{"id":"https://openalex.org/W2091303133","doi":"https://doi.org/10.1073/pnas.250472297","title":"Interaction of two arginine residues in lactate oxidase with the enzyme flavin: Conversion of FMN to 8-formyl-FMN","display_name":"Interaction of two arginine residues in lactate oxidase with the enzyme flavin: Conversion of FMN to 8-formyl-FMN","publication_year":2000,"publication_date":"2000-11-14","ids":{"openalex":"https://openalex.org/W2091303133","doi":"https://doi.org/10.1073/pnas.250472297","mag":"2091303133","pmid":"https://pubmed.ncbi.nlm.nih.gov/11078532","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/27174"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.250472297","doi":"https://doi.org/10.1073/pnas.250472297","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.250472297","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/27174","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5020439203","display_name":"Kazuko Yorita","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP","US"],"is_corresponding":false,"raw_author_name":"Kazuko Yorita","raw_affiliation_strings":["Institute for Enzyme Research, University of Tokushima,\r Kuramoto-cho 3-18-15, Tokushima 770-8503, Japan;\r Diagnostics Research and Development Department, Asahi\r Chemical Industry, Shizuoka 410-2321, Japan; and\r Department of Biological Chemistry, University of\r Michigan Medical School, Ann Arbor, MI 48109-0606"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Enzyme Research, University of Tokushima,\r Kuramoto-cho 3-18-15, Tokushima 770-8503, Japan;\r Diagnostics Research and Development Department, Asahi\r Chemical Industry, Shizuoka 410-2321, Japan; and\r Department of Biological Chemistry, University of\r Michigan Medical School, Ann Arbor, MI 48109-0606","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102997157","display_name":"Takeshi Matsuoka","orcid":"https://orcid.org/0000-0003-0572-8300","observed_orcids":["https://orcid.org/0000-0003-0572-8300"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP","US"],"is_corresponding":false,"raw_author_name":"Takeshi Matsuoka","raw_affiliation_strings":["Institute for Enzyme Research, University of Tokushima,\r Kuramoto-cho 3-18-15, Tokushima 770-8503, Japan;\r Diagnostics Research and Development Department, Asahi\r Chemical Industry, Shizuoka 410-2321, Japan; and\r Department of Biological Chemistry, University of\r Michigan Medical School, Ann Arbor, MI 48109-0606"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Enzyme Research, University of Tokushima,\r Kuramoto-cho 3-18-15, Tokushima 770-8503, Japan;\r Diagnostics Research and Development Department, Asahi\r Chemical Industry, Shizuoka 410-2321, Japan; and\r Department of Biological Chemistry, University of\r Michigan Medical School, Ann Arbor, MI 48109-0606","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090431165","display_name":"Hideo Misaki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP","US"],"is_corresponding":false,"raw_author_name":"Hideo Misaki","raw_affiliation_strings":["Institute for Enzyme Research, University of Tokushima,\r Kuramoto-cho 3-18-15, Tokushima 770-8503, Japan;\r Diagnostics Research and Development Department, Asahi\r Chemical Industry, Shizuoka 410-2321, Japan; and\r Department of Biological Chemistry, University of\r Michigan Medical School, Ann Arbor, MI 48109-0606"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Enzyme Research, University of Tokushima,\r Kuramoto-cho 3-18-15, Tokushima 770-8503, Japan;\r Diagnostics Research and Development Department, Asahi\r Chemical Industry, Shizuoka 410-2321, Japan; and\r Department of Biological Chemistry, University of\r Michigan Medical School, Ann Arbor, MI 48109-0606","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I922474255"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5079221179","display_name":"Vincent Massey","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP","US"],"is_corresponding":true,"raw_author_name":"Vincent Massey","raw_affiliation_strings":["Institute for Enzyme Research, University of Tokushima,\r Kuramoto-cho 3-18-15, Tokushima 770-8503, Japan;\r Diagnostics Research and Development Department, Asahi\r Chemical Industry, Shizuoka 410-2321, Japan; and\r Department of Biological Chemistry, University of\r Michigan Medical School, Ann Arbor, MI 48109-0606"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Enzyme Research, University of Tokushima,\r Kuramoto-cho 3-18-15, Tokushima 770-8503, Japan;\r Diagnostics Research and Development Department, Asahi\r Chemical Industry, Shizuoka 410-2321, Japan; and\r