{"id":"https://openalex.org/W2090535332","doi":"https://doi.org/10.1073/pnas.81.20.6295","title":"Isolation and characterization of a cDNA clone for bovine cytochrome c oxidase subunit IV.","display_name":"Isolation and characterization of a cDNA clone for bovine cytochrome c oxidase subunit IV.","publication_year":1984,"publication_date":"1984-10-01","ids":{"openalex":"https://openalex.org/W2090535332","doi":"https://doi.org/10.1073/pnas.81.20.6295","mag":"2090535332","pmid":"https://pubmed.ncbi.nlm.nih.gov/6093095","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/391910"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.81.20.6295","doi":"https://doi.org/10.1073/pnas.81.20.6295","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.81.20.6295","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/391910","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090217887","display_name":"Margaret I. Lomax","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M I Lomax","raw_affiliation_strings":["Kresge Hearing Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kresge Hearing Research Institute","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050524011","display_name":"Nancy J. Bachman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"N J Bachman","raw_affiliation_strings":["(University of Michigan)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"(University of Michigan)","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034090271","display_name":"Marc S. Nasoff","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M S Nasoff","raw_affiliation_strings":["(University of Michigan)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"(University of Michigan)","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027707988","display_name":"Marvin H. Caruthers","orcid":"https://orcid.org/0000-0002-4931-468X","observed_orcids":["https://orcid.org/0000-0002-4931-468X"]},"institutions":[{"id":"https://openalex.org/I188538660","display_name":"University of Colorado Boulder","ror":"https://ror.org/02ttsq026","country_code":"US","type":"education","lineage":["https://openalex.org/I188538660"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M H Caruthers","raw_affiliation_strings":["Department of Cellular and Molecular Biology, Division of Biological Sciences, Chemistry, The University of Colorado, Boulder, CO 80309"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cellular and Molecular Biology, Division of Biological Sciences, Chemistry, The University of Colorado, Boulder, CO 80309","institution_ids":["https://openalex.org/I188538660"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5026416444","display_name":"Lawrence I. Grossman","orcid":"https://orcid.org/0000-0002-1059-9358","observed_orcids":["https://orcid.org/0000-0002-1059-9358"]},"institutions":[{"id":"https://openalex.org/I188538660","display_name":"University of Colorado Boulder","ror":"https://ror.org/02ttsq026","country_code":"US","type":"education","lineage":["https://openalex.org/I188538660"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"L I Grossman","raw_affiliation_strings":["Department of Cellular and Molecular Biology, Division of Biological Sciences, Chemistry, The University of Colorado, Boulder, CO 80309"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cellular and Molecular Biology, Division of Biological Sciences, Chemistry, The University of Colorado, Boulder, CO 80309","institution_ids":["https://openalex.org/I188538660"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.9332,"has_fulltext":false,"cited_by_count":57,"citation_normalized_percentile":{"value":0.92305794,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":null,"biblio":{"volume":"81","issue":"20","first_page":"6295","last_page":"6299"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.9603999853134155,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.9603999853134155,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.00860000029206276,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.005499999970197678,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cytochrome-c-oxidase","display_name":"cytochrome c oxidase","score":0.9729999899864197},{"id":"https://openalex.org/keywords/subunit-iv","display_name":"subunit IV","score":0.9860000014305115},{"id":"https://openalex.org/keywords/cattle","display_name":"cattle","score":0.7179999947547913},{"id":"https://openalex.org/keywords/cdna-cloning","display_name":"cDNA cloning","score":0.9089999794960022},{"id":"https://openalex.org/keywords/mitochondrial-presequence","display_name":"mitochondrial presequence","score":0.6629999876022339},{"id":"https://openalex.org/keywords/signal-peptide","display_name":"signal peptide","score":0.5059999823570251},{"id":"https://openalex.org/keywords/protein-processing","display_name":"protein processing","score":0.4020000100135803},{"id":"https://openalex.org/keywords/bovine-liver","display_name":"bovine liver","score":0.5080000162124634},{"id":"https://openalex.org/keywords/mitochondrial-protein-targeting","display_name":"mitochondrial protein targeting","score":0.5049999952316284},{"id":"https://openalex.org/keywords/amino-acid-sequence","display_name":"amino acid sequence","score":0.5709999799728394}],"concepts":[{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.8065401315689087},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7085127830505371},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.6265605092048645},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.6083654761314392},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6060219407081604},{"id":"https://openalex.org/C189482833","wikidata":"https://www.wikidata.org/wiki/Q869146","display_name":"cDNA library","level":4,"score":0.5610100030899048},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5574994683265686},{"id":"https://openalex.org/C10858879","wikidata":"https://www.wikidata.org/wiki/Q899790","display_name":"Signal peptide","level":4,"score":0.541192889213562},{"id":"https://openalex.org/C84148353","wikidata":"https://www.wikidata.org/wiki/Q863908","display_name":"Nucleic