{"id":"https://openalex.org/W2098034398","doi":null,"title":"Oxidative stress-induced, peroxynitrite-dependent, modifications of myosin light chain 1 lead to its increased degradation by matrix metalloproteinase-2","display_name":"Oxidative stress-induced, peroxynitrite-dependent, modifications of myosin light chain 1 lead to its increased degradation by matrix metalloproteinase-2","publication_year":2010,"publication_date":"2010-06-01","ids":{"openalex":"https://openalex.org/W2098034398","mag":"2098034398"},"language":"en","primary_location":{"id":"pmh:oai:ecommons.usask.ca:10388/etd-06232010-154218","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10388/etd-06232010-154218","pdf_url":"https://harvest.usask.ca/bitstreams/ef6a172f-b573-488e-afd7-f2d57466901d/download","source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://harvest.usask.ca/bitstreams/ef6a172f-b573-488e-afd7-f2d57466901d/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5071707467","display_name":"Dorota Polewicz","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Dorota Polewicz","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5071707467"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.32100000977516174,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.32100000977516174,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11057","display_name":"Cardiac Ischemia and Reperfusion","score":0.3158999979496002,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10155","display_name":"Nitric Oxide and Endothelin Effects","score":0.2020999938249588,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/myosin-light-chain-1","display_name":"myosin light chain 1","score":0.9990000128746033},{"id":"https://openalex.org/keywords/peroxynitrite","display_name":"peroxynitrite","score":0.9070000052452087},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.921999990940094},{"id":"https://openalex.org/keywords/mmp-2","display_name":"MMP-2","score":0.996999979019165},{"id":"https://openalex.org/keywords/protein-degradation","display_name":"protein degradation","score":0.8299999833106995},{"id":"https://openalex.org/keywords/nitration","display_name":"nitration","score":0.8309999704360962},{"id":"https://openalex.org/keywords/nitrosylation","display_name":"nitrosylation","score":0.996999979019165},{"id":"https://openalex.org/keywords/post-translational-modification","display_name":"post-translational modification","score":0.8550000190734863},{"id":"https://openalex.org/keywords/cardiac-contractile-dysfunction","display_name":"cardiac contractile dysfunction","score":0.7480000257492065},{"id":"https://openalex.org/keywords/myocardial-ischemia","display_name":"myocardial ischemia","score":0.8450000286102295},{"id":"https://openalex.org/keywords/hypoxia-reoxygenation","display_name":"hypoxia/reoxygenation","score":0.9110000133514404},{"id":"https://openalex.org/keywords/fetpps","display_name":"FeTPPS","score":0.8740000128746033},{"id":"https://openalex.org/keywords/phenanthroline","display_name":"phenanthroline","score":0.9380000233650208},{"id":"https://openalex.org/keywords/cardiomyocyte-contractility","display_name":"cardiomyocyte contractility","score":0.8659999966621399}],"concepts":[{"id":"https://openalex.org/C2778784160","wikidata":"https://www.wikidata.org/wiki/Q419130","display_name":"Peroxynitrite","level":4,"score":0.8085645437240601},{"id":"https://openalex.org/C2776151105","wikidata":"https://www.wikidata.org/wiki/Q898814","display_name":"Oxidative stress","level":2,"score":0.6478961110115051},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6246594190597534},{"id":"https://openalex.org/C2779679103","wikidata":"https://www.wikidata.org/wiki/Q5251805","display_name":"Degradation (telecommunications)","level":2,"score":0.6158522963523865},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5506139397621155},{"id":"https://openalex.org/C6997183","wikidata":"https://www.wikidata.org/wiki/Q271727","display_name":"Myosin","level":2,"score":0.5206462740898132},{"id":"https://openalex.org/C109523444","wikidata":"https://www.wikidata.org/wiki/Q424216","display_name":"Matrix