{"id":"https://openalex.org/W2087544933","doi":"https://doi.org/10.1371/journal.pone.0043093","title":"PCR Biases Distort Bacterial and Archaeal Community Structure in Pyrosequencing Datasets","display_name":"PCR Biases Distort Bacterial and Archaeal Community Structure in Pyrosequencing Datasets","publication_year":2012,"publication_date":"2012-08-15","ids":{"openalex":"https://openalex.org/W2087544933","doi":"https://doi.org/10.1371/journal.pone.0043093","mag":"2087544933","pmid":"https://pubmed.ncbi.nlm.nih.gov/22905208","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3419673"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0043093","doi":"https://doi.org/10.1371/journal.pone.0043093","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0043093","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0043093&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0043093&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5001255806","display_name":"Ameet J. Pinto","orcid":"https://orcid.org/0000-0003-1089-5664","observed_orcids":["https://orcid.org/0000-0003-1089-5664"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ameet J. Pinto","raw_affiliation_strings":["Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, United States of America","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5019834116","display_name":"Lutgarde M. Raskin","orcid":"https://orcid.org/0000-0002-9625-4034","observed_orcids":["https://orcid.org/0000-0002-9625-4034"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Lutgarde Raskin","raw_affiliation_strings":["Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, United States of America","institution_ids":["https://openalex.org/I27837315"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5019834116"],"corresponding_institution_ids":["https://openalex.org/I27837315"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":32.3442,"has_fulltext":true,"cited_by_count":460,"citation_normalized_percentile":{"value":0.99923878,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":97,"max":100},"biblio":{"volume":"7","issue":"8","first_page":"e43093","last_page":"e43093"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.8227999806404114,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.8227999806404114,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.14319999516010284,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.01640000008046627,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pcr-bias","display_name":"PCR bias","score":0.9300000071525574},{"id":"https://openalex.org/keywords/16s-rrna-gene-sequencing","display_name":"16S rRNA gene sequencing","score":0.8309999704360962},{"id":"https://openalex.org/keywords/microbial-community-structure","display_name":"microbial community structure","score":0.734000027179718},{"id":"https://openalex.org/keywords/mock-communities","display_name":"mock communities","score":0.6349999904632568},{"id":"https://openalex.org/keywords/sequencing-depth","display_name":"sequencing depth","score":0.6389999985694885},{"id":"https://openalex.org/keywords/sequencing-errors","display_name":"sequencing errors","score":0.9729999899864197},{"id":"https://openalex.org/keywords/otu-richness","display_name":"OTU richness","score":0.6150000095367432},{"id":"https://openalex.org/keywords/beta-diversity","display_name":"beta diversity","score":0.6039999723434448},{"id":"https://openalex.org/keywords/alpha-diversity","display_name":"alpha diversity","score":0.7459999918937683},{"id":"https://openalex.org/keywords/archaea","display_name":"archaea","score":0.5619999766349792},{"id":"https://openalex.org/keywords/bacteria","display_name":"bacteria","score":0.9520000219345093},{"id":"https://openalex.org/keywords/primer-design","display_name":"primer design","score":0.6589999794960022},{"id":"https://openalex.org/keywords/relative-abundance-estimation","display_name":"relative abundance estimation","score":0.6069999933242798}],"concepts":[{"id":"https://openalex.org/C125009961","wikidata":"https://www.wikidata.org/wiki/Q2342248","display_name":"Pyrosequencing","level":3,"score":0.8549191951751709},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7990787625312805},{"id":"https://openalex.org/C53565203","wikidata":"https://www.wikidata.org/wiki/Q17146659","display_name":"Species