{"id":"https://openalex.org/W2094875705","doi":"https://doi.org/10.1016/s0736-0266(01)00147-4","title":"Macrophage exposure to particulate titanium induces phosphorylation of the protein tyrosine kinase lyn and the phospholipases C\u03b3\u20101 and C\u03b3\u20102","display_name":"Macrophage exposure to particulate titanium induces phosphorylation of the protein tyrosine kinase lyn and the phospholipases C\u03b3\u20101 and C\u03b3\u20102","publication_year":2002,"publication_date":"2002-05-01","ids":{"openalex":"https://openalex.org/W2094875705","doi":"https://doi.org/10.1016/s0736-0266(01)00147-4","mag":"2094875705","pmid":"https://pubmed.ncbi.nlm.nih.gov/12038621"},"language":"en","primary_location":{"id":"doi:10.1016/s0736-0266(01)00147-4","doi":"https://doi.org/10.1016/s0736-0266(01)00147-4","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0736-0266(01)00147-4","pdf_url":null,"source":{"id":"https://openalex.org/S12558119","display_name":"Journal of Orthopaedic Research\u00ae","issn_l":"0736-0266","issn":["0736-0266","1554-527X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Orthopaedic Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5084210416","display_name":"Phillip Lee Palmbos","orcid":"https://orcid.org/0000-0002-3134-2965","observed_orcids":["https://orcid.org/0000-0002-3134-2965"]},"institutions":[{"id":"https://openalex.org/I163795733","display_name":"Calvin University","ror":"https://ror.org/05r0q9p84","country_code":"US","type":"education","lineage":["https://openalex.org/I163795733"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Phillip L. Palmbos","raw_affiliation_strings":["Department of Biology, Calvin College, Grand Rapids, MI 49546, USA","Department of Biology, Calvin College, 3201 Burton Street SE, Grand Rapids, MI 49546, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Calvin College, Grand Rapids, MI 49546, USA","institution_ids":["https://openalex.org/I163795733"]},{"raw_affiliation_string":"Department of Biology, Calvin College, 3201 Burton Street SE, Grand Rapids, MI 49546, USA","institution_ids":["https://openalex.org/I163795733"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014483260","display_name":"Mark J. Sytsma","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I163795733","display_name":"Calvin University","ror":"https://ror.org/05r0q9p84","country_code":"US","type":"education","lineage":["https://openalex.org/I163795733"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark J. Sytsma","raw_affiliation_strings":["Department of Biology, Calvin College, 3201 Burton Street SE, Grand Rapids, MI 49546, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Calvin College, 3201 Burton Street SE, Grand Rapids, MI 49546, USA","institution_ids":["https://openalex.org/I163795733"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028197996","display_name":"David H. DeHeer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I163795733","display_name":"Calvin University","ror":"https://ror.org/05r0q9p84","country_code":"US","type":"education","lineage":["https://openalex.org/I163795733"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"David H. Deheer","raw_affiliation_strings":["Department of Biology, Calvin College, 3201 Burton Street SE, Grand Rapids, MI 49546, USA","Grand Rapids Orthopaedic Surgery Residency Program, 1840 Wealthy Street SE, Grand Rapids, MI 49506, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Calvin College, 3201 Burton Street SE, Grand Rapids, MI 49546, USA","institution_ids":["https://openalex.org/I163795733"]},{"raw_affiliation_string":"Grand Rapids Orthopaedic Surgery Residency Program, 1840 Wealthy Street SE, Grand Rapids, MI 49506, USA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073858351","display_name":"Joy D. Bonnema","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I163795733","display_name":"Calvin University","ror":"https://ror.org/05r0q9p84","country_code":"US","type":"education","lineage":["https://openalex.org/I163795733"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joy D. Bonnema","raw_affiliation_strings":["Department of Biology, Calvin College, 3201 Burton Street SE, Grand Rapids, MI 49546, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Calvin College, 3201 Burton Street SE, Grand Rapids, MI 49546, USA","institution_ids":["https://openalex.org/I163795733"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5028197996"],"corresponding_institution_ids":["https://openalex.org/I163795733"],"apc_list":{"value":3970,"currency":"USD","value_usd":3970},"apc_paid":null,"fwci":1.2696,"has_fulltext":false,"cited_by_count":16,"citation_normalized_percentile":{"value":0.81962256,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"20","issue":"3","first_page":"483","last_page":"489"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10512","display_name":"Orthopaedic