{"id":"https://openalex.org/W2092010874","doi":"https://doi.org/10.1002/bdra.20654","title":"Elevated glucose induces congenital heart defects by altering the expression of tbx5, tbx20, and has2 in developing zebrafish embryos","display_name":"Elevated glucose induces congenital heart defects by altering the expression of tbx5, tbx20, and has2 in developing zebrafish embryos","publication_year":2010,"publication_date":"2010-03-19","ids":{"openalex":"https://openalex.org/W2092010874","doi":"https://doi.org/10.1002/bdra.20654","mag":"2092010874","pmid":"https://pubmed.ncbi.nlm.nih.gov/20306498"},"language":"en","primary_location":{"id":"doi:10.1002/bdra.20654","doi":"https://doi.org/10.1002/bdra.20654","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bdra.20654","pdf_url":null,"source":{"id":"https://openalex.org/S4394736882","display_name":"Birth Defects Research Part A Clinical and Molecular Teratology","issn_l":"1542-0752","issn":["1542-0752","1542-0760"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["jufo-1","poland-70"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Birth Defects Research Part A: Clinical and Molecular Teratology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061280234","display_name":"Jin-tao Liang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24943067","display_name":"Fudan University","ror":"https://ror.org/013q1eq08","country_code":"CN","type":"education","lineage":["https://openalex.org/I24943067"]},{"id":"https://openalex.org/I4210159329","display_name":"Children's Hospital of Fudan University","ror":"https://ror.org/05n13be63","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210159329"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jintao Liang","raw_affiliation_strings":["Children's Hospital, Fudan University, Shanghai, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Children's Hospital, Fudan University, Shanghai, PR China","institution_ids":["https://openalex.org/I24943067","https://openalex.org/I4210159329"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102008644","display_name":"Yonghao Gui","orcid":"https://orcid.org/0000-0002-4302-8375","observed_orcids":["https://orcid.org/0000-0002-4302-8375"]},"institutions":[{"id":"https://openalex.org/I24943067","display_name":"Fudan University","ror":"https://ror.org/013q1eq08","country_code":"CN","type":"education","lineage":["https://openalex.org/I24943067"]},{"id":"https://openalex.org/I4210159329","display_name":"Children's Hospital of Fudan University","ror":"https://ror.org/05n13be63","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210159329"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Yonghao Gui","raw_affiliation_strings":["Children's Hospital, Fudan University, Shanghai, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Children's Hospital, Fudan University, Shanghai, PR China","institution_ids":["https://openalex.org/I24943067","https://openalex.org/I4210159329"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100369593","display_name":"Wei Wang","orcid":"https://orcid.org/0000-0002-1356-7247","observed_orcids":["https://orcid.org/0000-0002-1356-7247"]},"institutions":[{"id":"https://openalex.org/I24943067","display_name":"Fudan University","ror":"https://ror.org/013q1eq08","country_code":"CN","type":"education","lineage":["https://openalex.org/I24943067"]},{"id":"https://openalex.org/I4210159329","display_name":"Children's Hospital of Fudan University","ror":"https://ror.org/05n13be63","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210159329"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wei Wang","raw_affiliation_strings":["Children's Hospital, Fudan University, Shanghai, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Children's Hospital, Fudan University, Shanghai, PR China","institution_ids":["https://openalex.org/I24943067","https://openalex.org/I4210159329"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029760703","display_name":"Shan Gao","orcid":"https://orcid.org/0000-0003-4832-5739","observed_orcids":["https://orcid.org/0000-0003-4832-5739"]},"institutions":[{"id":"https://openalex.org/I24943067","display_name":"Fudan University","ror":"https://ror.org/013q1eq08","country_code":"CN","type":"education","lineage":["https://openalex.org/I24943067"]},{"id":"https://openalex.org/I4210159329","display_name":"Children's Hospital of Fudan University","ror":"https://ror.org/05n13be63","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210159329"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shan Gao","raw_affiliation_strings":["Children's Hospital, Fudan University, Shanghai, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Children's Hospital, Fudan University, Shanghai, PR China","institution_ids":["https://openalex.org/I24943067","https://openalex.org/I4210159329"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Jing Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24943067","display_name":"Fudan University","ror":"https://ror.org/013q1eq08","country_code":"CN","type":"education","lineage":["https://openalex.org/I24943067"]},{"id":"https://openalex.org/I4210159329","display_name":"Children's Hospital of Fudan University","ror":"https://ror.org/05n13be63","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210159329"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jing Li","raw_affiliation_strings":["Children's Hospital, Fudan University, Shanghai, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Children's Hospital, Fudan University, Shanghai, PR China","institution_ids":["https://openalex.org/I24943067","https://openalex.org/I4210159329"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100827325","display_name":"Houyan