Department of Biological Chemistry, University of\r Michigan Medical School, Ann Arbor, MI 48109-0606","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I922474255"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5079221179"],"corresponding_institution_ids":["https://openalex.org/I27837315","https://openalex.org/I922474255"],"apc_list":null,"apc_paid":null,"fwci":1.1277,"has_fulltext":false,"cited_by_count":43,"citation_normalized_percentile":{"value":0.7800304,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"97","issue":"24","first_page":"13039","last_page":"13044"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.9253000020980835,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.9253000020980835,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Chemical Reaction Mechanisms","score":0.034699998795986176,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.00839999970048666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lactate-oxidase","display_name":"lactate oxidase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/aerococcus-viridans","display_name":"Aerococcus viridans","score":0.9010000228881836},{"id":"https://openalex.org/keywords/flavin-mononucleotide","display_name":"flavin mononucleotide","score":0.7289999723434448},{"id":"https://openalex.org/keywords/arginine-residues","display_name":"arginine residues","score":0.8119999766349792},{"id":"https://openalex.org/keywords/flavin-binding","display_name":"flavin binding","score":0.49900001287460327},{"id":"https://openalex.org/keywords/l-lactate","display_name":"L-lactate","score":0.6389999985694885},{"id":"https://openalex.org/keywords/substrate-binding","display_name":"substrate binding","score":0.4790000021457672},{"id":"https://openalex.org/keywords/charge-neutralization","display_name":"charge neutralization","score":0.8450000286102295},{"id":"https://openalex.org/keywords/transition-state","display_name":"transition state","score":0.5809999704360962},{"id":"https://openalex.org/keywords/enzyme-mutants","display_name":"enzyme mutants","score":0.4390000104904175}],"concepts":[{"id":"https://openalex.org/C33093398","wikidata":"https://www.wikidata.org/wiki/Q174337","display_name":"Flavin group","level":3,"score":0.9000197649002075},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6999309062957764},{"id":"https://openalex.org/C2776016237","wikidata":"https://www.wikidata.org/wiki/Q178430","display_name":"Lysine","level":3,"score":0.6307175755500793},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.5847070813179016},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.5343904495239258},{"id":"https://openalex.org/C2777468819","wikidata":"https://www.wikidata.org/wiki/Q173670","display_name":"Arginine","level":3,"score":0.4848731458187103},{"id":"https://openalex.org/C2779268744","wikidata":"https://www.wikidata.org/wiki/Q407479","display_name":"Oxidoreductase","level":3,"score":0.4524945914745331},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4477533996105194},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.42808935046195984},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.4270396828651428},{"id":"https://openalex.org/C75473681","wikidata":"https://www.wikidata.org/wiki/Q188651","display_name":"Photochemistry","level":1,"score":0.4028290808200836},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.15834340453147888},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.11138361692428589},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001120","descriptor_name":"Arginine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005420","descriptor_name":"Flavoproteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005420","descriptor_name":"Flavoproteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D005486","descriptor_name":"Flavin Mononucleotide","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D005486","descriptor_name":"Flavin Mononucleotide","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006899","descriptor_name":"Mixed Function Oxygenases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006899","descriptor_name":"Mixed Function Oxygenases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013289","descriptor_name":"Streptococcaceae","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017124","descriptor_name":"Conserved Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019943","descriptor_name":"Amino Acid