richness","level":2,"score":0.6768441796302795},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6496836543083191},{"id":"https://openalex.org/C15151743","wikidata":"https://www.wikidata.org/wiki/Q903778","display_name":"Metagenomics","level":3,"score":0.5806174874305725},{"id":"https://openalex.org/C192800701","wikidata":"https://www.wikidata.org/wiki/Q6784807","display_name":"Massive parallel sequencing","level":4,"score":0.5747603178024292},{"id":"https://openalex.org/C42062724","wikidata":"https://www.wikidata.org/wiki/Q1209205","display_name":"16S ribosomal RNA","level":3,"score":0.5294413566589355},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.528901994228363},{"id":"https://openalex.org/C2781162219","wikidata":"https://www.wikidata.org/wiki/Q26250693","display_name":"Replicate","level":2,"score":0.5201659202575684},{"id":"https://openalex.org/C164928082","wikidata":"https://www.wikidata.org/wiki/Q6063435","display_name":"Ion semiconductor sequencing","level":4,"score":0.5192882418632507},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.5021011829376221},{"id":"https://openalex.org/C133079900","wikidata":"https://www.wikidata.org/wiki/Q5155065","display_name":"Community structure","level":2,"score":0.46311914920806885},{"id":"https://openalex.org/C132917006","wikidata":"https://www.wikidata.org/wiki/Q5250436","display_name":"Deep sequencing","level":4,"score":0.44642823934555054},{"id":"https://openalex.org/C97256817","wikidata":"https://www.wikidata.org/wiki/Q1462316","display_name":"Spurious relationship","level":2,"score":0.4413600265979767},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.38510680198669434},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.35042423009872437},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.1566406786441803},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.11869186162948608},{"id":"https://openalex.org/C119857082","wikidata":"https://www.wikidata.org/wiki/Q2539","display_name":"Machine learning","level":1,"score":0.11060747504234314},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.1037270724773407},{"id":"https://openalex.org/C105795698","wikidata":"https://www.wikidata.org/wiki/Q12483","display_name":"Statistics","level":1,"score":0.09344315528869629},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D001105","descriptor_name":"Archaea","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005821","descriptor_name":"Genetic Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D019766","descriptor_name":"Genes, Archaeal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D030541","descriptor_name":"Databases, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":6,"locations":[{"id":"doi:10.1371/journal.pone.0043093","doi":"https://doi.org/10.1371/journal.pone.0043093","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0043093","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0043093&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},{"id":"pmid:22905208","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22905208","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:3419673","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC3419673/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmh:oai:eprints.gla.ac.uk:68685","doi":null,"is_oa":false,"landing_page_url":"https://eprints.gla.ac.uk/68685/1/68685.pdf","pdf_url":"https://eprints.gla.ac.uk/68685/1/68685.pdf","source":{"id":"https://openalex.org/S4210235606","display_name":"ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam)","issn_l":"2622-8912","issn":["2622-8912","2622-8920"],"is_oa":false,"is_in_doaj":true,"is_core":false,"listed_in":["doaj"],"host_organization":"https://openalex.org/I4405259220","host_organization_name":"Institut Agama Islam Negeri