{"id":"https://openalex.org/W2094492452","doi":"https://doi.org/10.1371/journal.pone.0116955","title":"Microbial Community Composition and Diversity via 16S rRNA Gene Amplicons: Evaluating the Illumina Platform","display_name":"Microbial Community Composition and Diversity via 16S rRNA Gene Amplicons: Evaluating the Illumina Platform","publication_year":2015,"publication_date":"2015-02-03","ids":{"openalex":"https://openalex.org/W2094492452","doi":"https://doi.org/10.1371/journal.pone.0116955","mag":"2094492452","pmid":"https://pubmed.ncbi.nlm.nih.gov/25647581","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4315398"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0116955","doi":"https://doi.org/10.1371/journal.pone.0116955","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0116955","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0116955&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0116955&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014119589","display_name":"Lucas Sinclair","orcid":"https://orcid.org/0000-0003-4134-3571","observed_orcids":["https://orcid.org/0000-0003-4134-3571"]},"institutions":[{"id":"https://openalex.org/I123387679","display_name":"Uppsala University","ror":"https://ror.org/048a87296","country_code":"SE","type":"education","lineage":["https://openalex.org/I123387679"]},{"id":"https://openalex.org/I2800139495","display_name":"Science for Life Laboratory","ror":"https://ror.org/04ev03g22","country_code":"SE","type":"facility","lineage":["https://openalex.org/I2800139495"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Lucas Sinclair","raw_affiliation_strings":["Department of Ecology and Genetics, Limnology, and Science for Life Laboratory, Uppsala University, Uppsala, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ecology and Genetics, Limnology, and Science for Life Laboratory, Uppsala University, Uppsala, Sweden","institution_ids":["https://openalex.org/I123387679","https://openalex.org/I2800139495"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012561083","display_name":"Omneya Ahmed Osman","orcid":"https://orcid.org/0000-0002-1289-5070","observed_orcids":["https://orcid.org/0000-0002-1289-5070"]},"institutions":[{"id":"https://openalex.org/I123387679","display_name":"Uppsala University","ror":"https://ror.org/048a87296","country_code":"SE","type":"education","lineage":["https://openalex.org/I123387679"]},{"id":"https://openalex.org/I2800139495","display_name":"Science for Life Laboratory","ror":"https://ror.org/04ev03g22","country_code":"SE","type":"facility","lineage":["https://openalex.org/I2800139495"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Omneya Ahmed Osman","raw_affiliation_strings":["Department of Ecology and Genetics, Limnology, and Science for Life Laboratory, Uppsala University, Uppsala, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ecology and Genetics, Limnology, and Science for Life Laboratory, Uppsala University, Uppsala, Sweden","institution_ids":["https://openalex.org/I123387679","https://openalex.org/I2800139495"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057055718","display_name":"Stefan Bertilsson","orcid":"https://orcid.org/0000-0002-4265-1835","observed_orcids":["https://orcid.org/0000-0002-4265-1835"]},"institutions":[{"id":"https://openalex.org/I123387679","display_name":"Uppsala University","ror":"https://ror.org/048a87296","country_code":"SE","type":"education","lineage":["https://openalex.org/I123387679"]},{"id":"https://openalex.org/I2800139495","display_name":"Science for Life Laboratory","ror":"https://ror.org/04ev03g22","country_code":"SE","type":"facility","lineage":["https://openalex.org/I2800139495"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Stefan Bertilsson","raw_affiliation_strings":["Department of Ecology and Genetics, Limnology, and Science for Life Laboratory, Uppsala University, Uppsala, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ecology and Genetics, Limnology, and Science for Life Laboratory, Uppsala University, Uppsala, Sweden","institution_ids":["https://openalex.org/I123387679","https://openalex.org/I2800139495"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5010145648","display_name":"Alexander Eiler","orcid":"https://orcid.org/0000-0001-9916-9567","observed_orcids":["https://orcid.org/0000-0001-9916-9567"]},"institutions":[{"id":"https://openalex.org/I123387679","display_name":"Uppsala University","ror":"https://ror.org/048a87296","country_code":"SE","type":"education","lineage":["https://openalex.org/I123387679"]},{"id":"https://openalex.org/I2800139495","display_name":"Science for Life Laboratory","ror":"https://ror.org/04ev03g22","country_code":"SE","type":"facility","lineage":["https://openalex.org/I2800139495"]}],"countries":["SE"],"is_corresponding":true,"raw_author_name":"Alexander Eiler","raw_affiliation_strings":["Department of Ecology