{"id":"https://openalex.org/W2094694252","doi":"https://doi.org/10.1074/jbc.270.24.14705","title":"Role of the Enzyme Calmodulin-binding Domain in Membrane Association and Phospholipid Inhibition of Endothelial Nitric Oxide Synthase","display_name":"Role of the Enzyme Calmodulin-binding Domain in Membrane Association and Phospholipid Inhibition of Endothelial Nitric Oxide Synthase","publication_year":1995,"publication_date":"1995-06-01","ids":{"openalex":"https://openalex.org/W2094694252","doi":"https://doi.org/10.1074/jbc.270.24.14705","mag":"2094694252","pmid":"https://pubmed.ncbi.nlm.nih.gov/7540177"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.270.24.14705","doi":"https://doi.org/10.1074/jbc.270.24.14705","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.270.24.14705","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.270.24.14705","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111946505","display_name":"Richard C. Venema","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Richard C. Venema","raw_affiliation_strings":["Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322, USA","institution_ids":["https://openalex.org/I150468666"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049080472","display_name":"Hassan S. Sayegh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hassan S. Sayegh","raw_affiliation_strings":["Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322","From the (1)Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322","institution_ids":["https://openalex.org/I150468666"]},{"raw_affiliation_string":"From the (1)Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322","institution_ids":["https://openalex.org/I150468666"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043815002","display_name":"Jean\u2010Fran\u00e7ois Arnal","orcid":"https://orcid.org/0000-0003-2229-6300","observed_orcids":["https://orcid.org/0000-0003-2229-6300"]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jean-Franois Arnal","raw_affiliation_strings":["Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322","From the (1)Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322","institution_ids":["https://openalex.org/I150468666"]},{"raw_affiliation_string":"From the (1)Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322","institution_ids":["https://openalex.org/I150468666"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5011627807","display_name":"David Glenn Harrison","orcid":"https://orcid.org/0000-0001-9363-6451","observed_orcids":["https://orcid.org/0000-0001-9363-6451"]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David G. Harrison","raw_affiliation_strings":["Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322","From the (1)Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322","institution_ids":["https://openalex.org/I150468666"]},{"raw_affiliation_string":"From the (1)Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia 30322","institution_ids":["https://openalex.org/I150468666"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5111946505"],"corresponding_institution_ids":["https://openalex.org/I150468666"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":5.8293,"has_fulltext":false,"cited_by_count":96,"citation_normalized_percentile":{"value":0.96963277,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"270","issue":"24","first_page":"14705","last_page":"14711"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10155","display_name":"Nitric Oxide and Endothelin Effects","score":0.7616999745368958,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10155","display_name":"Nitric Oxide and Endothelin Effects","score":0.7616999745368958,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.22849999368190765,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.0038999998942017555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/endothelial-nitric-oxide-synthase","display_name":"endothelial nitric oxide synthase","score":1.0},{"id":"https://openalex.org/keywords/calmodulin-binding-domain","display_name":"calmodulin-binding domain","score":0.9850000143051147},{"id":"https://openalex.org/keywords/calmodulin","display_name":"calmodulin","score":0.8790000081062317},{"id":"https://openalex.org/keywords/membrane-association","display_name":"membrane association","score":0.8769999742507935},{"id":"https://openalex.org/keywords/anionic-phospholipids","display_name":"anionic phospholipids","score":0.8970000147819519},{"id":"https://openalex.org/keywords/phosphatidylserine","display_name":"phosphatidylserine","score":0.9990000128746033},{"id":"https://openalex.org/keywords/enzyme-inhibition","display_name":"enzyme inhibition","score":0.6380000114440918},{"id":"https://openalex.org/keywords/electrostatic-interactions","display_name":"electrostatic interactions","score":0.8619999885559082},{"id":"https://openalex.org/keywords/protein-myristoylation","display_name":"myristoylation","score":0.8679999709129333},{"id":"https://openalex.org/keywords/cytoplasmic-enzymes","display_name":"cytosolic enzyme","score":0.49799999594688416},{"id":"https://openalex.org/keywords/lipid-bilayer-binding","display_name":"lipid bilayer binding","score":0.5529999732971191},{"id":"https://openalex.org/keywords/protein-lipid-interactions","display_name":"protein-lipid interactions","score":0.574999988079071}],"concepts":[{"id":"https://openalex.org/C29688787","wikidata":"https://www.wikidata.org/wiki/Q409145","display_name":"Calmodulin","level":3,"score":0.5798504948616028},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5769423842430115},{"id":"https://openalex.org/C2778326061","wikidata":"https://www.wikidata.org/wiki/Q1761022","display_name":"Enos","level":4,"score":0.5765887498855591},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5313003063201904},{"id":"https://openalex.org/C2778918659","wikidata":"https://www.wikidata.org/wiki/Q186915","display_name":"Phospholipid","level":3,"score":0.45838800072669983},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4527684450149536},{"id":"https://openalex.org/C2777622882","wikidata":"https://www.wikidata.org/wiki/Q417619","display_name":"Nitric oxide