Song","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I133868755","display_name":"Shanghai Medical College of Fudan University","ror":"https://ror.org/01zntxs11","country_code":"CN","type":"education","lineage":["https://openalex.org/I133868755","https://openalex.org/I24943067"]},{"id":"https://openalex.org/I24943067","display_name":"Fudan University","ror":"https://ror.org/013q1eq08","country_code":"CN","type":"education","lineage":["https://openalex.org/I24943067"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Houyan Song","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, Shanghai Medical School and Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, Shanghai, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, Shanghai Medical School and Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, Shanghai, PR China","institution_ids":["https://openalex.org/I133868755","https://openalex.org/I24943067"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5102008644"],"corresponding_institution_ids":["https://openalex.org/I24943067","https://openalex.org/I4210159329"],"apc_list":null,"apc_paid":null,"fwci":0.7432,"has_fulltext":false,"cited_by_count":30,"citation_normalized_percentile":{"value":0.64309689,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"88","issue":"6","first_page":"480","last_page":"486"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11677","display_name":"Congenital heart defects research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11677","display_name":"Congenital heart defects research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11685","display_name":"Zebrafish Biomedical Research Applications","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/congenital-heart-disease","display_name":"congenital heart disease","score":0.9200000166893005},{"id":"https://openalex.org/keywords/hyperglycemia","display_name":"hyperglycemia","score":0.9800000190734863},{"id":"https://openalex.org/keywords/maternal-diabetes","display_name":"maternal diabetes","score":0.8889999985694885},{"id":"https://openalex.org/keywords/teratogenicity","display_name":"teratogenicity","score":0.6349999904632568},{"id":"https://openalex.org/keywords/cardiac-looping","display_name":"heart looping","score":0.8600000143051147},{"id":"https://openalex.org/keywords/tbx5","display_name":"TBX5","score":0.8539999723434448},{"id":"https://openalex.org/keywords/tbx20","display_name":"Tbx20","score":1.0},{"id":"https://openalex.org/keywords/hyaluronan-synthase-2","display_name":"HAS2","score":0.9900000095367432},{"id":"https://openalex.org/keywords/d-glucose","display_name":"D-glucose","score":0.6890000104904175},{"id":"https://openalex.org/keywords/l-glucose","display_name":"L-glucose","score":0.9700000286102295},{"id":"https://openalex.org/keywords/zebrafish-embryos","display_name":"zebrafish embryos","score":0.8109999895095825},{"id":"https://openalex.org/keywords/endocardial-cushion","display_name":"endocardial cushion","score":0.8220000267028809},{"id":"https://openalex.org/keywords/diabetes-in-pregnancy","display_name":"diabetes in pregnancy","score":0.6470000147819519}],"concepts":[{"id":"https://openalex.org/C62425271","wikidata":"https://www.wikidata.org/wiki/Q14819288","display_name":"Heart development","level":4,"score":0.863805890083313},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.7781074047088623},{"id":"https://openalex.org/C2776878037","wikidata":"https://www.wikidata.org/wiki/Q169444","display_name":"Zebrafish","level":3,"score":0.7628896236419678},{"id":"https://openalex.org/C30503417","wikidata":"https://www.wikidata.org/wiki/Q958938","display_name":"Organogenesis","level":3,"score":0.6732518672943115},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5701904296875},{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.561514675617218},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.4813859760761261},{"id":"https://openalex.org/C40692019","wikidata":"https://www.wikidata.org/wiki/Q815547","display_name":"Morphogenesis","level":3,"score":0.41732436418533325},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.4131254255771637},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.39834290742874146},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.39243075251579285},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.29579079151153564},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.26418405771255493},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2309553325176239},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.10855314135551453},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.09680840373039246},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09181678295135498}],"mesh":[{"descriptor_ui":"D000076002","descriptor_name":"Hyaluronan Synthases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D006330","descriptor_name":"Heart Defects, Congenital","qualifier_ui":"Q000209","qualifier_name":"etiology","is_major_topic":true},{"descriptor_ui":"D014453","descriptor_name":"Glucuronosyltransferase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015027","descriptor_name":"Zebrafish","qualifier_ui":"Q000196","qualifier_name":"embryology","is_major_topic":true},{"descriptor_ui":"D017403","descriptor_name":"In Situ Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020825","descriptor_name":"T-Box Domain Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D029961","descriptor_name":"Zebrafish Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1002/bdra.20654","doi":"https://doi.org/10.1002/bdra.20654","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bdra.20654","pdf_url":null,"source":{"id":"https://openalex.org/S4394736882","display_name":"Birth