{"id":"https://openalex.org/W2097054866","doi":"https://doi.org/10.1089/15406580360545107","title":"High Throughput Screening Technologies for Direct Cyclic AMP Measurement","display_name":"High Throughput Screening Technologies for Direct Cyclic AMP Measurement","publication_year":2003,"publication_date":"2003-04-01","ids":{"openalex":"https://openalex.org/W2097054866","doi":"https://doi.org/10.1089/15406580360545107","mag":"2097054866","pmid":"https://pubmed.ncbi.nlm.nih.gov/15090194"},"language":"en","primary_location":{"id":"doi:10.1089/15406580360545107","doi":"https://doi.org/10.1089/15406580360545107","is_oa":false,"landing_page_url":"https://doi.org/10.1089/15406580360545107","pdf_url":null,"source":{"id":"https://openalex.org/S76283439","display_name":"Assay and Drug Development Technologies","issn_l":"1540-658X","issn":["1540-658X","1557-8127"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ASSAY and Drug Development Technologies","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5038124225","display_name":"Daniela Gabriel","orcid":"https://orcid.org/0000-0002-0362-6274","observed_orcids":["https://orcid.org/0000-0002-0362-6274"]},"institutions":[{"id":"https://openalex.org/I1283582996","display_name":"Novartis (Switzerland)","ror":"https://ror.org/02f9zrr09","country_code":"CH","type":"company","lineage":["https://openalex.org/I1283582996"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Daniela Gabriel","raw_affiliation_strings":["Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland","Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland. daniela.gabriel@pharma.novartis.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland","institution_ids":["https://openalex.org/I1283582996"]},{"raw_affiliation_string":"Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland. daniela.gabriel@pharma.novartis.com","institution_ids":["https://openalex.org/I1283582996"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091051452","display_name":"Magali Vernier","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1283582996","display_name":"Novartis (Switzerland)","ror":"https://ror.org/02f9zrr09","country_code":"CH","type":"company","lineage":["https://openalex.org/I1283582996"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Magali Vernier","raw_affiliation_strings":["Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland","institution_ids":["https://openalex.org/I1283582996"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113696177","display_name":"Martin Pfeifer","orcid":"https://orcid.org/0000-0001-9126-5685","observed_orcids":["https://orcid.org/0000-0001-9126-5685"]},"institutions":[{"id":"https://openalex.org/I1283582996","display_name":"Novartis (Switzerland)","ror":"https://ror.org/02f9zrr09","country_code":"CH","type":"company","lineage":["https://openalex.org/I1283582996"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Martin J. Pfeifer","raw_affiliation_strings":["Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland","institution_ids":["https://openalex.org/I1283582996"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024917578","display_name":"Boris Dasen","orcid":"https://orcid.org/0009-0003-1638-3253","observed_orcids":["https://orcid.org/0009-0003-1638-3253"]},"institutions":[{"id":"https://openalex.org/I1283582996","display_name":"Novartis (Switzerland)","ror":"https://ror.org/02f9zrr09","country_code":"CH","type":"company","lineage":["https://openalex.org/I1283582996"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Boris Dasen","raw_affiliation_strings":["Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland","institution_ids":["https://openalex.org/I1283582996"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084761712","display_name":"Laurent Tenaillon","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1283582996","display_name":"Novartis (Switzerland)","ror":"https://ror.org/02f9zrr09","country_code":"CH","type":"company","lineage":["https://openalex.org/I1283582996"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Laurent Tenaillon","raw_affiliation_strings":["Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland","institution_ids":["https://openalex.org/I1283582996"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5025503202","display_name":"Rochdi Bouhelal","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1283582996","display_name":"Novartis (Switzerland)","ror":"https://ror.org/02f9zrr09","country_code":"CH","type":"company","lineage":["https://openalex.org/I1283582996"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Rochdi Bouhelal","raw_affiliation_strings":["Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Novartis Pharma AG, Lead Discovery Center, Basel, Switzerland","institution_ids":["https://openalex.org/I1283582996"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1283582996"],"apc_list":null,"apc_paid":null,"fwci":1.2257,"has_fulltext":false,"cited_by_count":136,"citation_normalized_percentile":{"value":0.76194992,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"1","issue":"2","first_page":"291","last_page":"303"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12434","display_name":"Phosphodiesterase function and regulation","score":0.41110000014305115,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12434","display_name":"Phosphodiesterase function and regulation","score":0.41110000014305115,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.3082999885082245,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.1404000073671341,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.9020000100135803},{"id":"https://openalex.org/keywords/delfia","display_name":"DELFIA","score":0.781000018119812},{"id":"https://openalex.org/keywords/tr-fret","display_name":"time-resolved fluorescence resonance energy transfer","score":0.8019999861717224},{"id":"https://openalex.org/keywords/alphascreen","display_name":"AlphaScreen","score":0.7670000195503235},{"id":"https://openalex.org/keywords/fluorescence-polarization","display_name":"fluorescence polarization","score":0.6549999713897705},{"id":"https://openalex.org/keywords/enzyme-complementation","display_name":"enzyme complementation","score":0.8650000095367432},{"id":"https://openalex.org/keywords/g-protein-coupled-receptor-antagonists","display_name":"G protein-coupled receptor antagonists","score":0.8679999709129333},{"id":"https://openalex.org/keywords/signal-sensitivity","display_name":"signal sensitivity","score":0.39399999380111694},{"id":"https://openalex.org/keywords/dynamic-range","display_name":"dynamic