synthase","level":3,"score":0.4222145080566406},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.2513759136199951}],"mesh":[{"descriptor_ui":"D000594","descriptor_name":"Amino Acid Oxidoreductases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D000594","descriptor_name":"Amino Acid Oxidoreductases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000594","descriptor_name":"Amino Acid Oxidoreductases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002147","descriptor_name":"Calmodulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004730","descriptor_name":"Endothelium, Vascular","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010718","descriptor_name":"Phosphatidylserines","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010718","descriptor_name":"Phosphatidylserines","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016367","descriptor_name":"Baculoviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018411","descriptor_name":"Spodoptera","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019001","descriptor_name":"Nitric Oxide Synthase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.270.24.14705","doi":"https://doi.org/10.1074/jbc.270.24.14705","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.270.24.14705","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:7540177","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7540177","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.270.24.14705","doi":"https://doi.org/10.1074/jbc.270.24.14705","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.270.24.14705","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":44,"referenced_works":["https://openalex.org/W38526789","https://openalex.org/W191339276","https://openalex.org/W1484977141","https://openalex.org/W1495970501","https://openalex.org/W1506302012","https://openalex.org/W1507344018","https://openalex.org/W1509605247","https://openalex.org/W1531042454","https://openalex.org/W1565185374","https://openalex.org/W1565803037","https://openalex.org/W1595738876","https://openalex.org/W1599900714","https://openalex.org/W1605694747","https://openalex.org/W1667656237","https://openalex.org/W1913590823","https://openalex.org/W1968901680","https://openalex.org/W1973018387","https://openalex.org/W1983649348","https://openalex.org/W2004424023","https://openalex.org/W2008264122","https://openalex.org/W2010100459","https://openalex.org/W2020471821","https://openalex.org/W2021334430","https://openalex.org/W2032062053","https://openalex.org/W2037983802","https://openalex.org/W2042782418","https://openalex.org/W2050410538","https://openalex.org/W2055578834","https://openalex.org/W2059151280","https://openalex.org/W2059379142","https://openalex.org/W2060913542","https://openalex.org/W2064951152","https://openalex.org/W2076657129","https://openalex.org/W2092478589","https://openalex.org/W2092884273","https://openalex.org/W2116628043","https://openalex.org/W2123670331","https://openalex.org/W2134785329","https://openalex.org/W2151777308","https://openalex.org/W2162692258","https://openalex.org/W2177270915","https://openalex.org/W2206789948","https://openalex.org/W2219883988","https://openalex.org/W2408580855"],"related_works":["https://openalex.org/W2074326059","https://openalex.org/W1964839275","https://openalex.org/W1995391066","https://openalex.org/W2081884636","https://openalex.org/W3176846529","https://openalex.org/W2263559773","https://openalex.org/W3169115136","https://openalex.org/W1977889124","https://openalex.org/W2167042104","https://openalex.org/W2055114314"],"abstract_inverted_index":{"Endothelial":[0],"nitric":[1],"oxide":[2],"synthase":[3],"(eNOS)":[4],"is":[5,85,101,193],"a":[6,38,184,187],"calmodulin":[7],"(CaM)-dependent,":[8],"membrane-associated,":[9],"myristoylated":[10],"enzyme,":[11],"which":[12],"has":[13],"an":[14],"important":[15,217],"role":[16],"in":[17,37,146,158,195,211],"regulation":[18],"of":[19,32,82,116,124,130,140,151,160,163,177,237],"vascular":[20],"tone":[21],"and":[22,29,43,208],"platelet":[23],"aggregation.":[24],"In":[25],"this":[26],"study,":[27],"wild-type":[28,51],"mutant":[30],"forms":[31],"bovine":[33],"eNOS":[34,52,67,99,117,131,153,182,213,229],"were":[35],"overexpressed":[36],"baculovirus/Sf9":[39],"insect":[40],"cell":[41],"system":[42],"examined":[44],"for":[45,168,172,218],"interactions":[46,203],"with":[47,132,234,240],"membrane":[48,185,206,220],"phospholipids.":[49,98],"Purified":[50],"binds":[53],"to":[54,61,69,108,118,127,186,227],"pure":[55],"anionic":[56,89,205],"phospholipid":[57,63],"vesicles":[58,120],"but":[59,94,170],"not":[60,95,135,166],"neutral":[62,97],"vesicles,":[64],"demonstrating":[65],"that":[66,201],"attachment":[68],"lipid":[70],"bilayers":[71],"requires":[72],"electrostatic":[73,202],"as":[74,76],"well":[75],"hydrophobic":[77],"interactions.":[78],"Moreover,":[79],"catalytic":[80,230],"activity":[81,100,231],"the":[83,125,141,147,152,164,178,191,212,235,238],"enzyme":[84,106,126,165,192,219,239],"potently":[86],"inhibited":[87,104],"by":[88,96,137,232],"phospholipids,":[90],"notably":[91],"phosphatidylserine":[92],"(PS),":[93],"also":[102,171],"significantly":[103],"upon":[105],"binding":[107,123,161],"biological":[109],"membranes":[110],"isolated":[111],"from":[112,183],"cultured":[113],"cells.":[114,197],"Binding":[115],"PS":[119,133,173],"prevents":[121],"subsequent":[122],"CaM-Sepharose.":[128],"Interactions":[129],"are":[134,216],"affected":[136],"site-specific":[138],"mutation":[139,150],"myristic":[142],"acid":[143],"acceptor":[144],"site":[145],"enzyme.":[148],"Deletional":[149],"CaM-binding":[154,179,214],"domain,":[155],"however,":[156],"results":[157],"loss":[159],"capacity":[162],"only":[167],"CaM-Sepharose":[169],"vesicles.":[174],"Furthermore,":[175],"removal":[176],"domain":[180,215],"converts":[181],"cytosolic":[188],"protein":[189],"when":[190],"expressed":[194],"Sf9":[196],"These":[198],"data":[199],"demonstrate":[200],"between":[204],"phospholipids":[207],"basic":[209],"residues":[210],"association.":[221],"Membrane":[222],"association":[223],"can":[224],"thus":[225],"function":[226],"inhibit":[228],"interfering":[233],"interaction":[236],"calmodulin.":[241]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}