{"id":"https://openalex.org/W2098505754","doi":"https://doi.org/10.1177/1087057113510740","title":"Discovery of Enzyme Modulators via High-Throughput Time-Resolved FRET in Living Cells","display_name":"Discovery of Enzyme Modulators via High-Throughput Time-Resolved FRET in Living Cells","publication_year":2014,"publication_date":"2014-01-16","ids":{"openalex":"https://openalex.org/W2098505754","doi":"https://doi.org/10.1177/1087057113510740","mag":"2098505754","pmid":"https://pubmed.ncbi.nlm.nih.gov/24436077","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4013825"},"language":"en","primary_location":{"id":"doi:10.1177/1087057113510740","doi":"https://doi.org/10.1177/1087057113510740","is_oa":true,"landing_page_url":"https://doi.org/10.1177/1087057113510740","pdf_url":"http://slas-discovery.org/article/S2472555222073282/pdf","source":{"id":"https://openalex.org/S4387277810","display_name":"SLAS DISCOVERY","issn_l":"2472-5552","issn":["2472-5552","2472-5560"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-40"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SLAS Discovery","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://slas-discovery.org/article/S2472555222073282/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5025569367","display_name":"Simon J. Gruber","orcid":"https://orcid.org/0000-0002-3411-1759","observed_orcids":["https://orcid.org/0000-0002-3411-1759"]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Simon J. Gruber","raw_affiliation_strings":["1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA","Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA","institution_ids":["https://openalex.org/I130238516"]},{"raw_affiliation_string":"Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA","institution_ids":["https://openalex.org/I130238516"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004618328","display_name":"R\u01cezvan L. Cornea","orcid":"https://orcid.org/0000-0001-5739-0992","observed_orcids":["https://orcid.org/0000-0001-5739-0992","https://orcid.org/0000-0002-3015-2043"]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Razvan L. Cornea","raw_affiliation_strings":["Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA","institution_ids":["https://openalex.org/I130238516"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100421148","display_name":"Ji Li","orcid":"https://orcid.org/0000-0002-0899-4412","observed_orcids":["https://orcid.org/0000-0002-0899-4412"]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ji Li","raw_affiliation_strings":["Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA","institution_ids":["https://openalex.org/I130238516"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024934620","display_name":"Kurt C. Peterson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210103609","display_name":"Fluorescence Innovations (United States)","ror":"https://ror.org/01bqqes77","country_code":"US","type":"company","lineage":["https://openalex.org/I4210103609"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kurt C. Peterson","raw_affiliation_strings":["Fluorescence Innovations, Inc., Minneapolis, MN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fluorescence Innovations, Inc., Minneapolis, MN, USA","institution_ids":["https://openalex.org/I4210103609"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066720305","display_name":"Tory M. Schaaf","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tory M. Schaaf","raw_affiliation_strings":["Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA","institution_ids":["https://openalex.org/I130238516"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028487403","display_name":"Gregory D. Gillispie","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]},{"id":"https://openalex.org/I4210103609","display_name":"Fluorescence Innovations (United States)","ror":"https://ror.org/01bqqes77","country_code":"US","type":"company","lineage":["https://openalex.org/I4210103609"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gregory D. Gillispie","raw_affiliation_strings":["Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA","Fluorescence Innovations, Inc., Minneapolis, MN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA","institution_ids":["https://openalex.org/I130238516"]},{"raw_affiliation_string":"Fluorescence Innovations, Inc., Minneapolis, MN, USA","institution_ids":["https://openalex.org/I4210103609"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070670275","display_name":"Russell S. Dahl","orcid":"https://orcid.org/0000-0002-2939-1004","observed_orcids":["https://orcid.org/0000-0002-2939-1004"]},"institutions":[{"id":"https://openalex.org/I189273616","display_name":"Rosalind