{"id":"https://openalex.org/W2096534876","doi":"https://doi.org/10.1002/bit.260210809","title":"Theoretical and experimental analysis of a soluble enzyme membrane reactor","display_name":"Theoretical and experimental analysis of a soluble enzyme membrane reactor","publication_year":1979,"publication_date":"1979-08-01","ids":{"openalex":"https://openalex.org/W2096534876","doi":"https://doi.org/10.1002/bit.260210809","mag":"2096534876","pmid":"https://pubmed.ncbi.nlm.nih.gov/454808"},"language":"en","primary_location":{"id":"doi:10.1002/bit.260210809","doi":"https://doi.org/10.1002/bit.260210809","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.260210809","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102109451","display_name":"Guido Greco","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71267560","display_name":"University of Naples Federico II","ror":"https://ror.org/05290cv24","country_code":"IT","type":"education","lineage":["https://openalex.org/I71267560"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Guido Greco","raw_affiliation_strings":["Istituto di Principi di Ingegneria Chimica, Facolta\u0300 di Ingegneria, Universita\u0300 di Napoli, Italy","Istituto di Principi di Ingegneria Chimica, Facolt\u00e0 di Ingegneria, Universit\u00e0 di Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Istituto di Principi di Ingegneria Chimica, Facolta\u0300 di Ingegneria, Universita\u0300 di Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]},{"raw_affiliation_string":"Istituto di Principi di Ingegneria Chimica, Facolt\u00e0 di Ingegneria, Universit\u00e0 di Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111578396","display_name":"Francesco Alfani","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71267560","display_name":"University of Naples Federico II","ror":"https://ror.org/05290cv24","country_code":"IT","type":"education","lineage":["https://openalex.org/I71267560"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Francesco Alfani","raw_affiliation_strings":["Istituto di Principi di Ingegneria Chimica, Facolta\u0300 di Ingegneria, Universita\u0300 di Napoli, Italy","Istituto di Principi di Ingegneria Chimica, Facolt\u00e0 di Ingegneria, Universit\u00e0 di Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Istituto di Principi di Ingegneria Chimica, Facolta\u0300 di Ingegneria, Universita\u0300 di Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]},{"raw_affiliation_string":"Istituto di Principi di Ingegneria Chimica, Facolt\u00e0 di Ingegneria, Universit\u00e0 di Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109980834","display_name":"G. Iorio","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71267560","display_name":"University of Naples Federico II","ror":"https://ror.org/05290cv24","country_code":"IT","type":"education","lineage":["https://openalex.org/I71267560"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Gabriele Iorio","raw_affiliation_strings":["Istituto di Principi di Ingegneria Chimica, Facolta\u0300 di Ingegneria, Universita\u0300 di Napoli, Italy","Istituto di Principi di Ingegneria Chimica, Facolt\u00e0 di Ingegneria, Universit\u00e0 di Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Istituto di Principi di Ingegneria Chimica, Facolta\u0300 di Ingegneria, Universita\u0300 di Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]},{"raw_affiliation_string":"Istituto di Principi di Ingegneria Chimica, Facolt\u00e0 di Ingegneria, Universit\u00e0 di Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032518142","display_name":"Maria Cantarella","orcid":"https://orcid.org/0000-0002-0519-6757","observed_orcids":["https://orcid.org/0000-0002-0519-6757"]},"institutions":[{"id":"https://openalex.org/I71267560","display_name":"University of Naples Federico II","ror":"https://ror.org/05290cv24","country_code":"IT","type":"education","lineage":["https://openalex.org/I71267560"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Maria Cantarella","raw_affiliation_strings":["Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolta\u0300 di Scienze, Universita\u0300 di Napoli, Napoli, Italy","Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolt\u00e0 di Scienze, Universit\u00e0 di Napoli, Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolta\u0300 di Scienze, Universita\u0300 di Napoli, Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]},{"raw_affiliation_string":"Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolt\u00e0 di Scienze, Universit\u00e0 di Napoli, Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009787855","display_name":"Angela Formisano","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71267560","display_name":"University of Naples Federico II","ror":"https://ror.org/05290cv24","country_code":"IT","type":"education","lineage":["https://openalex.org/I71267560"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Angela