{"id":"https://openalex.org/W2091120026","doi":"https://doi.org/10.1046/j.1365-2672.2002.01742.x","title":"Kytococcus sedentarius, the organism associated with pitted keratolysis, produces two keratin-degrading enzymes","display_name":"Kytococcus sedentarius, the organism associated with pitted keratolysis, produces two keratin-degrading enzymes","publication_year":2002,"publication_date":"2002-11-01","ids":{"openalex":"https://openalex.org/W2091120026","doi":"https://doi.org/10.1046/j.1365-2672.2002.01742.x","mag":"2091120026","pmid":"https://pubmed.ncbi.nlm.nih.gov/12392527"},"language":"en","primary_location":{"id":"doi:10.1046/j.1365-2672.2002.01742.x","doi":"https://doi.org/10.1046/j.1365-2672.2002.01742.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2672.2002.01742.x","pdf_url":null,"source":{"id":"https://openalex.org/S39794811","display_name":"Journal of Applied Microbiology","issn_l":"1364-5072","issn":["1364-5072","1365-2672"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016916593","display_name":"Christopher M. Longshaw","orcid":"https://orcid.org/0000-0002-5586-7256","observed_orcids":["https://orcid.org/0000-0002-5586-7256"]},"institutions":[{"id":"https://openalex.org/I130828816","display_name":"University of Leeds","ror":"https://ror.org/024mrxd33","country_code":"GB","type":"education","lineage":["https://openalex.org/I130828816"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"C.M. Longshaw","raw_affiliation_strings":["Skin Research Centre, Division of Microbiology, School of BMB, University of Leeds, Leeds, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Skin Research Centre, Division of Microbiology, School of BMB, University of Leeds, Leeds, UK","institution_ids":["https://openalex.org/I130828816"]}]},{"author_position":"middle","author":{"id":null,"display_name":"J.D. Wright","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130828816","display_name":"University of Leeds","ror":"https://ror.org/024mrxd33","country_code":"GB","type":"education","lineage":["https://openalex.org/I130828816"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"J.D. Wright","raw_affiliation_strings":["Skin Research Centre, Division of Microbiology, School of BMB, University of Leeds, Leeds, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Skin Research Centre, Division of Microbiology, School of BMB, University of Leeds, Leeds, UK","institution_ids":["https://openalex.org/I130828816"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054659472","display_name":"Angela M. Farrell","orcid":"https://orcid.org/0000-0001-5777-1613","observed_orcids":["https://orcid.org/0000-0001-5777-1613"]},"institutions":[{"id":"https://openalex.org/I130828816","display_name":"University of Leeds","ror":"https://ror.org/024mrxd33","country_code":"GB","type":"education","lineage":["https://openalex.org/I130828816"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"A.M. Farrell","raw_affiliation_strings":["Skin Research Centre, Division of Microbiology, School of BMB, University of Leeds, Leeds, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Skin Research Centre, Division of Microbiology, School of BMB, University of Leeds, Leeds, UK","institution_ids":["https://openalex.org/I130828816"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110172471","display_name":"K.T. Holland","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130828816","display_name":"University of Leeds","ror":"https://ror.org/024mrxd33","country_code":"GB","type":"education","lineage":["https://openalex.org/I130828816"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"K.T. Holland","raw_affiliation_strings":["Skin Research Centre, Division of Microbiology, School of BMB, University of Leeds, Leeds, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Skin Research Centre, Division of Microbiology, School of BMB, University of Leeds, Leeds, UK","institution_ids":["https://openalex.org/I130828816"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I130828816"],"apc_list":{"value":2864,"currency":"GBP","value_usd":3777},"apc_paid":null,"fwci":0.9119,"has_fulltext":false,"cited_by_count":76,"citation_normalized_percentile":{"value":0.70967742,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"93","issue":"5","first_page":"810","last_page":"816"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8901000022888184,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8901000022888184,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12116","display_name":"Dermatological diseases and infestations","score":0.01850000023841858,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11650","display_name":"Skin and Cellular Biology Research","score":0.006000000052154064,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/kytococcus-sedentarius","display_name":"Kytococcus sedentarius","score":1.0},{"id":"https://openalex.org/keywords/pitted-keratolysis","display_name":"pitted keratolysis","score":0.9990000128746033},{"id":"https://openalex.org/keywords/serine-protease","display_name":"serine protease","score":0.9359999895095825},{"id":"https://openalex.org/keywords/extracellular-enzymes","display_name":"extracellular enzymes","score":0.7630000114440918},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.6060000061988831},{"id":"https://openalex.org/keywords/cleavage-site","display_name":"cleavage site","score":0.6840000152587891}],"concepts":[{"id":"https://openalex.org/C81241287","wikidata":"https://www.wikidata.org/wiki/Q189474","display_name":"Keratin","level":2,"score":0.7342501878738403},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6743822693824768},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.6729039549827576},{"id":"https://openalex.org/C137858568","wikidata":"https://www.wikidata.org/wiki/Q7239","display_name":"Organism","level":2,"score":0.6420614719390869},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5103875994682312},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.23780179023742676},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0608290433883667}],"mesh":[{"descriptor_ui":"D000192","descriptor_name":"Actinomycetales","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D000192","descriptor_name":"Actinomycetales","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D005110","descriptor_name":"Extracellular