Franklin University of Medicine and Science","ror":"https://ror.org/04fegvg32","country_code":"US","type":"education","lineage":["https://openalex.org/I189273616"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Russell Dahl","raw_affiliation_strings":["Department of Pharmaceutical Sciences, Rosalind Franklin University, Chicago, IL, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Sciences, Rosalind Franklin University, Chicago, IL, USA","institution_ids":["https://openalex.org/I189273616"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112098174","display_name":"Krisztina M. Zsebo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210167425","display_name":"Celladon Corporation (United States)","ror":"https://ror.org/05yh2xc78","country_code":"US","type":"company","lineage":["https://openalex.org/I4210167425"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Krisztina M. Zsebo","raw_affiliation_strings":["Celladon Corporation, San Diego, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Celladon Corporation, San Diego, CA, USA","institution_ids":["https://openalex.org/I4210167425"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068004698","display_name":"Seth L. Robia","orcid":"https://orcid.org/0000-0002-1193-9510","observed_orcids":["https://orcid.org/0000-0002-1193-9510"]},"institutions":[{"id":"https://openalex.org/I1925986","display_name":"Loyola University Chicago","ror":"https://ror.org/04b6x2g63","country_code":"US","type":"education","lineage":["https://openalex.org/I1925986"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Seth L. Robia","raw_affiliation_strings":["Department of Cell and Molecular Physiology, Loyola University Chicago, Maywood, IL, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Molecular Physiology, Loyola University Chicago, Maywood, IL, USA","institution_ids":["https://openalex.org/I1925986"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5063616345","display_name":"David Dale Thomas","orcid":"https://orcid.org/0000-0002-8822-2040","observed_orcids":["https://orcid.org/0000-0002-8822-2040","https://orcid.org/0000-0001-5739-0992"]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"David D. Thomas","raw_affiliation_strings":["Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA","institution_ids":["https://openalex.org/I130238516"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5063616345"],"corresponding_institution_ids":["https://openalex.org/I130238516"],"apc_list":{"value":2070,"currency":"USD","value_usd":2070},"apc_paid":{"value":2070,"currency":"USD","value_usd":2070},"fwci":3.1237,"has_fulltext":true,"cited_by_count":102,"citation_normalized_percentile":{"value":0.92299615,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"19","issue":"2","first_page":"215","last_page":"222"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9883000254631042,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9883000254631042,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11204","display_name":"Ion Channels and Receptors","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/2809","display_name":"Sensory Systems"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/serca","display_name":"SERCA","score":0.9589999914169312},{"id":"https://openalex.org/keywords/sarco-endoplasmic-reticulum-calcium-atpase","display_name":"sarco/endoplasmic reticulum calcium ATPase","score":0.9869999885559082},{"id":"https://openalex.org/keywords/enzyme-modulators","display_name":"enzyme modulators","score":0.703000009059906},{"id":"https://openalex.org/keywords/small-molecule-screening","display_name":"small molecule screening","score":0.6729999780654907},{"id":"https://openalex.org/keywords/live-cell-assay","display_name":"live-cell assay","score":0.8980000019073486},{"id":"https://openalex.org/keywords/calcium-pump-inhibition","display_name":"calcium pump inhibition","score":0.5419999957084656},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.6909999847412109},{"id":"https://openalex.org/keywords/hek-cells","display_name":"HEK cells","score":0.48899999260902405}],"concepts":[{"id":"https://openalex.org/C2781414619","wikidata":"https://www.wikidata.org/wiki/Q24785512","display_name":"SERCA","level":4,"score":0.9700249433517456},{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.8391151428222656},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.7320970296859741},{"id":"https://openalex.org/C158617107","wikidata":"https://www.wikidata.org/wiki/Q79927","display_name":"Endoplasmic