{"id":"https://openalex.org/W2092017537","doi":"https://doi.org/10.1002/bmc.208","title":"Capillary electrophoresis and enzyme solid phase assay for examining the purity of a synthetic heparin proteoglycan\u2010like conjugate and identifying binding to basic fibroblast growth factor","display_name":"Capillary electrophoresis and enzyme solid phase assay for examining the purity of a synthetic heparin proteoglycan\u2010like conjugate and identifying binding to basic fibroblast growth factor","publication_year":2003,"publication_date":"2003-01-01","ids":{"openalex":"https://openalex.org/W2092017537","doi":"https://doi.org/10.1002/bmc.208","mag":"2092017537","pmid":"https://pubmed.ncbi.nlm.nih.gov/12583005"},"language":"en","primary_location":{"id":"doi:10.1002/bmc.208","doi":"https://doi.org/10.1002/bmc.208","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bmc.208","pdf_url":null,"source":{"id":"https://openalex.org/S123979729","display_name":"Biomedical Chromatography","issn_l":"0269-3879","issn":["0269-3879","1099-0801"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedical Chromatography","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5067782722","display_name":"V. Dimitrellos","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I174878644","display_name":"University of Patras","ror":"https://ror.org/017wvtq80","country_code":"GR","type":"education","lineage":["https://openalex.org/I174878644"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"V. Dimitrellos","raw_affiliation_strings":["Department of Chemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Laboratory of Biochemistry, University of Patras, 261 10 Patras, Greece"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Laboratory of Biochemistry, University of Patras, 261 10 Patras, Greece","institution_ids":["https://openalex.org/I174878644"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041852809","display_name":"Fotini N. Lamari","orcid":"https://orcid.org/0000-0002-6938-3500","observed_orcids":["https://orcid.org/0000-0002-6938-3500"]},"institutions":[{"id":"https://openalex.org/I174878644","display_name":"University of Patras","ror":"https://ror.org/017wvtq80","country_code":"GR","type":"education","lineage":["https://openalex.org/I174878644"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"F. N. Lamari","raw_affiliation_strings":["Department of Chemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Laboratory of Biochemistry, University of Patras, 261 10 Patras, Greece"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Laboratory of Biochemistry, University of Patras, 261 10 Patras, Greece","institution_ids":["https://openalex.org/I174878644"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084098283","display_name":"Maria Militsopoulou","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I174878644","display_name":"University of Patras","ror":"https://ror.org/017wvtq80","country_code":"GR","type":"education","lineage":["https://openalex.org/I174878644"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"M. Militsopoulou","raw_affiliation_strings":["Department of Chemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Laboratory of Biochemistry, University of Patras, 261 10 Patras, Greece"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Laboratory of Biochemistry, University of Patras, 261 10 Patras, Greece","institution_ids":["https://openalex.org/I174878644"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037481344","display_name":"Ioannis Kanakis","orcid":"https://orcid.org/0000-0001-6410-1482","observed_orcids":["https://orcid.org/0000-0001-6410-1482"]},"institutions":[{"id":"https://openalex.org/I174878644","display_name":"University of Patras","ror":"https://ror.org/017wvtq80","country_code":"GR","type":"education","lineage":["https://openalex.org/I174878644"]}],"countries":["GR"],"is_corresponding":false,"raw_author_name":"I. Kanakis","raw_affiliation_strings":["Department of Chemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Laboratory of Biochemistry, University of Patras, 261 10 Patras, Greece"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Laboratory of Biochemistry, University of Patras, 261 10 Patras, Greece","institution_ids":["https://openalex.org/I174878644"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5012410418","display_name":"Nikos K. Karamanos","orcid":"https://orcid.org/0000-0003-3618-0288","observed_orcids":["https://orcid.org/0000-0003-3618-0288"]},"institutions":[{"id":"https://openalex.org/I174878644","display_name":"University of Patras","ror":"https://ror.org/017wvtq80","country_code":"GR","type":"education","lineage":["https://openalex.org/I174878644"]}],"countries":["GR"],"is_corresponding":true,"raw_author_name":"N. K. Karamanos","raw_affiliation_strings":["Department of Chemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Laboratory of Biochemistry, University of Patras, 261 10 Patras, Greece"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Laboratory of Biochemistry, University of Patras, 261 