{"id":"https://openalex.org/W2092503176","doi":"https://doi.org/10.1074/jbc.m000491200","title":"Interactions of the DNA Ligase IV-XRCC4 Complex with DNA Ends and the DNA-dependent Protein Kinase","display_name":"Interactions of the DNA Ligase IV-XRCC4 Complex with DNA Ends and the DNA-dependent Protein Kinase","publication_year":2000,"publication_date":"2000-08-01","ids":{"openalex":"https://openalex.org/W2092503176","doi":"https://doi.org/10.1074/jbc.m000491200","mag":"2092503176","pmid":"https://pubmed.ncbi.nlm.nih.gov/10854421"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m000491200","doi":"https://doi.org/10.1074/jbc.m000491200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m000491200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.m000491200","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100411137","display_name":"Ling Chen","orcid":"https://orcid.org/0000-0003-1485-1626","observed_orcids":["https://orcid.org/0000-0003-1485-1626","https://orcid.org/0000-0002-0150-4433","https://orcid.org/0000-0003-2336-2779"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ling Chen","raw_affiliation_strings":["Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245, USA","From the Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245, USA","institution_ids":["https://openalex.org/I165951966"]},{"raw_affiliation_string":"From the Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110087302","display_name":"Kelly M. Trujillo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kelly Trujillo","raw_affiliation_strings":["From the Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090899284","display_name":"Patrick Sung","orcid":"https://orcid.org/0000-0003-1396-9040","observed_orcids":["https://orcid.org/0000-0003-1396-9040"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Patrick Sung","raw_affiliation_strings":["From the Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5051816777","display_name":"Alan E. Tomkinson","orcid":"https://orcid.org/0000-0002-2671-1711","observed_orcids":["https://orcid.org/0000-0002-2671-1711"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Alan E. Tomkinson","raw_affiliation_strings":["From the Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245","institution_ids":["https://openalex.org/I165951966"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5051816777"],"corresponding_institution_ids":["https://openalex.org/I165951966"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":5.6656,"has_fulltext":false,"cited_by_count":235,"citation_normalized_percentile":{"value":0.97138485,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"275","issue":"34","first_page":"26196","last_page":"26205"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-dependent-protein-kinase","display_name":"DNA-dependent protein kinase","score":0.9470000267028809},{"id":"https://openalex.org/keywords/dna-pk-catalytic-subunit","display_name":"DNA-PK catalytic subunit","score":0.8740000128746033},{"id":"https://openalex.org/keywords/ku-protein","display_name":"Ku protein","score":0.9620000123977661},{"id":"https://openalex.org/keywords/non-homologous-end-joining","display_name":"non-homologous end joining","score":0.9070000052452087},{"id":"https://openalex.org/keywords/double-strand-break-repair","display_name":"DNA double-strand break repair","score":0.9879999756813049},{"id":"https://openalex.org/keywords/v-d-j-recombination","display_name":"V(D)J recombination","score":0.9750000238418579},{"id":"https://openalex.org/keywords/dna-end-binding","display_name":"DNA end binding","score":0.7720000147819519},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.8289999961853027}],"concepts":[{"id":"https://openalex.org/C175114707","wikidata":"https://www.wikidata.org/wiki/Q408483","display_name":"DNA ligase","level":3,"score":0.8321032524108887},{"id":"https://openalex.org/C145803527","wikidata":"https://www.wikidata.org/wiki/Q18032396","display_name":"DNA repair protein XRCC4","level":5,"score":0.5775434970855713},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.47273221611976624},{"id":"https://openalex.org/C116988904","wikidata":"https://www.wikidata.org/wiki/Q18038809","display_name":"DNA polymerase mu","level":5,"score":0.45212414860725403},{"id":"https://openalex.org/C140248907","wikidata":"https://www.wikidata.org/wiki/Q17913449","display_name":"DDB1","level":5,"score":0.4466816186904907},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4045906066894531},{"id":"https://openalex.org/C134935766","wikidata":"https://www.wikidata.org/wiki/Q210538","display_name":"DNA repair","level":3,"score":0.4023205637931824},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38623929023742676},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36012744903564453},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.35993367433547974},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.32830649614334106},{"id":"https://openalex.org/C134459356","wikidata":"https://www.wikidata.org/wiki/Q4274835","display_name":"Ubiquitin ligase","level":4,"score":0.3069531321525574},{"id":"https://openalex.org/C58167604","wikidata":"https://www.wikidata.org/wiki/Q5121654","display_name":"Circular bacterial chromosome","level":4,"score":0.2256556749343872},{"id":"https://openalex.org/C2756471","wikidata":"https://www.wikidata.org/wiki/Q206286","display_name":"DNA polymerase","level":3,"score":0.1842328906059265},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1359533965587616},{"id":"https://openalex.org/C104451858","wikidata":"https://www.wikidata.org/wiki/Q2714458","display_name":"Nucleotide excision repair","level":4,"score":0.10617712140083313},{"id":"https://openalex.org/C25602115","wikidata":"https://www.wikidata.org/wiki/Q407111","display_name":"Ubiquitin","level":3,"score":0.10481506586074829}],"mesh":[{"descriptor_ui":"D000072481","descriptor_name":"DNA Ligase ATP","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004260","descriptor_name":"DNA