{"id":"https://openalex.org/W2088090898","doi":"https://doi.org/10.1073/pnas.81.18.5714","title":"Restriction endonucleases can be used to study B-Z junctions in supercoiled DNA.","display_name":"Restriction endonucleases can be used to study B-Z junctions in supercoiled DNA.","publication_year":1984,"publication_date":"1984-09-01","ids":{"openalex":"https://openalex.org/W2088090898","doi":"https://doi.org/10.1073/pnas.81.18.5714","mag":"2088090898","pmid":"https://pubmed.ncbi.nlm.nih.gov/6091104","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/391781"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.81.18.5714","doi":"https://doi.org/10.1073/pnas.81.18.5714","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.81.18.5714","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/391781","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5007262112","display_name":"Fernando Azor\u0131\u0301n","orcid":"https://orcid.org/0000-0002-8426-7858","observed_orcids":["https://orcid.org/0000-0002-8426-7858"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"F Azorin","raw_affiliation_strings":["Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":null,"display_name":"R Hahn","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R Hahn","raw_affiliation_strings":["Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103066840","display_name":"Alexander Rich","orcid":"https://orcid.org/0000-0002-1951-1180","observed_orcids":["https://orcid.org/0000-0002-1951-1180"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A Rich","raw_affiliation_strings":["Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139","institution_ids":["https://openalex.org/I63966007"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I63966007"],"apc_list":null,"apc_paid":null,"fwci":3.3394,"has_fulltext":false,"cited_by_count":55,"citation_normalized_percentile":{"value":0.9340535,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"81","issue":"18","first_page":"5714","last_page":"5718"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13187","display_name":"Diffusion and Search Dynamics","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/z-dna","display_name":"Z-DNA","score":0.9300000071525574},{"id":"https://openalex.org/keywords/supercoiled-dna","display_name":"supercoiled DNA","score":0.765999972820282},{"id":"https://openalex.org/keywords/bamhi","display_name":"BamHI","score":0.9670000076293945},{"id":"https://openalex.org/keywords/superhelical-density","display_name":"superhelical density","score":0.8659999966621399},{"id":"https://openalex.org/keywords/dna-structure","display_name":"DNA structure","score":0.36399999260902405}],"concepts":[{"id":"https://openalex.org/C42174275","wikidata":"https://www.wikidata.org/wiki/Q409191","display_name":"BamHI","level":4,"score":0.981611967086792},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.8220184445381165},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.7271616458892822},{"id":"https://openalex.org/C100594029","wikidata":"https://www.wikidata.org/wiki/Q7302658","display_name":"Recognition sequence","level":4,"score":0.6675261855125427},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.632912278175354},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5664141774177551},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5010690689086914},{"id":"https://openalex.org/C185581394","wikidata":"https://www.wikidata.org/wiki/Q145911","display_name":"Base pair","level":3,"score":0.49956178665161133},{"id":"https://openalex.org/C2780098074","wikidata":"https://www.wikidata.org/wiki/Q6037362","display_name":"Insert (composites)","level":2,"score":0.47210732102394104},{"id":"https://openalex.org/C158456008","wikidata":"https://www.wikidata.org/wiki/Q412639","display_name":"DNA supercoil","level":4,"score":0.46814945340156555},{"id":"https://openalex.org/C170673058","wikidata":"https://www.wikidata.org/wiki/Q780686","display_name":"Restriction map","level":4,"score":0.46534454822540283},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4057195782661438},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3232874870300293},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.30034780502319336},{"id":"https://openalex.org/C73573662","wikidata":"https://www.wikidata.org/wiki/Q130996","display_name":"DNA replication","level":3,"score":0.08308741450309753},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.07800343632698059},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0},{"id":"https://openalex.org/C43369102","wikidata":"https://www.wikidata.org/wiki/Q2307625","display_name":"Fracture (geology)","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004262","descriptor_name":"DNA Restriction Enzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004278","descriptor_name":"DNA, Superhelical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015245","descriptor_name":"Deoxyribonuclease BamHI","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015252","descriptor_name":"Deoxyribonucleases, Type II Site-Specific","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1073/pnas.81.18.5714","doi":"https://doi.org/10.1073/pnas.81.18.5714","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.81.18.5714","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:391781","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/391781","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmid:6091104","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6091104","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:391781","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/391781","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":20,"referenced_works":["https://openalex.org/W172591327","https://openalex.org/W1965060050","https://openalex.org/W1967174113","https://openalex.org/W1969241140","https://openalex.org/W1971481046","https://openalex.org/W1971670546","https://openalex.org/W1973503110","https://openalex.org/W1992595091","https://openalex.org/W1999669099","https://openalex.org/W2000075430","https://openalex.org/W2001818020","https://openalex.org/W2021436957","https://openalex.org/W2031926845","https://openalex.org/W2077667025","https://openalex.org/W2091489838","https://openalex.org/W2091506607","https://openalex.org/W2093262410","https://openalex.org/W2144241955","https://openalex.org/W2165855161","https://openalex.org/W2180006187"],"related_works":["https://openalex.org/W2022687072","https://openalex.org/W2187329380","https://openalex.org/W2021436957","https://openalex.org/W2015700711","https://openalex.org/W1972198820","https://openalex.org/W2413408313","https://openalex.org/W4390459516","https://openalex.org/W1606464636","https://openalex.org/W1606331919","https://openalex.org/W2130885824"],"abstract_inverted_index":{"Plasmids":[0],"containing":[1],"(C-G)n":[2,73],"inserts":[3],"have":[4],"been":[5],"used":[6],"to":[7,17,87,115],"study":[8],"the":[9,34,50,54,72,88,103,117,120,125],"inhibition":[10,55,99],"of":[11,56,64,69,119],"cleavage":[12,58,76],"by":[13,77],"restriction":[14],"endonucleases":[15],"due":[16],"Z-DNA":[18,70],"formation":[19],"in":[20,45,71],"negatively":[21],"supercoiled":[22],"plasmids.":[23],"The":[24,38],"enzyme":[25],"BssHII,":[26],"which":[27],"recognizes":[28],"G-C-G-C-G-C,":[29],"is":[30],"strongly":[31],"inhibited":[32,75],"when":[33,79,102],"insert":[35,74,89],"forms":[36],"Z-DNA.":[37],"BamHI":[39,57,78,104],"recognition":[40,81,105],"sequence":[41,82],"(G-G-A-T-C-C)":[42],"was":[43,59,83,100,107],"placed":[44],"four":[46,91],"different":[47],"positions":[48],"near":[49],"B-Z":[51,126],"junction":[52],"and":[53],"determined":[60],"as":[61],"a":[62],"function":[63],"negative":[65],"superhelical":[66],"density.":[67],"Formation":[68],"its":[80],"located":[84],"immediately":[85],"adjacent":[86],"or":[90],"base":[92,109],"pairs":[93,110],"away":[94],"from":[95],"it.":[96],"However,":[97],"no":[98],"found":[101],"site":[106],"eight":[108],"away.":[111],"These":[112],"experiments":[113],"help":[114],"define":[116],"limits":[118],"structural":[121],"perturbation":[122],"associated":[123],"with":[124],"junction.":[127]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}