{"id":"https://openalex.org/W2088263183","doi":"https://doi.org/10.1111/j.1471-4159.1977.tb07711.x","title":"PRELIMINARY ESTIMATES OF THE DOPAMINE \u03b2\u2010HYDROXYLASE CONTENT AND ACTIVITY IN PURIFIED NORADRENERGIC VESICLES1","display_name":"PRELIMINARY ESTIMATES OF THE DOPAMINE \u03b2\u2010HYDROXYLASE CONTENT AND ACTIVITY IN PURIFIED NORADRENERGIC VESICLES1","publication_year":1977,"publication_date":"1977-01-01","ids":{"openalex":"https://openalex.org/W2088263183","doi":"https://doi.org/10.1111/j.1471-4159.1977.tb07711.x","mag":"2088263183","pmid":"https://pubmed.ncbi.nlm.nih.gov/833606"},"language":"en","primary_location":{"id":"doi:10.1111/j.1471-4159.1977.tb07711.x","doi":"https://doi.org/10.1111/j.1471-4159.1977.tb07711.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1471-4159.1977.tb07711.x","pdf_url":null,"source":{"id":"https://openalex.org/S135837084","display_name":"Journal of Neurochemistry","issn_l":"0022-3042","issn":["0022-3042","1471-4159"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Neurochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5064715409","display_name":"Richard L. Klein","orcid":"https://orcid.org/0000-0002-0316-206X","observed_orcids":["https://orcid.org/0000-0002-0316-206X"]},"institutions":[{"id":"https://openalex.org/I29606459","display_name":"University of Mississippi Medical Center","ror":"https://ror.org/044pcn091","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I29606459","https://openalex.org/I4210141039"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R. L. Klein","raw_affiliation_strings":["Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, MS 39216, U. S. A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, MS 39216, U. S. A","institution_ids":["https://openalex.org/I29606459"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085225653","display_name":"Donny F. Kirksey","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I29606459","display_name":"University of Mississippi Medical Center","ror":"https://ror.org/044pcn091","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I29606459","https://openalex.org/I4210141039"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D. F. Kirksey","raw_affiliation_strings":["Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, MS 39216, U. S. A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, MS 39216, U. S. A","institution_ids":["https://openalex.org/I29606459"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112679626","display_name":"R.A. Rush","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I169541294","display_name":"Flinders University","ror":"https://ror.org/01kpzv902","country_code":"AU","type":"education","lineage":["https://openalex.org/I169541294"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"R. A. Rush","raw_affiliation_strings":["Flinders University School of Medicine, Bedford Park, So. Australia 5042"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Flinders University School of Medicine, Bedford Park, So. Australia 5042","institution_ids":["https://openalex.org/I169541294"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109808478","display_name":"Menek Goldstein","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210086933","display_name":"NYU Langone Health","ror":"https://ror.org/005dvqh91","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210086933"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. Goldstein","raw_affiliation_strings":["Department of Neurochemistry, NYU Medical Center, New York, NY 10016, U. S. A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurochemistry, NYU Medical Center, New York, NY 10016, U. S. A","institution_ids":["https://openalex.org/I4210086933"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":5330,"currency":"USD","value_usd":5330},"apc_paid":null,"fwci":3.086,"has_fulltext":false,"cited_by_count":27,"citation_normalized_percentile":{"value":0.92003008,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":null,"biblio":{"volume":"28","issue":"1","first_page":"81","last_page":"86"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.47360000014305115,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.47360000014305115,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.34940001368522644,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10056","display_name":"Neurotransmitter Receptor Influence on