{"id":"https://openalex.org/W2098932913","doi":"https://doi.org/10.1095/biolreprod48.5.1047","title":"Localization of a Spermatid-Specific Histone 2B Protein in Mouse Spermiogenic Cells1","display_name":"Localization of a Spermatid-Specific Histone 2B Protein in Mouse Spermiogenic Cells1","publication_year":1993,"publication_date":"1993-05-01","ids":{"openalex":"https://openalex.org/W2098932913","doi":"https://doi.org/10.1095/biolreprod48.5.1047","mag":"2098932913","pmid":"https://pubmed.ncbi.nlm.nih.gov/8481469"},"language":"en","primary_location":{"id":"doi:10.1095/biolreprod48.5.1047","doi":"https://doi.org/10.1095/biolreprod48.5.1047","is_oa":false,"landing_page_url":"https://doi.org/10.1095/biolreprod48.5.1047","pdf_url":null,"source":{"id":"https://openalex.org/S130598054","display_name":"Biology of Reproduction","issn_l":"0006-3363","issn":["0006-3363","1529-7268"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biology of Reproduction","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103355093","display_name":"Stuart B. Moss","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Stuart B. Moss","raw_affiliation_strings":["Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia 19104","Division of Reproductive Biology, Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia 19104","institution_ids":["https://openalex.org/I79576946"]},{"raw_affiliation_string":"Division of Reproductive Biology, Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5052253390","display_name":"Joanne M. Orth","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joanne M. Orth","raw_affiliation_strings":["Department of Anatomy and Cell Biology, Temple University School of Medicine Philadelphia, Pennsylvania 19140","Department of Anatomy & Cell Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy and Cell Biology, Temple University School of Medicine Philadelphia, Pennsylvania 19140","institution_ids":["https://openalex.org/I84392919"]},{"raw_affiliation_string":"Department of Anatomy & Cell Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140","institution_ids":["https://openalex.org/I84392919"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5103355093"],"corresponding_institution_ids":["https://openalex.org/I79576946"],"apc_list":{"value":4605,"currency":"USD","value_usd":4605},"apc_paid":null,"fwci":2.4935,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.87888413,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"48","issue":"5","first_page":"1047","last_page":"1056"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.0038999998942017555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11505","display_name":"Reproductive biology and impacts on aquatic species","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/round-spermatids","display_name":"round spermatids","score":0.7940000295639038},{"id":"https://openalex.org/keywords/spermiogenesis","display_name":"spermiogenesis","score":0.9769999980926514},{"id":"https://openalex.org/keywords/histone-replacement","display_name":"histone replacement","score":0.6069999933242798},{"id":"https://openalex.org/keywords/protamine","display_name":"protamine","score":0.8460000157356262},{"id":"https://openalex.org/keywords/chromatin-condensation","display_name":"chromatin condensation","score":0.925000011920929},{"id":"https://openalex.org/keywords/nuclear-envelope","display_name":"nuclear envelope","score":0.8799999952316284},{"id":"https://openalex.org/keywords/nucleolus","display_name":"nucleolus","score":0.8489999771118164},{"id":"https://openalex.org/keywords/sperm-chromatin","display_name":"sperm chromatin","score":0.5289999842643738},{"id":"https://openalex.org/keywords/mouse-testis","display_name":"mouse testis","score":0.7519999742507935}],"concepts":[{"id":"https://openalex.org/C2777394357","wikidata":"https://www.wikidata.org/wiki/Q1494188","display_name":"Spermatid","level":3,"score":0.9608800411224365},{"id":"https://openalex.org/C2778619154","wikidata":"https://www.wikidata.org/wiki/Q3493046","display_name":"Spermiogenesis","level":3,"score":0.8977057337760925},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7811565399169922},{"id":"https://openalex.org/C83640560","wikidata":"https://www.wikidata.org/wiki/Q180951","display_name":"Chromatin","level":3,"score":0.7544870376586914},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6612555980682373},{"id":"https://openalex.org/C64927066","wikidata":"https://www.wikidata.org/wiki/Q36293","display_name":"Histone","level":3,"score":0.5674858093261719},{"id":"https://openalex.org/C45413362","wikidata":"https://www.wikidata.org/wiki/Q6013254","display_name":"Prophase","level":4,"score":0.5545051097869873},{"id":"https://openalex.org/C2780723820","wikidata":"https://www.wikidata.org/wiki/Q1934178","display_name":"Nucleus","level":2,"score":0.5204256176948547},{"id":"https://openalex.org/C29512474","wikidata":"https://www.wikidata.org/wiki/Q16860021","display_name":"Nuclear protein","level":4,"score":0.5090681910514832},{"id":"https://openalex.org/C183873130","wikidata":"https://www.wikidata.org/wiki/Q30869","display_name":"Nucleolus","level":3,"score":0.46196693181991577},{"id":"https://openalex.org/C2780114586","wikidata":"https://www.wikidata.org/wiki/Q40260","display_name":"Cell