10 Patras, Greece","institution_ids":["https://openalex.org/I174878644"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5012410418"],"corresponding_institution_ids":["https://openalex.org/I174878644"],"apc_list":{"value":3710,"currency":"USD","value_usd":3710},"apc_paid":null,"fwci":0.0838,"has_fulltext":false,"cited_by_count":8,"citation_normalized_percentile":{"value":0.35746988,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":93},"biblio":{"volume":"17","issue":"1","first_page":"42","last_page":"47"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12287","display_name":"Fibroblast Growth Factor Research","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heparin","display_name":"heparin","score":0.8309999704360962},{"id":"https://openalex.org/keywords/heparan-sulfate","display_name":"heparan sulfate","score":0.9670000076293945},{"id":"https://openalex.org/keywords/basic-fibroblast-growth-factor","display_name":"basic fibroblast growth factor","score":0.9909999966621399},{"id":"https://openalex.org/keywords/growth-factor-binding","display_name":"growth factor binding","score":0.5609999895095825},{"id":"https://openalex.org/keywords/glycosaminoglycans","display_name":"glycosaminoglycans","score":0.9120000004768372},{"id":"https://openalex.org/keywords/competitive-binding-assay","display_name":"competitive binding assay","score":0.6940000057220459},{"id":"https://openalex.org/keywords/sulfated-disaccharides","display_name":"sulfated disaccharides","score":0.7820000052452087},{"id":"https://openalex.org/keywords/chondroitin-sulfate","display_name":"chondroitin sulfate","score":0.7919999957084656},{"id":"https://openalex.org/keywords/dermatan-sulfate","display_name":"dermatan sulfate","score":0.9950000047683716},{"id":"https://openalex.org/keywords/hyaluronic-acid","display_name":"hyaluronic acid","score":0.9760000109672546},{"id":"https://openalex.org/keywords/reductive-amination","display_name":"reductive amination","score":0.7200000286102295},{"id":"https://openalex.org/keywords/capillary-zone-electrophoresis","display_name":"capillary zone electrophoresis","score":0.8220000267028809}],"concepts":[{"id":"https://openalex.org/C2777557582","wikidata":"https://www.wikidata.org/wiki/Q190016","display_name":"Heparin","level":2,"score":0.9025083780288696},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8489284515380859},{"id":"https://openalex.org/C2778041096","wikidata":"https://www.wikidata.org/wiki/Q424668","display_name":"Heparan sulfate","level":3,"score":0.8359479904174805},{"id":"https://openalex.org/C2777587049","wikidata":"https://www.wikidata.org/wiki/Q20969945","display_name":"Basic fibroblast growth factor","level":4,"score":0.6805515289306641},{"id":"https://openalex.org/C153074725","wikidata":"https://www.wikidata.org/wiki/Q407553","display_name":"Glycosaminoglycan","level":2,"score":0.6795810461044312},{"id":"https://openalex.org/C2779553658","wikidata":"https://www.wikidata.org/wiki/Q408014","display_name":"Chondroitin sulfate","level":3,"score":0.6253629922866821},{"id":"https://openalex.org/C2778066728","wikidata":"https://www.wikidata.org/wiki/Q2335618","display_name":"Dermatan sulfate","level":4,"score":0.603766679763794},{"id":"https://openalex.org/C2776735749","wikidata":"https://www.wikidata.org/wiki/Q4862650","display_name":"Chondroitin","level":3,"score":0.49796462059020996},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4923795461654663},{"id":"https://openalex.org/C197336794","wikidata":"https://www.wikidata.org/wiki/Q5161150","display_name":"Conjugate","level":2,"score":0.4828246533870697},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4647044241428375},{"id":"https://openalex.org/C2779335624","wikidata":"https://www.wikidata.org/wiki/Q407976","display_name":"Proteoglycan","level":3,"score":0.46087175607681274},{"id":"https://openalex.org/C74373430","wikidata":"https://www.wikidata.org/wiki/Q24727126","display_name":"Fibroblast growth factor","level":3,"score":0.44363337755203247},{"id":"https://openalex.org/C2775960820","wikidata":"https://www.wikidata.org/wiki/Q408378","display_name":"Growth factor","level":3,"score":0.3359510004520416},{"id":"https://openalex.org/C189165786","wikidata":"https://www.wikidata.org/wiki/Q193825","display_name":"Extracellular matrix","level":2,"score":0.145513117313385},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0647452175617218},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006493","descriptor_name":"Heparin","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D006493","descriptor_name":"Heparin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011509","descriptor_name":"Proteoglycans","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D011509","descriptor_name":"Proteoglycans","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016222","descriptor_name":"Fibroblast Growth Factor 