{"id":"https://openalex.org/W2099454548","doi":"https://doi.org/10.1104/pp.15.00638","title":"A Universal Positive-Negative Selection System for Gene Targeting in Plants Combining an Antibiotic Resistance Gene and Its Antisense RNA","display_name":"A Universal Positive-Negative Selection System for Gene Targeting in Plants Combining an Antibiotic Resistance Gene and Its Antisense RNA","publication_year":2015,"publication_date":"2015-07-04","ids":{"openalex":"https://openalex.org/W2099454548","doi":"https://doi.org/10.1104/pp.15.00638","mag":"2099454548","pmid":"https://pubmed.ncbi.nlm.nih.gov/26143254","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4577407"},"language":"en","primary_location":{"id":"doi:10.1104/pp.15.00638","doi":"https://doi.org/10.1104/pp.15.00638","is_oa":false,"landing_page_url":"https://doi.org/10.1104/pp.15.00638","pdf_url":null,"source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC4577407/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003124422","display_name":"Ayako Nishizawa\u2010Yokoi","orcid":"https://orcid.org/0000-0003-2781-1359","observed_orcids":["https://orcid.org/0000-0003-2781-1359"]},"institutions":[{"id":"https://openalex.org/I1283532511","display_name":"Kihara Institute for Biological Research","ror":"https://ror.org/007hwje16","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1283532511"]},{"id":"https://openalex.org/I3018761593","display_name":"Institute of Agrobiological Sciences","ror":"https://ror.org/01786mp71","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I3018761593"]},{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]},{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Ayako Nishizawa-Yokoi","raw_affiliation_strings":["Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305\u20138602, Japan (A.N.-Y., S.N., K.O., H.S., S.T.); Center for Collaboration among Agriculture, Industry and Commerce, University of Tokushima, Tokushima 770\u20138503, Japan (K.O.); and Kihara Institute for Biological Research, Yokohama City University, Yokohama 244\u20130813, Japan (S.T.)"],"raw_orcid":"https://orcid.org/0000-0003-2781-1359","affiliations":[{"raw_affiliation_string":"Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305\u20138602, Japan (A.N.-Y., S.N., K.O., H.S., S.T.); Center for Collaboration among Agriculture, Industry and Commerce, University of Tokushima, Tokushima 770\u20138503, Japan (K.O.); and Kihara Institute for Biological Research, Yokohama City University, Yokohama 244\u20130813, Japan (S.T.)","institution_ids":["https://openalex.org/I1283532511","https://openalex.org/I3018761593","https://openalex.org/I89630735","https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029803018","display_name":"Satoko Nonaka","orcid":"https://orcid.org/0000-0001-9192-7968","observed_orcids":["https://orcid.org/0000-0001-9192-7968"]},"institutions":[{"id":"https://openalex.org/I1283532511","display_name":"Kihara Institute for Biological Research","ror":"https://ror.org/007hwje16","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1283532511"]},{"id":"https://openalex.org/I3018761593","display_name":"Institute of Agrobiological Sciences","ror":"https://ror.org/01786mp71","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I3018761593"]},{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]},{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Satoko Nonaka","raw_affiliation_strings":["Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305\u20138602, Japan (A.N.-Y., S.N., K.O., H.S., S.T.); Center for Collaboration among Agriculture, Industry and Commerce, University of Tokushima, Tokushima 770\u20138503, Japan (K.O.); and Kihara Institute for Biological Research, Yokohama City University, Yokohama 244\u20130813, Japan (S.T.)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305\u20138602, Japan (A.N.-Y., S.N., K.O., H.S., S.T.); Center for Collaboration among Agriculture, Industry and Commerce, University of Tokushima, Tokushima 770\u20138503, Japan (K.O.); and Kihara Institute for Biological Research, Yokohama City University, Yokohama 244\u20130813, Japan (S.T.)","institution_ids":["https://openalex.org/I1283532511","https://openalex.org/I3018761593","https://openalex.org/I89630735","https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054315487","display_name":"Keishi Osakabe","orcid":"https://orcid.org/0000-0002-6227-6913","observed_orcids":["https://orcid.org/0000-0002-6227-6913"]},"institutions":[{"id":"https://openalex.org/I1283532511","display_name":"Kihara Institute for Biological Research","ror":"https://ror.org/007hwje16","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1283532511"]},{"id":"https://openalex.org/I3018761593","display_name":"Institute of Agrobiological Sciences","ror":"https://ror.org/01786mp71","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I3018761593"]},{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]},{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Keishi Osakabe","raw_affiliation_strings":["Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305\u20138602, Japan (A.N.