{"id":"https://openalex.org/C110121322","wikidata":"https://www.wikidata.org/wiki/Q865811","display_name":"Distribution (mathematics)","level":2,"score":0.4537494480609894},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4282488226890564},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.32325494289398193},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.09491533041000366},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.05693444609642029},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.21236/ad0857265","doi":"https://doi.org/10.21236/ad0857265","is_oa":false,"landing_page_url":"http://doi.org/10.21236/ad0857265","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"US Dept of the Army","raw_type":"report"},{"id":"pmh:AD0857265","doi":null,"is_oa":false,"landing_page_url":"http://oai.dtic.mil/oai/oai?&verb=getRecord&metadataPrefix=html&identifier=AD0857265","pdf_url":null,"source":{"id":"https://openalex.org/S4406923043","display_name":"Defense Technical Information Center (DTIC)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"DTIC AND NTIS","raw_type":"Text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2023767820","https://openalex.org/W265851304","https://openalex.org/W2016591375","https://openalex.org/W1993411472","https://openalex.org/W1997027633","https://openalex.org/W2015353382","https://openalex.org/W2333376663","https://openalex.org/W2411556040","https://openalex.org/W1994993624","https://openalex.org/W2398588001"],"abstract_inverted_index":{"Isoenzyme":[0],"distribution":[1],"patterns":[2],"(zymograms)":[3],"and":[4,12,24,64,79,92,109,113],"enzymic-specific":[5,119],"activities":[6,120],"were":[7,32,67,124,129],"determined":[8],"for":[9,40,52],"both":[10],"suspension":[11,61],"monolayer":[13,35],"cultures":[14,36,45,123,128],"of":[15,37,46,89,98],"the":[16,70,90,93,99,127,132],"LM":[17],"cell":[18],"line,":[19],"its":[20],"clone":[21],"(Cl":[22],"l),":[23],"four":[25],"sub-clones":[26,95],"from":[27,34,73,82,87,121],"this":[28,102],"clone.":[29,75],"Identical":[30],"zymograms":[31,51,72,81],"obtained":[33],"all":[38,41,47,122],"clones":[39,48,115],"enzymes":[42],"tested.":[43],"Suspension":[44],"yielded":[49],"identical":[50,125],"six":[53],"hydroxy":[54],"acid":[55],"dehydrogenases.":[56],"However,":[57],"when":[58,126],"grown":[59],"in":[60,69,116,135],"culture,":[62],"reproducible":[63],"characteristic":[65],"differences":[66],"found":[68],"esterase":[71,80],"each":[74],"The":[76,118],"growth":[77,137],"pattern":[78],"sub-clone":[83],"1-9":[84],"varied":[85],"more":[86],"those":[88],"parent":[91],"other":[94],"than":[96],"any":[97],"others.":[100],"Thus,":[101],"procedure":[103],"can":[104],"be":[105],"used":[106],"to":[107],"identify":[108],"characterize":[110],"tissue":[111],"cells":[112],"their":[114,136],"culture.":[117],"sampled":[130],"at":[131],"same":[133],"stage":[134],"cycle.":[138]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}