implants and arthroplasty","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10512","display_name":"Orthopaedic implants and arthroplasty","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11293","display_name":"Orthopedic Infections and Treatments","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10105","display_name":"Osteoarthritis Treatment and Mechanisms","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/titanium-particles","display_name":"titanium particles","score":0.8109999895095825},{"id":"https://openalex.org/keywords/macrophage-activation","display_name":"macrophage activation","score":0.9190000295639038},{"id":"https://openalex.org/keywords/aseptic-loosening","display_name":"aseptic loosening","score":0.8659999966621399},{"id":"https://openalex.org/keywords/total-joint-arthroplasty","display_name":"total joint arthroplasty","score":0.878000020980835},{"id":"https://openalex.org/keywords/wear-particles","display_name":"wear debris","score":0.9179999828338623},{"id":"https://openalex.org/keywords/tyrosine-kinase-signaling","display_name":"tyrosine kinase signaling","score":0.8149999976158142},{"id":"https://openalex.org/keywords/lyn-kinase","display_name":"Lyn kinase","score":0.7319999933242798},{"id":"https://openalex.org/keywords/tnf-alpha-secretion","display_name":"TNF-alpha secretion","score":0.6819999814033508},{"id":"https://openalex.org/keywords/prostaglandin-e2","display_name":"prostaglandin E2","score":0.9580000042915344},{"id":"https://openalex.org/keywords/herbimycin-a","display_name":"herbimycin A","score":0.8299999833106995},{"id":"https://openalex.org/keywords/genistein","display_name":"genistein","score":0.9980000257492065},{"id":"https://openalex.org/keywords/particle-coating","display_name":"particle coating","score":0.8679999709129333},{"id":"https://openalex.org/keywords/signal-transduction","display_name":"signal transduction","score":0.7070000171661377}],"concepts":[{"id":"https://openalex.org/C175818844","wikidata":"https://www.wikidata.org/wiki/Q2463389","display_name":"LYN","level":4,"score":0.7307329177856445},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.5914550423622131},{"id":"https://openalex.org/C2781282405","wikidata":"https://www.wikidata.org/wiki/Q2035084","display_name":"Osteolysis","level":2,"score":0.5640248656272888},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.556488573551178},{"id":"https://openalex.org/C42362537","wikidata":"https://www.wikidata.org/wiki/Q87099740","display_name":"Tyrosine kinase","level":3,"score":0.535556972026825},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5001201629638672},{"id":"https://openalex.org/C2777553839","wikidata":"https://www.wikidata.org/wiki/Q14330986","display_name":"Tyrosine phosphorylation","level":3,"score":0.49976301193237305},{"id":"https://openalex.org/C2779244956","wikidata":"https://www.wikidata.org/wiki/Q184204","display_name":"Macrophage","level":3,"score":0.46905234456062317},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.41386669874191284},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.40402764081954956},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3920733630657196},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3420753479003906},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2783142328262329},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2711849808692932},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.23405751585960388},{"id":"https://openalex.org/C141071460","wikidata":"https://www.wikidata.org/wiki/Q40821","display_name":"Surgery","level":1,"score":0.0},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004789","descriptor_name":"Enzyme Activation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008264","descriptor_name":"Macrophages","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D008264","descriptor_name":"Macrophages","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010587","descriptor_name":"Phagocytosis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D010738","descriptor_name":"Type C Phospholipases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D014025","descriptor_name":"Titanium","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D018836","descriptor_name":"Inflammation Mediators","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019061","descriptor_name":"src-Family Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D051966","descriptor_name":"Phospholipase C