{"id":"https://openalex.org/W2089655412","doi":"https://doi.org/10.1264/jsme2.me10172","title":"Effect of Tris-HCl Buffer on DNA Adsorption by a Variety of Soil Constituents","display_name":"Effect of Tris-HCl Buffer on DNA Adsorption by a Variety of Soil Constituents","publication_year":2010,"publication_date":"2010-12-17","ids":{"openalex":"https://openalex.org/W2089655412","doi":"https://doi.org/10.1264/jsme2.me10172","mag":"2089655412","pmid":"https://pubmed.ncbi.nlm.nih.gov/21487209"},"language":"en","primary_location":{"id":"doi:10.1264/jsme2.me10172","doi":"https://doi.org/10.1264/jsme2.me10172","is_oa":true,"landing_page_url":"https://doi.org/10.1264/jsme2.me10172","pdf_url":"https://www.jstage.jst.go.jp/article/jsme2/26/1/26_ME10172/_pdf","source":{"id":"https://openalex.org/S170963199","display_name":"Microbes and Environments","issn_l":"1342-6311","issn":["1342-6311","1347-4405"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4366371044","host_organization_name":"Japanese Society Of Microbial Ecology","host_organization_lineage":["https://openalex.org/P4366371044"],"host_organization_lineage_names":["Japanese Society Of Microbial Ecology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbes and Environments","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jstage.jst.go.jp/article/jsme2/26/1/26_ME10172/_pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109189413","display_name":"Kazutoshi Saeki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135598925","display_name":"Kyushu University","ror":"https://ror.org/00p4k0j84","country_code":"JP","type":"education","lineage":["https://openalex.org/I135598925"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kazutoshi Saeki","raw_affiliation_strings":["Biotron Institute, Kyushu University","Biotron Institute, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotron Institute, Kyushu University","institution_ids":["https://openalex.org/I135598925"]},{"raw_affiliation_string":"Biotron Institute, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan","institution_ids":["https://openalex.org/I135598925"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072324087","display_name":"Takashi Kunito","orcid":"https://orcid.org/0000-0002-3028-736X","observed_orcids":["https://orcid.org/0000-0002-3028-736X"]},"institutions":[{"id":"https://openalex.org/I137975476","display_name":"Shinshu University","ror":"https://ror.org/0244rem06","country_code":"JP","type":"education","lineage":["https://openalex.org/I137975476"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takashi Kunito","raw_affiliation_strings":["Faculty of Science, Shinshu University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Science, Shinshu University","institution_ids":["https://openalex.org/I137975476"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109466766","display_name":"Masao Sakai","orcid":"https://orcid.org/0009-0005-6161-8773","observed_orcids":["https://orcid.org/0009-0005-6161-8773"]},"institutions":[{"id":"https://openalex.org/I107139324","display_name":"Kagoshima University","ror":"https://ror.org/03ss88z23","country_code":"JP","type":"education","lineage":["https://openalex.org/I107139324"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Masao Sakai","raw_affiliation_strings":["Faculty of Agriculture, Kagoshima University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Agriculture, Kagoshima University","institution_ids":["https://openalex.org/I107139324"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.5844,"has_fulltext":true,"cited_by_count":31,"citation_normalized_percentile":{"value":0.83428167,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"26","issue":"1","first_page":"88","last_page":"91"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13793","display_name":"Environmental and Analytical Chemistry Studies","score":0.2531999945640564,"subfield":{"id":"https://openalex.org/subfields/2312","display_name":"Water Science and Technology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T13793","display_name":"Environmental and Analytical Chemistry Studies","score":0.2531999945640564,"subfield":{"id":"https://openalex.org/subfields/2312","display_name":"Water Science and Technology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11852","display_name":"Clay minerals and soil interactions","score":0.1459999978542328,"subfield":{"id":"https://openalex.org/subfields/1906","display_name":"Geochemistry and Petrology"},"field":{"id":"https://openalex.org/fields/19","display_name":"Earth and Planetary Sciences"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10419","display_name":"Pharmaceutical and Antibiotic Environmental Impacts","score":0.05249999836087227,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-adsorption","display_name":"DNA