{"id":"https://openalex.org/W2098158296","doi":"https://doi.org/10.1002/pros.20997","title":"A novel, spontaneously immortalized, human prostate cancer cell line, Bob, offers a unique model for pre\u2010clinical prostate cancer studies","display_name":"A novel, spontaneously immortalized, human prostate cancer cell line, Bob, offers a unique model for pre\u2010clinical prostate cancer studies","publication_year":2009,"publication_date":"2009-06-18","ids":{"openalex":"https://openalex.org/W2098158296","doi":"https://doi.org/10.1002/pros.20997","mag":"2098158296","pmid":"https://pubmed.ncbi.nlm.nih.gov/19544327"},"language":"en","primary_location":{"id":"doi:10.1002/pros.20997","doi":"https://doi.org/10.1002/pros.20997","is_oa":false,"landing_page_url":"https://doi.org/10.1002/pros.20997","pdf_url":null,"source":{"id":"https://openalex.org/S197000376","display_name":"The Prostate","issn_l":"0270-4137","issn":["0270-4137","1097-0045"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Prostate","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000156180","display_name":"Gerhardt Attard","orcid":"https://orcid.org/0000-0002-4811-7983","observed_orcids":["https://orcid.org/0000-0002-4811-7983"]},"institutions":[{"id":"https://openalex.org/I1325846038","display_name":"Royal Marsden NHS Foundation Trust","ror":"https://ror.org/0008wzh48","country_code":"GB","type":"healthcare","lineage":["https://openalex.org/I1325846038"]},{"id":"https://openalex.org/I142947616","display_name":"Institute of Cancer Research","ror":"https://ror.org/043jzw605","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I142947616"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Gerhardt Attard","raw_affiliation_strings":["The Institute of Cancer Research, Surrey, UK","The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK","The Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey, SM2 5PT, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Institute of Cancer Research, Surrey, UK","institution_ids":["https://openalex.org/I142947616"]},{"raw_affiliation_string":"The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK","institution_ids":["https://openalex.org/I142947616"]},{"raw_affiliation_string":"The Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey, SM2 5PT, UK","institution_ids":["https://openalex.org/I1325846038"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012544394","display_name":"Si\u00e2n Rizzo","orcid":"https://orcid.org/0000-0003-3072-8724","observed_orcids":["https://orcid.org/0000-0003-3072-8724"]},"institutions":[{"id":"https://openalex.org/I142947616","display_name":"Institute of Cancer Research","ror":"https://ror.org/043jzw605","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I142947616"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Sian Rizzo","raw_affiliation_strings":["The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK","institution_ids":["https://openalex.org/I142947616"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078756581","display_name":"Ioanna Ledaki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I142947616","display_name":"Institute of Cancer Research","ror":"https://ror.org/043jzw605","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I142947616"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Ioanna Ledaki","raw_affiliation_strings":["The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK","institution_ids":["https://openalex.org/I142947616"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109994256","display_name":"Jeremy Clark","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I142947616","display_name":"Institute of Cancer Research","ror":"https://ror.org/043jzw605","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I142947616"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Jeremy Clark","raw_affiliation_strings":["The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK","institution_ids":["https://openalex.org/I142947616"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103901047","display_name":"Alison H.M. Reid","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1325846038","display_name":"Royal Marsden NHS Foundation Trust","ror":"https://ror.org/0008wzh48","country_code":"GB","type":"healthcare","lineage":["https://openalex.org/I1325846038"]},{"id":"https://openalex.org/I142947616","display_name":"Institute of Cancer Research","ror":"https://ror.org/043jzw605","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I142947616"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Alison H.M. Reid","raw_affiliation_strings":["The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK","The Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey, SM2 5PT, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK","institution_ids":["https://openalex.org/I142947616"]},{"raw_affiliation_string":"The Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey, SM2 5PT, UK","institution_ids":["https://openalex.org/I1325846038"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087992820","display_name":"Alan