{"id":"https://openalex.org/W2092014257","doi":"https://doi.org/10.1590/s0103-84781999000100020","title":"Criopreserva\u00e7\u00e3o de embri\u00f5es de su\u00ednos adicionando trealose aos crioprotetores etilenoglicol ou glicerol","display_name":"Criopreserva\u00e7\u00e3o de embri\u00f5es de su\u00ednos adicionando trealose aos crioprotetores etilenoglicol ou glicerol","publication_year":1999,"publication_date":"1999-03-01","ids":{"openalex":"https://openalex.org/W2092014257","doi":"https://doi.org/10.1590/s0103-84781999000100020","mag":"2092014257"},"language":"pt","primary_location":{"id":"doi:10.1590/s0103-84781999000100020","doi":"https://doi.org/10.1590/s0103-84781999000100020","is_oa":true,"landing_page_url":"https://doi.org/10.1590/s0103-84781999000100020","pdf_url":"https://www.scielo.br/j/cr/a/5WwFHrpcqbf7hYwZSXNP9VH/?lang=pt&format=pdf","source":{"id":"https://openalex.org/S140190424","display_name":"Ci\u00eancia Rural","issn_l":"0103-8478","issn":["0103-8478","1678-4596"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","ki-jl-1","norway-1","poland-40","russia-white-list-3","scielo"],"host_organization":"https://openalex.org/P4310312382","host_organization_name":"Universidade Federal de Santa Maria","host_organization_lineage":["https://openalex.org/P4310312382"],"host_organization_lineage_names":["Universidade Federal de Santa Maria"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Ci\u00eancia Rural","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.scielo.br/j/cr/a/5WwFHrpcqbf7hYwZSXNP9VH/?lang=pt&format=pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057246720","display_name":"Edmir S. Nicola","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Edmir da Silva Nicola","raw_affiliation_strings":["Universidade Federal de Pelotas"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidade Federal de Pelotas","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030839962","display_name":"Jo\u00e3o Carlos Deschamps","orcid":"https://orcid.org/0009-0002-4274-8677","observed_orcids":["https://orcid.org/0009-0002-4274-8677"]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Jo\u00e3o Carlos Deschamps","raw_affiliation_strings":["Universidade Federal de Pelotas","Universidade Federal de Pelotas, PELOTAS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidade Federal de Pelotas","institution_ids":["https://openalex.org/I169248161"]},{"raw_affiliation_string":"Universidade Federal de Pelotas, PELOTAS","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019229492","display_name":"Milton C. Macedo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I11385950","display_name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","ror":"https://ror.org/03swz6y49","country_code":"BR","type":"government","lineage":["https://openalex.org/I11385950","https://openalex.org/I4210151455"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Milton Macedo J\u00fanior","raw_affiliation_strings":["Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","institution_ids":["https://openalex.org/I11385950"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5077956790","display_name":"Jos\u00e9 Bozato Sobrinho","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jos\u00e9 Bozato Sobrinho","raw_affiliation_strings":["M\u00e9dico Veterin\u00e1rio"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"M\u00e9dico Veterin\u00e1rio","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":700,"currency":"BRL","value_usd":133},"apc_paid":{"value":700,"currency":"BRL","value_usd":133},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"29","issue":"1","first_page":"111","last_page":"115"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.9057999849319458,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.9057999849319458,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.04569999873638153,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.024900000542402267,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/trehalose","display_name":"trehalose","score":0.9919999837875366},{"id":"https://openalex.org/keywords/ethylene-glycol","display_name":"ethylene glycol","score":0.8949999809265137},{"id":"https://openalex.org/keywords/glycerol","display_name":"glycerol","score":0.996999979019165},{"id":"https://openalex.org/keywords/cryoprotectants","display_name":"cryoprotectants","score":0.925000011920929},{"id":"https://openalex.org/keywords/embryo-viability","display_name":"embryo viability","score":0.6320000290870667},{"id":"https://openalex.org/keywords/blastocyst","display_name":"blastocyst","score":0.6729999780654907},{"id":"https://openalex.org/keywords/quick-freezing","display_name":"rapid