and Genetics, Limnology, and Science for Life Laboratory, Uppsala University, Uppsala, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ecology and Genetics, Limnology, and Science for Life Laboratory, Uppsala University, Uppsala, Sweden","institution_ids":["https://openalex.org/I123387679","https://openalex.org/I2800139495"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5010145648"],"corresponding_institution_ids":["https://openalex.org/I123387679","https://openalex.org/I2800139495"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":29.3904,"has_fulltext":true,"cited_by_count":380,"citation_normalized_percentile":{"value":0.99878063,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"10","issue":"2","first_page":"e0116955","last_page":"e0116955"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.7856000065803528,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.7856000065803528,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.17479999363422394,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.029600000008940697,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/16s-rrna-gene-amplicons","display_name":"16S rRNA gene amplicons","score":0.9169999957084656},{"id":"https://openalex.org/keywords/illumina-miseq","display_name":"Illumina MiSeq","score":0.8270000219345093},{"id":"https://openalex.org/keywords/sequencing-bias","display_name":"sequencing bias","score":0.6179999709129333},{"id":"https://openalex.org/keywords/alpha-diversity","display_name":"alpha diversity","score":0.7570000290870667},{"id":"https://openalex.org/keywords/beta-diversity","display_name":"beta diversity","score":1.0},{"id":"https://openalex.org/keywords/otu-clustering","display_name":"OTU clustering","score":0.753000020980835},{"id":"https://openalex.org/keywords/qiime","display_name":"QIIME","score":0.8529999852180481},{"id":"https://openalex.org/keywords/sediment-microbial-communities","display_name":"sediment microbial communities","score":0.7509999871253967},{"id":"https://openalex.org/keywords/dna-quantification","display_name":"DNA quantification","score":0.4650000035762787},{"id":"https://openalex.org/keywords/pcr-bias","display_name":"PCR bias","score":0.3889999985694885},{"id":"https://openalex.org/keywords/microbial-community-composition","display_name":"microbial community composition","score":0.5320000052452087}],"concepts":[{"id":"https://openalex.org/C2776841711","wikidata":"https://www.wikidata.org/wiki/Q856","display_name":"Barcode","level":2,"score":0.840471625328064},{"id":"https://openalex.org/C23913935","wikidata":"https://www.wikidata.org/wiki/Q2650881","display_name":"Alpha diversity","level":3,"score":0.7315527200698853},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.7258792519569397},{"id":"https://openalex.org/C125009961","wikidata":"https://www.wikidata.org/wiki/Q2342248","display_name":"Pyrosequencing","level":3,"score":0.7254419922828674},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5968285799026489},{"id":"https://openalex.org/C2993206177","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon sequencing","level":4,"score":0.5820140838623047},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5570799708366394},{"id":"https://openalex.org/C106337198","wikidata":"https://www.wikidata.org/wiki/Q13564012","display_name":"Illumina dye sequencing","level":4,"score":0.46147677302360535},{"id":"https://openalex.org/C25147867","wikidata":"https://www.wikidata.org/wiki/Q36039","display_name":"Beta diversity","level":3,"score":0.44979360699653625},{"id":"https://openalex.org/C151437367","wikidata":"https://www.wikidata.org/wiki/Q7884646","display_name":"UniFrac","level":4,"score":0.43093228340148926},{"id":"https://openalex.org/C73555534","wikidata":"https://www.wikidata.org/wiki/Q622825","display_name":"Cluster analysis","level":2,"score":0.4134778678417206},{"id":"https://openalex.org/C132917006","wikidata":"https://www.wikidata.org/wiki/Q5250436","display_name":"Deep sequencing","level":4,"score":0.4104498028755188},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3972972333431244},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.39687439799308777},{"id":"https://openalex.org/C42062724","wikidata":"https://www.wikidata.org/wiki/Q1209205","display_name":"16S ribosomal RNA","level":3,"score":0.34073859453201294},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.31886887550354004},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.2889149785041809},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.26498645544052124},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.26485106348991394},{"id":"https://openalex.org/C53565203","wikidata":"https://www.wikidata.org/wiki/Q17146659","display_name":"Species