C. Thompson","orcid":"https://orcid.org/0000-0003-1400-0098","observed_orcids":["https://orcid.org/0000-0003-1400-0098"]},"institutions":[{"id":"https://openalex.org/I1325846038","display_name":"Royal Marsden NHS Foundation Trust","ror":"https://ror.org/0008wzh48","country_code":"GB","type":"healthcare","lineage":["https://openalex.org/I1325846038"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Alan Thompson","raw_affiliation_strings":["The Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey, SM2 5PT, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey, SM2 5PT, UK","institution_ids":["https://openalex.org/I1325846038"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080362183","display_name":"Vincent S Khoo","orcid":"https://orcid.org/0000-0002-0763-6832","observed_orcids":["https://orcid.org/0000-0002-0763-6832"]},"institutions":[{"id":"https://openalex.org/I1325846038","display_name":"Royal Marsden NHS Foundation Trust","ror":"https://ror.org/0008wzh48","country_code":"GB","type":"healthcare","lineage":["https://openalex.org/I1325846038"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Vincent Khoo","raw_affiliation_strings":["The Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey, SM2 5PT, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey, SM2 5PT, UK","institution_ids":["https://openalex.org/I1325846038"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072378199","display_name":"Johann Sebastian De Bono","orcid":"https://orcid.org/0000-0002-2034-595X","observed_orcids":["https://orcid.org/0000-0002-2034-595X"]},"institutions":[{"id":"https://openalex.org/I1325846038","display_name":"Royal Marsden NHS Foundation Trust","ror":"https://ror.org/0008wzh48","country_code":"GB","type":"healthcare","lineage":["https://openalex.org/I1325846038"]},{"id":"https://openalex.org/I142947616","display_name":"Institute of Cancer Research","ror":"https://ror.org/043jzw605","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I142947616"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Johann S. de Bono","raw_affiliation_strings":["The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK","The Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey, SM2 5PT, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK","institution_ids":["https://openalex.org/I142947616"]},{"raw_affiliation_string":"The Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey, SM2 5PT, UK","institution_ids":["https://openalex.org/I1325846038"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104097760","display_name":"Colin S. Cooper","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I142947616","display_name":"Institute of Cancer Research","ror":"https://ror.org/043jzw605","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I142947616"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Colin S. Cooper","raw_affiliation_strings":["The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK","institution_ids":["https://openalex.org/I142947616"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111975996","display_name":"David L. Hudson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I142947616","display_name":"Institute of Cancer Research","ror":"https://ror.org/043jzw605","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I142947616"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"David L. Hudson","raw_affiliation_strings":["The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK","institution_ids":["https://openalex.org/I142947616"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5111975996"],"corresponding_institution_ids":["https://openalex.org/I142947616"],"apc_list":{"value":4600,"currency":"USD","value_usd":4600},"apc_paid":null,"fwci":1.4024,"has_fulltext":false,"cited_by_count":13,"citation_normalized_percentile":{"value":0.83922749,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"69","issue":"14","first_page":"1507","last_page":"1520"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10543","display_name":"Prostate Cancer Treatment and Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10543","display_name":"Prostate Cancer Treatment and Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10336","display_name":"Cancer Cells and Metastasis","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10124","display_name":"Prostate Cancer Diagnosis and Treatment","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/castration-resistant-prostate-cancer","display_name":"castration-resistant prostate cancer","score":0.9739999771118164},{"id":"https://openalex.org/keywords/prostate-cancer-cell-lines","display_name":"prostate cancer cell lines","score":0.8180000185966492},{"id":"https://openalex.org/keywords/spontaneous-immortalization","display_name":"spontaneous immortalization","score":0.8980000019073486},{"id":"https://openalex.org/keywords/transrectal-needle-biopsy","display_name":"transrectal needle