range","score":0.546999990940094}],"concepts":[{"id":"https://openalex.org/C63479239","wikidata":"https://www.wikidata.org/wiki/Q7353546","display_name":"Robustness (evolution)","level":3,"score":0.5733882188796997},{"id":"https://openalex.org/C2777516457","wikidata":"https://www.wikidata.org/wiki/Q2413613","display_name":"Singlet oxygen","level":3,"score":0.5593685507774353},{"id":"https://openalex.org/C51323132","wikidata":"https://www.wikidata.org/wiki/Q626251","display_name":"High-throughput screening","level":2,"score":0.5508707165718079},{"id":"https://openalex.org/C21200559","wikidata":"https://www.wikidata.org/wiki/Q7451068","display_name":"Sensitivity (control systems)","level":2,"score":0.5067904591560364},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.4650382399559021},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.44230690598487854},{"id":"https://openalex.org/C66882249","wikidata":"https://www.wikidata.org/wiki/Q169336","display_name":"Homogeneous","level":2,"score":0.4364963173866272},{"id":"https://openalex.org/C40875361","wikidata":"https://www.wikidata.org/wiki/Q2356542","display_name":"Reagent","level":2,"score":0.4330741763114929},{"id":"https://openalex.org/C148869448","wikidata":"https://www.wikidata.org/wiki/Q184240","display_name":"Luminescence","level":2,"score":0.43033039569854736},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3323379158973694},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.2886693477630615},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19084712862968445},{"id":"https://openalex.org/C49040817","wikidata":"https://www.wikidata.org/wiki/Q193091","display_name":"Optoelectronics","level":1,"score":0.18008723855018616},{"id":"https://openalex.org/C540031477","wikidata":"https://www.wikidata.org/wiki/Q629","display_name":"Oxygen","level":2,"score":0.13996386528015137},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.07221657037734985},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.0},{"id":"https://openalex.org/C24326235","wikidata":"https://www.wikidata.org/wiki/Q126095","display_name":"Electronic engineering","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000242","descriptor_name":"Cyclic AMP","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D000242","descriptor_name":"Cyclic AMP","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001681","descriptor_name":"Biological Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D005453","descriptor_name":"Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005576","descriptor_name":"Colforsin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008163","descriptor_name":"Luminescent Measurements","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011933","descriptor_name":"Reagent Kits, Diagnostic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013678","descriptor_name":"Technology, Pharmaceutical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015200","descriptor_name":"Fluoroimmunoassay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019204","descriptor_name":"GTP-Binding Proteins","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D019204","descriptor_name":"GTP-Binding Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019204","descriptor_name":"GTP-Binding Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1089/15406580360545107","doi":"https://doi.org/10.1089/15406580360545107","is_oa":false,"landing_page_url":"https://doi.org/10.1089/15406580360545107","pdf_url":null,"source":{"id":"https://openalex.org/S76283439","display_name":"Assay and Drug Development Technologies","issn_l":"1540-658X","issn":["1540-658X","1557-8127"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ASSAY and Drug Development Technologies","raw_type":"journal-article"},{"id":"pmid:15090194","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15090194","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Assay and drug development technologies","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W1482181189","https://openalex.org/W1965960192","https://openalex.org/W1966903992","https://openalex.org/W1991001144","https://openalex.org/W1996437478","https://openalex.org/W2016086077","https://openalex.org/W2026799822","https://openalex.org/W2029611470","https://openalex.org/W2042750495","https://openalex.org/W2042950013","https://openalex.org/W2046425717","https://openalex.org/W2046736685","https://openalex.org/W2052227833","https://openalex.org/W2052950503","https://openalex.org/W2080106148","https://openalex.org/W2081626878","https://openalex.org/W2082908197","https://openalex.org/W2100765179","https://openalex.org/W2123061071","https://openalex.org/W2128375888","https://openalex.org/W2141216709","https://openalex.org/W2150424983","https://openalex.org/W2153685729","https://openalex.org/W2154946507","https://openalex.org/W2914936981"],"related_works":["https://openalex.org/W2039654095","https://openalex.org/W1991718269","https://openalex.org/W2376434189","https://openalex.org/W4249908221","https://openalex.org/W2100146331","https://openalex.org/W7097392588","https://openalex.org/W2063205376","https://openalex.org/W2032911782","https://openalex.org/W2142959204","https://openalex.org/W7143402199"],"abstract_inverted_index":{"A":[0],"study":[1],"comparing":[2],"five":[3],"different":[4],"cAMP":[5],"detection":[6],"technologies":[7],"in":[8],"terms":[9],"of":[10],"sensitivity,":[11,54,64],"robustness,":[12],"and":[13,30,46,57,68,80,85],"feasibility":[14],"for":[15],"HTS":[16],"is":[17],"presented.":[18],"In":[19],"this":[20],"report,":[21],"the":[22,31,52,82],"following":[23],"methods":[24,33],"are":[25],"described:":[26],"a":[27],"nonhomogeneous":[28],"DELFIA,":[29],"homogeneous":[32],"based":[34],"on":[35],"time-resolved":[36],"fluorescence":[37],"(HTRF),":[38],"luminescent":[39],"singlet":[40],"oxygen":[41],"channeling":[42],"or":[43],"ALPHAScreen,":[44],"FP,":[45],"high-affinity":[47],"enzyme":[48],"complementation.":[49],"DELFIA":[50],"had":[51],"highest":[53],"whereas":[55],"ALPHAScreen":[56,81],"HTRF":[58,79],"shared":[59],"several":[60],"advantages,":[61],"including":[62],"high":[63],"broad":[65],"dynamic":[66],"range,":[67],"minimal":[69],"reagent":[70],"addition":[71],"steps.":[72],"For":[73],"G(s)-coupled":[74],"antagonist":[75],"screens,":[76],"we":[77],"found":[78],"more":[83],"sensitive":[84],"HTS-compatible":[86],"techniques.":[87]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":8},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":8},{"year":2012,"cited_by_count":7}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}