{"id":"https://openalex.org/W2087541706","doi":"https://doi.org/10.1002/(sici)1520-6327(1996)31:4<451::aid-arch7>3.0.co;2-y","title":"Response of digestive cysteine proteinases from the colorado potato beetle (Leptinotarsa decemlineata) and the black vine weevil (Otiorynchus sulcatus) to a recombinant form of human stefin A","display_name":"Response of digestive cysteine proteinases from the colorado potato beetle (Leptinotarsa decemlineata) and the black vine weevil (Otiorynchus sulcatus) to a recombinant form of human stefin A","publication_year":1996,"publication_date":"1996-01-01","ids":{"openalex":"https://openalex.org/W2087541706","doi":"https://doi.org/10.1002/(sici)1520-6327(1996)31:4<451::aid-arch7>3.0.co;2-y","mag":"2087541706","pmid":"https://pubmed.ncbi.nlm.nih.gov/8920105"},"language":"en","primary_location":{"id":"doi:10.1002/(sici)1520-6327(1996)31:4<451::aid-arch7>3.0.co;2-y","doi":"https://doi.org/10.1002/(sici)1520-6327(1996)31:4<451::aid-arch7>3.0.co;2-y","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1520-6327(1996)31:4<451::aid-arch7>3.0.co;2-y","pdf_url":null,"source":{"id":"https://openalex.org/S14369749","display_name":"Archives of Insect Biochemistry and Physiology","issn_l":"0739-4462","issn":["0739-4462","1520-6327"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","medline","norway-1","poland-40","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of Insect Biochemistry and Physiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5045176518","display_name":"Dominique Michaud","orcid":"https://orcid.org/0000-0002-5177-1142","observed_orcids":["https://orcid.org/0000-0002-5177-1142","https://orcid.org/0000-0002-6018-9023"]},"institutions":[{"id":"https://openalex.org/I43406934","display_name":"Universit\u00e9 Laval","ror":"https://ror.org/04sjchr03","country_code":"CA","type":"education","lineage":["https://openalex.org/I43406934"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"Dominique Michaud","raw_affiliation_strings":["D\u00e9partement de phytologie, Universit\u00e9 Laval, Qu\u00e9bec, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"D\u00e9partement de phytologie, Universit\u00e9 Laval, Qu\u00e9bec, Canada","institution_ids":["https://openalex.org/I43406934"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110149348","display_name":"Binh Nguyen\u2010Quoc","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I43406934","display_name":"Universit\u00e9 Laval","ror":"https://ror.org/04sjchr03","country_code":"CA","type":"education","lineage":["https://openalex.org/I43406934"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Binh Nguyen-Quoc","raw_affiliation_strings":["Centre de Recherche en Horticulture, De\u0301partement de phytologie, Faculte\u0301 des Sciences de l'Agriculture et de l'Alimentation, Universite\u0301 Laval, Que\u0301bec","Centre de Recherche en Horticulture, D\u00e9partement de phytologie, Facult\u00e9 des Sciences de l'Agriculture et de l'Alimentation, Universit\u00e9 Laval, Qu\u00e9bec"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre de Recherche en Horticulture, De\u0301partement de phytologie, Faculte\u0301 des Sciences de l'Agriculture et de l'Alimentation, Universite\u0301 Laval, Que\u0301bec","institution_ids":["https://openalex.org/I43406934"]},{"raw_affiliation_string":"Centre de Recherche en Horticulture, D\u00e9partement de phytologie, Facult\u00e9 des Sciences de l'Agriculture et de l'Alimentation, Universit\u00e9 Laval, Qu\u00e9bec","institution_ids":["https://openalex.org/I43406934"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042065932","display_name":"Thierry C. Vrain","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1331897569","display_name":"Agriculture and Agri-Food Canada","ror":"https://ror.org/051dzs374","country_code":"CA","type":"government","lineage":["https://openalex.org/I1331897569","https://openalex.org/I2802286613"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Thierry C. Vrain","raw_affiliation_strings":["Pacific Agriculture Research Centre, Agriculture and Agri-Food Canada, Vancouver, B. C., Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pacific Agriculture Research Centre, Agriculture and Agri-Food Canada, Vancouver, B. C., Canada","institution_ids":["https://openalex.org/I1331897569"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012539033","display_name":"Dunne Fong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I102322142","display_name":"Rutgers, The State University of New Jersey","ror":"https://ror.org/05vt9qd57","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dunne Fong","raw_affiliation_strings":["Department of Biological Sciences, Rutgers, The State University of New Jersey, Piscataway, New Jersey"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Rutgers, The State University of New Jersey, Piscataway, New Jersey","institution_ids":["https://openalex.org/I102322142"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112254967","display_name":"Serge Yelle","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I43406934","display_name":"Universit\u00e9 