Space","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D005110","descriptor_name":"Extracellular Space","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005533","descriptor_name":"Foot Dermatoses","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D005533","descriptor_name":"Foot Dermatoses","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007328","descriptor_name":"Insulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007633","descriptor_name":"Keratins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1046/j.1365-2672.2002.01742.x","doi":"https://doi.org/10.1046/j.1365-2672.2002.01742.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2672.2002.01742.x","pdf_url":null,"source":{"id":"https://openalex.org/S39794811","display_name":"Journal of Applied Microbiology","issn_l":"1364-5072","issn":["1364-5072","1365-2672"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Microbiology","raw_type":"journal-article"},{"id":"pmid:12392527","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12392527","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of applied microbiology","raw_type":"Journal Article"},{"id":"mag:3016566172","doi":null,"is_oa":false,"landing_page_url":"https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902114906033450&rel=0","pdf_url":null,"source":{"id":"https://openalex.org/S39794811","display_name":"Journal of Applied Microbiology","issn_l":"1364-5072","issn":["1364-5072","1365-2672"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Journal of Applied Microbiology","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W1516656431","https://openalex.org/W1793671699","https://openalex.org/W1900286161","https://openalex.org/W1983907518","https://openalex.org/W1992098996","https://openalex.org/W2005379740","https://openalex.org/W2013129766","https://openalex.org/W2074100637","https://openalex.org/W2078768347","https://openalex.org/W2091233037","https://openalex.org/W2100837269","https://openalex.org/W2102628698","https://openalex.org/W2105095624","https://openalex.org/W2144634347","https://openalex.org/W2175920363","https://openalex.org/W2208687009","https://openalex.org/W2314225431"],"related_works":["https://openalex.org/W2117824514","https://openalex.org/W2043521293","https://openalex.org/W3158619710","https://openalex.org/W1990209390","https://openalex.org/W2051451733","https://openalex.org/W1505724173","https://openalex.org/W2768902742","https://openalex.org/W3016566172","https://openalex.org/W1980033894","https://openalex.org/W1995653071"],"abstract_inverted_index":{"AIMS:":[0],"To":[1],"determine":[2],"characteristics":[3],"of":[4,9,20,26,38,56,93,127,163,170,202,217,247,253,256,266],"the":[5,125,168,226,234,251,264],"extracellular":[6,177],"enzyme":[7,43,150],"activity":[8,44,103,123],"Kytococcus":[10],"sedentarius":[11,174,215],"on":[12,269],"human":[13,184,203,223,237,270],"callus.":[14,185],"METHODS":[15],"AND":[16,206],"RESULTS:":[17],"A":[18],"concentrate":[19],"a":[21,36,200,254],"continuous":[22],"culture":[23],"supernatant":[24],"fluid":[25],"K.":[27,173,214],"sedentarius,":[28],"which":[29,220],"had":[30],"callus-degrading":[31,102],"activity,":[32],"was":[33,45],"subjected":[34],"to":[35,47,50,68],"series":[37],"chromatographic":[39],"purification":[40],"procedures.":[41],"The":[42,77,98,148,211],"found":[46],"be":[48,246],"attributable":[49],"two":[51,176,218],"proteases.":[52],"These":[53,243],"were":[54,66,82,104,135,153],"capable":[55],"degrading":[57],"both":[58,133],"native":[59],"callus":[60,224,268],"and":[61,65,80,85,95,112,132,143,157,165,191,262],"extracted":[62],"keratin":[63,257],"polypeptides":[64],"purified":[67],"homogeneity,":[69],"as":[70,159],"shown":[71],"by":[72,137],"SDS-PAGE":[73],"with":[74,90,167],"silver":[75],"staining.":[76],"enzymes":[78,134,178,187,219,244],"P1":[79,111,164],"P2":[81,120,166],"30":[83],"kDa":[84,87],"50":[86,113],"in":[88,124,199,213,236,240,250,263],"size":[89],"isoelectric":[91],"points":[92],"4.6":[94],"2.7,":[96],"respectively.":[97],"optimum":[99],"conditions":[100],"for":[101,110,118,233],"40":[105],"degrees":[106,114],"C,":[107,115],"pH":[108,116],"7.1":[109],"7.5":[117],"P2.":[119],"displayed":[121],"increased":[122],"presence":[126],"800":[128],"mmol":[129,145],"l(-1)":[130,146],"NaCl":[131],"inhibited":[136],"PMSF":[138],"(1":[139],"mmol(-1)":[140],"Phenylmethylsulphoryl":[141],"fluoride)":[142],"1":[144],"EDTA.":[147],"main":[149],"cleavage":[151,193],"sites":[152,195],"Lys-Trp,":[154],"Val-Lys,":[155],"Gly-Asp":[156],"Asp-Arg,":[158],"determined":[160],"after":[161],"incubation":[162],"beta-chain":[169],"insulin.":[171],"CONCLUSIONS:":[172],"produces":[175],"that":[179,196,228],"independently":[180],"degrade":[181,222],"natural,":[182],"insoluble":[183],"Both":[186],"are":[188,197],"serine":[189],"proteases":[190],"have":[192],"preference":[194],"present":[198],"range":[201,255],"keratins.":[204],"SIGNIFICANCE":[205],"IMPACT":[207],"OF":[208],"THE":[209],"STUDY:":[210],"identification,":[212],"cultures,":[216],"can":[221],"strengthens":[225],"hypothesis":[227],"this":[229],"organism":[230],"is":[231],"responsible":[232],"pitting":[235],"epidermis":[238],"observed":[239],"pitted":[241],"keratolysis.":[242],"may":[245],"commercial":[248],"use":[249],"biodegradation":[252],"polymers,":[258],"biological":[259],"washing":[260],"powders":[261],"treatment":[265],"unwanted":[267],"skin.":[271]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":9}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}