Formisano","raw_affiliation_strings":["Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolta\u0300 di Scienze, Universita\u0300 di Napoli, Napoli, Italy","Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolt\u00e0 di Scienze, Universit\u00e0 di Napoli, Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolta\u0300 di Scienze, Universita\u0300 di Napoli, Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]},{"raw_affiliation_string":"Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolt\u00e0 di Scienze, Universit\u00e0 di Napoli, Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091436998","display_name":"Liliana Gianfreda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71267560","display_name":"University of Naples Federico II","ror":"https://ror.org/05290cv24","country_code":"IT","type":"education","lineage":["https://openalex.org/I71267560"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Liliana Gianfreda","raw_affiliation_strings":["Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolta\u0300 di Scienze, Universita\u0300 di Napoli, Napoli, Italy","Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolt\u00e0 di Scienze, Universit\u00e0 di Napoli, Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolta\u0300 di Scienze, Universita\u0300 di Napoli, Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]},{"raw_affiliation_string":"Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolt\u00e0 di Scienze, Universit\u00e0 di Napoli, Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054998624","display_name":"Rosaria Palescandolo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71267560","display_name":"University of Naples Federico II","ror":"https://ror.org/05290cv24","country_code":"IT","type":"education","lineage":["https://openalex.org/I71267560"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Rosaria Palescandolo","raw_affiliation_strings":["Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolta\u0300 di Scienze, Universita\u0300 di Napoli, Napoli, Italy","Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolt\u00e0 di Scienze, Universit\u00e0 di Napoli, Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolta\u0300 di Scienze, Universita\u0300 di Napoli, Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]},{"raw_affiliation_string":"Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolt\u00e0 di Scienze, Universit\u00e0 di Napoli, Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5000079690","display_name":"Vincenzo Scardi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71267560","display_name":"University of Naples Federico II","ror":"https://ror.org/05290cv24","country_code":"IT","type":"education","lineage":["https://openalex.org/I71267560"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Vincenzo Scardi","raw_affiliation_strings":["Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolta\u0300 di Scienze, Universita\u0300 di Napoli, Napoli, Italy","Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolt\u00e0 di Scienze, Universit\u00e0 di Napoli, Napoli, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolta\u0300 di Scienze, Universita\u0300 di Napoli, Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]},{"raw_affiliation_string":"Cattedra di Chimica delle Fermentazioni e Batteriologia Industriale, Facolt\u00e0 di Scienze, Universit\u00e0 di Napoli, Napoli, Italy","institution_ids":["https://openalex.org/I71267560"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5102109451"],"corresponding_institution_ids":["https://openalex.org/I71267560"],"apc_list":{"value":5060,"currency":"USD","value_usd":5060},"apc_paid":null,"fwci":1.0656,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.74652374,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"21","issue":"8","first_page":"1421","last_page":"1438"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9394999742507935,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9394999742507935,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10197","display_name":"Membrane Separation Technologies","score":0.04399999976158142,"subfield":{"id":"https://openalex.org/subfields/2312","display_name":"Water Science and Technology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.010700000450015068,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/enzyme-immobilization","display_name":"enzyme immobilization","score":0.8930000066757202},{"id":"https://openalex.org/keywords/ultrafiltration-membranes","display_name":"ultrafiltration membranes","score":0.6759999990463257},{"id":"https://openalex.org/keywords/soluble-enzyme","display_name":"soluble enzyme","score":0.49399998784065247},{"id":"https://openalex.org/keywords/membrane-reactor","display_name":"membrane