metalloproteinase","level":2,"score":0.5000450611114502},{"id":"https://openalex.org/C8035138","wikidata":"https://www.wikidata.org/wiki/Q6017854","display_name":"Myosin light-chain kinase","level":3,"score":0.4958983361721039},{"id":"https://openalex.org/C106487976","wikidata":"https://www.wikidata.org/wiki/Q685816","display_name":"Matrix (chemical analysis)","level":2,"score":0.4501658082008362},{"id":"https://openalex.org/C21036866","wikidata":"https://www.wikidata.org/wiki/Q181767","display_name":"Stress (linguistics)","level":2,"score":0.4328065514564514},{"id":"https://openalex.org/C57600042","wikidata":"https://www.wikidata.org/wiki/Q227564","display_name":"Oxidative phosphorylation","level":2,"score":0.43116477131843567},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3390398621559143},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.32694998383522034},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.13957840204238892},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.12675711512565613},{"id":"https://openalex.org/C2780795997","wikidata":"https://www.wikidata.org/wiki/Q413283","display_name":"Superoxide","level":3,"score":0.09653761982917786},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.09388324618339539},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:ecommons.usask.ca:10388/etd-06232010-154218","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10388/etd-06232010-154218","pdf_url":"https://harvest.usask.ca/bitstreams/ef6a172f-b573-488e-afd7-f2d57466901d/download","source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},{"id":"pmh:oai:harvest.usask.ca:10388/etd-06232010-154218","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/10388/etd-06232010-154218","pdf_url":null,"source":{"id":"https://openalex.org/S7407063267","display_name":"HARVEST (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:2098034398","doi":null,"is_oa":false,"landing_page_url":"https://harvest.usask.ca/bitstream/10388/etd-06232010-154218/1/Polewicz_Dorota_2010.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:ecommons.usask.ca:10388/etd-06232010-154218","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10388/etd-06232010-154218","pdf_url":"https://harvest.usask.ca/bitstreams/ef6a172f-b573-488e-afd7-f2d57466901d/download","source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8560000061988831}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2098034398.pdf","grobid_xml":"https://content.openalex.org/works/W2098034398.grobid-xml"},"referenced_works_count":179,"referenced_works":["https://openalex.org/W3543680","https://openalex.org/W1038424662","https://openalex.org/W1483108854","https://openalex.org/W1498962226","https://openalex.org/W1505685265","https://openalex.org/W1510906241","https://openalex.org/W1516918404","https://openalex.org/W1519128654","https://openalex.org/W1519472981","https://openalex.org/W1527507383","https://openalex.org/W1541878890","https://openalex.org/W1571553935","https://openalex.org/W1578747264","https://openalex.org/W1585830496","https://openalex.org/W1619234695","https://openalex.org/W1945990234","https://openalex.org/W1963590825","https://openalex.org/W1964994147","https://openalex.org/W1967582474","https://openalex.org/W1967729440","https://openalex.org/W1969773420","https://openalex.org/W1969877720","https://openalex.org/W1969983455","https://openalex.org/W1970604462","https://openalex.org/W1971653265","https://openalex.org/W1973838961","https://openalex.org/W1974358158","https://openalex.org/W1975594408","https://openalex.org/W1977127210","https://openalex.org/W1977368722","https://openalex.org/W1977590844","https://openalex.org/W1978839418","https://openalex.org/W1978942142","https://openalex.org/W1979390430","https://openalex.org/W1981593008","https://openalex.org/W1982054375","https://openalex.org/W1984584032","https://openalex.org/W1985540047","https://openalex.org/W1986884128","https://openalex.org/W1988682256","https