Behavior","score":0.014999999664723873,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dopamine-beta-hydroxylase","display_name":"dopamine beta-hydroxylase","score":0.984000027179718},{"id":"https://openalex.org/keywords/dense-core-vesicles","display_name":"dense core vesicles","score":0.8740000128746033},{"id":"https://openalex.org/keywords/enzyme-activity","display_name":"enzyme activity","score":0.6060000061988831},{"id":"https://openalex.org/keywords/enzyme-content","display_name":"enzyme content","score":0.5490000247955322},{"id":"https://openalex.org/keywords/latent-enzyme-activity","display_name":"latent enzyme activity","score":0.6769999861717224},{"id":"https://openalex.org/keywords/triton-x-100","display_name":"Triton X-100","score":0.8349999785423279},{"id":"https://openalex.org/keywords/vesicle-protein-composition","display_name":"vesicle protein composition","score":0.5680000185966492},{"id":"https://openalex.org/keywords/noradrenaline-synthesis","display_name":"noradrenaline synthesis","score":0.843999981880188},{"id":"https://openalex.org/keywords/norepinephrine-synthesis","display_name":"norepinephrine synthesis","score":0.9810000061988831},{"id":"https://openalex.org/keywords/exocytosis","display_name":"exocytosis","score":0.8809999823570251},{"id":"https://openalex.org/keywords/quantal-release","display_name":"quantal release","score":0.7639999985694885},{"id":"https://openalex.org/keywords/splenic-nerve","display_name":"splenic nerve","score":0.828000009059906},{"id":"https://openalex.org/keywords/cattle","display_name":"cattle","score":0.7910000085830688}],"concepts":[{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.8888741731643677},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5984057188034058},{"id":"https://openalex.org/C176685330","wikidata":"https://www.wikidata.org/wiki/Q857638","display_name":"Radioimmunoassay","level":2,"score":0.5887501239776611},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5729696750640869},{"id":"https://openalex.org/C513476851","wikidata":"https://www.wikidata.org/wiki/Q170304","display_name":"Dopamine","level":2,"score":0.47980478405952454},{"id":"https://openalex.org/C2778514916","wikidata":"https://www.wikidata.org/wiki/Q186242","display_name":"Norepinephrine","level":3,"score":0.46970197558403015},{"id":"https://openalex.org/C198684833","wikidata":"https://www.wikidata.org/wiki/Q2823748","display_name":"Specific activity","level":3,"score":0.461139053106308},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.42344802618026733},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.41477638483047485},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3919965326786041},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.26109957695007324},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24263769388198853},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.12014734745025635}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001338","descriptor_name":"Autonomic Fibers, Postganglionic","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004299","descriptor_name":"Dopamine beta-Hydroxylase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D004299","descriptor_name":"Dopamine beta-Hydroxylase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004299","descriptor_name":"Dopamine beta-Hydroxylase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009638","descriptor_name":"Norepinephrine","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011092","descriptor_name":"Polyethylene Glycols","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013154","descriptor_name":"Spleen","qualifier_ui":"Q000294","qualifier_name":"innervation","is_major_topic":false},{"descriptor_ui":"D013572","descriptor_name":"Synaptic Vesicles","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013572","descriptor_name":"Synaptic Vesicles","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1471-4159.1977.tb07711.x","doi":"https://doi.org/10.1111/j.1471-4159.1977.tb07711.