reticulum","level":2,"score":0.5951676964759827},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.5705565810203552},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5513836145401001},{"id":"https://openalex.org/C2778067846","wikidata":"https://www.wikidata.org/wiki/Q575416","display_name":"Yellow fluorescent protein","level":3,"score":0.5440560579299927},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5344722867012024},{"id":"https://openalex.org/C2778861558","wikidata":"https://www.wikidata.org/wiki/Q3101245","display_name":"Plate reader","level":3,"score":0.47528305649757385},{"id":"https://openalex.org/C51323132","wikidata":"https://www.wikidata.org/wiki/Q626251","display_name":"High-throughput screening","level":2,"score":0.4299900531768799},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.36807799339294434},{"id":"https://openalex.org/C23265538","wikidata":"https://www.wikidata.org/wiki/Q300033","display_name":"ATPase","level":3,"score":0.3563762307167053},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3312234878540039},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.21746277809143066},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.19813916087150574},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.06901928782463074},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000097573","descriptor_name":"Red Fluorescent Protein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015374","descriptor_name":"Biosensing Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D022781","descriptor_name":"Hepatocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D031541","descriptor_name":"Fluorescence Resonance Energy Transfer","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D053498","descriptor_name":"Sarcoplasmic Reticulum Calcium-Transporting ATPases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D053498","descriptor_name":"Sarcoplasmic Reticulum Calcium-Transporting ATPases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D053498","descriptor_name":"Sarcoplasmic Reticulum Calcium-Transporting ATPases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057166","descriptor_name":"High-Throughput Screening Assays","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D057809","descriptor_name":"HEK293 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1177/1087057113510740","doi":"https://doi.org/10.1177/1087057113510740","is_oa":true,"landing_page_url":"https://doi.org/10.1177/1087057113510740","pdf_url":"http://slas-discovery.org/article/S2472555222073282/pdf","source":{"id":"https://openalex.org/S4387277810","display_name":"SLAS DISCOVERY","issn_l":"2472-5552","issn":["2472-5552","2472-5560"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-40"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SLAS Discovery","raw_type":"journal-article"},{"id":"pmid:24436077","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24436077","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biomolecular screening","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4013825","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC4013825/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Biomol Screen","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1177/1087057113510740","doi":"https://doi.org/10.1177/1087057113510740","is_oa":true,"landing_page_url":"https://doi.org/10.1177/1087057113510740","pdf_url":"http://slas-discovery.org/article/S2472555222073282/pdf","source":{"id":"https://openalex.org/S4387277810","display_name":"SLAS DISCOVERY","issn_l":"2472-5552","issn":["2472-5552","2472-5560"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-40"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SLAS Discovery","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1070380227","display_name":"High-throughput screen to discover SERCA activators for heart failure therapy","funder_award_id":"R21AG042996","funder_id":"https://openalex.org/F4320337337","funder_display_name":"National Institute on Aging"},{"id":"https://openalex.org/G3395336405","display_name":"Structure Changes of Ion-motive ATPases","funder_award_id":"R01HL106189","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G7224937801","display_name":"Structural Dynamics of Muscle Calcium ATPase Regulation","funder_award_id":"R01GM027906","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G7747557954","display_name":null,"funder_award_id":"MDA218102","funder_id":"https://openalex.org/F4320332505","funder_display_name":"U.S. Public Health Service"},{"id":"https://openalex.org/G8279109363","display_name":"Muscular Dystrophy Center Core Laboratories","funder_award_id":"P30AR057220","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"},{"id":"https://openalex.org/G9000890370","display_name":"Regulation