{"id":"https://openalex.org/W2092500663","doi":"https://doi.org/10.1002/cbic.200800704","title":"On\u2010Chip Fragment\u2010Based Approach for Discovery of High\u2010Affinity Bivalent Inhibitors","display_name":"On\u2010Chip Fragment\u2010Based Approach for Discovery of High\u2010Affinity Bivalent Inhibitors","publication_year":2009,"publication_date":"2009-02-18","ids":{"openalex":"https://openalex.org/W2092500663","doi":"https://doi.org/10.1002/cbic.200800704","mag":"2092500663","pmid":"https://pubmed.ncbi.nlm.nih.gov/19226504"},"language":"en","primary_location":{"id":"doi:10.1002/cbic.200800704","doi":"https://doi.org/10.1002/cbic.200800704","is_oa":false,"landing_page_url":"https://doi.org/10.1002/cbic.200800704","pdf_url":null,"source":{"id":"https://openalex.org/S154285657","display_name":"ChemBioChem","issn_l":"1439-4227","issn":["1439-4227","1439-7633"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ChemBioChem","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060177022","display_name":"Isao Miyazaki","orcid":"https://orcid.org/0009-0008-3742-1243","observed_orcids":["https://orcid.org/0009-0008-3742-1243"]},"institutions":[{"id":"https://openalex.org/I4210110652","display_name":"RIKEN","ror":"https://ror.org/01sjwvz98","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210110652"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Isao Miyazaki","raw_affiliation_strings":["Antibiotics Laboratory and Chemical Biology Department, RIKEN, Wako, Saitama 351-0198, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Antibiotics Laboratory and Chemical Biology Department, RIKEN, Wako, Saitama 351-0198, Japan","institution_ids":["https://openalex.org/I4210110652"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035440904","display_name":"Siro Simizu","orcid":"https://orcid.org/0000-0003-2949-2427","observed_orcids":["https://orcid.org/0000-0003-2949-2427"]},"institutions":[{"id":"https://openalex.org/I4210110652","display_name":"RIKEN","ror":"https://ror.org/01sjwvz98","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210110652"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Siro Simizu","raw_affiliation_strings":["Antibiotics Laboratory and Chemical Biology Department, RIKEN, Wako, Saitama 351\u20100198 (Japan), Fax: (+81)\u200948\u2010462\u20104669"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Antibiotics Laboratory and Chemical Biology Department, RIKEN, Wako, Saitama 351\u20100198 (Japan), Fax: (+81)\u200948\u2010462\u20104669","institution_ids":["https://openalex.org/I4210110652"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112246779","display_name":"Keisuke Ishida","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210110652","display_name":"RIKEN","ror":"https://ror.org/01sjwvz98","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210110652"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Keisuke Ishida","raw_affiliation_strings":["Antibiotics Laboratory and Chemical Biology Department, RIKEN, Wako, Saitama 351\u20100198 (Japan), Fax: (+81)\u200948\u2010462\u20104669"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Antibiotics Laboratory and Chemical Biology Department, RIKEN, Wako, Saitama 351\u20100198 (Japan), Fax: (+81)\u200948\u2010462\u20104669","institution_ids":["https://openalex.org/I4210110652"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5054922975","display_name":"Hiroyuki Osada","orcid":"https://orcid.org/0000-0002-3606-4925","observed_orcids":["https://orcid.org/0000-0002-3606-4925"]},"institutions":[{"id":"https://openalex.org/I4210110652","display_name":"RIKEN","ror":"https://ror.org/01sjwvz98","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210110652"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroyuki Osada","raw_affiliation_strings":["Antibiotics Laboratory and Chemical Biology Department, RIKEN, Wako, Saitama 351\u20100198 (Japan), Fax: (+81)\u200948\u2010462\u20104669"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Antibiotics Laboratory and Chemical Biology Department, RIKEN, Wako, Saitama 351\u20100198 (Japan), Fax: (+81)\u200948\u2010462\u20104669","institution_ids":["https://openalex.org/I4210110652"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210110652"],"apc_list":{"value":4430,"currency":"USD","value_usd":4430},"apc_paid":null,"fwci":0.9655,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.70072501,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":93},"biblio":{"volume":"10","issue":"5","first_page":"838","last_page":"843"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.4844000041484833,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.4844000041484833,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.4065000116825104,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.031199999153614044,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bivalent-inhibitors","display_name":"bivalent inhibitors","score":0.9909999966621399},{"id":"https://openalex.org/keywords/covalent-immobilization","display_name":"covalent immobilization","score":0.7490000128746033},{"id":"https://openalex.org/keywords/high-affinity-ligands","display_name":"high-affinity ligands","score":0.9229999780654907},{"id":"https://openalex.org/keywords/protein-ligand-binding","display_name":"protein-ligand binding","score":0.7149999737739563}],"concepts":[{"id":"https://openalex.org/C2776620830","wikidata":"https://www.wikidata.org/wiki/Q4919211","display_name":"Bivalent (engine)","level":3,"score":0.7734901905059814},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.7413034439086914},{"id":"https://openalex.org/C2776235265","wikidata":"https://www.wikidata.org/wiki/Q18392052","display_name":"Fragment (logic)","level":2,"score":0.6511583924293518},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.6469125747680664},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5906738042831421},{"id":"https://openalex.org/C2780430158","wikidata":"https://www.wikidata.org/wiki/Q7685902","display_name":"Target