nucleus","level":3,"score":0.456760436296463},{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.37527328729629517},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3689424395561218},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.24329552054405212},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.08547049760818481},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.059879809617996216},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.0},{"id":"https://openalex.org/C148196450","wikidata":"https://www.wikidata.org/wiki/Q21100485","display_name":"Meiosis","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006657","descriptor_name":"Histones","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D006657","descriptor_name":"Histones","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D006657","descriptor_name":"Histones","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D007124","descriptor_name":"Immunoenzyme Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009687","descriptor_name":"Nuclear Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D009687","descriptor_name":"Nuclear Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013087","descriptor_name":"Spermatids","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D013087","descriptor_name":"Spermatids","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013090","descriptor_name":"Spermatocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013090","descriptor_name":"Spermatocytes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013091","descriptor_name":"Spermatogenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1095/biolreprod48.5.1047","doi":"https://doi.org/10.1095/biolreprod48.5.1047","is_oa":false,"landing_page_url":"https://doi.org/10.1095/biolreprod48.5.1047","pdf_url":null,"source":{"id":"https://openalex.org/S130598054","display_name":"Biology of Reproduction","issn_l":"0006-3363","issn":["0006-3363","1529-7268"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biology of Reproduction","raw_type":"journal-article"},{"id":"pmid:8481469","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8481469","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biology of reproduction","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1146496935","display_name":"MAMMALIAN SPERMATOGENESIS--HISTONES/DNA REORGANIZATION","funder_award_id":"5r01hd025524-04","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G2008155340","display_name":null,"funder_award_id":"HD25524","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"},{"id":"https://openalex.org/G5418711647","display_name":null,"funder_award_id":"HD15563","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"},{"id":"https://openalex.org/G965371001","display_name":"FERTILITY AND SERTOLI CELL DEVELOPMENT","funder_award_id":"5r01hd015563-22","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337611","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W644015436","https://openalex.org/W1521702495","https://openalex.org/W1537218255","https://openalex.org/W1542177054","https://openalex.org/W1604083601","https://openalex.org/W1850278213","https://openalex.org/W1975908401","https://openalex.org/W1978270484","https://openalex.org/W1986143650","https://openalex.org/W1993305464","https://openalex.org/W1999283186","https://openalex.org/W2008427146","https://openalex.org/W2019296561","https://openalex.org/W2022327781","https://openalex.org/W2028895755","https://openalex.org/W2029998567","https://openalex.org/W2030396609","https://openalex.org/W2051627482","https://openalex.org/W2053508758","https://openalex.org/W2063085696","https://openalex.org/W2072177313","https://openalex.org/W2074080513","https://openalex.org/W2074168609","https://openalex.org/W2094410401","https://openalex.org/W2100837269","https://openalex.org/W2131018415","https://openalex.org/W2403635918","https://openalex.org/W2404350895"],"related_works":["https://openalex.org/W7097147367","https://openalex.org/W2052019435","https://openalex.org/W2022327781","https://openalex.org/W1980080473","https://openalex.org/W1991494206","https://openalex.org/W2100703255","https://openalex.org/W389083620","https://openalex.org/W1973538966","https://openalex.org/W1482657485","https://openalex.org/W4296201815"],"abstract_inverted_index":{"Mammalian":[0],"spermiogenesis":[1],"is":[2,43,116,140,191],"characterized":[3],"by":[4,19],"chromatin":[5,71,184],"condensation":[6,72],"and":[7,15,45,109,193],"replacement":[8],"of":[9,13,53,70,96,100,120,164,200],"the":[10,22,58,64,67,79,93,97,107,124,134,148,159,162,168,172,183],"histones":[11],"typical":[12],"somatic":[14],"earlier":[16],"spermatogenic":[17,201],"cells":[18],"protamines":[20],"in":[21,39,47,78,118,143,147,178],"nucleus.":[23],"However,":[24],"a":[25,36,88,197],"spermatid-specific":[26],"H2b":[27],"histone":[28],"(ssH2b)":[29],"that":[30,57,114,138,189],"has":[31],"an":[32,82],"unusual":[33],"carboxyl-terminus":[34,95],"containing":[35],"region":[37,55],"rich":[38],"hydrophobic":[40],"amino":[41],"acids":[42],"transcribed":[44],"translated":[46],"mouse":[48],"round":[49,121,149],"spermatids.":[50,122],"The":[51],"hydrophobicity":[52],"this":[54],"suggested":[56],"protein":[59,125,174],"may":[60],"be":[61,130,155],"localized":[62],"at":[63,105],"nuclear":[65,135],"envelope,":[66,136],"initial":[68],"site":[69],"during":[73,196],"spermiogenesis.":[74],"To":[75],"identify":[76],"ssH2b":[77,115,173],"spermatid":[80],"nucleus,":[81],"antiserum":[83],"(anti-ss-H2b127-138)":[84],"was":[85,126,175,185],"generated":[86],"against":[87],"synthetic":[89],"peptide":[90],"corresponding":[91],"to":[92,129,154],"unique":[94],"protein.":[98],"Immunocytochemistry":[99],"fixed,":[101],"frozen":[102],"testicular":[103],"sections":[104],"both":[106],"light":[108],"electron":[110],"microscopic":[111],"levels":[112],"indicated":[113],"present":[117,192],"nuclei":[119],"Moreover,":[123],"not":[127,141,176],"found":[128,177],"preferentially":[131],"associated":[132],"with":[133,161],"indicating":[137],"it":[139,152,190],"involved":[142],"chromatin-nuclear":[144],"envelope":[145],"interaction":[146],"spermatid.":[150],"Rather,":[151],"appeared":[153],"distributed":[156],"uniformly":[157],"throughout":[158],"nucleus":[160],"exception":[163],"its":[165],"exclusion":[166],"from":[167],"nucleolus.":[169],"In":[170],"addition,":[171],"mature":[179],"sperm":[180],"even":[181],"when":[182],"partially":[186],"decondensed,":[187],"suggesting":[188],"functions":[194],"only":[195],"restricted":[198],"period":[199],"development.":[202]},"counts_by_year":[{"year":2019,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}