{"id":"https://openalex.org/W2088069017","doi":"https://doi.org/10.1016/s0014-5793(98)00833-3","title":"Site directed mutagenesis reduces the Na+ affinity of HKT1, an Na+ energized high affinity K+ transporter","display_name":"Site directed mutagenesis reduces the Na+ affinity of HKT1, an Na+ energized high affinity K+ transporter","publication_year":1998,"publication_date":"1998-07-31","ids":{"openalex":"https://openalex.org/W2088069017","doi":"https://doi.org/10.1016/s0014-5793(98)00833-3","mag":"2088069017","pmid":"https://pubmed.ncbi.nlm.nih.gov/9710245"},"language":"en","primary_location":{"id":"doi:10.1016/s0014-5793(98)00833-3","doi":"https://doi.org/10.1016/s0014-5793(98)00833-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0014-5793(98)00833-3","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/S0014-5793%2898%2900833-3","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/S0014-5793%2898%2900833-3","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068512544","display_name":"Eugene Diatloff","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I5681781","display_name":"The University of Adelaide","ror":"https://ror.org/00892tw58","country_code":"AU","type":"education","lineage":["https://openalex.org/I4405259444","https://openalex.org/I5681781"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Eugene Diatloff","raw_affiliation_strings":["Department of Botany, University of Adelaide, SA, Australia","Department of Botany, University of Adelaide, Adelaide S.A. 5005. Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Botany, University of Adelaide, SA, Australia","institution_ids":["https://openalex.org/I5681781"]},{"raw_affiliation_string":"Department of Botany, University of Adelaide, Adelaide S.A. 5005. Australia","institution_ids":["https://openalex.org/I5681781"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078710789","display_name":"Raman Kumar","orcid":"https://orcid.org/0000-0003-4343-0951","observed_orcids":["https://orcid.org/0000-0003-4343-0951"]},"institutions":[{"id":"https://openalex.org/I5681781","display_name":"The University of Adelaide","ror":"https://ror.org/00892tw58","country_code":"AU","type":"education","lineage":["https://openalex.org/I4405259444","https://openalex.org/I5681781"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Raman Kumar","raw_affiliation_strings":["Department of Botany, University of Adelaide, Adelaide, S.A. 5005, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Botany, University of Adelaide, Adelaide, S.A. 5005, Australia","institution_ids":["https://openalex.org/I5681781"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028809050","display_name":"Daniel P. Schachtman","orcid":"https://orcid.org/0000-0003-1807-4369","observed_orcids":["https://orcid.org/0000-0003-1807-4369"]},"institutions":[{"id":"https://openalex.org/I5681781","display_name":"The University of Adelaide","ror":"https://ror.org/00892tw58","country_code":"AU","type":"education","lineage":["https://openalex.org/I4405259444","https://openalex.org/I5681781"]}],"countries":["AU"],"is_corresponding":true,"raw_author_name":"Daniel P Schachtman","raw_affiliation_strings":["Department of Botany, University of Adelaide, Adelaide, S.A. 5005, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Botany, University of Adelaide, Adelaide, S.A. 5005, Australia","institution_ids":["https://openalex.org/I5681781"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5028809050"],"corresponding_institution_ids":["https://openalex.org/I5681781"],"apc_list":{"value":3980,"currency":"USD","value_usd":3980},"apc_paid":null,"fwci":10.0608,"has_fulltext":true,"cited_by_count":57,"citation_normalized_percentile":{"value":0.98225034,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"432","issue":"1-2","first_page":"31","last_page":"36"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.8481000065803528,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.8481000065803528,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.09239999949932098,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":0.021900000050663948,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hkt1","display_name":"HKT1","score":0.9980000257492065},{"id":"https://openalex.org/keywords/na-k-transporters","display_name":"Na+/K+ transporters","score":0.7440000176429749},{"id":"https://openalex.org/keywords/sodium-affinity","display_name":"sodium affinity","score":0.6290000081062317},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.7799999713897705},{"id":"https://openalex.org/keywords/conserved-residues","display_name":"conserved residues","score":0.7919999957084656},{"id":"https://openalex.org/keywords/na-binding","display_name":"Na+ binding","score":0.5699999928474426},{"id":"https://openalex.org/keywords/potassium-transport","display_name":"potassium transport","score":0.6510000228881836},{"id":"https://openalex.org/keywords/wheat","display_name":"wheat","score":0.8040000200271606},{"id":"https://openalex.org/keywords/root-cortical-cells","display_name":"root cortical cells","score":0.8519999980926514},{"id":"https://openalex.org/keywords/yeast-expression-system","display_name":"yeast expression system","score":0.5080000162124634}],"concepts":[{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.6037351489067078},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.5725215673446655},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5114911794662476},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.511275053024292},{"id":"https://openalex.org/C149011108","wikidata":"https://www.wikidata.org/wiki/Q652985","display_name":"Transporter","level":3,"score":0.502868115901947},{"id":"https://openalex.org/C103408237","wikidata":"https://www.wikidata.org/wiki/Q2642668","display_name":"Site-directed