acid sequence","level":3,"score":0.5258805751800537},{"id":"https://openalex.org/C92825506","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Protein primary structure","level":4,"score":0.5107324123382568},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.5057437419891357},{"id":"https://openalex.org/C123249941","wikidata":"https://www.wikidata.org/wiki/Q306116","display_name":"Cytochrome c oxidase","level":3,"score":0.45367735624313354},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.2557194232940674},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2018682062625885},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.14173880219459534}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003576","descriptor_name":"Electron Transport Complex IV","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011498","descriptor_name":"Protein Precursors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1073/pnas.81.20.6295","doi":"https://doi.org/10.1073/pnas.81.20.6295","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.81.20.6295","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:391910","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/391910","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmid:6093095","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6093095","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:391910","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/391910","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3737562089","display_name":"Molecular Evolution of Cytochrome C Oxidase in Primates","funder_award_id":"8118915","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320309652","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":42,"referenced_works":["https://openalex.org/W37553100","https://openalex.org/W1489237559","https://openalex.org/W1514803684","https://openalex.org/W1535064294","https://openalex.org/W1540379142","https://openalex.org/W1541569010","https://openalex.org/W1555451635","https://openalex.org/W1558516755","https://openalex.org/W1586195802","https://openalex.org/W1618030109","https://openalex.org/W1620974602","https://openalex.org/W1676746134","https://openalex.org/W1981843245","https://openalex.org/W2007559564","https://openalex.org/W2009116733","https://openalex.org/W2023562175","https://openalex.org/W2027966021","https://openalex.org/W2030834306","https://openalex.org/W2033536030","https://openalex.org/W2035776880","https://openalex.org/W2037457710","https://openalex.org/W2049315873","https://openalex.org/W2060333964","https://openalex.org/W2063450941","https://openalex.org/W2084630904","https://openalex.org/W2091079493","https://openalex.org/W2091467829","https://openalex.org/W2092065468","https://openalex.org/W2103946966","https://openalex.org/W2109291482","https://openalex.org/W2120202917","https://openalex.org/W2138270253","https://openalex.org/W2139575182","https://openalex.org/W2141100976","https://openalex.org/W2158182001","https://openalex.org/W2173897585","https://openalex.org/W2197196092","https://openalex.org/W2326306921","https://openalex.org/W2415666558","https://openalex.org/W2424605675","https://openalex.org/W2764264365","https://openalex.org/W4302311430"],"related_works":["https://openalex.org/W3155768904","https://openalex.org/W2059834781","https://openalex.org/W2260884575","https://openalex.org/W2417909860","https://openalex.org/W1967295789","https://openalex.org/W1979749697","https://openalex.org/W2048465856","https://openalex.org/W2037600698","https://openalex.org/W2060946025","https://openalex.org/W4213030135"],"abstract_inverted_index":{"We":[0,56,86],"have":[1],"isolated":[2],"a":[3,61,139,196],"cDNA":[4,23,37,78],"clone":[5],"for":[6,102,199],"the":[7,39,88,92,99,107,121,127,132,135,148,151,166],"precursor":[8],"to":[9,66,173],"subunit":[10,81],"IV":[11,82],"of":[12,30,36,52,63,91,106,120,134,150],"bovine":[13],"cytochrome":[14],"c":[15],"oxidase":[16],"(ferrocytochrome":[17],"c:oxygen":[18],"oxidoreductase,":[19],"EC":[20],"1.9.3.1).":[21],"A":[22],"library":[24],"was":[25],"constructed":[26],"from":[27,147],"poly(A)+":[28],"RNA":[29],"adult":[31],"beef":[32],"liver":[33],"by":[34,46],"insertion":[35],"into":[38],"plasmid":[40],"vector":[41],"pBR322.":[42],"Transformants":[43],"were":[44],"screened":[45,57],"colony":[47],"hybridization":[48],"with":[49,60,126,188],"two":[50,77],"mixtures":[51],"[32P]-labeled":[53],"synthetic":[54],"oligodeoxyribonucleotides.":[55],"20,000":[58],"transformants":[59],"mixture":[62],"heptadecamers":[64],"complementary":[65],"all":[67],"16":[68],"possible":[69],"sequences":[70],"encoding":[71,80],"amino":[72,83,103,117,144],"acids":[73,104],"98-103":[74],"and":[75,110,159,178,202],"obtained":[76],"clones":[79],"acid":[84,118,145],"sequences.":[85],"determined":[87,129],"DNA":[89],"sequence":[90,101,119,133],"larger":[93],"(416":[94],"base-pair)":[95],"insert,":[96],"which":[97,193],"contains":[98,138,160],"coding":[100],"1-107":[105],"mature":[108,122],"protein":[109,123,130,191],"an":[111],"NH2-terminal":[112,136],"extension":[113,137],"(presequence).":[114],"The":[115,154],"deduced":[116],"is":[124,156,182],"identical":[125],"previously":[128],"sequence:":[131],"potential":[140],"initiator":[141],"methionine":[142],"at":[143,165],"-22":[146],"NH2-terminus":[149],"processed":[152],"protein.":[153],"presequence":[155],"quite":[157],"basic":[158],"several":[161],"arginines,":[162],"including":[163],"one":[164],"processing":[167],"site.":[168],"No":[169],"hydrophobic":[170],"region":[171],"analogous":[172],"that":[174],"found":[175],"in":[176],"bacterial":[177],"eukaryotic":[179],"signal":[180,198],"peptides":[181],"present,":[183],"but":[184],"there":[185],"are":[186],"homologies":[187],"other":[189],"mitochondrial":[190],"presequences,":[192],"may":[194],"include":[195],"common":[197],"their":[200],"destination":[201],"processing.":[203]},"counts_by_year":[],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2016-06-24T00:00:00"}