gamma","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0736-0266(01)00147-4","doi":"https://doi.org/10.1016/s0736-0266(01)00147-4","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0736-0266(01)00147-4","pdf_url":null,"source":{"id":"https://openalex.org/S12558119","display_name":"Journal of Orthopaedic Research\u00ae","issn_l":"0736-0266","issn":["0736-0266","1554-527X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Orthopaedic Research","raw_type":"journal-article"},{"id":"pmid:12038621","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12038621","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of orthopaedic research : official publication of the Orthopaedic Research Society","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8371999859809875}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":40,"referenced_works":["https://openalex.org/W16386120","https://openalex.org/W1493564682","https://openalex.org/W1530340727","https://openalex.org/W1545383294","https://openalex.org/W1653828571","https://openalex.org/W1656644300","https://openalex.org/W1897432177","https://openalex.org/W1965344276","https://openalex.org/W1966008657","https://openalex.org/W1971820762","https://openalex.org/W1978854676","https://openalex.org/W1980301203","https://openalex.org/W1989542596","https://openalex.org/W1990783034","https://openalex.org/W1992605956","https://openalex.org/W1995900983","https://openalex.org/W1999357005","https://openalex.org/W1999880807","https://openalex.org/W2005216725","https://openalex.org/W2008511214","https://openalex.org/W2045813307","https://openalex.org/W2046768924","https://openalex.org/W2047329367","https://openalex.org/W2049246519","https://openalex.org/W2051774115","https://openalex.org/W2053917902","https://openalex.org/W2060880134","https://openalex.org/W2074739298","https://openalex.org/W2078618689","https://openalex.org/W2092135614","https://openalex.org/W2112397260","https://openalex.org/W2119569059","https://openalex.org/W2119958553","https://openalex.org/W2132646264","https://openalex.org/W2135600569","https://openalex.org/W2192686175","https://openalex.org/W2225962314","https://openalex.org/W2261592228","https://openalex.org/W2404286363","https://openalex.org/W4238565563"],"related_works":["https://openalex.org/W2119569059","https://openalex.org/W2078511674","https://openalex.org/W1513192165","https://openalex.org/W2115757302","https://openalex.org/W2034306778","https://openalex.org/W4247369302","https://openalex.org/W2071284555","https://openalex.org/W1920320579","https://openalex.org/W2158491518","https://openalex.org/W4224060917"],"abstract_inverted_index":{"A":[0,61],"frequent":[1],"long-term":[2],"complication":[3],"of":[4,14,36,66,78,105],"total":[5],"joint":[6],"arthroplasty":[7],"is":[8],"aseptic":[9],"loosening,":[10],"the":[11,27,34,109],"end":[12],"result":[13],"wear":[15],"debris":[16],"production,":[17,42],"synovial":[18],"macrophage":[19,38,100],"activation,":[20],"inflammatory":[21],"mediator":[22,41],"release,":[23],"and":[24,40,52,83,113,116,123],"osteolysis":[25],"about":[26],"implant-bone":[28],"or":[29,62,147],"cement-bone":[30],"interface.":[31],"To":[32],"elucidate":[33],"mechanisms":[35],"particle-induced":[37],"activation":[39],"we":[43],"studied":[44],"early":[45],"signal":[46,151],"transduction":[47,152],"events":[48],"using":[49],"J774A.1":[50],"macrophages":[51,58],"3":[53],"microm":[54],"titanium":[55,72,143],"particles.":[56],"Treating":[57],"with":[59,142,145],"herbimycin":[60],"genistein,":[63],"two":[64],"inhibitors":[65],"protein":[67],"tyrosine":[68],"kinases":[69],"(PTKs),":[70],"inhibited":[71],"phagocytosis":[73],"as":[74,76],"well":[75],"secretion":[77],"tumor":[79],"necrosis":[80],"factor-alpha":[81],"(TNF-alpha)":[82],"prostaglandin-E2":[84],"(PGE2)":[85],"in":[86],"a":[87,95],"dose-dependent":[88],"manner.":[89],"Both":[90],"processes":[91],"therefore":[92],"depend":[93],"on":[94,155],"PTK":[96],"signaling":[97],"cascade.":[98],"Specifically,":[99],"exposure":[101],"to":[102,125],"titanium-induced":[103],"phosphorylation":[104,134],"multiple":[106],"proteins":[107],"including":[108],"Src":[110],"kinase":[111],"Lyn":[112],"phospholipase":[114],"Cgamma-1":[115],"Cgamma-2.":[117],"Phosphorylation":[118],"peaked":[119],"within":[120,127],"2":[121],"min":[122],"returned":[124],"baseline":[126],"45":[128],"min.":[129],"Similar":[130],"but":[131],"not":[132],"identical":[133],"patterns":[135],"were":[136,140],"obtained":[137],"when":[138],"cells":[139],"stimulated":[141],"preincubated":[144],"serum":[146],"albumin,":[148],"suggesting":[149],"distinct":[150],"pathways":[153],"dependent":[154],"particle":[156],"coating.":[157]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}