richness","level":2,"score":0.20791617035865784},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.16632705926895142},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.12518936395645142},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":true},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D044822","descriptor_name":"Biodiversity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":9,"locations":[{"id":"doi:10.1371/journal.pone.0116955","doi":"https://doi.org/10.1371/journal.pone.0116955","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0116955","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0116955&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},{"id":"doi:10.1101/010058","doi":"https://doi.org/10.1101/010058","is_oa":true,"landing_page_url":"https://doi.org/10.1101/010058","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2014/10/06/010058.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:europepmc.org:3292239","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4315398","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4315398","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4315398","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmid:25647581","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/25647581","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Evaluation Study"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.772.9983","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.772.9983","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/2f/19/pone.0116955.PMC4315398.pdf","raw_type":"text"},{"id":"pmh:oai:DiVA.org:uu-243557","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-243557","pdf_url":null,"source":{"id":"https://openalex.org/S4306402042","display_name":"Uppsala University Publications (Uppsala University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I123387679","host_organization_name":"Uppsala University","host_organization_lineage":["https://openalex.org/I123387679"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:doaj.org/article:d94f2673b5fa41d4a526b1a433b47e12","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/d94f2673b5fa41d4a526b1a433b47e12","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 10, Iss 2, p e0116955 (2015)","raw_type":"article"},{"id":"pmh:oai:figshare.com:article/1300441","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/dataset/_Microbial_Community_Composition_and_Diversity_via_16S_rRNA_Gene_Amplicons_Evaluating_the_Illumina_Platform_/1300441","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dataset"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0116955","doi":"https://doi.org/10.1371/journal.pone.0116955","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0116955","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0116955&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G7741240881","display_name":"Utforskning av bioteknologisk resurser fr\u00e5n soda sj\u00f6er","funder_award_id":"ICA10-0015","funder_id":"https://openalex.org/F4320320940","funder_display_name":"Stiftelsen f\u00f6r\u00a0Strategisk Forskning"}],"funders":[{"id":"https://openalex.org/F4320320940","display_name":"Stiftelsen f\u00f6r\u00a0Strategisk Forskning","ror":"https://ror.org/044wr7g58"},{"id":"https://openalex.org/F4320322581","display_name":"Vetenskapsr\u00e5det","ror":"https://ror.org/03zttf063"},{"id":"https://openalex.org/F4320324119","display_name":"Uppsala Universitet","ror":"https://ror.org/048a87296"},{"id":"https://openalex.org/F4320325677","display_name":"Science for Life Laboratory","ror":"https://ror.org/04ev03g22"},{"id":"https://openalex.org/F4320336376","display_name":"Uppsala Multidisciplinary Center for Advanced Computational Science","ror":"https://ror.org/0554ckj21"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2094492452.pdf","grobid_xml":"https://content.openalex.org/works/W2094492452.grobid-xml"},"referenced_works_count":50,"referenced_works":["https://openalex.org/W213734774","https://openalex.org/W1582334595","https://openalex.org/W1965423747","https://openalex.org/W1966689073","https://openalex.org/W1969435169","https://openalex.org/W1978198689","https://openalex.org/W1982396497","https://openalex.org/W1983690824","https://openalex.org/W2000072710","https://openalex.org/W2011127935","https://openalex.org/W2026677149","https://openalex.org/W2031611770","https://openalex.org/W2034285706","https://openalex.org/W2063984303","https://openalex.org/W2066452478","https://openalex.org/W2072970694","https://openalex.org/W2077592957","https://openalex.org/W2086021468","https://openalex.org/W2087671769","https://openalex.org/W2088833470","https://openalex.org/W2095132285","https://openalex.org/W2107581826","https://openalex.org/W2108718991","https://openalex.org/W2111971417","https://openalex.org/W2114766231","https://openalex.org/W2117457769","https://openalex.org/W2119284644","https://openalex.org/W2124351063","https://openalex.org/W2132088881","https://openalex.org/W2132548846","https://openalex.org/W2