{"id":"https://openalex.org/W2097146341","doi":null,"title":"ENZYME IMMOBILIZATION INTO POLYMERS AND COATINGS","display_name":"ENZYME IMMOBILIZATION INTO POLYMERS AND COATINGS","publication_year":2002,"publication_date":"2002-12-02","ids":{"openalex":"https://openalex.org/W2097146341","mag":"2097146341"},"language":"en","primary_location":{"id":"pmh:oai:d-scholarship.pitt.edu:9500","doi":null,"is_oa":true,"landing_page_url":"https://d-scholarship.pitt.edu/9500/1/etd_drevongf.pdf","pdf_url":"http://d-scholarship.pitt.edu/9500/1/etd_drevongf.pdf","source":{"id":"https://openalex.org/S4306402372","display_name":"D-Scholarship@Pitt (University of Pittsburgh)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I170201317","host_organization_name":"University of Pittsburgh","host_organization_lineage":["https://openalex.org/I170201317"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"PeerReviewed"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://d-scholarship.pitt.edu/9500/1/etd_drevongf.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015591485","display_name":"G\u00e9raldine F. Drevon","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"G\u00e9raldine F. Drevon","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5015591485"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":8,"citation_normalized_percentile":{"value":0.12830728,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":95},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10343","display_name":"Hydrogels: synthesis, properties, applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/enzyme-immobilization","display_name":"enzyme immobilization","score":0.9980000257492065},{"id":"https://openalex.org/keywords/polyurethane-foam","display_name":"polyurethane foam","score":0.8700000047683716},{"id":"https://openalex.org/keywords/bioplastics","display_name":"bioplastics","score":0.7049999833106995},{"id":"https://openalex.org/keywords/enzyme-coating","display_name":"enzyme coating","score":0.609000027179718},{"id":"https://openalex.org/keywords/atom-transfer-radical-polymerization","display_name":"atom transfer radical polymerization","score":0.8920000195503235},{"id":"https://openalex.org/keywords/enzyme-thermostability","display_name":"enzyme thermostability","score":0.6909999847412109},{"id":"https://openalex.org/keywords/enzyme-deactivation-kinetics","display_name":"enzyme deactivation kinetics","score":0.8690000176429749},{"id":"https://openalex.org/keywords/carbonic-anhydrase","display_name":"carbonic anhydrase","score":0.9340000152587891},{"id":"https://openalex.org/keywords/enzyme-activity-retention","display_name":"enzyme activity retention","score":0.5440000295639038},{"id":"https://openalex.org/keywords/water-soluble-polymers","display_name":"water-soluble polymers","score":0.6589999794960022}],"concepts":[{"id":"https://openalex.org/C43719734","wikidata":"https://www.wikidata.org/wiki/Q413458","display_name":"Bioplastic","level":2,"score":0.778027355670929},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.6354928612709045},{"id":"https://openalex.org/C33633552","wikidata":"https://www.wikidata.org/wiki/Q904145","display_name":"Immobilized enzyme","level":3,"score":0.6045553088188171},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.600969135761261},{"id":"https://openalex.org/C188027245","wikidata":"https://www.wikidata.org/wiki/Q750446","display_name":"Polymer chemistry","level":1,"score":0.5999411940574646},{"id":"https://openalex.org/C2781216036","wikidata":"https://www.wikidata.org/wiki/Q902494","display_name":"Thermostability","level":3,"score":0.5955506563186646},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.5667375922203064},{"id":"https://openalex.org/C150539798","wikidata":"https://www.wikidata.org/wiki/Q757561","display_name":"Atom-transfer radical-polymerization","level":4,"score":0.5327123999595642},{"id":"https://openalex.org/C166940927","wikidata":"https://www.wikidata.org/wiki/Q178827","display_name":"Monomer","level":3,"score":0.4886679947376251},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.4829546809196472},{"id":"https://openalex.org/C44228677","wikidata":"https://www.wikidata.org/wiki/Q181898","display_name":"Polymerization","level":3,"score":0.4793725311756134},{"id":"https://openalex.org/C2779578285","wikidata":"https://www.wikidata.org/wiki/Q146322","display_name":"Polyurethane","level":2,"score":0.4305265545845032},{"id":"https://openalex.org/C2781448156","wikidata":"https://www.wikidata.org/wiki/Q1570182","display_name":"Coating","level":2,"score":0.42046689987182617},{"id":"https://openalex.org/C42360764","wikidata":"https://www.wikidata.org/wiki/Q83588","display_name":"Chemical