{"id":"https://openalex.org/W2093769812","doi":"https://doi.org/10.1158/1538-7445.am2011-4553","title":"Abstract 4553: G\u03b2\u03b3 subunits inhibit Epac-induced melanoma cell migration","display_name":"Abstract 4553: G\u03b2\u03b3 subunits inhibit Epac-induced melanoma cell migration","publication_year":2011,"publication_date":"2011-04-01","ids":{"openalex":"https://openalex.org/W2093769812","doi":"https://doi.org/10.1158/1538-7445.am2011-4553","mag":"2093769812"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2011-4553","doi":"https://doi.org/10.1158/1538-7445.am2011-4553","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2011-4553","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110216524","display_name":"Erdene Baljinnyam","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4402554120","display_name":"Rutgers New Jersey Medical School","ror":"https://ror.org/014ye1258","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142","https://openalex.org/I4390039302","https://openalex.org/I4402554120"]},{"id":"https://openalex.org/I4405286694","display_name":"University of Medicine and Dentistry of New Jersey","ror":"https://ror.org/02tvvmk84","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142","https://openalex.org/I4405286694"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Erdene Baljinnyam","raw_affiliation_strings":["1Department of Cell Biology and Molecular Medicine, New Jersey Medical School-University of Medicine and Dentistry of New Jersey, Newark, NJ"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Department of Cell Biology and Molecular Medicine, New Jersey Medical School-University of Medicine and Dentistry of New Jersey, Newark, NJ","institution_ids":["https://openalex.org/I4402554120","https://openalex.org/I4405286694"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018686340","display_name":"Masanari Umemura","orcid":"https://orcid.org/0000-0002-1812-5720","observed_orcids":["https://orcid.org/0000-0002-1812-5720"]},"institutions":[{"id":"https://openalex.org/I4402554120","display_name":"Rutgers New Jersey Medical School","ror":"https://ror.org/014ye1258","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142","https://openalex.org/I4390039302","https://openalex.org/I4402554120"]},{"id":"https://openalex.org/I4405286694","display_name":"University of Medicine and Dentistry of New Jersey","ror":"https://ror.org/02tvvmk84","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142","https://openalex.org/I4405286694"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Masanari Umemura","raw_affiliation_strings":["1Department of Cell Biology and Molecular Medicine, New Jersey Medical School-University of Medicine and Dentistry of New Jersey, Newark, NJ"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Department of Cell Biology and Molecular Medicine, New Jersey Medical School-University of Medicine and Dentistry of New Jersey, Newark, NJ","institution_ids":["https://openalex.org/I4402554120","https://openalex.org/I4405286694"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084773942","display_name":"Lai\u2010Hua Xie","orcid":"https://orcid.org/0000-0003-2600-2269","observed_orcids":["https://orcid.org/0000-0003-2600-2269"]},"institutions":[{"id":"https://openalex.org/I4402554120","display_name":"Rutgers New Jersey Medical School","ror":"https://ror.org/014ye1258","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142","https://openalex.org/I4390039302","https://openalex.org/I4402554120"]},{"id":"https://openalex.org/I4405286694","display_name":"University of Medicine and Dentistry of New Jersey","ror":"https://ror.org/02tvvmk84","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142","https://openalex.org/I4405286694"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lai-Hua Xie","raw_affiliation_strings":["1Department of Cell Biology and Molecular Medicine, New Jersey Medical School-University of Medicine and Dentistry of New Jersey, Newark, NJ"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Department of Cell Biology and Molecular Medicine, New Jersey Medical School-University of Medicine and Dentistry of New Jersey, Newark, NJ","institution_ids":["https://openalex.org/I4402554120","https://openalex.org/I4405286694"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048608190","display_name":"Martha C. Nowycky","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4402554120","display_name":"Rutgers New Jersey Medical School","ror":"https://ror.org/014ye1258","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142","https://openalex.org/I4390039302","https://openalex.org/I4402554120"]},{"id":"https://openalex.org/I4405286694","display_name":"University of Medicine and Dentistry of New Jersey","ror":"https://ror.org/02tvvmk84","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142","https://openalex.org/I4405286694"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Martha Nowycky","raw_affiliation_strings":["2Department of Pharmacology and Physiology, New