{"id":"https://openalex.org/W2095383373","doi":"https://doi.org/10.1021/bi00505a023","title":"Ribonucleic acid-protein cross-linking in Escherichia coli ribosomes: (4-azidophenyl)glyoxal, a novel heterobifunctional reagent","display_name":"Ribonucleic acid-protein cross-linking in Escherichia coli ribosomes: (4-azidophenyl)glyoxal, a novel heterobifunctional reagent","publication_year":1981,"publication_date":"1981-01-01","ids":{"openalex":"https://openalex.org/W2095383373","doi":"https://doi.org/10.1021/bi00505a023","mag":"2095383373","pmid":"https://pubmed.ncbi.nlm.nih.gov/7008843"},"language":"en","primary_location":{"id":"doi:10.1021/bi00505a023","doi":"https://doi.org/10.1021/bi00505a023","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00505a023","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5034146328","display_name":"Samuel M. Politz","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Samuel M. Politz","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089776171","display_name":"Harry F. Noller","orcid":"https://orcid.org/0000-0002-2299-8700","observed_orcids":["https://orcid.org/0000-0002-2299-8700"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Harry F. Noller","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5058890279","display_name":"Paul D. McWhirter","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Paul D. McWhirter","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.5878,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.82757593,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"20","issue":"2","first_page":"372","last_page":"378"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heterobifunctional-reagents","display_name":"heterobifunctional reagents","score":0.8809999823570251},{"id":"https://openalex.org/keywords/30s-ribosomal-subunit","display_name":"30S ribosomal subunit","score":0.8880000114440918},{"id":"https://openalex.org/keywords/16s-rrna","display_name":"16S rRNA","score":0.8199999928474426},{"id":"https://openalex.org/keywords/escherichia-coli-ribosome","display_name":"Escherichia coli ribosome","score":0.8050000071525574},{"id":"https://openalex.org/keywords/ribosomal-proteins","display_name":"ribosomal proteins","score":0.6840000152587891}],"concepts":[{"id":"https://openalex.org/C2778407487","wikidata":"https://www.wikidata.org/wiki/Q14565201","display_name":"Altmetrics","level":2,"score":0.6720471382141113},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.6192234754562378},{"id":"https://openalex.org/C2777603720","wikidata":"https://www.wikidata.org/wiki/Q413465","display_name":"Glyoxal","level":2,"score":0.6019365787506104},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5788643956184387},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5091015696525574},{"id":"https://openalex.org/C2778805511","wikidata":"https://www.wikidata.org/wiki/Q1713","display_name":"Citation","level":2,"score":0.4763919711112976},{"id":"https://openalex.org/C518677369","wikidata":"https://www.wikidata.org/wiki/Q202833","display_name":"Social media","level":2,"score":0.4126392602920532},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.37679824233055115},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.3631412386894226},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3076017200946808},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.22177374362945557},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.09850993752479553},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000447","descriptor_name":"Aldehydes","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D001386","descriptor_name":"Azides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D003432","descriptor_name":"Cross-Linking Reagents","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006037","descriptor_name":"Glyoxal","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D006037","descriptor_name":"Glyoxal","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D006151","descriptor_name":"Guanosine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010658","descriptor_name":"Phenylglyoxal","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D010658","descriptor_name":"Phenylglyoxal","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D012269","descriptor_name":"Ribosomal Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012335","descriptor_name":"RNA, Ribosomal","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013056","descriptor_name":"Spectrophotometry, Ultraviolet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00505a023","doi":"https://doi.org/10.1021/bi00505a023","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00505a023","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:7008843","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7008843","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":40,"referenced_works":["https://openalex.org/W105515906","https://openalex.org/W1040351076","https://openalex.org/W1536076450","https://openalex.org/W1601065766","https://openalex.org/W1976895926","https://openalex.org/W1988018659","https://openalex.org/W1989742118","https://openalex.org/W1989844889","https://openalex.org/W1993461320","https://openalex.org/W1995455757","https://openalex.org/W1995506366","https://openalex.org/W2004791193","https://openalex.org/W2009708970","https://openalex.org/W2010075272","https://openalex.org/W2012515169","https://openalex.org/W2012795877","https://openalex.org/W2027019780","https://openalex.org/W2030653744","https://openalex.org/W2032302915","https://openalex.org/W2033002274","https://openalex.org/W2034277598","https://openalex.org/W2035168971","https://openalex.org/W2035741483","https://openalex.org/W2045877734","https://openalex.org/W2047720257","https://openalex.org/W2049623185","https://openalex.org/W2050055670","https://openalex.org/W2052656534","https://openalex.org/W2060026013","https://openalex.org/W2060203628","https://openalex.org/W2064874993","https://openalex.org/W2072947993","https://openalex.org/W2089687129","https://openalex.org/W2092948042","https://openalex.org/W2099187812","https://openalex.org/W2154765845","https://openalex.org/W2320083900","https://openalex.org/W2645584078","https://openalex.org/W2887735828","https://openalex.org/W2911944656"],"related_works":["https://openalex.org/W2060424033","https://openalex.org/W2031094513","https://openalex.org/W2012100932","https://openalex.org/W1987528168","https://openalex.org/W2030123313","https://openalex.org/W1987051446","https://openalex.org/W1995506366","https://openalex.org/W2060203628","https://openalex.org/W1596998060","https://openalex.org/W1972318245"],"abstract_inverted_index":{"We":[0],"have":[1],"used":[2],"the":[3,23,39,98,109],"heterobifunctional":[4],"reagent":[5,24],"(4-azidophenyl)glyoxal":[6],"(APG)":[7],"to":[8,11,102,114],"cross-link":[9],"RNA":[10,116],"protein":[12],"in":[13,45,69,82,92],"Escherichia":[14],"coli":[15],"30S":[16,83,100,110],"ribosomal":[17],"subunits.":[18],"Synthesis":[19],"and":[20,71],"characterization":[21],"of":[22,78,88,94,97,108],"are":[25,85],"described.":[26],"Like":[27],"other":[28],"dicarbonyl":[29],"reagents":[30],"(e.g.,":[31],"kethoxal),":[32],"APG":[33,46,76,90],"reacts":[34],"specifically":[35],"with":[36,50,66],"guanosine":[37],"among":[38],"four":[40],"ribonucleosides.":[41],"The":[42],"azido":[43],"group":[44],"can":[47],"be":[48],"photolyzed":[49],"UV":[51],"light":[52],"(lambda":[53],"greater":[54],"than":[55],"300":[56],"nm),":[57],"yielding":[58],"an":[59],"unstable":[60],"nitrene":[61],"which":[62],"is":[63,112],"potentially":[64],"reactive":[65],"many":[67],"groups":[68],"proteins":[70,101,111],"nucleic":[72],"acids.":[73],"Conditions":[74],"for":[75],"modification":[77],"guanylic":[79],"acid":[80],"residues":[81],"subunits":[84],"described;":[86],"photolysis":[87],"bound":[89],"results":[91],"cross-linking":[93],"approximately":[95],"5%":[96],"total":[99],"16S":[103,115],"RNA.":[104],"A":[105],"specific":[106],"subset":[107],"cross-linked":[113],"by":[117],"APG.":[118]},"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}