{"id":"https://openalex.org/W2094772935","doi":"https://doi.org/10.1002/bip.360291406","title":"Linear dichroism characteristics of ethidium\u2013and proflavine\u2013supercoiled DNA complexes","display_name":"Linear dichroism characteristics of ethidium\u2013and proflavine\u2013supercoiled DNA complexes","publication_year":1990,"publication_date":"1990-12-01","ids":{"openalex":"https://openalex.org/W2094772935","doi":"https://doi.org/10.1002/bip.360291406","mag":"2094772935","pmid":"https://pubmed.ncbi.nlm.nih.gov/2145043"},"language":"en","primary_location":{"id":"doi:10.1002/bip.360291406","doi":"https://doi.org/10.1002/bip.360291406","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bip.360291406","pdf_url":null,"source":{"id":"https://openalex.org/S56808608","display_name":"Biopolymers","issn_l":"0006-3525","issn":["0006-3525","1097-0282"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112065925","display_name":"Charles E. Swenberg","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Charles E. Swenberg","raw_affiliation_strings":["Radiation Biochemistry Department, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20814"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Radiation Biochemistry Department, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20814","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072313679","display_name":"Susan E. Carberry","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I57206974","display_name":"New York University","ror":"https://ror.org/0190ak572","country_code":"US","type":"education","lineage":["https://openalex.org/I57206974"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Susan E. Carberry","raw_affiliation_strings":["Chemistry Department and Radiation and Solid State Laboratory, New York University, New York, New York 10003"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Department and Radiation and Solid State Laboratory, New York University, New York, New York 10003","institution_ids":["https://openalex.org/I57206974"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018460703","display_name":"Nicholas E. Geacintov","orcid":"https://orcid.org/0000-0002-7961-2231","observed_orcids":["https://orcid.org/0000-0002-7961-2231"]},"institutions":[{"id":"https://openalex.org/I57206974","display_name":"New York University","ror":"https://ror.org/0190ak572","country_code":"US","type":"education","lineage":["https://openalex.org/I57206974"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nicholas E. Geacintov","raw_affiliation_strings":["Chemistry Department and Radiation and Solid State Laboratory, New York University, New York, New York 10003"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Department and Radiation and Solid State Laboratory, New York University, New York, New York 10003","institution_ids":["https://openalex.org/I57206974"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4150,"currency":"USD","value_usd":4150},"apc_paid":null,"fwci":0.7127,"has_fulltext":false,"cited_by_count":15,"citation_normalized_percentile":{"value":0.68860204,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"29","issue":"14","first_page":"1735","last_page":"1744"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10163","display_name":"Metal complexes synthesis and properties","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13105","display_name":"Synthesis and pharmacology of benzodiazepine derivatives","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/linear-dichroism","display_name":"linear dichroism","score":0.968999981880188},{"id":"https://openalex.org/keywords/supercoiled-dna","display_name":"supercoiled DNA","score":0.8830000162124634},{"id":"https://openalex.org/keywords/ethidium-bromide","display_name":"ethidium bromide","score":0.9760000109672546},{"id":"https://openalex.org/keywords/proflavine","display_name":"proflavine","score":1.0},{"id":"https://openalex.org/keywords/dna-intercalation","display_name":"DNA intercalation","score":0.8740000128746033},{"id":"https://openalex.org/keywords/dna-unwinding","display_name":"DNA unwinding","score":0.8560000061988831},{"id":"https://openalex.org/keywords/drug-dna-binding","display_name":"drug-DNA binding","score":0.4790000021457672},{"id":"https://openalex.org/keywords/flow-linear-dichroism","display_name":"flow linear dichroism","score":0.7490000128746033},{"id":"https://openalex.org/keywords/hydrodynamic-shape","display_name":"hydrodynamic