biopsy","score":0.8240000009536743},{"id":"https://openalex.org/keywords/methylcellulose","display_name":"methylcellulose","score":0.7570000290870667},{"id":"https://openalex.org/keywords/comparative-genomic-hybridization","display_name":"comparative genomic hybridization","score":0.7620000243186951},{"id":"https://openalex.org/keywords/p53-expression","display_name":"p53 expression","score":0.7720000147819519},{"id":"https://openalex.org/keywords/prostate-stem-cell-antigen","display_name":"prostate stem cell antigen","score":0.6740000247955322},{"id":"https://openalex.org/keywords/prostatic-acid-phosphatase","display_name":"prostatic acid phosphatase","score":0.8349999785423279},{"id":"https://openalex.org/keywords/erg-rearrangement","display_name":"ERG rearrangement","score":0.7749999761581421},{"id":"https://openalex.org/keywords/basal-like-phenotype","display_name":"basal-like phenotype","score":0.6830000281333923},{"id":"https://openalex.org/keywords/tumor-invasion","display_name":"tumor invasion","score":0.5979999899864197},{"id":"https://openalex.org/keywords/preclinical-cancer-models","display_name":"preclinical cancer models","score":0.5440000295639038}],"concepts":[{"id":"https://openalex.org/C2780192828","wikidata":"https://www.wikidata.org/wiki/Q181257","display_name":"Prostate cancer","level":3,"score":0.8174425959587097},{"id":"https://openalex.org/C2776235491","wikidata":"https://www.wikidata.org/wiki/Q9625","display_name":"Prostate","level":3,"score":0.7241722941398621},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.5909062027931213},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.5527809262275696},{"id":"https://openalex.org/C2994372470","wikidata":"https://www.wikidata.org/wiki/Q4118072","display_name":"Cancer cell lines","level":4,"score":0.4892439842224121},{"id":"https://openalex.org/C6045943","wikidata":"https://www.wikidata.org/wiki/Q21014462","display_name":"Immortalised cell line","level":3,"score":0.46781229972839355},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.40968361496925354},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.3467687666416168},{"id":"https://openalex.org/C143998085","wikidata":"https://www.wikidata.org/wiki/Q162555","display_name":"Oncology","level":1,"score":0.34340453147888184},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.3426366448402405},{"id":"https://openalex.org/C96232424","wikidata":"https://www.wikidata.org/wiki/Q4118072","display_name":"Cancer cell","level":3,"score":0.3316355347633362},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3309546113014221},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.26733922958374023},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001707","descriptor_name":"Biopsy, Needle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001798","descriptor_name":"Blood Proteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002448","descriptor_name":"Cell Adhesion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002461","descriptor_name":"Cell Line, Transformed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002465","descriptor_name":"Cell Movement","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004273","descriptor_name":"DNA, Neoplasm","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007621","descriptor_name":"Karyotyping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008747","descriptor_name":"Methylcellulose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009368","descriptor_name":"Neoplasm Transplantation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011471","descriptor_name":"Prostatic Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011471","descriptor_name":"Prostatic Neoplasms","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D016513","descriptor_name":"Mice, SCID","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018874","descriptor_name":"Spheroids, Cellular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D039621","descriptor_name":"Eukaryotic Initiation Factor-3","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055028","descriptor_name":"Comparative Genomic Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1002/pros.20997","doi":"https://doi.org/10.1002/pros.20997","is_oa":false,"landing_page_url":"https://doi.org/10.1002/pros.20997","pdf_url":null,"source":{"id":"https://openalex.org/S197000376","display_name":"The Prostate","issn_l":"0270-4137","issn":["0270-4137","1097-0045"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Prostate","raw_type":"journal-article"},{"id":"pmid:19544327","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19544327","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Prostate","raw_type":"Journal Article"},{"id":"pmh:oai:ueaeprints.uea.ac.uk:46142","doi":null,"is_oa":false,"landing_page_url":"https://ueaeprints.uea.ac.uk/id/eprint/46142/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400384","display_name":"UEA Digital Repository (University of East Anglia)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1118541","host_organization_name":"University of East