-Y., S.N., K.O., H.S., S.T.); Center for Collaboration among Agriculture, Industry and Commerce, University of Tokushima, Tokushima 770\u20138503, Japan (K.O.); and Kihara Institute for Biological Research, Yokohama City University, Yokohama 244\u20130813, Japan (S.T.)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305\u20138602, Japan (A.N.-Y., S.N., K.O., H.S., S.T.); Center for Collaboration among Agriculture, Industry and Commerce, University of Tokushima, Tokushima 770\u20138503, Japan (K.O.); and Kihara Institute for Biological Research, Yokohama City University, Yokohama 244\u20130813, Japan (S.T.)","institution_ids":["https://openalex.org/I1283532511","https://openalex.org/I3018761593","https://openalex.org/I89630735","https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071782068","display_name":"Hiroaki Saika","orcid":"https://orcid.org/0000-0002-0716-2518","observed_orcids":["https://orcid.org/0000-0002-0716-2518"]},"institutions":[{"id":"https://openalex.org/I1283532511","display_name":"Kihara Institute for Biological Research","ror":"https://ror.org/007hwje16","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1283532511"]},{"id":"https://openalex.org/I3018761593","display_name":"Institute of Agrobiological Sciences","ror":"https://ror.org/01786mp71","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I3018761593"]},{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]},{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroaki Saika","raw_affiliation_strings":["Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305\u20138602, Japan (A.N.-Y., S.N., K.O., H.S., S.T.); Center for Collaboration among Agriculture, Industry and Commerce, University of Tokushima, Tokushima 770\u20138503, Japan (K.O.); and Kihara Institute for Biological Research, Yokohama City University, Yokohama 244\u20130813, Japan (S.T.)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305\u20138602, Japan (A.N.-Y., S.N., K.O., H.S., S.T.); Center for Collaboration among Agriculture, Industry and Commerce, University of Tokushima, Tokushima 770\u20138503, Japan (K.O.); and Kihara Institute for Biological Research, Yokohama City University, Yokohama 244\u20130813, Japan (S.T.)","institution_ids":["https://openalex.org/I1283532511","https://openalex.org/I3018761593","https://openalex.org/I89630735","https://openalex.org/I922474255"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5002001500","display_name":"Seiichi Toki","orcid":"https://orcid.org/0000-0003-0066-6446","observed_orcids":["https://orcid.org/0000-0003-0066-6446"]},"institutions":[{"id":"https://openalex.org/I1283532511","display_name":"Kihara Institute for Biological Research","ror":"https://ror.org/007hwje16","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1283532511"]},{"id":"https://openalex.org/I3018761593","display_name":"Institute of Agrobiological Sciences","ror":"https://ror.org/01786mp71","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1323638106","https://openalex.org/I3018761593"]},{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]},{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Seiichi Toki","raw_affiliation_strings":["Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305\u20138602, Japan (A.N.-Y., S.N., K.O., H.S., S.T.); Center for Collaboration among Agriculture, Industry and Commerce, University of Tokushima, Tokushima 770\u20138503, Japan (K.O.); and Kihara Institute for Biological Research, Yokohama City University, Yokohama 244\u20130813, Japan (S.T.)"],"raw_orcid":"https://orcid.org/0000-0003-0066-6446","affiliations":[{"raw_affiliation_string":"Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305\u20138602, Japan (A.N.