engineering","level":1,"score":0.372835636138916},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.34556883573532104},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3136788010597229},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:d-scholarship.pitt.edu:9500","doi":null,"is_oa":true,"landing_page_url":"https://d-scholarship.pitt.edu/9500/1/etd_drevongf.pdf","pdf_url":"http://d-scholarship.pitt.edu/9500/1/etd_drevongf.pdf","source":{"id":"https://openalex.org/S4306402372","display_name":"D-Scholarship@Pitt (University of Pittsburgh)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I170201317","host_organization_name":"University of Pittsburgh","host_organization_lineage":["https://openalex.org/I170201317"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"PeerReviewed"},{"id":"mag:2097146341","doi":null,"is_oa":false,"landing_page_url":"http://d-scholarship.pitt.edu/9500/1/etd_drevongf.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:d-scholarship.pitt.edu:9500","doi":null,"is_oa":true,"landing_page_url":"https://d-scholarship.pitt.edu/9500/1/etd_drevongf.pdf","pdf_url":"http://d-scholarship.pitt.edu/9500/1/etd_drevongf.pdf","source":{"id":"https://openalex.org/S4306402372","display_name":"D-Scholarship@Pitt (University of Pittsburgh)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I170201317","host_organization_name":"University of Pittsburgh","host_organization_lineage":["https://openalex.org/I170201317"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"PeerReviewed"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2097146341.pdf","grobid_xml":"https://content.openalex.org/works/W2097146341.grobid-xml"},"referenced_works_count":218,"referenced_works":["https://openalex.org/W67572188","https://openalex.org/W103118658","https://openalex.org/W112888018","https://openalex.org/W133195234","https://openalex.org/W160207307","https://openalex.org/W183034448","https://openalex.org/W192973662","https://openalex.org/W198999690","https://openalex.org/W606498232","https://openalex.org/W1005834122","https://openalex.org/W1028329593","https://openalex.org/W1194915374","https://openalex.org/W1490786921","https://openalex.org/W1500041659","https://openalex.org/W1508017329","https://openalex.org/W1513720422","https://openalex.org/W1530704185","https://openalex.org/W1531861593","https://openalex.org/W1542029403","https://openalex.org/W1549240509","https://openalex.org/W1574324925","https://openalex.org/W1576632530","https://openalex.org/W1585079099","https://openalex.org/W1591166439","https://openalex.org/W1592741796","https://openalex.org/W1593567129","https://openalex.org/W1604752385","https://openalex.org/W1619383772","https://openalex.org/W1769022385","https://openalex.org/W1845516098","https://openalex.org/W1898812238","https://openalex.org/W1963559630","https://openalex.org/W1970113653","https://openalex.org/W1971613252","https://openalex.org/W1972214979","https://openalex.org/W1972569526","https://openalex.org/W1973866035","https://openalex.org/W1974889352","https://openalex.org/W1975410474","https://openalex.org/W1975890957","https://openalex.org/W1976323123","https://openalex.org/W1976731830","https://openalex.org/W1977419315","https://openalex.org/W1977545144","https://openalex.org/W1978257444","https://openalex.org/W1978446047","https://openalex.org/W1980337066","https://openalex.org/W1981064297","https://openalex.org/W1981139153","https://openalex.org/W1981878997","https://openalex.org/W1982194116","https://openalex.org/W1983520034","https://openalex.org/W1983931091","https://openalex.org/W1984443502","https://openalex.org/W1984595761","https://openalex.org/W1984659752","https://openalex.org/W1984809314","https://openalex.org/W1985470384","https://openalex.org/W1986830687","https://openalex.org/W1987410104","https://openalex.org/W1988579434","https://openalex.org/W1989404987","https://openalex.org/W1991957962","https://openalex.org/W1993063222","https://openalex.org/W1993231572","https://openalex.org/W1995080813","https://openalex.org/W1995204544","https://openalex.org/W1996014236","https://openalex.org/W1996097047","https://openalex.org/W1999167281","https