shape","score":0.5490000247955322},{"id":"https://openalex.org/keywords/calf-thymus-dna","display_name":"calf thymus DNA","score":0.7360000014305115},{"id":"https://openalex.org/keywords/sedimentation-coefficient","display_name":"sedimentation coefficient","score":0.5759999752044678}],"concepts":[{"id":"https://openalex.org/C2776594999","wikidata":"https://www.wikidata.org/wiki/Q420454","display_name":"Proflavine","level":3,"score":0.9024049043655396},{"id":"https://openalex.org/C2776907736","wikidata":"https://www.wikidata.org/wiki/Q408634","display_name":"Ethidium bromide","level":3,"score":0.8463472127914429},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8444331884384155},{"id":"https://openalex.org/C146593782","wikidata":"https://www.wikidata.org/wiki/Q6553431","display_name":"Linear dichroism","level":3,"score":0.7855191230773926},{"id":"https://openalex.org/C133571119","wikidata":"https://www.wikidata.org/wiki/Q899102","display_name":"Circular dichroism","level":2,"score":0.701783299446106},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6771063208580017},{"id":"https://openalex.org/C98015330","wikidata":"https://www.wikidata.org/wiki/Q907315","display_name":"Absorbance","level":2,"score":0.6356877088546753},{"id":"https://openalex.org/C2779408652","wikidata":"https://www.wikidata.org/wiki/Q7643415","display_name":"Superhelix","level":5,"score":0.5675963163375854},{"id":"https://openalex.org/C185581394","wikidata":"https://www.wikidata.org/wiki/Q145911","display_name":"Base pair","level":3,"score":0.5454389452934265},{"id":"https://openalex.org/C105600476","wikidata":"https://www.wikidata.org/wiki/Q1195643","display_name":"Dichroism","level":2,"score":0.4644043445587158},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.4542856216430664},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.4379676282405853},{"id":"https://openalex.org/C158456008","wikidata":"https://www.wikidata.org/wiki/Q412639","display_name":"DNA supercoil","level":4,"score":0.4101313650608063},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.39199334383010864},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.15535980463027954},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1110139787197113},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.09159514307975769},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C73573662","wikidata":"https://www.wikidata.org/wiki/Q130996","display_name":"DNA replication","level":3,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002627","descriptor_name":"Chemistry, Physical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004278","descriptor_name":"DNA, Superhelical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004996","descriptor_name":"Ethidium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010584","descriptor_name":"Bacteriophage phi X 174","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011370","descriptor_name":"Proflavine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013057","descriptor_name":"Spectrum Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055598","descriptor_name":"Chemical Phenomena","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/bip.360291406","doi":"https://doi.org/10.1002/bip.360291406","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bip.360291406","pdf_url":null,"source":{"id":"https://openalex.org/S56808608","display_name":"Biopolymers","issn_l":"0006-3525","issn":["0006-3525","1097-0282"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"journal-article"},{"id":"pmid:2145043","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2145043","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":44,"referenced_works":["https://openalex.org/W28696296","https://openalex.org/W166121322","https://openalex.org/W258061546","https://openalex.org/W570073207","https://openalex.org/W1487427081","https://openalex.org/W1513717105","https://openalex.org/W1532331378","https://openalex.org/W1963799772","https://openalex.org/W1969094530","https://openalex.org/W1969583871","https://openalex.org/W1971670546","https://openalex.org/W1972220947","https://openalex.org/W1981445345","https://openalex.org/W1987758751","https://openalex.org/W1988908879","https://openalex.org/W2001457684","https://openalex.org/W2001604634","https://openalex.org/W2003368094","https://openalex.org/W2004165111","https://openalex.org/W2008538853","https://openalex.org/W2012002884","https://openalex.org/W2012181402","https://openalex.org/W2028563796","https://openalex.org/W2034