{"id":"https://openalex.org/W2097410907","doi":null,"title":"Hydrogen/deuterium-exchange (DXMS) analysis of the carbohydrate phosphatase, starch-excess 4","display_name":"Hydrogen/deuterium-exchange (DXMS) analysis of the carbohydrate phosphatase, starch-excess 4","publication_year":2008,"publication_date":"2008-01-01","ids":{"openalex":"https://openalex.org/W2097410907","mag":"2097410907"},"language":"en","primary_location":{"id":"pmh:oai:escholarship.org/ark:/13030/qt3bp082q0","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/3bp082q0","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5025563270","display_name":"Simon Hsu","orcid":"https://orcid.org/0000-0003-3780-294X","observed_orcids":["https://orcid.org/0000-0003-3780-294X"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Simon Hsu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5025563270"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12604","display_name":"Glycogen Storage Diseases and Myoclonus","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12604","display_name":"Glycogen Storage Diseases and Myoclonus","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/laforin","display_name":"laforin","score":0.9470000267028809},{"id":"https://openalex.org/keywords/lafora-disease","display_name":"Lafora disease","score":0.9649999737739563},{"id":"https://openalex.org/keywords/carbohydrate-binding-module","display_name":"carbohydrate-binding module","score":0.9089999794960022},{"id":"https://openalex.org/keywords/phosphatase-domain","display_name":"phosphatase domain","score":0.8309999704360962},{"id":"https://openalex.org/keywords/solvent-accessibility","display_name":"solvent accessibility","score":0.7730000019073486},{"id":"https://openalex.org/keywords/substrate-induced-conformational-change","display_name":"substrate-induced conformational change","score":0.9049999713897705},{"id":"https://openalex.org/keywords/allosteric-regulation","display_name":"allosteric regulation","score":0.9269999861717224},{"id":"https://openalex.org/keywords/protein-structural-dynamics","display_name":"protein structural dynamics","score":0.75}],"concepts":[{"id":"https://openalex.org/C2777280662","wikidata":"https://www.wikidata.org/wiki/Q163905","display_name":"Lafora disease","level":4,"score":0.7042554616928101},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6760188341140747},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6156153678894043},{"id":"https://openalex.org/C167569220","wikidata":"https://www.wikidata.org/wiki/Q5954985","display_name":"Hydrogen\u2013deuterium exchange","level":3,"score":0.6117329001426697},{"id":"https://openalex.org/C178666793","wikidata":"https://www.wikidata.org/wiki/Q422476","display_name":"Phosphatase","level":3,"score":0.6101400256156921},{"id":"https://openalex.org/C166342909","wikidata":"https://www.wikidata.org/wiki/Q845326","display_name":"Allosteric regulation","level":3,"score":0.4529995322227478},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.43351441621780396},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.34324413537979126},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2713667154312134},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2487889528274536},{"id":"https://openalex.org/C512968161","wikidata":"https://www.wikidata.org/wiki/Q556","display_name":"Hydrogen","level":2,"score":0.07693710923194885},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:escholarship.org/ark:/13030/qt3bp082q0","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/3bp082q0","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},{"id":"mag:2097410907","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/content/qt3bp082q0/qt3bp082q0.pdf?t=lq4vn2","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W351514936","https://openalex.org/W1966603445","https://openalex.org/W1968718594","https://openalex.org/W1969024152","https://openalex.org/W1977416006","https://openalex.org/W1977581525","https://openalex.org/W1977749455","https://openalex.org/W1983502971","https://openalex.org/W1987453674","https://openalex.org/W1988966516","https://openalex.org/W1989899886","https://openalex.org/W1990126142","https://openalex.org/W2012451447","https://openalex.org/W2019573907","https://openalex.org/W2021182257","https://openalex.org/W2027815803","https://openalex.org/W2033882290","https://