Defects Research Part A Clinical and Molecular Teratology","issn_l":"1542-0752","issn":["1542-0752","1542-0760"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["jufo-1","poland-70"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Birth Defects Research Part A: Clinical and Molecular Teratology","raw_type":"journal-article"},{"id":"pmid:20306498","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20306498","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Birth defects research. Part A, Clinical and molecular teratology","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.983.9382","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.983.9382","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://or.nsfc.gov.cn/bitstream/00001903-5/122757/1/1000001783656.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.98580002784729,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W1495901582","https://openalex.org/W1506409704","https://openalex.org/W1511616125","https://openalex.org/W1768145300","https://openalex.org/W1969743070","https://openalex.org/W1973431915","https://openalex.org/W1975873929","https://openalex.org/W1976773752","https://openalex.org/W1980307954","https://openalex.org/W2001441849","https://openalex.org/W2002907359","https://openalex.org/W2016499666","https://openalex.org/W2032049429","https://openalex.org/W2037241011","https://openalex.org/W2065011422","https://openalex.org/W2066092773","https://openalex.org/W2069865606","https://openalex.org/W2078360903","https://openalex.org/W2084868598","https://openalex.org/W2097348463","https://openalex.org/W2101735975","https://openalex.org/W2102103202","https://openalex.org/W2102237554","https://openalex.org/W2103968215","https://openalex.org/W2107445027","https://openalex.org/W2120972584","https://openalex.org/W2122935465","https://openalex.org/W2124752518","https://openalex.org/W2125349610","https://openalex.org/W2126878764","https://openalex.org/W2128466784","https://openalex.org/W2136130289","https://openalex.org/W2147301551","https://openalex.org/W2156061910","https://openalex.org/W2158573773","https://openalex.org/W2158900245","https://openalex.org/W2158990308","https://openalex.org/W2161864778","https://openalex.org/W2617245041"],"related_works":["https://openalex.org/W4294921122","https://openalex.org/W4254402087","https://openalex.org/W4234868778","https://openalex.org/W2114374618","https://openalex.org/W3095600987","https://openalex.org/W2791015512","https://openalex.org/W640506036","https://openalex.org/W3035516376","https://openalex.org/W2059613083","https://openalex.org/W2774527609"],"abstract_inverted_index":{"BACKGROUND:":[0],"Maternal":[1],"diabetes":[2],"increases":[3],"the":[4,41,71,79,149,162,174,185,204,213,218,244],"risk":[5],"of":[6,21,30,44,100,113,148,151,164,177,207,246],"congenital":[7,247],"heart":[8,48,76,152,168,228,248],"defects":[9,153,198,249],"in":[10,57,106,184,199,212,250],"infants,":[11],"and":[12,28,133,167,180,210,238],"hyperglycemia":[13,45],"acts":[14],"as":[15],"a":[16],"major":[17],"teratogen.":[18],"Multiple":[19],"steps":[20],"cardiac":[22,114,135,156,165,197,222],"development,":[23],"including":[24],"endocardial":[25],"cushion":[26],"morphogenesis":[27],"development":[29],"neural":[31],"crest":[32],"cells,":[33],"are":[34,233],"challenged":[35],"under":[36],"elevated":[37,193],"glucose":[38,194],"conditions.":[39],"However,":[40],"direct":[42],"effect":[43],"on":[46],"embryo":[47],"organogenesis":[49],"remains":[50],"to":[51,62,69,140],"be":[52],"investigated.":[53],"METHODS:":[54],"Zebrafish":[55],"embryos":[56,85,128,138,201],"different":[58],"stages":[59],"were":[60,86,171,182],"exposed":[61,139],"D-glucose":[63,92],"for":[64,95,235],"12":[65],"or":[66,93],"24":[67,96,124],"hr":[68,83,125],"determine":[70],"sensitive":[72],"window":[73],"during":[74,118,227],"early":[75],"development.":[77],"In":[78],"subsequent":[80],"study,":[81],"6":[82],"post-fertilization":[84],"treated":[87],"with":[88],"either":[89],"25":[90],"mmol/liter":[91],"L-glucose":[94,141],"hr.":[97],"The":[98,110],"expression":[99,175,205],"genes":[101],"was":[102,116,158],"analyzed":[103],"by":[104,202],"whole-mount":[105],"situ":[107],"hybridization.":[108],"RESULTS:":[109],"highest":[111],"incidence":[112],"malformations":[115],"found":[117],"6-30":[119],"hpf":[120],"exposure":[121],"periods.":[122],"After":[123],"exposure,":[126],"D-glucose-treated":[127],"exhibited":[129],"significant":[130],"developmental":[131],"delay":[132],"diverse":[134],"malformations,":[136],"but":[137],"showed":[142,154],"no":[143],"apparent":[144],"phenotype.":[145],"Further":[146],"investigation":[147],"origin":[150],"that":[155,192,221],"looping":[157,223],"affected":[159,225],"earliest,":[160],"while":[161],"specification":[163],"progenitors":[166],"tube":[169],"assembly":[170],"complete.":[172],"Moreover,":[173],"patterns":[176],"tbx5,":[178,208],"tbx20,":[179,209],"has2":[181,211],"altered":[183],"defective":[186],"hearts.":[187],"CONCLUSIONS:":[188],"Our":[189],"data":[190],"demonstrate":[191],"alone":[195],"induces":[196],"zebrafish":[200],"altering":[203],"pattern":[206],"heart.":[214],"We":[215],"also":[216],"show":[217],"first":[219],"evidence":[220],"is":[224],"earliest":[226],"organogenesis.":[229],"These":[230],"research":[231],"results":[232],"important":[234],"devising":[236],"preventive":[237],"therapeutic":[239],"strategies":[240],"aimed":[241],"at":[242],"reducing":[243],"occurrence":[245],"diabetic":[251],"pregnancy.":[252]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":5}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}