Langsa","host_organization_lineage":["https://openalex.org/I4405259220"],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Articles"},{"id":"pmh:oai:doaj.org/article:c9f78ca3d50f4bb8b99534ed48f840e4","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/c9f78ca3d50f4bb8b99534ed48f840e4","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 7, Iss 8, p e43093 (2012)","raw_type":"article"},{"id":"pmh:oai:figshare.com:article/121470","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/dataset/PCR_Biases_Distort_Bacterial_and_Archaeal_Community_Structure_in_Pyrosequencing_Datasets/121470","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dataset"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0043093","doi":"https://doi.org/10.1371/journal.pone.0043093","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0043093","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0043093&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2493301281","display_name":"BE/GEN-EN:     Elucidating Mechanisms Responsible for the Persistence of Microorganisms in Drinking Water Distribution Systems via DNA Microarray Technology","funder_award_id":"0412618","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G6579759139","display_name":"Low-temperature Anaerobic Membrane Bioreactors for Sustainable Domestic Wastewater Treatment","funder_award_id":"1133793","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G799429350","display_name":"Biologically-mediated, simultaneous removal of nitrate and arsenic from drinking water sources","funder_award_id":"0967707","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320337390","display_name":"Division of Chemical, Bioengineering, Environmental, and Transport Systems","ror":"https://ror.org/0471zv972"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2087544933.pdf","grobid_xml":"https://content.openalex.org/works/W2087544933.grobid-xml"},"referenced_works_count":67,"referenced_works":["https://openalex.org/W346827628","https://openalex.org/W1512323415","https://openalex.org/W1546639740","https://openalex.org/W1561580658","https://openalex.org/W1582334595","https://openalex.org/W1964805664","https://openalex.org/W1965866182","https://openalex.org/W1967418136","https://openalex.org/W1969435169","https://openalex.org/W1971536609","https://openalex.org/W1974265848","https://openalex.org/W1979343540","https://openalex.org/W1991486768","https://openalex.org/W2000072710","https://openalex.org/W2012319221","https://openalex.org/W2032525833","https://openalex.org/W2036647435","https://openalex.org/W2040986336","https://openalex.org/W2042071496","https://openalex.org/W2056857250","https://openalex.org/W2059237622","https://openalex.org/W2060254952","https://openalex.org/W2066988911","https://openalex.org/W2067297267","https://openalex.org/W2072556768","https://openalex.org/W2074003662","https://openalex.org/W2080283023","https://openalex.org/W2081411589","https://openalex.org/W2087671769","https://openalex.org/W2091922579","https://openalex.org/W2094828133","https://openalex.org/W2101120467","https://openalex.org/W2103412200","https://openalex.org/W2105522288","https://openalex.org/W2106308757","https://openalex.org/W2108718991","https://openalex.org/W2109167494","https://openalex.org/W2114766231","https://openalex.org/W2114859967","https://openalex.org/W2115639950","https://openalex.org/W2118526609","https://openalex.org/W2120821495","https://openalex.org/W2125439893","https://openalex.org/W2127862315","https://openalex.org/W2130077037","https://openalex.org/W2131088968","https://openalex.org/W2136879569","https://openalex.org/W2140319224","https://openalex.org/W2142686163","https://openalex.org/W2145166062","https://openalex.org/W2147199223","https://openalex.org/W2150914285","https://openalex.org/W2151497278","https://openalex.org/W2155066423","https://openalex.org/W2156465688","https://openalex.org/W2157032655","https://openalex.org/W2157450307","https://openalex.org/W2159748283","https://openalex.org/W2162578061","https://openalex.org/W2165065263","https://openalex.org/W2166171121","https://openalex.org/W2168104108","https://openalex.org/W2913345319","https://openalex.org/W3145257564","https://openalex.org/W3199943451","https://openalex.org/W4239131029","https://openalex.org/W4254928341"],"related_works":["https://openalex.org/W2103923544","https://openalex.org/W1656962512","https://openalex.org/W2032502193","https://openalex.org/W7095733718","https://openalex.org/W2885695352","https://openalex.org/W2013029861","https://openalex.org/W2103412200","https://openalex.org/W2519703804","https://openalex.org/W2153092015","https://openalex.org/W1972304471"],"abstract_inverted_index":{"As":[0,154],"16S":[1,75,173],"rRNA":[2,76,174],"gene":[3,77],"targeted":[4],"massively":[5],"parallel":[6],"sequencing":[7,27,89,92,183,198,225,232],"has":[8,39],"become":[9],"a":[10,235],"common":[11],"tool":[12],"for":[13],"microbial":[14,52],"diversity":[15],"investigations,":[16],"numerous":[17],"advances":[18],"have":[19,234],"been":[20,40],"made":[21],"to":[22,83,180,289],"minimize":[23],"the":[24,45,85,107,118,134,147,168,172,188,194,215,221,268,277],"influence":[25,119],"of":[26,47,87,109,120,122,136,150,156,171,184,196,223,259,284],"and":[28,62,72,96,100,128,140,146,162,205,244,256],"chimeric":[29,97],"PCR":[30,49,98,185,294],"artifacts":[31,93],"through":[32],"rigorous":[33],"quality":[34],"control":[35],"measures.":[36],"However,":[37,267],"there":[38],"little":[41],"effort":[42],"towards":[43,106,271],"understanding":[44],"effect":[46],"multi-template":[48,104,293],"biases":[50,102,290],"on":[51,126,133,238],"community":[53,110,251,274],"structure.":[54],"In":[55],"this":[56,157],"study,":[57,158],"we":[58,159],"used":[59],"three":[60,63,124],"bacterial":[61,71,161],"archaeal":[64,74,163],"mock":[65],"communities":[66],"consisting":[67],"of,":[68],"respectively,":[69],"33":[70],"24":[73],"sequences":[78],"combined":[79],"in":[80,103,112,207,210,279],"different":[81],"proportions":[82],"compare":[84],"influences":[86],"(1)":[88],"depth,":[90],"(2)":[91],"(sequencing":[94],"errors":[95,233,278],"artifacts),":[99],"(3)":[101],"PCR,":[105],"interpretation":[108],"structure":[111,252,275],"pyrosequencing":[113],"datasets.":[114],"We":[115,191],"also":[116],"assessed":[117],"each":[121,285],"these":[123],"variables":[125],"\u03b1-":[127,243],"\u03b2-diversity":[129,211,245],"metrics":[130,248],"that":[131,142,166,193,249],"rely":[132],"number":[135],"OTUs":[137,152,229],"alone":[138],"(richness)":[139],"those":[141],"include":[143],"both":[144,254],"membership":[145],"relative":[148,257,282],"abundance":[149,258,283],"detected":[151,286],"(diversity).":[153],"part":[155],"redesigned":[160],"primer":[164],"sets":[165],"target":[167],"V3-V5":[169],"region":[170],"gene,":[175],"along":[176],"with":[177,292],"multiplexing":[178],"barcodes,":[179],"permit":[181],"simultaneous":[182],"products":[186],"from":[187,231],"two":[189],"domains.":[190],"conclude":[192],"benefits":[195],"deeper":[197],"efforts":[199],"extend":[200],"beyond":[201],"greater":[202],"OTU":[203,287],"detection":[204],"result":[206],"higher":[208],"precision":[209],"analyses":[212],"by":[213,264],"reducing":[214],"variability":[216],"between":[217],"replicate":[218],"libraries,":[219],"despite":[220],"presence":[222],"more":[224],"artifacts.":[226],"Additionally,":[227],"spurious":[228,265],"resulting":[230],"significant":[236],"impact":[237],"richness":[239,255],"or":[240],"shared-richness":[241],"based":[242],"metrics,":[246],"whereas":[247],"utilize":[250],"(including":[253],"OTUs)":[260],"are":[261,276],"minimally":[262],"affected":[263],"OTUs.":[266],"greatest":[269],"obstacle":[270],"accurately":[272],"evaluating":[273],"estimated":[280],"mean":[281],"due":[288],"associated":[291],"reactions.":[295]},"counts_by_year":[{"year":2026,"cited_by_count":12},{"year":2025,"cited_by_count":20},{"year":2024,"cited_by_count":23},{"year":2023,"cited_by_count":18},{"year":2022,"cited_by_count":33},{"year":2021,"cited_by_count":30},{"year":2020,"cited_by_count":24},{"year":2019,"cited_by_count":24},{"year":2018,"cited_by_count":43},{"year":2017,"cited_by_count":43},{"year":2016,"cited_by_count":59},{"year":2015,"cited_by_count":54},{"year":2014,"cited_by_count":42},{"year":2013,"cited_by_count":29},{"year":2012,"cited_by_count":5}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}