136879569","https://openalex.org/W2139448391","https://openalex.org/W2143980572","https://openalex.org/W2145166062","https://openalex.org/W2150914285","https://openalex.org/W2151772539","https://openalex.org/W2152885278","https://openalex.org/W2156417649","https://openalex.org/W2160209604","https://openalex.org/W2160681728","https://openalex.org/W2163295736","https://openalex.org/W2166171121","https://openalex.org/W2168104108","https://openalex.org/W2170024099","https://openalex.org/W2173924792","https://openalex.org/W2398350456","https://openalex.org/W2941307081","https://openalex.org/W3147310958","https://openalex.org/W3174993126","https://openalex.org/W4239131029"],"related_works":["https://openalex.org/W7099893659","https://openalex.org/W1986908904","https://openalex.org/W1963710004","https://openalex.org/W2117457769","https://openalex.org/W1512978277","https://openalex.org/W2607811538","https://openalex.org/W1966689073","https://openalex.org/W2949802999","https://openalex.org/W2560181459","https://openalex.org/W2520730496"],"abstract_inverted_index":{"As":[0],"new":[1,17],"sequencing":[2,44],"technologies":[3],"become":[4],"cheaper":[5],"and":[6,13,34,63,101,107,144,218,238,256,271,283,294],"older":[7],"ones":[8],"disappear,":[9],"laboratories":[10],"switch":[11],"vendors":[12],"platforms.":[14],"Validating":[15],"the":[16,32,46,69,72,90,93,119,169,177,184,196,211,219,228,252,279],"setups":[18],"is":[19],"a":[20,52,65,129,132,149,164,207],"crucial":[21],"part":[22],"of":[23,36,92,99,121,195,213,230,254,263],"conducting":[24],"rigorous":[25],"scientific":[26],"research.":[27],"Here":[28],"we":[29,88,111,156,205],"report":[30],"on":[31,45,153],"reliability":[33],"biases":[35],"performing":[37],"bacterial":[38],"16S":[39],"rRNA":[40],"gene":[41],"amplicon":[42],"paired-end":[43],"MiSeq":[47],"Illumina":[48,160,272],"platform.":[49],"We":[50,181],"designed":[51],"protocol":[53,161,282],"using":[54,243],"50":[55],"barcode":[56],"pairs":[57],"to":[58,67,97,115,193,227],"run":[59],"samples":[60,83],"in":[61,76,118,137,141,163,236,291],"parallel":[62],"coded":[64],"pipeline":[66],"process":[68],"data.":[70],"Sequencing":[71],"same":[73,280],"sediment":[74],"sample":[75],"248":[77],"replicates":[78],"as":[79,81],"well":[80,250],"70":[82],"from":[84],"alkaline":[85],"soda":[86],"lakes,":[87],"evaluated":[89],"performance":[91],"method":[94,151],"with":[95,148,168,215,260],"regards":[96],"estimates":[98,140,173],"alpha":[100,143,237,255],"beta":[102,145,239,257],"diversity.":[103,146],"Using":[104,127],"different":[105,139,201],"purification":[106],"DNA":[108],"quantification":[109],"procedures":[110],"always":[112],"found":[113,157],"up":[114],"5-fold":[116],"differences":[117],"yield":[120],"sequences":[122],"between":[123,176,210],"individually":[124],"barcodes":[125],"samples.":[126],"either":[128],"one-step":[130],"or":[131],"two-step":[133],"PCR":[134,281],"preparation":[135],"resulted":[136],"significantly":[138],"both":[142,244],"Comparing":[147],"previous":[150],"based":[152],"454":[154,270],"pyrosequencing,":[155],"that":[158,269],"our":[159],"performed":[162,249],"similar":[165],"manner":[166],"-":[167],"exception":[170],"for":[171,288],"evenness":[172],"where":[174],"correspondence":[175],"methods":[178],"was":[179],"low.":[180],"further":[182],"quantified":[183],"data":[185,274],"loss":[186],"at":[187],"every":[188],"processing":[189],"step":[190],"eventually":[191],"accumulating":[192],"50%":[194],"raw":[197],"reads.":[198],"When":[199],"evaluating":[200],"OTU":[202],"clustering":[203,245],"methods,":[204],"observed":[206],"stark":[208],"contrast":[209],"results":[212],"QIIME":[214],"default":[216],"settings":[217],"more":[220],"recent":[221],"UPARSE":[222],"algorithm":[223],"when":[224],"it":[225],"comes":[226],"number":[229],"OTUs":[231],"generated.":[232],"Still,":[233],"overall":[234],"trends":[235],"diversity":[240,258],"corresponded":[241],"highly":[242],"methods.":[246],"Our":[247],"procedure":[248],"considering":[251],"precisions":[253],"estimates,":[259],"insignificant":[261],"effects":[262],"individual":[264],"barcodes.":[265],"Comparative":[266],"analyses":[267],"suggest":[268],"sequence":[273],"can":[275],"be":[276],"combined":[277],"if":[278],"bioinformatic":[284],"workflows":[285],"are":[286],"used":[287],"describing":[289],"patterns":[290],"richness,":[292],"beta-diversity":[293],"taxonomic":[295],"composition.":[296]},"counts_by_year":[{"year":2026,"cited_by_count":19},{"year":2025,"cited_by_count":30},{"year":2024,"cited_by_count":35},{"year":2023,"cited_by_count":28},{"year":2022,"cited_by_count":32},{"year":2021,"cited_by_count":34},{"year":2020,"cited_by_count":50},{"year":2019,"cited_by_count":42},{"year":2018,"cited_by_count":34},{"year":2017,"cited_by_count":45},{"year":2016,"cited_by_count":22},{"year":2015,"cited_by_count":9}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}