://openalex.org/W1988720271","https://openalex.org/W1990031010","https://openalex.org/W1990980324","https://openalex.org/W1992019019","https://openalex.org/W1992962942","https://openalex.org/W1995319149","https://openalex.org/W1995457093","https://openalex.org/W1996969615","https://openalex.org/W1998288051","https://openalex.org/W1998567489","https://openalex.org/W2000479400","https://openalex.org/W2001603814","https://openalex.org/W2001669423","https://openalex.org/W2002909335","https://openalex.org/W2003253443","https://openalex.org/W2004748168","https://openalex.org/W2004867943","https://openalex.org/W2005120762","https://openalex.org/W2006153627","https://openalex.org/W2009279488","https://openalex.org/W2010027735","https://openalex.org/W2011405959","https://openalex.org/W2011612432","https://openalex.org/W2017068027","https://openalex.org/W2019091990","https://openalex.org/W2021457420","https://openalex.org/W2022268100","https://openalex.org/W2022759249","https://openalex.org/W2024605566","https://openalex.org/W2028513366","https://openalex.org/W2029067920","https://openalex.org/W2030756147","https://openalex.org/W2031153770","https://openalex.org/W2033105576","https://openalex.org/W2036547106","https://openalex.org/W2037694436","https://openalex.org/W2038181981","https://openalex.org/W2043355876","https://openalex.org/W2046398732","https://openalex.org/W2046562398","https://openalex.org/W2047015493","https://openalex.org/W2048322342","https://openalex.org/W2049220062","https://openalex.org/W2049636133","https://openalex.org/W2050206397","https://openalex.org/W2051319531","https://openalex.org/W2052260508","https://openalex.org/W2052503231","https://openalex.org/W2053450572","https://openalex.org/W2058086138","https://openalex.org/W2058137293","https://openalex.org/W2058791421","https://openalex.org/W2059363473","https://openalex.org/W2060358638","https://openalex.org/W2060992579","https://openalex.org/W2064216004","https://openalex.org/W2065169761","https://openalex.org/W2065773243","https://openalex.org/W2066548975","https://openalex.org/W2069953906","https://openalex.org/W2071359561","https://openalex.org/W2072299986","https://openalex.org/W2075071669","https://openalex.org/W2076208252","https://openalex.org/W2076708176","https://openalex.org/W2077737947","https://openalex.org/W2078519193","https://openalex.org/W2079785257","https://openalex.org/W2081515380","https://openalex.org/W2081622782","https://openalex.org/W2082582644","https://openalex.org/W2083089504","https://openalex.org/W2084936676","https://openalex.org/W2086251702","https://openalex.org/W2086881564","https://openalex.org/W2088638481","https://openalex.org/W2089399700","https://openalex.org/W2090135952","https://openalex.org/W2091100304","https://openalex.org/W2096499490","https://openalex.org/W2096763491","https://openalex.org/W2097121383","https://openalex.org/W2097124761","https://openalex.org/W2100371150","https://openalex.org/W2100837269","https://openalex.org/W2102622687","https://openalex.org/W2103745677","https://openalex.org/W2105677812","https://openalex.org/W2107893282","https://openalex.org/W2109658199","https://openalex.org/W2110238986","https://openalex.org/W2110565948","https://openalex.org/W2111015914","https://openalex.org/W2111446613","https://openalex.org/W2111718849","https://openalex.org/W2112968592","https://openalex.org/W2115946769","https://openalex.org/W2117327744","https://openalex.org/W2117571600","https://openalex.org/W2118345088","https://openalex.org/W2122270338","https://openalex.org/W2122386753","https://openalex.org/W2124598877","https://openalex.org/W2126910504","https://openalex.org/W2128635872","https://openalex.org/W2129787534","https://openalex.org/W2129812461","https://openalex.org/W2132855020","https://openalex.org/W2135124706","https://openalex.org/W2135289666","https://openalex.org/W2139328007","https://openalex.org/W2139491374","https://openalex.org/W2142206203","https://openalex.org/W2145047965","https://openalex.org/W2145309926","https://openalex.org/W2148075