://openalex.org/W1999629537","https://openalex.org/W1999935633","https://openalex.org/W2002099469","https://openalex.org/W2002271027","https://openalex.org/W2004434392","https://openalex.org/W2004686422","https://openalex.org/W2004773817","https://openalex.org/W2006432342","https://openalex.org/W2007209273","https://openalex.org/W2007256142","https://openalex.org/W2007673201","https://openalex.org/W2008457986","https://openalex.org/W2008471702","https://openalex.org/W2009631702","https://openalex.org/W2011870243","https://openalex.org/W2012333204","https://openalex.org/W2013289084","https://openalex.org/W2014396096","https://openalex.org/W2014910955","https://openalex.org/W2014963621","https://openalex.org/W2015139372","https://openalex.org/W2017888522","https://openalex.org/W2018232164","https://openalex.org/W2019251201","https://openalex.org/W2021977745","https://openalex.org/W2022510326","https://openalex.org/W2022537484","https://openalex.org/W2023217057","https://openalex.org/W2023602117","https://openalex.org/W2025377293","https://openalex.org/W2026195929","https://openalex.org/W2026244387","https://openalex.org/W2027064015","https://openalex.org/W2027766347","https://openalex.org/W2027925207","https://openalex.org/W2028143683","https://openalex.org/W2028744855","https://openalex.org/W2030256371","https://openalex.org/W2030686225","https://openalex.org/W2032687084","https://openalex.org/W2034020541","https://openalex.org/W2034712246","https://openalex.org/W2035012882","https://openalex.org/W2035046825","https://openalex.org/W2035146203","https://openalex.org/W2037550000","https://openalex.org/W2037819309","https://openalex.org/W2037848855","https://openalex.org/W2038365960","https://openalex.org/W2038447808","https://openalex.org/W2038869574","https://openalex.org/W2039382734","https://openalex.org/W2040229582","https://openalex.org/W2040409983","https://openalex.org/W2041036003","https://openalex.org/W2041705038","https://openalex.org/W2041785888","https://openalex.org/W2042161144","https://openalex.org/W2045055557","https://openalex.org/W2047357983","https://openalex.org/W2048325133","https://openalex.org/W2049013168","https://openalex.org/W2049920069","https://openalex.org/W2051961784","https://openalex.org/W2052520834","https://openalex.org/W2053921551","https://openalex.org/W2057790212","https://openalex.org/W2057878564","https://openalex.org/W2059024399","https://openalex.org/W2060194574","https://openalex.org/W2060435488","https://openalex.org/W2061130991","https://openalex.org/W2062210965","https://openalex.org/W2063302509","https://openalex.org/W2063686739","https://openalex.org/W2064175091","https://openalex.org/W2064814612","https://openalex.org/W2064824818","https://openalex.org/W2065609255","https://openalex.org/W2066661817","https://openalex.org/W2067541220","https://openalex.org/W2067807344","https://openalex.org/W2069508413","https://openalex.org/W2069543322","https://openalex.org/W2071086763","https://openalex.org/W2071098592","https://openalex.org/W2074677946","https://openalex.org/W2075076617","https://openalex.org/W2076143662","https://openalex.org/W2076631959","https://openalex.org/W2077118080","https://openalex.org/W2078243332","https://openalex.org/W2078307829","https://openalex.org/W2078824773","https://openalex.org/W2083034341","https://openalex.org/W2083686106","https://openalex.org/W2083868665","https://openalex.org/W2084317732","https://openalex.org/W2085096699","https://openalex.org/W2088319126","https://openalex.org/W2088862173","https://openalex.org/W2089104112","https://openalex.org/W2090244087","https://openalex.org/W2091665035","https://openalex.org/W2091731207","https://openalex.org/W2091895931","https://openalex.org/W2092403165","https://openalex.org/W2093248733","https://openalex.org/W2094908737","https://openalex.org/W2098807743","https://openalex.org/W2101101710","https://openalex.org/W2104517032","https://openalex.org/W2108849916","https://openalex.org/W2109058950","https://openalex.org/W2113502101","https://openalex.org/W2122486223","https://openalex.org/W2123093625","https://openalex.org/W2124367513","https://openalex.org/W2127840001","https://openalex.org/W2128635872","https://openalex.org/W2131478412","https://openalex.org/W2131923865","https://openalex.org/W2133393350","https://openalex.org/W2134060539","https://openalex.org/W2139382689","https://openalex.org/W2143315188","https://openalex.org/W2144120263