Anglia","host_organization_lineage":["https://openalex.org/I1118541"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2519382346","display_name":null,"funder_award_id":"CL-2008-22-001","funder_id":"https://openalex.org/F4320319990","funder_display_name":"National Institute for Health and Care Research"},{"id":"https://openalex.org/G8217253432","display_name":null,"funder_award_id":"C51/A7401","funder_id":"https://openalex.org/F4320319985","funder_display_name":"Cancer Research UK"}],"funders":[{"id":"https://openalex.org/F4320319985","display_name":"Cancer Research UK","ror":"https://ror.org/054225q67"},{"id":"https://openalex.org/F4320319990","display_name":"National Institute for Health and Care Research","ror":"https://ror.org/0187kwz08"},{"id":"https://openalex.org/F4320328946","display_name":"Stichting Wetenschappelijk Onderzoek Prostaatkanker","ror":"https://ror.org/01hthf597"},{"id":"https://openalex.org/F5948211726","display_name":"Bob Champion Cancer Trust","ror":"https://ror.org/00cm8a840"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W39809497","https://openalex.org/W1964943167","https://openalex.org/W2003626435","https://openalex.org/W2017329417","https://openalex.org/W2021563592","https://openalex.org/W2024733621","https://openalex.org/W2039169692","https://openalex.org/W2053490054","https://openalex.org/W2056480903","https://openalex.org/W2058430797","https://openalex.org/W2081290486","https://openalex.org/W2081484834","https://openalex.org/W2111516286","https://openalex.org/W2117936087","https://openalex.org/W2122968754","https://openalex.org/W2124994950","https://openalex.org/W2137330819","https://openalex.org/W2150066245","https://openalex.org/W2153382986","https://openalex.org/W2154165363","https://openalex.org/W2155679349","https://openalex.org/W2441539154"],"related_works":["https://openalex.org/W2051420134","https://openalex.org/W1582302429","https://openalex.org/W2091569641","https://openalex.org/W2443673815","https://openalex.org/W4396642361","https://openalex.org/W2000019750","https://openalex.org/W2172154317","https://openalex.org/W92453867","https://openalex.org/W2322250462","https://openalex.org/W2415025943"],"abstract_inverted_index":{"INTRODUCTION:":[0],"New":[1],"in":[2,59,106,113,135,138,176,187,223],"vitro":[3],"models":[4],"of":[5,18,54,73,79,85,120,124,148,155,159,209],"castration-resistant":[6],"prostate":[7,20,115,168,199],"cancer":[8,200],"(CRPC)":[9],"are":[10],"urgently":[11],"required.":[12],"METHODS:":[13],"Trans-rectal":[14],"needle":[15],"biopsies":[16],"(TRBP)":[17],"the":[19,37,145,195,229],"were":[21,40,69,90,126,183],"performed":[22],"for":[23,220,227],"research":[24],"purposes":[25],"on":[26,128],"progressing":[27],"CRPC":[28,224,230],"patients":[29,68],"who":[30],"had":[31],"not":[32,184],"received":[33],"prior":[34],"treatment":[35],"to":[36,203],"prostate.":[38],"Biopsies":[39,65],"immediately":[41],"digested":[42],"with":[43,50,212],"collagenase":[44],"and":[45,57,71,109,122,132,140,157,167,179,181,225],"plated":[46],"onto":[47],"collagen-coated":[48],"flasks":[49],"a":[51,94,207,210,216],"feeder":[52],"layer":[53],"3T6":[55],"cells":[56],"cultured":[58],"cytokine-supplemented":[60],"keratinocyte":[61],"serum-free":[62],"medium.":[63],"RESULTS:":[64],"from":[66,93,206],"25":[67],"collected":[70],"one":[72],"these,":[74],"following":[75],"an":[76],"initial":[77],"period":[78],"crisis,":[80],"spontaneously":[81,197],"immortalized.":[82],"A":[83],"series":[84],"cell":[86,170,201],"lines":[87],"called":[88],"Bob":[89,151,214],"then":[91],"established":[92,205],"clone":[95],"that":[96],"survived":[97],"CD133-selection":[98],"followed":[99],"by":[100],"4":[101],"weeks":[102],"under":[103],"adhesion-independent":[104],"conditions":[105],"methylcellulose.":[107],"Gains":[108],"losses":[110],"previously":[111],"described":[112],"clinical":[114],"tumors,":[116],"most":[117],"notably":[118],"loss":[119],"8(p)":[121],"gain":[123],"8(q),":[125],"identified":[127],"comparative":[129],"genomic":[130],"hybridization":[131],"long-term":[133],"growth":[134,178],"culture,":[136],"survival":[137],"methylcellulose":[139],"invasion":[141],"through":[142],"matrigel":[143],"confirmed":[144],"malignant":[146],"phenotype":[147],"Bob.":[149],"Furthermore,":[150],"expressed":[152],"high":[153],"levels":[154],"p53":[156],"markers":[158],"early":[160],"differentiation,":[161],"including":[162],"K8,":[163],"prostatic":[164],"acid":[165],"phosphatase":[166],"stem":[169],"antigen.":[171],"There":[172],"was,":[173],"however,":[174],"no":[175],"vivo":[177],"ERG":[180],"ETV1":[182],"rearranged.":[185],"Growth":[186],"serum":[188],"permitted":[189],"some":[190],"differentiation.":[191],"CONCLUSION:":[192],"This":[193],"is":[194,215],"first":[196],"immortalized":[198],"line":[202],"be":[204],"TRBP":[208],"patient":[211],"CRPC.":[213],"novel":[217],"pre-clinical":[218],"model":[219],"functional":[221],"studies":[222],"especially":[226],"studying":[228],"\"basal\"":[231],"phenotype.":[232]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}