-Y., S.N., K.O., H.S., S.T.); Center for Collaboration among Agriculture, Industry and Commerce, University of Tokushima, Tokushima 770\u20138503, Japan (K.O.); and Kihara Institute for Biological Research, Yokohama City University, Yokohama 244\u20130813, Japan (S.T.)","institution_ids":["https://openalex.org/I1283532511","https://openalex.org/I3018761593","https://openalex.org/I89630735","https://openalex.org/I922474255"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3851,"currency":"USD","value_usd":3851},"apc_paid":null,"fwci":1.2705,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.77547867,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"169","issue":"1","first_page":"362","last_page":"370"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.6050000190734863,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.6050000190734863,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.3337000012397766,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.0284000001847744,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gene-targeting","display_name":"gene targeting","score":0.9480000138282776},{"id":"https://openalex.org/keywords/positive-and-negative-selection","display_name":"positive and negative selection","score":0.9070000052452087},{"id":"https://openalex.org/keywords/neomycin-phosphotransferase-ii","display_name":"neomycin phosphotransferase II","score":0.9290000200271606},{"id":"https://openalex.org/keywords/hygromycin-resistance","display_name":"hygromycin resistance","score":0.8169999718666077},{"id":"https://openalex.org/keywords/transgenic-callus","display_name":"transgenic callus","score":0.5149999856948853},{"id":"https://openalex.org/keywords/rice","display_name":"rice","score":0.625},{"id":"https://openalex.org/keywords/waxy-gene","display_name":"waxy gene","score":0.7749999761581421},{"id":"https://openalex.org/keywords/knockout","display_name":"knockout","score":0.4050000011920929},{"id":"https://openalex.org/keywords/plant-genome-engineering","display_name":"plant genome engineering","score":0.5669999718666077}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.758151650428772},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6840754747390747},{"id":"https://openalex.org/C7386963","wikidata":"https://www.wikidata.org/wiki/Q3954859","display_name":"Negative selection","level":4,"score":0.5886032581329346},{"id":"https://openalex.org/C202416437","wikidata":"https://www.wikidata.org/wiki/Q423832","display_name":"Antisense RNA","level":4,"score":0.543976366519928},{"id":"https://openalex.org/C143141573","wikidata":"https://www.wikidata.org/wiki/Q7450971","display_name":"Sense (electronics)","level":2,"score":0.5028557181358337},{"id":"https://openalex.org/C177955015","wikidata":"https://www.wikidata.org/wiki/Q5532936","display_name":"Genetically modified rice","level":5,"score":0.4766962230205536},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.4663390815258026},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.4473068118095398},{"id":"https://openalex.org/C2778543611","wikidata":"https://www.wikidata.org/wiki/Q161426","display_name":"Oryza sativa","level":3,"score":0.4327704906463623},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4222198724746704},{"id":"https://openalex.org/C81917197","wikidata":"https://www.wikidata.org/wiki/Q628760","display_name":"Selection (genetic algorithm)","level":2,"score":0.41372743248939514},{"id":"https://openalex.org/C122172344","wikidata":"https://www.wikidata.org/wiki/Q1503477","display_name":"Genetically modified crops","level":4,"score":0.37706321477890015},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.29068523645401},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0606628954410553},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.0},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000483","descriptor_name":"Alleles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004352","descriptor_name":"Drug Resistance, Microbial","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D004352","descriptor_name":"Drug Resistance, Microbial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005839","descriptor_name":"Gentamicins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012275","descriptor_name":"Oryza","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D015139","descriptor_name":"Blotting, Southern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016372","descriptor_name":"RNA, Antisense","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017343","descriptor_name":"Genes, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018390","descriptor_name":"Gene Targeting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020090","descriptor_name":"Chromosome Segregation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055786","descriptor_name":"Gene Knockout Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056426","descriptor_name":"Genetic Loci","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1104/pp.15.00638","doi":"https://doi.org/10.1104/pp.15.00638","is_oa":false,"landing_page_url":"https://doi.org/10.1104/pp.15.00638","pdf_url":null,"source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},{"id":"pmid:26143254","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26143254","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant physiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4577407","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC4577407/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:4577407","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC4577407/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiol","raw_type":"Text"},"sustainable_development_goals":[{"score":0.8409000039100647,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":42,"referenced_works":["https://openalex.org/W25811119","https://openalex.org/W139193563","https://openalex.org/W1498463643","https://openalex.org/W1531079516","https://openalex.org/W1570739956","https://openalex.org/W1958388479","https://openalex.org/W1967151739","https://openalex.org/W1976544087","https://openalex.org/W1978207956","https://openalex.org/W1983568123","https://openalex.org/W1986703291","https://openalex.org/W1987402198","https://openalex.org/W1992791030","https://openalex.org/W1993881251","https://openalex.org/W1996756493","https://openalex.org/W1998503989","https://openalex.org/W2005927673","https://openalex.org/W2006123853","https://openalex.org/W2012079727","https://openalex.org/W2015015564","https://openalex.org/W2016845439","https://openalex.org/W2024556021","https://openalex.org/W2036679599","https://openalex.org/W2045458356","https://openalex.org/W2050024650","https://openalex.org/W2054302101","https://openalex.org/W2077489356","https://openalex.org/W2080191976","https://openalex.org/W2089988001","https://openalex.org/W2097600027","https://openalex.org/W2101179888","https://openalex.org/W2111210729","https://openalex.org/W2114071685","https://openalex.org/W2118377520","https://openalex.org/W2130084698","https://openalex.org/W2139671415","https://openalex.org/W2159388002","https://openalex.org/W2161322658","https://openalex.org/W2168581220","https://openalex.org/W2189430040","https://openalex.org/W2289813125","https://openalex.org/W2420411720"],"related_works":["https://openalex.org/W1967151739","https://openalex.org/W7096507650","https://openalex.org/W1996756493","https://openalex.org/W1586477929","https://openalex.org/W2114071685","https://openalex.org/W7097544888","https://openalex.org/W4249983598","https://openalex.org/W1980911839","https://openalex.org/W2018128328","https://openalex.org/W2092484925"],"abstract_inverted_index":{"Gene":[0],"targeting":[1],"(GT)":[2],"is":[3,39,159],"a":[4,18,58,68,72,126,136,181],"useful":[5],"technology":[6],"for":[7,140,185],"accurate":[8],"genome":[9],"engineering":[10],"in":[11,52,64,191],"plants.":[12],"A":[13,29,167],"reproducible":[14],"approach":[15,170],"based":[16],"on":[17],"positive-negative":[19,61],"selection":[20,36,46,69,128,138,155,183],"system":[21,47,63,70,158,184],"using":[22,71],"hygromycin":[23],"resistance":[24],"and":[25,34,78,89,103,106,130,148],"the":[26,94,99,111,121,131,143,186],"diphtheria":[27,165],"toxin":[28,166],"subunit":[30],"gene":[31,124],"as":[32,125,135,180],"positive":[33,127],"negative":[35,137,154],"markers,":[37],"respectively,":[38],"now":[40],"available.":[41],"However,":[42],"to":[43,110,119],"date,":[44],"this":[45,157],"has":[48],"been":[49],"applied":[50,179],"exclusively":[51],"rice":[53,145],"(Oryza":[54],"sativa).":[55],"To":[56],"establish":[57],"universally":[59],"applicable":[60],"GT":[62,189],"plants,":[65],"we":[66,116],"designed":[67],"combination":[73],"of":[74,86,96,98,142,188],"neomycin":[75],"phosphotransferaseII":[76],"(nptII)":[77],"an":[79],"antisense":[80,90,132],"nptII":[81,91,101,123,133],"construct.":[82],"The":[83,169],"concomitant":[84],"transcription":[85],"both":[87],"sense":[88,100,122],"suppresses":[92],"significantly":[93],"level":[95],"expression":[97],"gene,":[102],"transgenic":[104],"calli":[105],"plants":[107],"become":[108],"sensitive":[109],"antibiotic":[112],"geneticin.":[113],"In":[114],"addition,":[115],"were":[117],"able":[118],"utilize":[120],"marker":[129,139],"construct":[134],"knockout":[141],"endogenous":[144],"genes":[146],"Waxy":[147],"33-kD":[149],"globulin":[150],"through":[151],"GT,":[152],"although":[153],"with":[156,164,173],"relatively":[160],"less":[161],"efficient":[162],"compared":[163],"subunit.":[168],"developed":[171],"here,":[172],"some":[174],"additional":[175],"improvements,":[176],"could":[177],"be":[178],"universal":[182],"enrichment":[187],"cells":[190],"several":[192],"plant":[193],"species.":[194]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":7}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}