Substitution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1073/pnas.250472297","doi":"https://doi.org/10.1073/pnas.250472297","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.250472297","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:27174","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/27174","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmid:11078532","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11078532","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Comparative Study"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:27174","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/27174","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"display_name":"Clean water and sanitation","id":"https://metadata.un.org/sdg/6","score":0.699999988079071}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2869093499","display_name":null,"funder_award_id":"GM11106","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G8591011107","display_name":"Biological Oxidation Mechanisms","funder_award_id":"R01GM011106","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332736","display_name":"Novartis Pharma","ror":"https://ror.org/0013shd50"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":26,"referenced_works":["https://openalex.org/W995099542","https://openalex.org/W1495361419","https://openalex.org/W1502554469","https://openalex.org/W1525660030","https://openalex.org/W1535516014","https://openalex.org/W1549887344","https://openalex.org/W1573201350","https://openalex.org/W1987160159","https://openalex.org/W2019119244","https://openalex.org/W2022491416","https://openalex.org/W2032585422","https://openalex.org/W2045646604","https://openalex.org/W2047131411","https://openalex.org/W2054943036","https://openalex.org/W2058960367","https://openalex.org/W2061258038","https://openalex.org/W2065314019","https://openalex.org/W2093397742","https://openalex.org/W2096312467","https://openalex.org/W2108303701","https://openalex.org/W2166094470","https://openalex.org/W2170520204","https://openalex.org/W2888511473","https://openalex.org/W3111785144","https://openalex.org/W4285826988","https://openalex.org/W6817348959"],"related_works":["https://openalex.org/W1967641998","https://openalex.org/W2093397742","https://openalex.org/W2058960367","https://openalex.org/W2054943036","https://openalex.org/W2136383407","https://openalex.org/W1502554469","https://openalex.org/W1974201161","https://openalex.org/W1992469766","https://openalex.org/W2107767348","https://openalex.org/W2076807807"],"abstract_inverted_index":{"Two":[0],"arginine":[1,127],"residues,":[2],"Arg-181":[3,47],"and":[4,39,73,134,140,190,195],"Arg-268,":[5],"are":[6,76,83,141],"conserved":[7],"throughout":[8],"the":[9,17,22,55,59,63,71,88,91,97,100,105,115,122,125,130,138,157,176,188,201],"known":[10],"family":[11],"of":[12,19,57,67,90,102,104,124,132,153,160,165,175,179,200],"FMN-containing":[13],"enzymes":[14],"that":[15,147],"catalyze":[16],"oxidation":[18],"alpha-hydroxyacids.":[20],"In":[21,45],"lactate":[23],"oxidase":[24],"from":[25,198],"Aerococcus":[26],"viridans,":[27],"these":[28,68],"residues":[29,128],"have":[30],"been":[31,49],"changed":[32],"to":[33,53,181,187],"lysine":[34,168],"in":[35,40,129,156,171],"two":[36,126],"single":[37],"mutations":[38],"a":[41,144,151,172],"double":[42],"mutant":[43,80,117],"form.":[44],"addition,":[46],"has":[48],"replaced":[50],"by":[51,108,167],"methionine":[52],"determine":[54],"effect":[56],"removing":[58],"positive":[60],"charge":[61,154],"on":[62,70,87],"residue.":[64],"The":[65,119,163],"effects":[66,86],"replacements":[69],"kinetic":[72],"thermodynamic":[74],"properties":[75,197],"reported.":[77],"With":[78],"all":[79],"forms,":[81],"there":[82],"only":[84],"small":[85],"reactivity":[89],"reduced":[92],"flavin":[93,107],"with":[94,114,137,143,191],"oxygen.":[95],"On":[96],"other":[98],"hand,":[99],"efficiency":[101],"reduction":[103],"oxidized":[106],"l-lactate":[109],"is":[110],"greatly":[111],"reduced,":[112],"particularly":[113],"R268K":[116],"forms.":[118],"results":[120,170],"demonstrate":[121],"importance":[123],"binding":[131],"substrate":[133,161],"its":[135],"interaction":[136],"flavin,":[139],"consistent":[142],"previous":[145],"hypothesis":[146],"they":[148],"also":[149,169],"play":[150],"role":[152],"neutralization":[155],"transition":[158],"state":[159],"dehydrogenation.":[162],"replacement":[164],"Arg-268":[166],"slow":[173],"conversion":[174],"8-CH(3)-":[177],"substituent":[178],"FMN":[180],"yield":[182],"8-formyl-FMN,":[183],"still":[184],"tightly":[185],"bound":[186],"enzyme,":[189],"significantly":[192],"different":[193],"physical":[194],"chemical":[196],"those":[199],"FMN-enzyme.":[202]},"counts_by_year":[{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}