of Cardiac Calcium Transport","funder_award_id":"R01HL092321","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G9000907930","display_name":"Minnesota Muscle Training Program","funder_award_id":"T32AR007612","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"},{"id":"https://openalex.org/G9000916169","display_name":"Calmodulin and Regulation of Cardiac Ryanodine Receptor and CaMKII","funder_award_id":"R01HL092097","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320306230","display_name":"American Heart Association","ror":"https://ror.org/013kjyp64"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332505","display_name":"U.S. Public Health Service","ror":"https://ror.org/05xf94514"},{"id":"https://openalex.org/F4320337337","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337362","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2098505754.pdf","grobid_xml":"https://content.openalex.org/works/W2098505754.grobid-xml"},"referenced_works_count":30,"referenced_works":["https://openalex.org/W1567587576","https://openalex.org/W1932843942","https://openalex.org/W1966103529","https://openalex.org/W1967021616","https://openalex.org/W1979477410","https://openalex.org/W1980712596","https://openalex.org/W1986324389","https://openalex.org/W1991001144","https://openalex.org/W2011127005","https://openalex.org/W2012327268","https://openalex.org/W2022534774","https://openalex.org/W2038217580","https://openalex.org/W2047003541","https://openalex.org/W2050824263","https://openalex.org/W2076071130","https://openalex.org/W2077209938","https://openalex.org/W2084215755","https://openalex.org/W2090055042","https://openalex.org/W2090338856","https://openalex.org/W2092175923","https://openalex.org/W2111464978","https://openalex.org/W2123490348","https://openalex.org/W2124034869","https://openalex.org/W2128127545","https://openalex.org/W2141136810","https://openalex.org/W2153974854","https://openalex.org/W2156314083","https://openalex.org/W2437613310","https://openalex.org/W2792980560","https://openalex.org/W4232999120"],"related_works":["https://openalex.org/W2023023427","https://openalex.org/W3004856225","https://openalex.org/W4382795567","https://openalex.org/W4243084772","https://openalex.org/W4321486634","https://openalex.org/W2017185209","https://openalex.org/W2978535871","https://openalex.org/W2791768052","https://openalex.org/W2094319153","https://openalex.org/W2156314083"],"abstract_inverted_index":{"We":[0,107],"have":[1],"used":[2],"a":[3,12,22,109,134],"\"two-color\"":[4],"SERCA":[5,34,37,105,121,132],"(sarco/endoplasmic":[6],"reticulum":[7],"calcium":[8],"ATPase)":[9],"biosensor":[10],"and":[11,51,113,130,145],"unique":[13],"high-throughput":[14],"fluorescence":[15],"lifetime":[16],"plate":[17,94],"reader":[18],"(FLT-PR)":[19],"to":[20,27,47,56,81,103,148],"develop":[21],"high-precision":[23],"live-cell":[24],"assay":[25,138],"designed":[26],"screen":[28],"for":[29,141],"small":[30,110],"molecules":[31],"that":[32,68,117],"perturb":[33],"structure.":[35],"A":[36],"construct,":[38],"in":[39,63,70,85,133],"which":[40,88],"red":[41],"fluorescent":[42,53],"protein":[43,54],"(RFP)":[44],"was":[45,60,83,100],"fused":[46],"the":[48,86],"N":[49],"terminus":[50],"green":[52],"(GFP)":[55],"an":[57,64],"interior":[58],"loop,":[59],"stably":[61],"expressed":[62],"HEK":[65],"cell":[66],"line":[67],"grows":[69],"monolayer":[71],"or":[72],"suspension.":[73],"Fluorescence":[74],"resonance":[75],"energy":[76],"transfer":[77],"(FRET)":[78],"from":[79],"GFP":[80],"RFP":[82],"measured":[84],"FLT-PR,":[87],"increases":[89],"precision":[90],"30-fold":[91],"over":[92],"intensity-based":[93],"readers":[95],"without":[96],"sacrificing":[97],"throughput.":[98],"FRET":[99,129],"highly":[101],"sensitive":[102],"known":[104],"modulators.":[106],"screened":[108],"chemical":[111],"library":[112],"identified":[114],"10":[115],"compounds":[116,126],"significantly":[118],"affected":[119],"two-color":[120],"FLT.":[122],"Three":[123],"of":[124],"these":[125],"reproducibly":[127],"lowered":[128],"inhibited":[131],"dose-dependent":[135],"manner.":[136],"This":[137],"is":[139,146],"ready":[140],"large-scale":[142],"HTS":[143],"campaigns":[144],"adaptable":[147],"many":[149],"other":[150],"targets.":[151]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":8},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":13},{"year":2022,"cited_by_count":12},{"year":2021,"cited_by_count":9},{"year":2020,"cited_by_count":13},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":13},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":8},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}