{"id":"https://openalex.org/W2097646133","doi":"https://doi.org/10.1093/protein/15.10.827","title":"Design of a monomeric human glutathione transferase GSTP1, a structurally stable but catalytically inactive protein","display_name":"Design of a monomeric human glutathione transferase GSTP1, a structurally stable but catalytically inactive protein","publication_year":2002,"publication_date":"2002-10-01","ids":{"openalex":"https://openalex.org/W2097646133","doi":"https://doi.org/10.1093/protein/15.10.827","mag":"2097646133","pmid":"https://pubmed.ncbi.nlm.nih.gov/12468717"},"language":"en","primary_location":{"id":"doi:10.1093/protein/15.10.827","doi":"https://doi.org/10.1093/protein/15.10.827","is_oa":false,"landing_page_url":"https://doi.org/10.1093/protein/15.10.827","pdf_url":null,"source":{"id":"https://openalex.org/S44492648","display_name":"Protein Engineering Design and Selection","issn_l":"0269-2139","issn":["0269-2139","1460-213X","1741-0126","1741-0134"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Engineering, Design and Selection","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086555916","display_name":"Abdel-Monem Abdalla","orcid":"https://orcid.org/0000-0003-2140-4891","observed_orcids":["https://orcid.org/0000-0003-2140-4891"]},"institutions":[{"id":"https://openalex.org/I123387679","display_name":"Uppsala University","ror":"https://ror.org/048a87296","country_code":"SE","type":"education","lineage":["https://openalex.org/I123387679"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Abdel-Monem Abdalla","raw_affiliation_strings":["Department of Biochemistry, Uppsala University, Biomedical Center, Box 576, SE-75123 Uppsala, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Uppsala University, Biomedical Center, Box 576, SE-75123 Uppsala, Sweden","institution_ids":["https://openalex.org/I123387679"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044615669","display_name":"Christopher M. Bruns","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I123387679","display_name":"Uppsala University","ror":"https://ror.org/048a87296","country_code":"SE","type":"education","lineage":["https://openalex.org/I123387679"]},{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["SE","US"],"is_corresponding":false,"raw_author_name":"Christopher M. Bruns","raw_affiliation_strings":["Scripps Research Institute","Uppsala University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Scripps Research Institute","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"Uppsala University","institution_ids":["https://openalex.org/I123387679"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059713279","display_name":"John A. Tainer","orcid":"https://orcid.org/0000-0003-1659-2429","observed_orcids":["https://orcid.org/0000-0003-1659-2429"]},"institutions":[{"id":"https://openalex.org/I123387679","display_name":"Uppsala University","ror":"https://ror.org/048a87296","country_code":"SE","type":"education","lineage":["https://openalex.org/I123387679"]},{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["SE","US"],"is_corresponding":false,"raw_author_name":"John A. Tainer","raw_affiliation_strings":["Scripps Research Institute","Uppsala University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Scripps Research Institute","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"Uppsala University","institution_ids":["https://openalex.org/I123387679"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080145082","display_name":"Bengt Mannervik","orcid":"https://orcid.org/0000-0002-6416-064X","observed_orcids":["https://orcid.org/0000-0002-6416-064X"]},"institutions":[{"id":"https://openalex.org/I123387679","display_name":"Uppsala University","ror":"https://ror.org/048a87296","country_code":"SE","type":"education","lineage":["https://openalex.org/I123387679"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Bengt Mannervik","raw_affiliation_strings":["Uppsala University ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Uppsala University ,","institution_ids":["https://openalex.org/I123387679"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062798141","display_name":"Gun Stenberg","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I123387679","display_name":"Uppsala University","ror":"https://ror.org/048a87296","country_code":"SE","type":"education","lineage":["https://openalex.org/I123387679"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Gun Stenberg","raw_affiliation_strings":["Uppsala University ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Uppsala University ,","institution_ids":["https://openalex.org/I123387679"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4656,"currency":"USD","value_usd":4656},"apc_paid":null,"fwci":0.4339,"has_fulltext":false,"cited_by_count":44,"citation_normalized_percentile":{"value":0.57486897,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"15","issue":"10","first_page":"827","last_page":"834"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12187","display_name":"Glutathione Transferases and Polymorphisms","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12187","display_name":"Glutathione Transferases and Polymorphisms","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.002199999988079071,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glutathione-s-transferase-pi","display_name":"glutathione S-transferase pi","score":0.8830000162124634},{"id":"https://openalex.org/keywords/gstp1","display_name":"GSTP1","score":0.9039999842643738},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.7879999876022339},{"id":"https://openalex.org/keywords/catalytic-inactivity","display_name":"catalytic inactivity","score":0.7850000262260437},{"id":"https://openalex.org/keywords/protein-stability","display_name":"protein stability","score":0.8610000014305115},{"id":"https://openalex.org/keywords/protein-redesign","display_name":"protein redesign","score":0.722000002861023},{"id":"https://openalex.org/keywords/in-vitro-evolution","display_name":"in vitro evolution","score":0.75}],"concepts":[{"id":"https://openalex.org/C166940927","wikidata":"https://www.wikidata.org/wiki/Q178827","display_name":"Monomer","level":3,"score":0.7719724178314209},{"id":"https://openalex.org/C133571119","wikidata":"https://www.wikidata.org/wiki/Q899102","display_name":"Circular