Jersey Medical School-University of Medicine and Dentistry of New Jersey, Newark, NJ"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Department of Pharmacology and Physiology, New Jersey Medical School-University of Medicine and Dentistry of New Jersey, Newark, NJ","institution_ids":["https://openalex.org/I4402554120","https://openalex.org/I4405286694"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5016576365","display_name":"Kousaku Iwatsubo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kousaku Iwatsubo","raw_affiliation_strings":["3Department of Cardiovascular Research Institute, Yokohama City University School of Medicine, Yokohama, Japan, Newark, NJ"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3Department of Cardiovascular Research Institute, Yokohama City University School of Medicine, Yokohama, Japan, Newark, NJ","institution_ids":["https://openalex.org/I89630735"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"71","issue":"8_Supplement","first_page":"4553","last_page":"4553"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.8353999853134155,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.8353999853134155,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.0763000026345253,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13415","display_name":"Calcium signaling and nucleotide metabolism","score":0.0414000004529953,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gbetagamma-subunits","display_name":"G\u03b2\u03b3 subunits","score":0.9359999895095825},{"id":"https://openalex.org/keywords/epac1","display_name":"Epac1","score":0.9760000109672546},{"id":"https://openalex.org/keywords/melanoma-cell-migration","display_name":"melanoma cell migration","score":0.875},{"id":"https://openalex.org/keywords/calcium-signaling","display_name":"calcium signaling","score":0.7680000066757202},{"id":"https://openalex.org/keywords/camp-signaling","display_name":"cAMP signaling","score":0.6159999966621399},{"id":"https://openalex.org/keywords/extracellular-calcium-influx","display_name":"extracellular calcium influx","score":0.7070000171661377},{"id":"https://openalex.org/keywords/er-calcium-release","display_name":"endoplasmic reticulum calcium release","score":0.8569999933242798},{"id":"https://openalex.org/keywords/beta-adrenergic-receptor-kinase","display_name":"beta-adrenergic receptor kinase","score":0.7789999842643738},{"id":"https://openalex.org/keywords/gdp-beta-s","display_name":"GDP beta S","score":0.6290000081062317},{"id":"https://openalex.org/keywords/metastatic-melanoma","display_name":"metastatic melanoma","score":0.7269999980926514},{"id":"https://openalex.org/keywords/cell-migration-inhibition","display_name":"cell migration inhibition","score":0.33399999141693115}],"concepts":[{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.7173633575439453},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.5848932266235352},{"id":"https://openalex.org/C98539663","wikidata":"https://www.wikidata.org/wiki/Q220599","display_name":"Cytosol","level":3,"score":0.5581386089324951},{"id":"https://openalex.org/C137738243","wikidata":"https://www.wikidata.org/wiki/Q189092","display_name":"Cell migration","level":3,"score":0.5422545075416565},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.4897923469543457},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.47685539722442627},{"id":"https://openalex.org/C169822122","wikidata":"https://www.wikidata.org/wiki/Q230187","display_name":"Crosstalk","level":2,"score":0.4725339710712433},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.47186487913131714},{"id":"https://openalex.org/C2777658100","wikidata":"https://www.wikidata.org/wiki/Q180614","display_name":"Melanoma","level":2,"score":0.4578685760498047},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.4552941620349884},{"id":"https://openalex.org/C158617107","wikidata":"https://www.wikidata.org/wiki/Q79927","display_name":"Endoplasmic reticulum","level":2,"score":0.45245254039764404},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.44343188405036926},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.426655650138855},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3992033004760742},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.3901011645793915},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.3762584924697876},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3373551070690155},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.2397308349609375},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.21289175748825073},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0615936815738678},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2011-4553","doi":"https://doi.org/10.1158/1538-7445.am2011-4553","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2011-4553","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2156275481","https://openalex.org/W7100270518","https://openalex.org/W2322832366","https://openalex.org/W2144517964","https://openalex.org/W2260733730","https://openalex.org/W2329166788","https://openalex.org/W2018252424","https://openalex.org/W2401521092","https://openalex.org/W4285388829","https://openalex.org/W2887229828"],"abstract_inverted_index":{"Abstract":[0],"Background:":[1],"Recently":[2],"we":[3,64],"reported":[4],"that":[5,66,175,245,255,322],"activation":[6,41],"of":[7,42,49,70,83,136,171,213,222,230,266,296,352,357,376,394,399],"Epac1,":[8],"an":[9,36,202,234,325,335],"Exchange":[10],"protein":[11],"activated":[12],"by":[13,169,277],"cAMP,":[14],"increases":[15],"melanoma":[16,57,90,160,330],"cell":[17,85,91,161,270,273,292,303,315,331,348,382],"migration":[18,55,117,274,316,383],"via":[19],"Ca":[20],"2+":[21],"release":[22],"from":[23,180,369],"the":[24,47,121,264,355,395,400],"endoplasmic":[25],"reticulum":[26],"(ER).":[27],"G-protein":[28,43],"subunits":[29,127,300],"(G\u03b2\u03b3)":[30],"are":[31],"known":[32],"to":[33,206],"act":[34],"as":[35],"independent":[37],"signaling":[38,53,72,359],"molecule":[39],"upon":[40],"coupled":[44],"receptor.":[45],"However,":[46],"role":[48],"G\u03b2\u03b3":[50,74,151,176,231,256,267,323,361,365],"in":[51,56,77,81,102,158,354,374,384],"Ca2+":[52,71,106,165,178,227,250,259,345,358,367,379],"and":[54,75,98,125,215,298,347,362,381],"has":[58,324],"not":[59,190],"been":[60],"well":[61],"studied.":[62],"Here":[63],"report":[65],"there":[67],"is":[68,218],"crosstalk":[69,356],"between":[73,360],"Epac1":[76],"melanoma,":[78],"which":[79,142,217,372],"results":[80,373],"inhibition":[82],"Epac-induced":[84,258,269,272,291,329,344,377],"migration.":[86,271,293,304,332],"Methods:":[87],"Human":[88],"metastatic":[89],"lines,":[92],"SK-Mel-2,":[93],"SK-Mel-24,":[94],"SK-Mel-187,":[95],"c8161,":[96],"WM1552C,":[97],"WM1361A,":[99],"were":[100,128,185],"used":[101],"this":[103],"study.":[104],"Intracellular":[105],"level":[107],"was":[108,118,145,167,275,309,317],"measured":[109],"with":[110,120,130,147,187,191,232],"Fura-2AM":[111],"and/or":[112],"Fluo-4AM":[113],"fluorescent":[114],"dyes.":[115],"Cell":[116],"examined":[119,263],"Boyden":[122],"chambers.":[123],"G\u03b21":[124,297],"G\u03b32":[126,299],"overexpressed":[129,146],"plasmid":[131],"harboring":[132],"each":[133],"subunit.":[134],"C-terminus":[135],"\u03b2":[137],"adrenergic":[138],"receptor":[139],"kinase":[140],"(\u03b2ARK-CT),":[141],"inhibits":[143,257],"G\u03b2\u03b3,":[144,223,237,247],"adenovirus.":[148],"Results:":[149],"A":[150],"-activating":[152],"peptide,":[153],"mSIRK,":[154,188],"increased":[155],"cytosolic":[156,208,378],"Ca2+,":[157,173],"all":[159],"lines.":[162],"mSIRK":[163,192,196],"induced":[164],"entry":[166,368],"inhibited":[168,225,290,301],"depletion":[170],"extracellular":[172,181,370],"suggesting":[174,350],"activates":[177],"influx":[179],"space.":[182],"When":[183],"cells":[184],"pretreated":[186],"but":[189],"(L9A),":[193],"a":[194,219,242,284],"non-functional":[195],"analogue,":[197,288],"8-(4-Methoxyphenylthio)-2\u2032-O-methyladenosine-":[198],"3\u2032,5\u2032-cyclic":[199],"monophosphate":[200],"(8-pMeOPT),":[201],"Epac-specific":[203],"agonist,":[204],"failed":[205],"increase":[207],"Ca2+.":[209],"In":[210],"addition,":[211],"co-overexpression":[212,295],"\u03b21":[214],"\u03b2\u03b32,":[216],"major":[220],"combination":[221],"also":[224,289],"8-pMeOPT-induced":[226,249],"elevation.":[228,251],"Inhibition":[229],"\u03b2ARK-CT,":[233],"inhibiting-peptide":[235],"for":[236,337,403],"or":[238],"guanosine":[239],"5\u2032-O-(2-thiodiphosphate)":[240],"(GDP\u03b2S),":[241],"GDP":[243],"analogue":[244],"inactivates":[246],"recovered":[248],"These":[252,319],"data":[253,320],"suggested":[254,321],"release.":[260],"We":[261],"then":[262],"effect":[265,313,327],"on":[268,314,328,343],"decreased":[276],"mSIRK.":[278],"Gp(CH)2pp":[279],"(Guanosine":[280],"5\u2032,":[281],"\u03b1-\u03b2-methylene":[282],"triphosphate),":[283],"constitutively":[285],"active":[286],"GTP":[287],"Further,":[294],"Epac1-induced":[302],"By":[305],"contrast,":[306],"when":[307],"\u03b2ARK-CT":[308],"overexpressed,":[310],"mSIRK's":[311,340],"inhibitory":[312,326,341],"negated.":[318],"Finally,":[333],"W-5,":[334],"inhibitor":[336],"calmodulin,":[338],"negated":[339],"effects":[342],"elevation,":[346],"migration,":[349],"involvement":[351],"calmodulin":[353],"Epac.":[363],"Conclusion:":[364],"evokes":[366],"space,":[371],"suppression":[375],"elevation":[380],"melanoma.":[385],"Citation":[386],"Format:":[387],"{Authors}.":[388],"{Abstract":[389],"title}":[390],"[abstract].":[391],"In:":[392],"Proceedings":[393],"102nd":[396],"Annual":[397],"Meeting":[398],"American":[401],"Association":[402],"Cancer":[404,414],"Research;":[405],"2011":[406],"Apr":[407],"2-6;":[408],"Orlando,":[409],"FL.":[410],"Philadelphia":[411],"(PA):":[412],"AACR;":[413],"Res":[415],"2011;71(8":[416],"Suppl):Abstract":[417],"nr":[418],"4553.":[419],"doi:10.1158/1538-7445.AM2011-4553":[420]},"counts_by_year":[],"updated_date":"2026-10-24T09:50:30.924529","created_date":"2016-06-24T00:00:00"}