adsorption","score":0.902999997138977},{"id":"https://openalex.org/keywords/tris-hcl-buffer","display_name":"Tris-HCl buffer","score":0.9610000252723694},{"id":"https://openalex.org/keywords/soil-components","display_name":"soil components","score":0.6650000214576721},{"id":"https://openalex.org/keywords/gibbsite","display_name":"gibbsite","score":0.9440000057220459},{"id":"https://openalex.org/keywords/goethite","display_name":"goethite","score":0.9980000257492065},{"id":"https://openalex.org/keywords/montmorillonite","display_name":"montmorillonite","score":1.0},{"id":"https://openalex.org/keywords/kaolinite","display_name":"kaolinite","score":1.0},{"id":"https://openalex.org/keywords/allophane","display_name":"allophane","score":0.9049999713897705},{"id":"https://openalex.org/keywords/humic-acid","display_name":"humic acid","score":0.8840000033378601},{"id":"https://openalex.org/keywords/volcanic-ash-soil","display_name":"volcanic ash soil","score":0.9909999966621399},{"id":"https://openalex.org/keywords/extracellular-dna","display_name":"extracellular DNA","score":0.7860000133514404}],"concepts":[{"id":"https://openalex.org/C2781014771","wikidata":"https://www.wikidata.org/wiki/Q413961","display_name":"Tris","level":2,"score":0.8686954975128174},{"id":"https://openalex.org/C2778973568","wikidata":"https://www.wikidata.org/wiki/Q429712","display_name":"Allophane","level":3,"score":0.8172228932380676},{"id":"https://openalex.org/C2777975867","wikidata":"https://www.wikidata.org/wiki/Q408516","display_name":"Gibbsite","level":3,"score":0.7795746326446533},{"id":"https://openalex.org/C2779899878","wikidata":"https://www.wikidata.org/wiki/Q223197","display_name":"Kaolinite","level":2,"score":0.7419401407241821},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7358070611953735},{"id":"https://openalex.org/C2777515222","wikidata":"https://www.wikidata.org/wiki/Q422131","display_name":"Montmorillonite","level":2,"score":0.7346392869949341},{"id":"https://openalex.org/C150394285","wikidata":"https://www.wikidata.org/wiki/Q180254","display_name":"Adsorption","level":2,"score":0.689081072807312},{"id":"https://openalex.org/C2780591741","wikidata":"https://www.wikidata.org/wiki/Q899405","display_name":"Hydroxymethyl","level":2,"score":0.6275108456611633},{"id":"https://openalex.org/C2777787761","wikidata":"https://www.wikidata.org/wiki/Q189703","display_name":"Goethite","level":3,"score":0.5479394197463989},{"id":"https://openalex.org/C179104552","wikidata":"https://www.wikidata.org/wiki/Q11165","display_name":"Inorganic chemistry","level":1,"score":0.5463503003120422},{"id":"https://openalex.org/C13965031","wikidata":"https://www.wikidata.org/wiki/Q243545","display_name":"Nuclear chemistry","level":1,"score":0.46149951219558716},{"id":"https://openalex.org/C2778301344","wikidata":"https://www.wikidata.org/wiki/Q492763","display_name":"Andosol","level":3,"score":0.43441373109817505},{"id":"https://openalex.org/C2780965034","wikidata":"https://www.wikidata.org/wiki/Q4152088","display_name":"Humic acid","level":3,"score":0.4239423871040344},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.21616002917289734},{"id":"https://openalex.org/C199289684","wikidata":"https://www.wikidata.org/wiki/Q83353","display_name":"Mineralogy","level":1,"score":0.13891145586967468},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12498870491981506},{"id":"https://openalex.org/C159750122","wikidata":"https://www.wikidata.org/wiki/Q96621023","display_name":"Soil water","level":2,"score":0.10958424210548401},{"id":"https://openalex.org/C127313418","wikidata":"https://www.wikidata.org/wiki/Q1069","display_name":"Geology","level":0,"score":0.06929594278335571},{"id":"https://openalex.org/C2780560099","wikidata":"https://www.wikidata.org/wiki/Q83323","display_name":"Fertilizer","level":2,"score":0.06877043843269348},{"id":"https://openalex.org/C159390177","wikidata":"https://www.wikidata.org/wiki/Q9161265","display_name":"Soil science","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000327","descriptor_name":"Adsorption","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002021","descriptor_name":"Buffers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006812","descriptor_name":"Humic Substances","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D006851","descriptor_name":"Hydrochloric Acid","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012987","descriptor_name":"Soil","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D014325","descriptor_name":"Tromethamine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1264/jsme2.me10172","doi":"https://doi.org/10.1264/jsme2.me10172","is_oa":true,"landing_page_url":"https://doi.org/10.1264/jsme2.me10172","pdf_url":"https://www.jstage.jst.go.jp/article/jsme2/26/1/26_ME10172/_pdf","source":{"id":"https://openalex.org/S170963199","display_name":"Microbes