protein","level":3,"score":0.44229254126548767},{"id":"https://openalex.org/C74187038","wikidata":"https://www.wikidata.org/wiki/Q1418791","display_name":"Drug discovery","level":2,"score":0.42187392711639404},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.3520492911338806},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.34226319193840027},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.21359142661094666},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.21006327867507935},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.16871154308319092},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.16389289498329163},{"id":"https://openalex.org/C11413529","wikidata":"https://www.wikidata.org/wiki/Q8366","display_name":"Algorithm","level":1,"score":0.09919846057891846},{"id":"https://openalex.org/C544153396","wikidata":"https://www.wikidata.org/wiki/Q11426","display_name":"Metal","level":2,"score":0.06897196173667908},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007166","descriptor_name":"Immunosuppressive Agents","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007166","descriptor_name":"Immunosuppressive Agents","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015394","descriptor_name":"Molecular Structure","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016559","descriptor_name":"Tacrolimus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016559","descriptor_name":"Tacrolimus","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D022061","descriptor_name":"Tacrolimus Binding Protein 1A","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D022061","descriptor_name":"Tacrolimus Binding Protein 1A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D040081","descriptor_name":"Protein Array Analysis","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":true},{"descriptor_ui":"D040081","descriptor_name":"Protein Array Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D055808","descriptor_name":"Drug Discovery","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/cbic.200800704","doi":"https://doi.org/10.1002/cbic.200800704","is_oa":false,"landing_page_url":"https://doi.org/10.1002/cbic.200800704","pdf_url":null,"source":{"id":"https://openalex.org/S154285657","display_name":"ChemBioChem","issn_l":"1439-4227","issn":["1439-4227","1439-7633"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ChemBioChem","raw_type":"journal-article"},{"id":"pmid:19226504","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19226504","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chembiochem : a European journal of chemical biology","raw_type":"Evaluation Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":47,"referenced_works":["https://openalex.org/W1502177285","https://openalex.org/W1522908541","https://openalex.org/W1967999935","https://openalex.org/W1972091733","https://openalex.org/W1977771641","https://openalex.org/W1979360726","https://openalex.org/W1984596640","https://openalex.org/W1992763719","https://openalex.org/W1996100382","https://openalex.org/W2000304827","https://openalex.org/W2002776639","https://openalex.org/W2003932771","https://openalex.org/W2003950014","https://openalex.org/W2009558341","https://openalex.org/W2046251254","https://openalex.org/W2046258407","https://openalex.org/W2047178318","https://openalex.org/W2050535564","https://openalex.org/W2060900964","https://openalex.org/W2065633704","https://openalex.org/W2067175433","https://openalex.org/W2080328450","https://openalex.org/W2082570311","https://openalex.org/W2086552937","https://openalex.org/W2088267152","https://openalex.org/W2089218912","https://openalex.org/W2090507519","https://openalex.org/W2102593999","https://openalex.org/W2104192287","https://openalex.org/W2106006920","https://openalex.org/W2116519993","https://openalex.org/W2123342482","https://openalex.org/W2132055541","https://openalex.org/W2134503191","https://openalex.org/W2138958920","https://openalex.org/W2143951116","https://openalex.org/W2149863287","https://openalex.org/W2150804079","https://openalex.org/W2159701165","https://openalex.org/W2162703779","https://openalex.org/W2743535258","https://openalex.org/W2950124390","https://openalex.org/W3100743833","https://openalex.org/W4205361388","https://openalex.org/W4211118618","https://openalex.org/W4233513905","https://openalex.org/W4244551340"],"related_works":["https://openalex.org/W1983611229","https://openalex.org/W3128963846","https://openalex.org/W4246863339","https://openalex.org/W3182620279","https://openalex.org/W3083833598","https://openalex.org/W3097389505","https://openalex.org/W40038298","https://openalex.org/W2944392659","https://openalex.org/W3144955865","https://openalex.org/W2028816469"],"abstract_inverted_index":{"Abstract":[0],"Covalent":[1],"bonds":[2],"not":[3,47],"required:":[4],"We":[5],"describe":[6],"a":[7,38],"novel":[8],"approach":[9,68],"in":[10],"which":[11],"the":[12,49,65],"concepts":[13],"of":[14,30],"fragment\u2010based":[15,67],"ligand":[16],"discovery":[17],"are":[18,62],"combined":[19],"with":[20],"chemical":[21],"array":[22],"techniques":[23],"to":[24,37,51],"yield":[25],"bivalent":[26],"inhibitors.":[27],"A":[28],"pair":[29],"fragments":[31],"is":[32,69],"mixed":[33],"and":[34,56,72],"covalently":[35],"attached":[36],"glass":[39],"slide":[40],"by":[41],"photolinking":[42],"immobilization.":[43],"The":[44],"method":[45],"does":[46],"require":[48],"compounds":[50],"have":[52],"specific":[53],"functional":[54],"groups,":[55],"tedious":[57],"steps":[58],"for":[59,74],"protein":[60],"purification":[61],"avoided.":[63],"Thus,":[64],"on\u2010chip":[66],"relatively":[70],"simple":[71],"efficient":[73],"obtaining":[75],"high\u2010affinity":[76],"ligands.":[77],"magnified":[78],"image":[79]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}