dichroism","level":2,"score":0.6610535383224487},{"id":"https://openalex.org/C2779546866","wikidata":"https://www.wikidata.org/wiki/Q531306","display_name":"Dimer","level":2,"score":0.6522451043128967},{"id":"https://openalex.org/C2780122483","wikidata":"https://www.wikidata.org/wiki/Q18026593","display_name":"GSTP1","level":4,"score":0.6374602317810059},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6256136894226074},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5398925542831421},{"id":"https://openalex.org/C2776907368","wikidata":"https://www.wikidata.org/wiki/Q307302","display_name":"Glutathione S-transferase","level":4,"score":0.5354561805725098},{"id":"https://openalex.org/C2776376580","wikidata":"https://www.wikidata.org/wiki/Q407355","display_name":"Transferase","level":3,"score":0.47837033867836},{"id":"https://openalex.org/C538909803","wikidata":"https://www.wikidata.org/wiki/Q116907","display_name":"Glutathione","level":3,"score":0.4341072738170624},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.23976528644561768},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0738215446472168},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.06045535206794739}],"mesh":[{"descriptor_ui":"D000817","descriptor_name":"Anilino Naphthalenesulfonates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004137","descriptor_name":"Dinitrochlorobenzene","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005978","descriptor_name":"Glutathione","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D005978","descriptor_name":"Glutathione","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005982","descriptor_name":"Glutathione Transferase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005982","descriptor_name":"Glutathione Transferase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005982","descriptor_name":"Glutathione Transferase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019281","descriptor_name":"Dimerization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021122","descriptor_name":"Protein Subunits","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D021122","descriptor_name":"Protein Subunits","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D051549","descriptor_name":"Glutathione S-Transferase pi","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1093/protein/15.10.827","doi":"https://doi.org/10.1093/protein/15.10.827","is_oa":false,"landing_page_url":"https://doi.org/10.1093/protein/15.10.827","pdf_url":null,"source":{"id":"https://openalex.org/S44492648","display_name":"Protein Engineering Design and Selection","issn_l":"0269-2139","issn":["0269-2139","1460-213X","1741-0126","1741-0134"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Engineering, Design and Selection","raw_type":"journal-article"},{"id":"pmid:12468717","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12468717","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein engineering","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.1001.6194","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1001.6194","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://peds.oxfordjournals.org/content/15/10/827.full.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321501","display_name":"Carl Tryggers Stiftelse f\u00f6r Vetenskaplig Forskning","ror":"https://ror.org/048p4fn82"},{"id":"https://openalex.org/F4320322581","display_name":"Vetenskapsr\u00e5det","ror":"https://ror.org/03zttf063"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":42,"referenced_works":["https://openalex.org/W11118004","https://openalex.org/W80122595","https://openalex.org/W1544393038","https://openalex.org/W1581414347","https://openalex.org/W1773516573","https://openalex.org/W1787242221","https://openalex.org/W1842899761","https://openalex.org/W1853587765","https://openalex.org/W1902072415","https://openalex.org/W1965697145","https://openalex.org/W1974170352","https://openalex.org/W1983577170","https://openalex.org/W1998629716","https://openalex.org/W1998957960","https://openalex.org/W2010060878","https://openalex.org/W2018063944","https://openalex.org/W2018205221","https://openalex.org/W2020090957","https://openalex.org/W2026474027","https://openalex.org/W2037102375","https://openalex.org/W2037946624","https://openalex.org/W2042007689","https://openalex.org/W2046750894","https://openalex.org/W2061529389","https://openalex.org/W2066203136","https://openalex.org/W2068796470","https://openalex.org/W2074461690","https://openalex.org/W2079178286","https://openalex.org/W2079943300","https://openalex.org/W2080734821","https://openalex.org/W2090874882","https://openalex.org/W2098285182","https://openalex.org/W2100837269","https://openalex.org/W2110885671","https://openalex.org/W2111860128","https://openalex.org/W2128635872","https://openalex.org/W2138237526","https://openalex.org/W2150081412","https://openalex.org/W2153235080","https://openalex.org/W2160185798","https://openalex.org/W2324614614","https://openalex.org/W4238882428"],"related_works":["https://openalex.org/W7097323644","https://openalex.org/W2061529389","https://openalex.org/W584161923","https://openalex.org/W1996587462","https://openalex.org/W2080790710","https://openalex.org/W580574627","https://openalex.org/W1602857691","https://openalex.org/W4250637171","https://openalex.org/W376899492","https://openalex.org/W2070060505"],"abstract_inverted_index":{"By":[0],"the":[1,8,29,34,54,70,73,89],"introduction":[2],"of":[3,11,39,53,72,91],"10":[4],"site-specific":[5],"mutations":[6],"in":[7,83],"dimer":[9],"interface":[10],"human":[12],"glutathione":[13],"transferase":[14],"P1-1":[15],"(GSTP1-1),":[16],"a":[17,37,100],"stable":[18],"monomeric":[19,101],"protein":[20],"variant,":[21],"GSTP1,":[22],"was":[23],"obtained.":[24],"The":[25,76],"monomer":[26,55],"had":[27],"lost":[28],"catalytic":[30],"activity":[31],"but":[32],"retained":[33],"affinity":[35],"for":[36,46,82],"number":[38],"electrophilic":[40],"compounds":[41],"normally":[42],"serving":[43],"as":[44,80],"substrates":[45],"GSTP1-1.":[47],"Fluorescence":[48],"and":[49,56,86],"circular":[50],"dichroism":[51],"spectra":[52],"wild-type":[57],"proteins":[58,93],"were":[59],"similar,":[60],"indicating":[61],"that":[62],"there":[63],"are":[64],"no":[65],"large":[66],"structural":[67],"differences":[68],"between":[69],"subunits":[71],"respective":[74],"proteins.":[75],"GSTs":[77],"have":[78,105],"potential":[79],"targets":[81],"vitro":[84],"evolution":[85],"redesign":[87],"with":[88,94],"aim":[90],"developing":[92],"novel":[95],"properties.":[96],"To":[97],"this":[98],"end,":[99],"GST":[102],"variant":[103],"may":[104],"distinct":[106],"advantages.":[107]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