and Environments","issn_l":"1342-6311","issn":["1342-6311","1347-4405"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4366371044","host_organization_name":"Japanese Society Of Microbial Ecology","host_organization_lineage":["https://openalex.org/P4366371044"],"host_organization_lineage_names":["Japanese Society Of Microbial Ecology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbes and Environments","raw_type":"journal-article"},{"id":"pmid:21487209","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21487209","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbes and environments","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1264/jsme2.me10172","doi":"https://doi.org/10.1264/jsme2.me10172","is_oa":true,"landing_page_url":"https://doi.org/10.1264/jsme2.me10172","pdf_url":"https://www.jstage.jst.go.jp/article/jsme2/26/1/26_ME10172/_pdf","source":{"id":"https://openalex.org/S170963199","display_name":"Microbes and Environments","issn_l":"1342-6311","issn":["1342-6311","1347-4405"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4366371044","host_organization_name":"Japanese Society Of Microbial Ecology","host_organization_lineage":["https://openalex.org/P4366371044"],"host_organization_lineage_names":["Japanese Society Of Microbial Ecology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbes and Environments","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G7101610931","display_name":"\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048\u4f5c\u7269\u4e2dDNA\u5206\u5b50\u306e\u571f\u58cc\u751f\u614b\u7cfb\u306b\u304a\u3051\u308b\u6319\u52d5\u30fb\u5f71\u97ff\u3092\u63a2\u308b","funder_award_id":"22380180","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2089655412.pdf","grobid_xml":"https://content.openalex.org/works/W2089655412.grobid-xml"},"referenced_works_count":36,"referenced_works":["https://openalex.org/W1537049479","https://openalex.org/W1541416380","https://openalex.org/W1580604153","https://openalex.org/W1608514573","https://openalex.org/W1965915467","https://openalex.org/W1969254808","https://openalex.org/W1969489621","https://openalex.org/W1973134066","https://openalex.org/W1974058293","https://openalex.org/W1974706038","https://openalex.org/W1977229826","https://openalex.org/W1987416145","https://openalex.org/W1990652653","https://openalex.org/W1994213294","https://openalex.org/W2001707556","https://openalex.org/W2009680009","https://openalex.org/W2020791420","https://openalex.org/W2030397253","https://openalex.org/W2049373241","https://openalex.org/W2049536449","https://openalex.org/W2054967551","https://openalex.org/W2061058752","https://openalex.org/W2061186101","https://openalex.org/W2061291107","https://openalex.org/W2061891046","https://openalex.org/W2071299484","https://openalex.org/W2077013098","https://openalex.org/W2079928468","https://openalex.org/W2083100436","https://openalex.org/W2089130260","https://openalex.org/W2094548612","https://openalex.org/W2123886059","https://openalex.org/W2125392741","https://openalex.org/W2316131531","https://openalex.org/W2463527057","https://openalex.org/W2513006702"],"related_works":["https://openalex.org/W1969254808","https://openalex.org/W1987416145","https://openalex.org/W2017354194","https://openalex.org/W2049536449","https://openalex.org/W1973134066","https://openalex.org/W2280019096","https://openalex.org/W2382374220","https://openalex.org/W2399110276","https://openalex.org/W2030397253","https://openalex.org/W2079928468"],"abstract_inverted_index":{"We":[0],"investigated":[1],"the":[2,16,70,86],"effect":[3],"of":[4,18,88],"tris(hydroxymethyl)aminomethane":[5],"hydrochloride":[6],"(Tris-HCl)":[7],"buffer":[8,52],"(pH":[9],"7.0)":[10],"as":[11],"a":[12,48,55],"bulk":[13],"solution":[14,59,74],"on":[15,85],"adsorption":[17,87],"DNA":[19,46,68,90],"by":[20,92],"gibbsite,":[21,38],"goethite,":[22],"montmorillonite,":[23],"kaolinite,":[24],"synthetic":[25],"and":[26,32,40],"natural":[27,36],"allophanes,":[28],"two":[29,33],"humic":[30],"acids":[31],"andosols.":[34],"The":[35],"allophane,":[37],"kaolinite":[39],"an":[41],"andosol":[42],"adsorbed":[43,65],"significantly":[44,66],"more":[45],"in":[47,54,69,83],"0.1":[49,56],"M":[50,57],"Tris-HCl":[51,71,82],"than":[53,72],"NaCl":[58,73],"(t-test,":[60],"P<0.005).":[61],"In":[62],"contrast,":[63],"montmorillonite":[64],"less":[67],"(P<0.05).":[75],"Care":[76],"should":[77],"be":[78],"taken":[79],"when":[80],"using":[81],"studies":[84],"extracellular":[89],"molecules":[91],"soil":[93],"particles.":[94]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":4},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":3}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}