089464","https://openalex.org/W2043701757","https://openalex.org/W2044432299","https://openalex.org/W2050573762","https://openalex.org/W2050704540","https://openalex.org/W2054924813","https://openalex.org/W2056252681","https://openalex.org/W2066328915","https://openalex.org/W2077944846","https://openalex.org/W2089444942","https://openalex.org/W2089619141","https://openalex.org/W2090629729","https://openalex.org/W2134961349","https://openalex.org/W2145771605","https://openalex.org/W2156404207","https://openalex.org/W2170193782","https://openalex.org/W2171642857","https://openalex.org/W2317259830","https://openalex.org/W3022113655","https://openalex.org/W4239988172","https://openalex.org/W4293088719"],"related_works":["https://openalex.org/W2050704540","https://openalex.org/W2018101380","https://openalex.org/W2144312421","https://openalex.org/W1999959833","https://openalex.org/W1963799772","https://openalex.org/W2044134629","https://openalex.org/W3007273216","https://openalex.org/W2104696676","https://openalex.org/W1548941751","https://openalex.org/W1983682817"],"abstract_inverted_index":{"A":[0],"flow":[1,254,295],"linear":[2,42,48,53],"dichroism":[3,49],"technique":[4],"is":[5,120,150,156,220],"utilized":[6],"to":[7,41,72,107,152,177,223,249,274,279],"study":[8],"the":[9,16,33,46,60,76,93,98,124,127,159,168,178,188,192,230,253,260,269,282,287,294,300,307,313,327,331,343],"unwinding":[10],"of":[11,18,35,89,95,100,126,142,161,164,170,190,194,240,262,271,286,299,310,312,346],"supercoiled":[12,96,275,314],"DNA":[13,79,179,231,291,315,348],"induced":[14],"by":[15,136,204],"binding":[17],"ethidium":[19],"bromide":[20],"(EB)":[21],"and":[22,66,115,131,233,243,264,290,325],"proflavine":[23],"(PF)":[24],"at":[25,59,64,102,139,146,173,197,213,303],"different":[26],"ratios":[27],"r":[28,112,119,162,214,267],"(drug":[29],"added/DNA":[30],"base).":[31],"In":[32,92,185],"case":[34,94,189,270],"either":[36],"EB":[37,154,272,288,333,352],"or":[38,68,83,199],"PF":[39],"bound":[40,273,332,351],"calf":[43],"thymus":[44],"DNA,":[45,97,276],"reduced":[47],"signals":[50,329],"LD/A":[51,101,145,171,195],"(LD:":[52],"dichroism;":[54],"A:":[55],"absorbance,":[56],"both":[57,209,246],"measured":[58,135],"same":[61],"wavelength),":[62],"determined":[63,172,196],"258,":[65],"520":[67,147],"462":[69,200],"nm":[70,104,175,201,305],"(corresponding":[71],"contributions":[73],"predominantly":[74],"from":[75],"partially":[77],"oriented":[78,248],"bases,":[80],"intercalated":[81],"EB,":[82],"PF,":[84,191],"respectively)":[85],"are":[86,202,236,247,277],"nearly":[87],"independent":[88],"drug":[90,234],"concentration.":[91],"magnitude":[99,169,298],"258":[103,174,198,304],"first":[105],"increases":[106],"a":[108,182,224],"maximum":[109],"value":[110],"near":[111],"=":[113,215],"0.04-0.05,":[114],"then":[116],"decreases":[117],"as":[118,340,342],"increased":[121],"further,":[122],"mimicking":[123],"behavior":[125,219],"sedimentation":[128],"coefficients,":[129],"viscosities,":[130],"gel":[132],"electrophoresis":[133],"patterns":[134],"other":[137],"workers":[138],"similar":[140,205,250],"values":[141,163],"r.":[143],"However,":[144],"nm,":[148],"which":[149,167,229,245],"due":[151,176],"DNA-bound":[153],"molecules,":[155],"constant":[157],"within":[158],"range":[160],"0.02-0.06":[165],"in":[166,187,228,244,252,259,268,281,293,318],"bases":[180,232,292],"exhibits":[181],"pronounced":[183,211],"maximum.":[184],"contrast,":[186],"magnitudes":[193],"characterized":[203],"dependencies":[206,261],"on":[207,238,266,321],"r,":[208],"exhibiting":[210],"maxima":[212],"0.05;":[216],"this":[217,337],"parallel":[218],"expected":[221],"according":[222],"simple":[225],"intercalation":[226],"model":[227],"molecules":[235,289,316,334],"stacked":[237],"top":[239],"one":[241],"another,":[242],"extents":[251],"gradient.":[255,296],"The":[256,297],"unexpected":[257],"differences":[258,280],"(LD/A)258":[263],"(LD/A)520":[265],"attributed":[278],"net":[283],"overall":[284,308],"alignment":[285,345],"LD":[301,328],"signal":[302],"reflects":[306],"degree":[309],"orientation":[311,338],"that,":[317],"turn,":[319],"depends":[320],"their":[322],"hydrodynamic":[323],"shapes":[324],"sizes;":[326],"characterizing":[330],"may":[335],"reflect":[336],"also,":[339],"well":[341],"partial":[344],"individual":[347],"segments":[349],"containing":[350],"molecules.(ABSTRACT":[353],"TRUNCATED":[354],"AT":[355],"400":[356],"WORDS)":[357]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}