Repair","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004268","descriptor_name":"DNA-Binding Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009687","descriptor_name":"Nuclear Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011088","descriptor_name":"DNA Ligases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017346","descriptor_name":"Protein Serine-Threonine Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018411","descriptor_name":"Spodoptera","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046911","descriptor_name":"Macromolecular Substances","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051747","descriptor_name":"DNA-Activated Protein Kinase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m000491200","doi":"https://doi.org/10.1074/jbc.m000491200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m000491200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:10854421","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10854421","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m000491200","doi":"https://doi.org/10.1074/jbc.m000491200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m000491200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4687494457","display_name":null,"funder_award_id":"GM 47251","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G477439269","display_name":null,"funder_award_id":"ES07061","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"},{"id":"https://openalex.org/G9000856387","display_name":"Mechanisms of DNA Double-strand Break End Resection and Repair Pathway Choice","funder_award_id":"R01ES007061","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337361","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":47,"referenced_works":["https://openalex.org/W1482342775","https://openalex.org/W1486192115","https://openalex.org/W1491106772","https://openalex.org/W1529195725","https://openalex.org/W1532833901","https://openalex.org/W1550791622","https://openalex.org/W1587492859","https://openalex.org/W1604391934","https://openalex.org/W1627029737","https://openalex.org/W1660787335","https://openalex.org/W1785654530","https://openalex.org/W1956055380","https://openalex.org/W1965446460","https://openalex.org/W1985680698","https://openalex.org/W1989299971","https://openalex.org/W2003196136","https://openalex.org/W2007437374","https://openalex.org/W2007635472","https://openalex.org/W2007897155","https://openalex.org/W2011320725","https://openalex.org/W2015090136","https://openalex.org/W2020633581","https://openalex.org/W2026815183","https://openalex.org/W2034746516","https://openalex.org/W2038875604","https://openalex.org/W2041413912","https://openalex.org/W2042645510","https://openalex.org/W2061357246","https://openalex.org/W2068176376","https://openalex.org/W2070369325","https://openalex.org/W2071695004","https://openalex.org/W2077634990","https://openalex.org/W2082910780","https://openalex.org/W2083311265","https://openalex.org/W2083326500","https://openalex.org/W2084919692","https://openalex.org/W2088474558","https://openalex.org/W2089138444","https://openalex.org/W2099458703","https://openalex.org/W2111924246","https://openalex.org/W2114322342","https://openalex.org/W2120143131","https://openalex.org/W2127173305","https://openalex.org/W2155338983","https://openalex.org/W2157451489","https://openalex.org/W2163658254","https://openalex.org/W2432429035"],"related_works":["https://openalex.org/W1963613246","https://openalex.org/W2092920840","https://openalex.org/W2013580891","https://openalex.org/W7214215354","https://openalex.org/W2158099110","https://openalex.org/W2171672464","https://openalex.org/W2003196136","https://openalex.org/W1964171776","https://openalex.org/W2088474558","https://openalex.org/W2147022500"],"abstract_inverted_index":{"The":[0],"DNA-dependent":[1],"protein":[2,34,88],"kinase":[3],"(DNA-PK),":[4],"consisting":[5],"of":[6,25,42,63,118,133,154,158,192],"Ku":[7,89,112,170],"and":[8,14,67,111,125],"the":[9,15,23,40,53,61,85,116,131,152,156,159,190],"DNA-PK":[10],"catalytic":[11],"subunit":[12],"(DNA-PKcs),":[13],"DNA":[16,26,54,65,77,86,91,94,108,120,122,134,148,164,187,193],"ligase":[17,55,95,109,123,149,165],"IV-XRCC4":[18,56,110,124,166],"complex":[19,57,102],"function":[20],"together":[21,75],"in":[22,46],"repair":[24],"double-strand":[27],"breaks":[28],"by":[29,93,147,163],"non-homologous":[30],"end":[31],"joining.":[32],"These":[33],"complexes":[35,129,184],"are":[36],"also":[37,127],"required":[38],"for":[39],"completion":[41],"V(D)J":[43],"recombination":[44],"events":[45,161],"immune":[47],"cells.":[48],"Here":[49],"we":[50],"demonstrate":[51],"that":[52,182],"binds":[58],"specifically":[59],"to":[60,105,115],"ends":[62,117,132,188,194],"duplex":[64,76,119],"molecules":[66,78],"can":[68],"act":[69],"as":[70],"a":[71],"bridging":[72],"factor,":[73],"linking":[74],"with":[79],"complementary":[80],"but":[81,136,174],"non-ligatable":[82],"ends.":[83],"Although":[84],"end-binding":[87],"inhibited":[90,171],"joining":[92,160],"IV-XRCC4,":[96],"it":[97],"did":[98,138],"not":[99,139],"prevent":[100],"this":[101],"from":[103],"binding":[104],"DNA.":[106],"Instead,":[107],"bound":[113],"simultaneously":[114],"molecules.":[121],"DNA-PKcs":[126,137,143,175],"formed":[128,185],"at":[130,186],"molecules,":[135],"inhibit":[140],"ligation.":[141,179,201],"Interestingly,":[142],"stimulated":[144,177],"intermolecular":[145,168,200],"ligation":[146],"IV-XRCC4.":[150],"In":[151],"presence":[153],"DNA-PK,":[155],"majority":[157],"catalyzed":[162],"were":[167],"because":[169],"intramolecular":[172,178],"ligation,":[173],"still":[176],"We":[180],"suggest":[181],"DNA-PKcs-containing":[183],"enhance":[189],"association":[191],"via":[195],"protein-protein":[196],"interactions,":[197],"thereby":[198],"stimulating":[199]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":9},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":6},{"year":2014,"cited_by_count":9},{"year":2013,"cited_by_count":9},{"year":2012,"cited_by_count":13}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