reactor","score":0.8399999737739563},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5070000290870667},{"id":"https://openalex.org/keywords/unsteady-state","display_name":"unsteady state","score":0.4259999990463257},{"id":"https://openalex.org/keywords/molecular-weight-cutoff","display_name":"molecular weight cut-off","score":0.5230000019073486}],"concepts":[{"id":"https://openalex.org/C97488330","wikidata":"https://www.wikidata.org/wiki/Q906016","display_name":"Membrane reactor","level":3,"score":0.5967620611190796},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5536280870437622},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5324870944023132},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.45466485619544983},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.38749226927757263},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3236451745033264}],"mesh":[{"descriptor_ui":"D004798","descriptor_name":"Enzymes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004800","descriptor_name":"Enzymes, Immobilized","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005782","descriptor_name":"Gels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008433","descriptor_name":"Mathematics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008567","descriptor_name":"Membranes, Artificial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008956","descriptor_name":"Models, Chemical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012995","descriptor_name":"Solubility","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046911","descriptor_name":"Macromolecular Substances","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/bit.260210809","doi":"https://doi.org/10.1002/bit.260210809","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.260210809","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"pmid:454808","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/454808","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and bioengineering","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":7,"referenced_works":["https://openalex.org/W70700967","https://openalex.org/W195628285","https://openalex.org/W1497245978","https://openalex.org/W1973296070","https://openalex.org/W2090287045","https://openalex.org/W2161652691","https://openalex.org/W2235342184"],"related_works":["https://openalex.org/W2074039095","https://openalex.org/W195628285","https://openalex.org/W2006126324","https://openalex.org/W1976924165","https://openalex.org/W1999182115","https://openalex.org/W2075584969","https://openalex.org/W2103061877","https://openalex.org/W376076071","https://openalex.org/W2093849885","https://openalex.org/W2395292393"],"abstract_inverted_index":{"Recently":[0],"enzyme":[1,40,50,54,74,92,99,104,126,172,196],"immobilization":[2,122],"techniques":[3],"have":[4],"been":[5,57],"proposed":[6],"that":[7,83,157,199],"are":[8,162,200],"mainly":[9],"founded":[10],"on":[11,96],"the":[12,19,32,39,53,60,67,79,97,102,108,115,125,130,144,154,170,203],"formation":[13],"of":[14,22,169],"an":[15,23,27],"enzyme-gel":[16],"layer":[17],"onto":[18],"active":[20],"surface":[21,82],"ultrafiltration":[24,29],"membrane":[25,33,81,145,173],"within":[26,138],"unstirred":[28],"cell.":[30],"If":[31],"molecular-weight":[34],"cutoff":[35],"is":[36,135,176],"less":[37,113],"than":[38,114],"molecular":[41],"weight":[42],"and":[43,63,89,124,146],"hence":[44,90],"such":[45],"as":[46],"to":[47,66,87,91,160],"completely":[48],"prevent":[49],"permeation":[51],"(once":[52],"solution":[55],"has":[56],"charged":[58],"into":[59],"test":[61],"cell":[62],"pressure":[64],"applied":[65],"system),":[68],"a":[69,139,166,184],"time":[70],"progressive":[71],"increase":[72],"in":[73,107,129,190,202],"concentration":[75,105,197],"takes":[76,181],"place":[77],"at":[78,147,194],"upstream":[80,143],"can":[84],"eventually":[85],"lead":[86],"gelation":[88,116],"immobilization.":[93],"However,":[94],"depending":[95],"total":[98],"amount":[100],"fed,":[101],"maximum":[103],"achieved":[106],"unsteady":[109],"state":[110],"could":[111,186],"be":[112,187],"level.":[117],"In":[118,151],"this":[119,152],"situation,":[120],"no":[121,179],"occurs":[123],"still":[127,201],"remains":[128],"soluble":[131,171,204],"form":[132],"although":[133],"it":[134],"practically":[136],"confined":[137],"limited":[140],"region":[141],"immediately":[142],"fairly":[148],"high":[149,195],"concentrations.":[150],"paper,":[153],"experimental":[155],"conditions":[156],"allow":[158],"gelling":[159,180],"occur":[161],"discussed":[163],"together":[164],"with":[165],"theoretical":[167],"analysis":[168],"reactor":[174],"which":[175],"obtained":[177],"when":[178],"place.":[182],"Such":[183],"system":[185],"usefully":[188],"employed":[189],"performing":[191],"kinetic":[192],"analyses":[193],"levels":[198],"form.":[205]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}