","https://openalex.org/W2148379559","https://openalex.org/W2151045960","https://openalex.org/W2151475317","https://openalex.org/W2151997301","https://openalex.org/W2156470306","https://openalex.org/W2159123606","https://openalex.org/W2159422614","https://openalex.org/W2160437831","https://openalex.org/W2405066143","https://openalex.org/W2410484433","https://openalex.org/W2464076451","https://openalex.org/W2469672798","https://openalex.org/W2485774056","https://openalex.org/W2494244385","https://openalex.org/W2496977291","https://openalex.org/W2511191756","https://openalex.org/W2515230585","https://openalex.org/W2952217440","https://openalex.org/W3204251758","https://openalex.org/W4231261872","https://openalex.org/W4251700885"],"related_works":["https://openalex.org/W2025032111","https://openalex.org/W2026403516","https://openalex.org/W4232402406","https://openalex.org/W1984877957","https://openalex.org/W2470198519","https://openalex.org/W2069543322","https://openalex.org/W2075076617","https://openalex.org/W1976968610","https://openalex.org/W2146905617","https://openalex.org/W4408257079"],"abstract_inverted_index":{"In":[0],"this":[1,134],"study,":[2],"we":[3],"have":[4],"developed":[5],"strategies":[6],"to":[7,43,84,155,212],"immobilize":[8],"enzymes":[9],"into":[10,34,168,240,251],"various":[11],"polymer":[12,131,243],"and":[13,90,118,125,149,272],"coatings.":[14,170],"Three":[15],"categories":[16],"of":[17,25,46,54,80,87,94,102,128,159,173,214,219,254,279],"bioplastic":[18,40,215],"matrices":[19],"were":[20,107,181],"investigated.":[21,163],"The":[22,38,52,171,186,217,245,257],"first":[23,237],"type":[24],"bioplastics":[26,160],"was":[27,56,136,144,162,165,223,236,248],"prepared":[28,109],"by":[29,59],"irreversibly":[30,166],"incorporating":[31],"di-isopropylfluorophosphatase":[32],"(DFPase)":[33],"polyurethane":[35],"(PU)":[36],"foams.":[37,216],"resulting":[39,187,246],"retained":[41,273],"up":[42],"67":[44],"%":[45],"the":[47,60,76,85,103,123,156,184,252],"activity":[48,142,179,275],"for":[49],"native":[50,64],"enzyme.":[51,95],"thermostability":[53],"DFPase":[55,67,82,164,175],"highly":[57],"affected":[58],"immobilization":[61,127],"process.":[62],"Unlike":[63],"enzyme,":[65],"immobilized":[66,81,174,239],"had":[68,206],"biphasic":[69,208],"deactivation":[70,79,209],"kinetics.":[71],"Our":[72],"data":[73],"demonstrated":[74],"that":[75,161,213],"initial":[77],"rapid":[78],"lead":[83],"formation":[86],"a":[88,99,207,226],"hyper-stable":[89,104],"still":[91],"active":[92],"form":[93],"Spectroscopic":[96],"studies":[97],"enabled":[98],"structural":[100],"analysis":[101],"intermediate.":[105],"Biopolymers":[106],"also":[108],"via":[110],"atom":[111],"transfer":[112],"radical":[113],"polymerization":[114],"(ATRP)":[115],"using":[116,229],"acrylic":[117],"sulfonate-derived":[119],"monomers.":[120],"ATRP":[121],"ensured":[122],"covalent":[124],"multi-point":[126],"enzyme":[129],"within":[130,183],"matrices.":[132],"However,":[133],"approach":[135],"only":[137],"partially":[138],"successful,":[139],"as":[140,176,178],"no":[141],"retention":[143,180],"obtained":[145],"after":[146,276],"polymerizationEnzyme-containing":[147],"PU-":[148],"Michael":[150],"adduct":[151],"(MA)-based":[152],"coatings":[153,222],"correspond":[154],"last":[157],"category":[158],"incorporated":[167],"PU":[169],"distribution":[172],"well":[177],"homogeneous":[182],"coating.":[185,256],"enzyme-containing":[188,220],"coating":[189],"(ECC)":[190],"film":[191],"hydrolyzed":[192],"DFP":[193],"in":[194,225],"buffered":[195],"media":[196],"at":[197],"high":[198],"rates":[199],"retaining":[200],"approximately":[201,261],"39%":[202],"intrinsic":[203],"activity.":[204,264],"DFPase-ECC":[205],"profile":[210],"similar":[211],"synthesis":[218],"MA":[221,255],"performed":[224],"two-step":[227],"process":[228],"carbonic":[230],"anhydrase":[231],"(CA,":[232],"E.C.":[233],"4.2.1.1).":[234],"CA":[235],"covalently":[238],"NVF-based":[241],"water-soluble":[242],"(EP).":[244],"EP":[247],"further":[249],"entrapped":[250],"matrix":[253],"so-formed":[258],"ECC's":[259],"exhibited":[260],"7%":[262],"apparent":[263],"CA-ECC":[265],"showed":[266],"good":[267],"stability":[268],"under":[269],"ambient":[270],"conditions":[271],"55%":[274],"90":[277],"days":[278],"storage.":[280]},"counts_by_year":[{"year":2015,"cited_by_count":2}],"updated_date":"2026-10-27T15:51:10.671819","created_date":"2016-06-24T00:00:00"}