660","https://openalex.org/W2150304491","https://openalex.org/W2154663686","https://openalex.org/W2155169902","https://openalex.org/W2157553830","https://openalex.org/W2162461126","https://openalex.org/W2164160223","https://openalex.org/W2164656801","https://openalex.org/W2164721721","https://openalex.org/W2166085923","https://openalex.org/W2167709602","https://openalex.org/W2168649322","https://openalex.org/W2169095915","https://openalex.org/W2170295209","https://openalex.org/W2183019695","https://openalex.org/W2226676844","https://openalex.org/W2277824238","https://openalex.org/W2402816888","https://openalex.org/W2404387463","https://openalex.org/W2410168531","https://openalex.org/W2439481878","https://openalex.org/W2461370106","https://openalex.org/W2604232804","https://openalex.org/W4388324919"],"related_works":["https://openalex.org/W2065835031","https://openalex.org/W2080638516","https://openalex.org/W1557521764","https://openalex.org/W1998567489","https://openalex.org/W2314544641","https://openalex.org/W2164721721","https://openalex.org/W2017847073","https://openalex.org/W2017068027","https://openalex.org/W1974893888","https://openalex.org/W1969773420"],"abstract_inverted_index":{"Damage":[0],"to":[1,38,75,92,135,154,189],"cardiac":[2,29,64,162],"contractile":[3,251,257],"proteins":[4,252],"such":[5,21],"as":[6,22],"myosin":[7],"light":[8],"chain":[9],"1":[10],"(MLC1),":[11],"during":[12,58,195,216,253],"oxidative":[13,59],"stress":[14,60],"is":[15,37,82,180],"mediated":[16],"by":[17,51,79,84,96,123,145,218],"reactive":[18],"oxygen":[19],"species":[20],"peroxynitrite":[23],"(ONOO-),":[24],"resulting":[25],"in":[26,104,164,168,176],"impairment":[27],"of":[28,34,42,46,101,113,118,129,149,178,201,213,226,235,248,250,256],"systolic":[30],"function.":[31,65],"The":[32,238],"purpose":[33],"this":[35,192],"study":[36,69],"investigate":[39],"the":[40,43,52,67,77,136,157,173,183,199,219,246,254],"effects":[41],"increased":[44,94],"level":[45],"ONOO-":[47,85,220],"on":[48,245],"MLC1":[49,81,119,179,214],"degradation":[50,95,122,177],"proteolytic":[53],"enzyme":[54],"matrix":[55],"metalloproteinase-2":[56],"(MMP-2)":[57],"which":[61,80],"ultimately":[62],"decreases":[63],"In":[66,98],"present":[68],"two":[70],"distinct":[71],"models":[72],"were":[73,187],"utilized":[74],"demonstrate":[76],"mechanism":[78,159,193],"modified":[83],"and":[86,111,115,171],"how":[87],"these":[88,143],"post-translational":[89],"modifications":[90],"lead":[91],"its":[93,121],"MMP-2.":[97,124,140],"a":[99,130,147],"model":[100,148],"newborn":[102,165],"hypoxia-reoxygenation":[103],"piglets":[105,166],"we":[106,126,186,204],"demonstrated":[107],"that":[108,156,172,191,207],"ONOO--induced":[109],"nitration":[110,128],"nitrosylation":[112],"tyrosine":[114,131],"cysteine":[116],"residues":[117],"increase":[120],"Furthermore,":[125,203],"found":[127,206],"residue":[132],"located":[133,181],"adjacent":[134],"cleavage":[137],"site":[138],"for":[139,161],"We":[141],"verified":[142],"results":[144],"using":[146],"isolated":[150,169],"rat":[151],"heart":[152],"myocytes":[153,170],"determine":[155,190],"same":[158],"responsible":[160],"dysfunction":[163],"occurs":[167,194],"MMP-2":[174,227],"involved":[175],"within":[182],"myocytes.":[184],"Moreover,":[185],"able":[188],"ischemia":[196,217],"itself":[197],"before":[198],"onset":[200],"reperfusion.":[202],"have":[205],"pharmacological":[208],"intervention":[209],"aimed":[210],"at":[211],"inhibition":[212],"nitration/nitrosylation":[215],"scavenger":[221],"FeTPPS":[222],"(5,10,15,20-tetrakis-[4-sulfonatophenyl]-porphyrinato-iron[III]),":[223],"or":[224],"inhition":[225],"activity":[228],"with":[229],"phenanthroline,":[230],"provides":[231,242],"an":[232],"effective":[233],"protection":[234],"cardiomyocyte":[236],"contractility.":[237],"work":[239],"presented":[240],"here":[241],"new":[243],"evidence":[244],"mechanisms":[247],"regulation":[249],"development":[255],"dysfunction.":[258]},"counts_by_year":[{"year":2023,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}