2","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D019075","descriptor_name":"Electrophoresis, Capillary","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/bmc.208","doi":"https://doi.org/10.1002/bmc.208","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bmc.208","pdf_url":null,"source":{"id":"https://openalex.org/S123979729","display_name":"Biomedical Chromatography","issn_l":"0269-3879","issn":["0269-3879","1099-0801"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedical Chromatography","raw_type":"journal-article"},{"id":"pmid:12583005","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12583005","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedical chromatography : BMC","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.5683000087738037}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321916","display_name":"State Scholarships Foundation","ror":"https://ror.org/023z3dm33"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W47249135","https://openalex.org/W69667449","https://openalex.org/W386691860","https://openalex.org/W635381471","https://openalex.org/W1494551606","https://openalex.org/W1501818109","https://openalex.org/W1578995377","https://openalex.org/W1585322583","https://openalex.org/W1595861603","https://openalex.org/W1965144118","https://openalex.org/W1972855766","https://openalex.org/W1973840148","https://openalex.org/W1974873948","https://openalex.org/W1992387854","https://openalex.org/W1996207927","https://openalex.org/W1999652145","https://openalex.org/W2035248906","https://openalex.org/W2050256171","https://openalex.org/W2095113001","https://openalex.org/W2100837269","https://openalex.org/W2108992022","https://openalex.org/W2128635872","https://openalex.org/W2165233782","https://openalex.org/W2328455531"],"related_works":["https://openalex.org/W1991222975","https://openalex.org/W2072459218","https://openalex.org/W2163170931","https://openalex.org/W7097709034","https://openalex.org/W1965479623","https://openalex.org/W1504197390","https://openalex.org/W2225212339","https://openalex.org/W2035248906","https://openalex.org/W217979527","https://openalex.org/W1487884828"],"abstract_inverted_index":{"Interaction":[0],"of":[1,29,60,79,121,138,145,154,226,258],"basic":[2],"fibroblast":[3],"growth":[4,13],"factor":[5,14],"(bFGF)":[6],"with":[7,118,135,156,224],"heparin/heparan":[8,233],"sulfate":[9,33,99,109,217],"proteoglycans":[10,48],"protects":[11],"the":[12,26,35,54,57,77,83,119,201,206],"against":[15],"proteolytic":[16],"degradation":[17],"and":[18,31,56,100,107,168,189,232,246,248],"is":[19,244],"essential":[20],"for":[21,34,113,158],"its":[22],"cellular":[23],"activity.":[24],"Although":[25],"structural":[27],"requirements":[28],"heparin":[30,47,62,106,204,256],"heparan":[32,98,108],"high-affinity":[36],"binding":[37,58,81,120,209],"to":[38,82,116,123,210,253],"bFGF":[39,80,122,157,211],"have":[40],"been":[41],"extensively":[42],"examined,":[43],"studies":[44],"on":[45],"intact":[46],"are":[49],"limited.":[50],"In":[51],"this":[52],"report,":[53],"purity":[55],"ability":[59,115],"a":[61,219],"proteoglycan-like":[63],"molecule-the":[64],"heparin-bovine":[65],"serum":[66],"albumin":[67],"(heparin-BSA)":[68],"conjugate-was":[69],"examined":[70],"using":[71,88,176],"capillary":[72],"zone":[73],"electrophoresis":[74],"(CZE).":[75],"Furthermore,":[76],"affinity":[78,208,221],"heparin-BSA":[84,155,167],"conjugate":[85,126,149],"was":[86,127,142,150],"studied":[87,112],"an":[89],"enzyme":[90],"solid-phase":[91],"assay.":[92],"Chondroitin":[93],"sulfate,":[94,96],"dermatan":[95],"hyaluronan,":[97],"variously":[101],"sulfated":[102],"disaccharides":[103,235],"derived":[104],"from":[105],"were":[110,171],"also":[111],"their":[114],"compete":[117],"heparin.":[124,147],"Heparin-BSA":[125],"synthesized":[128],"by":[129,174],"reductive":[130],"amination":[131],"and,":[132],"following":[133,152],"precipitation":[134],"1.5":[136],"vols":[137],"ethanol-sodium":[139],"acetate,":[140],"it":[141],"obtained":[143,151],"free":[144],"contaminating":[146],"Heparin-BSA-bFGF":[148],"incubation":[153],"2":[159],"h":[160],"at":[161,191],"37":[162],"degrees":[163],"C.":[164],"Intact":[165],"heparin,":[166,227],"heparin-BSA-bFGF":[169],"conjugates":[170],"completely":[172],"resolved":[173],"CZE":[175,242],"50":[177],"mM":[178],"phosphate,":[179],"pH":[180],"3.5,":[181],"as":[182,222],"operating":[183],"buffer,":[184],"reversed":[185],"polarity":[186],"(30":[187],"kV)":[188],"detection":[190],"232":[192],"nm.":[193],"Competitive":[194],"solid":[195],"phase":[196],"assay":[197],"showed":[198,218,237],"that,":[199],"among":[200],"glycosaminoglycans":[202,231],"tested,":[203],"exhibits":[205],"highest":[207],"(IC(50)":[212],"=":[213],"6.4":[214],"nM).":[215],"Heparan":[216],"lower":[220],"compared":[223],"that":[225],"whereas":[228],"all":[229],"other":[230],"sulfate-derived":[234],"tested":[236],"minute":[238],"effects.":[239],"The":[240],"developed":[241],"method":[243],"rapid":[245],"accurate":[247],"can":[249],"be":[250],"easily":[251],"used":[252],"identify":[254],"bFGF-interacting":[255],"preparations":[257],"biopharmaceutical":[259],"importance.":[260]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}