mutagenesis","level":4,"score":0.4468761086463928},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4393855631351471},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.4349173307418823},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.42395079135894775},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36972880363464355},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.21428069472312927},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.15918433666229248}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001692","descriptor_name":"Biological Transport","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002414","descriptor_name":"Cations, Monovalent","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004252","descriptor_name":"DNA Mutational Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011188","descriptor_name":"Potassium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012964","descriptor_name":"Sodium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017124","descriptor_name":"Conserved Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D027682","descriptor_name":"Cation Transport Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D027981","descriptor_name":"Symporters","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0014-5793(98)00833-3","doi":"https://doi.org/10.1016/s0014-5793(98)00833-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0014-5793(98)00833-3","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/S0014-5793%2898%2900833-3","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},{"id":"pmid:9710245","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9710245","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS letters","raw_type":"Comparative Study"}],"best_oa_location":{"id":"doi:10.1016/s0014-5793(98)00833-3","doi":"https://doi.org/10.1016/s0014-5793(98)00833-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0014-5793(98)00833-3","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/S0014-5793%2898%2900833-3","source":{"id":"https://openalex.org/S104830714","display_name":"FEBS Letters","issn_l":"0014-5793","issn":["0014-5793","1873-3468"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEBS Letters","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2088069017.pdf","grobid_xml":"https://content.openalex.org/works/W2088069017.grobid-xml"},"referenced_works_count":30,"referenced_works":["https://openalex.org/W159884039","https://openalex.org/W1536270671","https://openalex.org/W1604517711","https://openalex.org/W1685356864","https://openalex.org/W1939241926","https://openalex.org/W1954232128","https://openalex.org/W1964583472","https://openalex.org/W1972180775","https://openalex.org/W1979667526","https://openalex.org/W1987475842","https://openalex.org/W2016026343","https://openalex.org/W2021199264","https://openalex.org/W2028143827","https://openalex.org/W2035624409","https://openalex.org/W2037944731","https://openalex.org/W2044540096","https://openalex.org/W2046200067","https://openalex.org/W2047219099","https://openalex.org/W2055043387","https://openalex.org/W2060913542","https://openalex.org/W2075257287","https://openalex.org/W2078420488","https://openalex.org/W2089606003","https://openalex.org/W2091324720","https://openalex.org/W2094832359","https://openalex.org/W2109288052","https://openalex.org/W2118056423","https://openalex.org/W2120684043","https://openalex.org/W2140014285","https://openalex.org/W2165795960"],"related_works":["https://openalex.org/W2109811688","https://openalex.org/W2375700481","https://openalex.org/W1526783183","https://openalex.org/W3094559755","https://openalex.org/W2105345446","https://openalex.org/W2046200067","https://openalex.org/W2037944731","https://openalex.org/W2383308070","https://openalex.org/W2157032799","https://openalex.org/W2983921726"],"abstract_inverted_index":{"HKT1":[0,41,94,118,181],"encodes":[1],"a":[2,38,51,64,183],"high":[3],"affinity":[4,116,126,143],"Na+":[5,31,120],"coupled":[6],"K+":[7,55],"transporter":[8,146],"expressed":[9],"in":[10,25,132,180,185],"the":[11,22,26,68,72,89,96,101,111,115,133,142,145,161,186],"cortical":[12],"cells":[13],"of":[14,21,30,40,53,71,81,91,103,117,144,151,172,190],"Triticum":[15],"aestivum":[16],"roots.":[17],"To":[18],"identify":[19],"regions":[20],"protein":[23],"involved":[24],"binding":[27,187],"and":[28,32,188],"transport":[29,56,189],"K+,":[33],"mutations":[34,62],"were":[35],"introduced":[36],"into":[37],"domain":[39,179],"containing":[42,95],"16":[43],"amino":[44,155],"acids":[45],"that":[46,79,110,139,163,177],"are":[47],"highly":[48,153],"conserved":[49,154,167],"across":[50],"range":[52],"putative":[54],"proteins":[57],"from":[58],"different":[59],"phyla.":[60],"Two":[61],"had":[63],"significant":[65],"effect":[66],"on":[67],"functional":[69],"characteristics":[70],"transporter.":[73],"A":[74,129],"yeast":[75,92,104],"growth":[76,90,102],"assay":[77],"showed":[78],"concentrations":[80],"NaCl":[82],"between":[83],"2.5":[84],"to":[85],"50":[86],"mM":[87],"stimulated":[88],"expressing":[93,105],"E464Q":[97,112],"substitution,":[98],"but":[99,121],"not":[100,123],"HKT1.":[106],"Kinetic":[107],"analysis":[108,175],"confirmed":[109],"mutation":[113,131],"lowered":[114,141],"for":[119,127,147],"did":[122],"affect":[124],"its":[125],"K+.":[128],"second":[130],"same":[134],"region":[135],"F463L":[136],"was":[137],"created":[138],"also":[140],"Na+.":[148,191],"The":[149,170],"importance":[150],"these":[152],"acid":[156],"residues":[157],"is":[158],"highlighted":[159],"by":[160],"fact":[162],"they":[164],"have":[165],"remained":[166],"through":[168],"evolution.":[169],"results":[171],"this":[173,178],"mutational":[174],"suggest":[176],"plays":[182],"role":[184]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}