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1471-4159.1977.tb07711.x","pdf_url":null,"source":{"id":"https://openalex.org/S135837084","display_name":"Journal of Neurochemistry","issn_l":"0022-3042","issn":["0022-3042","1471-4159"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Neurochemistry","raw_type":"journal-article"},{"id":"pmid:833606","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/833606","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of neurochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W67147141","https://openalex.org/W1775749144","https://openalex.org/W1906726181","https://openalex.org/W1964865241","https://openalex.org/W1973373401","https://openalex.org/W1980707436","https://openalex.org/W1983499679","https://openalex.org/W1989563572","https://openalex.org/W1993513520","https://openalex.org/W2003859441","https://openalex.org/W2012546637","https://openalex.org/W2014870763","https://openalex.org/W2015468241","https://openalex.org/W2017547502","https://openalex.org/W2024941240","https://openalex.org/W2025065965","https://openalex.org/W2025288480","https://openalex.org/W2032129123","https://openalex.org/W2035516849","https://openalex.org/W2043118285","https://openalex.org/W2044425637","https://openalex.org/W2093802813","https://openalex.org/W2094895198","https://openalex.org/W2098069077","https://openalex.org/W2102923474","https://openalex.org/W2132204205","https://openalex.org/W2133068304","https://openalex.org/W2217275303","https://openalex.org/W2223862935","https://openalex.org/W2233707407","https://openalex.org/W2337938824","https://openalex.org/W4235459369"],"related_works":["https://openalex.org/W2069486264","https://openalex.org/W2091232045","https://openalex.org/W2029864304","https://openalex.org/W1980707436","https://openalex.org/W2014364530","https://openalex.org/W2460535944","https://openalex.org/W31102057","https://openalex.org/W2020213222","https://openalex.org/W1999236437","https://openalex.org/W2021002424"],"abstract_inverted_index":{"Abstract\u2014":[0],"The":[1,126,153,179,214],"dopamine":[2],"\u03b2\u2010hydroxylase":[3],"(D\u03b2H)":[4],"content":[5],"and":[6,33,79,112,135,172,232],"activity":[7,26,35,52,80],"of":[8,48,101,105,121,133,139,161,165,175,210,236,242,248,258],"large":[9,226],"dense\u2010core":[10,227],"noradrenergic":[11],"vesicles":[12],"purified":[13],"from":[14,82,224],"bovine":[15],"splenic":[16],"nerve":[17],"were":[18,186],"determined":[19],"using":[20,149],"two":[21,151],"assay":[22,95],"procedures":[23],":":[24],"enzymic":[25],"expressed":[27,39],"in":[28,40,55,145,182],"Units":[29,41,86],"per":[30,42,87,163,177,212],"mg":[31,43,88],"protein":[32,109],"homospecific":[34,128],"based":[36,92,190],"on":[37,93,191],"radioimmunoassay":[38],"D\u03b2H":[44,67,111,166,211,218],"antigen.":[45],"Approximately":[46],"two\u2010thirds":[47],"the":[49,106,114,140,146,150,183,192,225,233,237,240,259],"total":[50,107],"enzyme":[51,122,142],"is":[53,196,230],"latent":[54],"these":[56],"vesicles,":[57],"even":[58],"after":[59],"various":[60,147,184],"treatments":[61],"designed":[62],"to":[63,84,158,199,255],"compromise":[64],"vesicle":[65,89,108,116,141,154,228,243,260],"integrity.":[66],"can":[68,155],"be":[69,200,205],"completely":[70],"unmasked":[71],"by":[72],"brief":[73],"treatment":[74],"with":[75],"0.01\u20100.05%":[76],"Triton":[77],"X\u2010100":[78],"increases":[81],"0.20":[83],"0.64":[85],"protein.":[90],"Calculations":[91],"both":[94],"methods":[96,193],"suggested":[97],"that":[98,113,202,216],"an":[99,131],"average":[100,115,132],"7%":[102],"(range":[103,123,137],"3\u201015%)":[104],"was":[110,143],"contained":[117],"about":[118],"4":[119],"molecules":[120,160,174,209],"2\u20109":[124],"molecules).":[125],"estimated":[127],"activities":[129],"indicated":[130],"25":[134],"50%":[136],"18\u201072%)":[138],"inactive":[144],"samples":[148],"antibodies.":[152],"synthesize":[156],"up":[157],"30":[159],"noradrenaline/s":[162],"molecule":[164],"at":[167],"near":[168],"optimal":[169],"substrate":[170],"concentration,":[171],"60\u2010270":[173],"norepinephrine/s":[176],"vesicle.":[178,213],"assumptions":[180],"used":[181],"calculations":[185],"critically":[187],"analyzed":[188],"and,":[189],"employed,":[194],"it":[195],"tentatively":[197],"considered":[198,231],"unlikely":[201],"there":[203],"could":[204],"more":[206],"than":[207],"5\u201012":[208],"possibility":[215],"circulating":[217],"originates":[219],"primarily,":[220],"if":[221],"not":[222],"exclusively,":[223],"type":[229],"functional":[234],"implications":[235],"data":[238],"support":[239],"concept":[241],"reuse":[244],"during":[245],"several":[246],"cycles":[247],"exocytosis":[249],"involving":[250],"a":[251,256],"quantal":[252],"size":[253],"equal":[254],"fraction":[257],"transmitter":[261],"content.":[262]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}