Laval","ror":"https://ror.org/04sjchr03","country_code":"CA","type":"education","lineage":["https://openalex.org/I43406934"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Serge Yelle","raw_affiliation_strings":["Centre de Recherche en Horticulture, De\u0301partement de phytologie, Faculte\u0301 des Sciences de l'Agriculture et de l'Alimentation, Universite\u0301 Laval, Que\u0301bec","Centre de Recherche en Horticulture, D\u00e9partement de phytologie, Facult\u00e9 des Sciences de l'Agriculture et de l'Alimentation, Universit\u00e9 Laval, Qu\u00e9bec"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre de Recherche en Horticulture, De\u0301partement de phytologie, Faculte\u0301 des Sciences de l'Agriculture et de l'Alimentation, Universite\u0301 Laval, Que\u0301bec","institution_ids":["https://openalex.org/I43406934"]},{"raw_affiliation_string":"Centre de Recherche en Horticulture, D\u00e9partement de phytologie, Facult\u00e9 des Sciences de l'Agriculture et de l'Alimentation, Universit\u00e9 Laval, Qu\u00e9bec","institution_ids":["https://openalex.org/I43406934"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5045176518"],"corresponding_institution_ids":["https://openalex.org/I43406934"],"apc_list":{"value":3920,"currency":"USD","value_usd":3920},"apc_paid":null,"fwci":2.8903,"has_fulltext":false,"cited_by_count":48,"citation_normalized_percentile":{"value":0.92492231,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"31","issue":"4","first_page":"451","last_page":"464"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.538100004196167,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.538100004196167,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11075","display_name":"Insect Resistance and Genetics","score":0.38109999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11065","display_name":"Insect Pest Control Strategies","score":0.02410000003874302,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cysteine-protease","display_name":"cysteine protease","score":0.7720000147819519},{"id":"https://openalex.org/keywords/stefin-a","display_name":"stefin A","score":0.7979999780654907},{"id":"https://openalex.org/keywords/oryzacystatin-i","display_name":"oryzacystatin I","score":0.9810000061988831},{"id":"https://openalex.org/keywords/colorado-potato-beetle","display_name":"Colorado potato beetle","score":0.9070000052452087},{"id":"https://openalex.org/keywords/black-vine-weevil","display_name":"black vine weevil","score":0.8870000243186951},{"id":"https://openalex.org/keywords/protease-inhibition","display_name":"protease inhibition","score":0.4000000059604645},{"id":"https://openalex.org/keywords/coleopteran-pests","display_name":"coleopteran pests","score":0.8289999961853027},{"id":"https://openalex.org/keywords/midgut","display_name":"midgut","score":0.7720000147819519}],"concepts":[{"id":"https://openalex.org/C2777266796","wikidata":"https://www.wikidata.org/wiki/Q156954","display_name":"Colorado potato beetle","level":3,"score":0.8802504539489746},{"id":"https://openalex.org/C2779707326","wikidata":"https://www.wikidata.org/wiki/Q602990","display_name":"Leptinotarsa","level":3,"score":0.8683424592018127},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8343925476074219},{"id":"https://openalex.org/C2781214258","wikidata":"https://www.wikidata.org/wiki/Q917284","display_name":"Vine","level":2,"score":0.783193826675415},{"id":"https://openalex.org/C2780817911","wikidata":"https://www.wikidata.org/wiki/Q383963","display_name":"Weevil","level":2,"score":0.5798351764678955},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5350708961486816},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.49282458424568176},{"id":"https://openalex.org/C22508944","wikidata":"https://www.wikidata.org/wiki/Q568174","display_name":"PEST analysis","level":2,"score":0.355180561542511},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18197649717330933},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12515002489089966}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001517","descriptor_name":"Coleoptera","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D003546","descriptor_name":"Cysteine Endopeptidases","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D003546","descriptor_name":"Cysteine Endopeptidases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004064","descriptor_name":"Digestive System","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015853","descriptor_name":"Cysteine Proteinase Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D015891","descriptor_name":"Cystatins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D015891","descriptor_name":"Cystatins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055312","descriptor_name":"Cystatin A","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/(sici)1520-6327(1996)31:4<451::aid-arch7>3.0.co;2-y","doi":"https://doi.org/10.1002/(sici)1520-6327(1996)31:4<451::aid-arch7>3.0.co;2-y","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1520-6327(1996)31:4<451::aid-arch7>3.0.co;2-y","pdf_url":null,"source":{"id":"https://openalex.org/S14369749","display_name":"Archives