freezing","score":0.6299999952316284},{"id":"https://openalex.org/keywords/slow-freezing","display_name":"slow freezing","score":0.515999972820282},{"id":"https://openalex.org/keywords/thawing","display_name":"thawing","score":0.550000011920929},{"id":"https://openalex.org/keywords/expanded-blastocysts","display_name":"expanded blastocysts","score":0.7059999704360962},{"id":"https://openalex.org/keywords/embryo-expansion","display_name":"embryo expansion","score":0.33000001311302185},{"id":"https://openalex.org/keywords/embryo-culture","display_name":"embryo culture","score":0.5339999794960022}],"concepts":[{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.3618658483028412},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3277311325073242}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.1590/s0103-84781999000100020","doi":"https://doi.org/10.1590/s0103-84781999000100020","is_oa":true,"landing_page_url":"https://doi.org/10.1590/s0103-84781999000100020","pdf_url":"https://www.scielo.br/j/cr/a/5WwFHrpcqbf7hYwZSXNP9VH/?lang=pt&format=pdf","source":{"id":"https://openalex.org/S140190424","display_name":"Ci\u00eancia Rural","issn_l":"0103-8478","issn":["0103-8478","1678-4596"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","ki-jl-1","norway-1","poland-40","russia-white-list-3","scielo"],"host_organization":"https://openalex.org/P4310312382","host_organization_name":"Universidade Federal de Santa Maria","host_organization_lineage":["https://openalex.org/P4310312382"],"host_organization_lineage_names":["Universidade Federal de Santa Maria"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Ci\u00eancia Rural","raw_type":"journal-article"},{"id":"pmh:oai:scielo:S0103-84781999000100020","doi":null,"is_oa":true,"landing_page_url":"http://old.scielo.br/scielo.php?script=sci_arttext&amp;pid=S0103-84781999000100020","pdf_url":null,"source":{"id":"https://openalex.org/S4306402641","display_name":"LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Cient\u00edficas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4383465926","host_organization_name":"LA Referencia","host_organization_lineage":["https://openalex.org/I4383465926"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Ci\u00eancia Rural v.29 n.1 1999","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:americanae.aecid.es:2385903","doi":null,"is_oa":true,"landing_page_url":"http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000100020","pdf_url":null,"source":{"id":"https://openalex.org/S4306400786","display_name":"Americanae (AECID Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Ci\u00eancia Rural v.29 n.1 1999","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:doaj.org/article:98fefcacc57048b8a6bf5e353cdebdc5","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/98fefcacc57048b8a6bf5e353cdebdc5","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Ci\u00eancia Rural, Vol 29, Iss 1, Pp 111-115 (1999)","raw_type":"article"},{"id":"mag:2742115188","doi":null,"is_oa":false,"landing_page_url":"http://www.scielo.br/pdf/cr/v29n1/a20v29n1.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"doi:10.1590/s0103-84781999000100020","doi":"https://doi.org/10.1590/s0103-84781999000100020","is_oa":true,"landing_page_url":"https://doi.org/10.1590/s0103-84781999000100020","pdf_url":"https://www.scielo.br/j/cr/a/5WwFHrpcqbf7hYwZSXNP9VH/?lang=pt&format=pdf","source":{"id":"https://openalex.org/S140190424","display_name":"Ci\u00eancia Rural","issn_l":"0103-8478","issn":["0103-8478","1678-4596"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","ki-jl-1","norway-1","poland-40","russia-white-list-3","scielo"],"host_organization":"https://openalex.org/P4310312382","host_organization_name":"Universidade Federal de Santa Maria","host_organization_lineage":["https://openalex.org/P4310312382"],"host_organization_lineage_names":["Universidade Federal de Santa Maria"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Ci\u00eancia Rural","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Zero