openalex.org/W2038132994","https://openalex.org/W2043634254","https://openalex.org/W2047584139","https://openalex.org/W2049852307","https://openalex.org/W2053200073","https://openalex.org/W2053839044","https://openalex.org/W2068427129","https://openalex.org/W2072730326","https://openalex.org/W2073863845","https://openalex.org/W2076337632","https://openalex.org/W2091000925","https://openalex.org/W2093262143","https://openalex.org/W2109699278","https://openalex.org/W2114320763","https://openalex.org/W2125936461","https://openalex.org/W2135287247","https://openalex.org/W2141196514","https://openalex.org/W2154404663","https://openalex.org/W2181285684","https://openalex.org/W2414384112","https://openalex.org/W2416542900","https://openalex.org/W2439984939"],"related_works":["https://openalex.org/W1491809435","https://openalex.org/W2287220089","https://openalex.org/W1977024641","https://openalex.org/W2299716070","https://openalex.org/W2109699278","https://openalex.org/W3174356094","https://openalex.org/W2167598880","https://openalex.org/W2088859286","https://openalex.org/W7097349480","https://openalex.org/W2038132994"],"abstract_inverted_index":{"Lafora":[0,28],"Disease":[1],"(LD),":[2],"a":[3,10,62,67,164,205,216],"type":[4],"of":[5,17,22,40,99,109,117,122,144,155,162,170,185,207,229,237,244,264],"progressive":[6],"myoclonus":[7],"epilepsy,":[8],"is":[9,19],"fatal":[11],"autosomal":[12],"recessive":[13],"disorder.":[14],"The":[15,227,261],"hallmark":[16],"LD":[18,42],"the":[20,49,53,96,100,107,118,141,156,160,186,194,208,241],"accumulation":[21],"insoluble":[23],"polyglucosan":[24],"deposits":[25],"known":[26,168],"as":[27],"bodies":[29],"in":[30,48,137,151,183,193,204,212,233],"nerve,":[31],"heart,":[32],"liver,":[33],"skin":[34],"and":[35,66,76,95,167,173,192,253,258],"skeletal":[36],"muscle":[37],"cells.":[38],"Half":[39],"all":[41,234],"cases":[43],"are":[44],"caused":[45],"by":[46],"mutations":[47],"gene":[50],"that":[51,240],"encodes":[52],"dual":[54],"specificity":[55],"phosphatase,":[56],"laforin.":[57],"Laforin":[58],"contains":[59],"two":[60,101],"domains:":[61],"carbohydrate-binding":[63,225],"module":[64],"(CBM)":[65],"phosphatase":[68,195,242],"domain.":[69],"Together,":[70],"they":[71],"allow":[72],"laforin":[73,88,110,172,267,277],"to":[74,85,91,106,139],"bind":[75],"dephosphorylate":[77],"complex":[78],"carbohydrates,":[79],"an":[80,256],"activity":[81],"conserved":[82,189],"from":[83],"humans":[84],"plants.":[86],"However,":[87],"has":[89],"yet":[90,223],"be":[92],"crystallized":[93],"successfully,":[94],"dynamic":[97],"behavior":[98],"domains":[102],"remains":[103],"unknown.":[104],"Due":[105],"unavailability":[108],"for":[111,276],"study,":[112],"we":[113,177],"probed":[114],"solvent":[115],"accessibility":[116],"plant":[119],"functional":[120,262],"equivalent":[121],"laforin,":[123],"starch-excess":[124],"4":[125],"(SEX4),":[126],"utilizing":[127],"enhanced":[128],"peptide":[129],"amide":[130],"hydrogen/deuterium":[131],"(H/D)":[132],"exchange":[133,154,181,230],"mass":[134],"spectrometry":[135],"(DXMS)":[136],"order":[138],"elucidate":[140],"structural":[142],"dynamics":[143],"these":[145],"proteins.":[146],"We":[147],"also":[148,202],"explored":[149],"changes":[150,221,232,249],"H/":[152],"D":[153],"SEX4":[157,238,245,265],"protein":[158],"upon":[159,250],"binding":[161,190],"amylopectin,":[163],"glucose":[165],"polymer":[166],"substrate":[169,251],"both":[171],"SEX4.":[174],"Employing":[175],"DXMS,":[176],"observed":[178,203],"substrate-":[179],"induced":[180],"decrease":[182,200],"regions":[184,236],"CBM":[187,209],"with":[188,266],"residues":[191],"active":[196,259],"site":[197,217],"pocket.":[198],"Exchange":[199],"was":[201],"loop":[206],"not":[210],"implicated":[211],"carbohydrate":[213],"binding,":[214,252],"indicating":[215],"undergoing":[218],"substrate-induced":[219],"allosteric":[220],"or":[222],"unidentified":[224],"residues.":[226],"lack":[228],"rate":[231],"other":[235],"indicates":[239],"domain":[243],"undergoes":[246],"little":[247],"conformational":[248],"likely":[254,271],"retains":[255],"open":[257],"conformation.":[260],"homology":[263],"makes":[268],"it":[269],"highly":[270],"our":[272],"results":[273],"hold":[274],"true":[275]},"counts_by_year":[{"year":2013,"cited_by_count":1}],"updated_date":"2026-08-18T07:49:30.821534","created_date":"2016-06-24T00:00:00"}