of Insect Biochemistry and Physiology","issn_l":"0739-4462","issn":["0739-4462","1520-6327"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","medline","norway-1","poland-40","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of Insect Biochemistry and Physiology","raw_type":"journal-article"},{"id":"pmid:8920105","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8920105","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of insect biochemistry and physiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Zero hunger","score":0.4359000027179718,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W560734277","https://openalex.org/W1515058107","https://openalex.org/W1586931683","https://openalex.org/W1965428226","https://openalex.org/W1982031046","https://openalex.org/W1982746491","https://openalex.org/W1985665984","https://openalex.org/W1989171704","https://openalex.org/W1996223704","https://openalex.org/W2002753601","https://openalex.org/W2011531981","https://openalex.org/W2024959455","https://openalex.org/W2029963258","https://openalex.org/W2036097122","https://openalex.org/W2044095446","https://openalex.org/W2056818090","https://openalex.org/W2063631001","https://openalex.org/W2068446924","https://openalex.org/W2072142894","https://openalex.org/W2082089614","https://openalex.org/W2088550607","https://openalex.org/W2088819981","https://openalex.org/W2100837269","https://openalex.org/W2128635872","https://openalex.org/W2160405746","https://openalex.org/W2413304998","https://openalex.org/W2492361169","https://openalex.org/W2894910962","https://openalex.org/W4238776363"],"related_works":["https://openalex.org/W2002753601","https://openalex.org/W2047543496","https://openalex.org/W2000319100","https://openalex.org/W2413304998","https://openalex.org/W2594340397","https://openalex.org/W2075107586","https://openalex.org/W1989171704","https://openalex.org/W1967768294","https://openalex.org/W2057195859","https://openalex.org/W2565708833"],"abstract_inverted_index":{"The":[0],"effects":[1,151],"of":[2,17,44,67,101,113,152,161,180,187,196,206,240,247],"the":[3,18,26,45,76,83,102,110,123,130,135,150,153,158,162,178,185,194,197,219,238,245],"cystatins,":[4],"human":[5],"stefin":[6],"A":[7],"(HSA)":[8],"and":[9,25,41,53,64,140],"oryzacystatin":[10],"I":[11],"(OCI)":[12],"on":[13,157,200],"digestive":[14],"cysteine":[15,116,222],"proteinases":[16,137,223],"Colorado":[19],"potato":[20],"beetle":[21],"(CPB),":[22],"Leptinotarsa":[23],"decemlineata,":[24],"black":[27],"vine":[28],"weevil":[29],"(BVW),":[30],"Otiorynchus":[31],"sulcatus,":[32],"were":[33,79,165],"assessed":[34],"using":[35,144],"complementary":[36,95],"inhibition":[37,66,96,100],"assays,":[38,97],"cystatin-affinity":[39],"chromatography,":[40,149],"recombinant":[42],"forms":[43],"two":[46,114,163],"inhibitors.":[47],"For":[48],"both":[49],"insects,":[50],"either":[51,120],"HSA":[52,106,172,207],"OCI":[54,75,168],"used":[55,86,226],"in":[56,87,119,227],"excess":[57,89],"(10":[58],"or":[59],"20":[60],"microM)":[61],"caused":[62],"partial":[63],"stable":[65,182],"total":[68],"proteolytic":[69,209],"(azocaseinase)":[70],"activity,":[71],"but":[72],"unlike":[73],"for":[74,244],"HSA-mediated":[77],"inhibitions":[78],"significantly":[80],"increased":[81],"when":[82,224,236],"inhibitor":[84],"was":[85,107,173],"large":[88,228],"(100":[90],"microM).":[91],"As":[92],"demonstrated":[93],"by":[94,105],"this":[98,201],"two-step":[99],"insect":[103,121,154,198,220],"proteases":[104,156,199],"due":[108],"to":[109,129,175,193,208,217],"differential":[111],"inactivation":[112],"distinct":[115],"proteinase":[117],"populations":[118],"extracts,":[122],"rapidly":[124],"(strongly)":[125],"inhibited":[126],"population":[127],"corresponding":[128],"OCI-sensitive":[131],"fraction.":[132],"After":[133],"removing":[134],"cystatin-sensitive":[136],"from":[138],"CPB":[139],"BVW":[141],"midgut":[142],"extracts":[143],"OCI-":[145],"(or":[146],"HSA-)":[147],"affinity":[148],"\"non-target\"":[155],"structural":[159],"integrity":[160],"cystatins":[164],"assessed.":[166],"While":[167],"remained":[169],"essentially":[170],"stable,":[171],"subjected":[174],"hydrolysis":[176],"without":[177],"accumulation":[179],"detectable":[181],"intermediates,":[183],"suggesting":[184],"presence":[186],"multiple":[188],"exposed":[189],"cleavage":[190,210],"sites":[191],"sensitive":[192],"action":[195],"cystatin.":[202],"This":[203],"apparent":[204],"susceptibility":[205],"may":[211],"partially":[212],"explain":[213],"its":[214],"low":[215],"efficiency":[216],"inactivate":[218],"OCI-insensitive":[221],"not":[225],"excess.":[229],"It":[230],"could":[231],"also":[232],"have":[233],"major":[234],"implications":[235],"planning":[237],"use":[239],"cystatin-expressing":[241],"transgenic":[242],"plants":[243],"control":[246],"coleopteran":[248],"pests.":[249]},"counts_by_year":[{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}