hunger","score":0.875,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2092014257.pdf","grobid_xml":"https://content.openalex.org/works/W2092014257.grobid-xml"},"referenced_works_count":24,"referenced_works":["https://openalex.org/W1545462020","https://openalex.org/W1833593555","https://openalex.org/W1967284317","https://openalex.org/W1987077558","https://openalex.org/W1994882048","https://openalex.org/W2005835660","https://openalex.org/W2014833154","https://openalex.org/W2017009051","https://openalex.org/W2019534716","https://openalex.org/W2023811614","https://openalex.org/W2030060567","https://openalex.org/W2040272213","https://openalex.org/W2042405141","https://openalex.org/W2052412410","https://openalex.org/W2067131371","https://openalex.org/W2077206617","https://openalex.org/W2078898589","https://openalex.org/W2082086213","https://openalex.org/W2131029585","https://openalex.org/W2139381506","https://openalex.org/W2141757772","https://openalex.org/W2312485606","https://openalex.org/W2397338032","https://openalex.org/W2411133337"],"related_works":["https://openalex.org/W2742115188","https://openalex.org/W2788542087","https://openalex.org/W7043996707","https://openalex.org/W2565887198","https://openalex.org/W4254283691","https://openalex.org/W7056307114","https://openalex.org/W7120542331","https://openalex.org/W50394751","https://openalex.org/W7120317476","https://openalex.org/W7044182919"],"abstract_inverted_index":{"Os":[0,77,293],"objetivos":[1],"deste":[2],"trabalho":[3],"foram:":[4],"comparar":[5],"(a)":[6],"o":[7,18,92,116,252,270,308],"efeito":[8,19],"da":[9,196,198],"adi\u00e7\u00e3o":[10,323],"de":[11,31,34,43,45,51,64,71,73,83,88,110,113,122,124,132,137,139,144,190,205,209,223,225,231,243,256,272,274,280,316,324,328,333],"trealose":[12,27,38,325],"ao":[13,164,305],"crioprotetor":[14],"etilenoglicol":[15,22,46,347],"e,":[16,169],"(b)":[17],"dos":[20,180,201],"crioprotetores":[21,181,346],"ou":[23,348],"glicerol":[24,74],"associados":[25,350],"\u00e0":[26,41,69,127,173,331,351],"sobre":[28],"a":[29,97,103,108,130,135,142,276,314],"viabilidade":[30,191,248,277],"embri\u00f5es":[32,78,317],"congelados":[33,80],"su\u00ednos":[35],".":[36],"A":[37,146,178,247],"foi":[39,91,112,118,158,182,213,255,279,319,340],"adicionada":[40],"solu\u00e7\u00e3o":[42,70,332],"congelamento":[44,72,89],"1.5":[47,75],"M":[48,53,58,66,330],"nas":[49],"concentra\u00e7\u00f5es":[50],"0.0":[52],"(tratamento":[54,59,67],"1)":[55],"e":[56,61,245,259,266,283,290],"0.25":[57,65,329],"2),":[60],"na":[62,194,326],"concentra\u00e7\u00e3o":[63,327],"3)":[68],"M.":[76],"foram":[79,150],"no":[81,152],"est\u00e1gio":[82],"blastocisto":[84],"expandido.":[85],"O":[86,156,188,211],"m\u00e9todo":[87,185],"utilizado":[90],"r\u00e1pido,":[93],"sendo":[94],"que":[95,296,313],"desde":[96],"temperatura":[98,104,143],"ambiente":[99],"(\u00b1":[100],"25\u00b0C)":[101],"at\u00e9":[102,141],"do":[105],"\"seeding\"":[106],"(-7\u00b0C),":[107],"velocidade":[109,136],"resfriamento":[111,138],"1\u00b0C/minuto.":[114],"Ap\u00f3s":[115,269,307],"\"seeding\",":[117],"estabelecido":[119],"um":[120],"per\u00edodo":[121,204,271],"equil\u00edbrio":[123],"10":[125],"minutos":[126],"-7\u00b0C,":[128],"sendo,":[129],"partir":[131],"ent\u00e3o,":[133],"adotada":[134],"0.3\u00b0C/minuto":[140],"-35\u00b0C.":[145],"seguir,":[147],"as":[148],"palhetas":[149,163],"colocadas":[151],"nitrog\u00eanio":[153],"l\u00edquido":[154],"(-196\u00b0C).":[155],"descongelamento":[157,253],"realizado":[159],"mediante":[160],"exposi\u00e7\u00e3o":[161],"das":[162],"ar":[165],"durante":[166,175],"30":[167,176],"segundos":[168],"posteriormente,":[170],"em":[171,186,215,229,237],"banho-maria":[172],"35\u00b0C,":[174],"segundos.":[177],"remo\u00e7\u00e3o":[179],"realizada":[183],"pelo":[184],"etapas.":[187],"crit\u00e9rio":[189],"adotado":[192],"consistiu":[193],"observa\u00e7\u00e3o":[195],"reexpans\u00e3o":[197],"cavidade":[199],"blastoc\u00e9lica":[200],"embri\u00f5es,":[202],"ap\u00f3s":[203,251],"cultivo":[206,212,273,309],"in":[207,310],"vitro":[208],"18-24h.":[210],"feito":[214],"meio":[216],"TCM":[217],"199":[218],"(tissue":[219],"culture":[220],"medium),":[221],"acrescido":[222],"20%":[224],"soro":[226],"fetal":[227],"bovino,":[228],"microgotas":[230],"60":[232],"mil,":[233],"sob":[234],"\u00f3leo":[235],"mineral":[236],"estufa":[238],"com":[239],"6%":[240],"CO2,":[241],"95%":[242],"umidade":[244],"37\u00b0C.":[246],"embrion\u00e1ria":[249,278],"logo":[250],"(0h)":[254],"17.2%,":[257],"37.5%":[258],"42.8%,":[260],"para":[261,285],"os":[262,286,303,345],"tratamentos":[263,287,304],"1,":[264,288],"2":[265,289],"3,":[267,291],"respectivamente.":[268,292],"18-24h,":[275],"6.9%,":[281],"28.1%":[282],"28.5%,":[284],"resultados":[294],"indicaram":[295],"n\u00e3o":[297,339],"houve":[298],"diferen\u00e7a":[299,342],"(p>0.05)":[300],"significativa":[301,343],"entre":[302,344],"descongelamento.":[306],"vitro,":[311],"verificou-se":[312],"porcentagem":[315],"vi\u00e1veis":[318],"superior":[320],"(p<0.05)":[321],"pela":[322],"congelamento,":[334],"contendo":[335],"etilenoglicol.":[336],"No":[337],"entanto,":[338],"detectada":[341],"glicerol,":[349],"trealose.":[352]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}