{"id":"https://openalex.org/W2087257548","doi":"https://doi.org/10.1002/bit.260390805","title":"Affinity purification of insoluble recombinant fusion proteins containing glutathione\u2010S\u2010transferase","display_name":"Affinity purification of insoluble recombinant fusion proteins containing glutathione\u2010S\u2010transferase","publication_year":1992,"publication_date":"1992-04-05","ids":{"openalex":"https://openalex.org/W2087257548","doi":"https://doi.org/10.1002/bit.260390805","mag":"2087257548","pmid":"https://pubmed.ncbi.nlm.nih.gov/18601017"},"language":"en","primary_location":{"id":"doi:10.1002/bit.260390805","doi":"https://doi.org/10.1002/bit.260390805","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.260390805","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029717334","display_name":"John L. Hartman","orcid":"https://orcid.org/0000-0003-1176-6469","observed_orcids":["https://orcid.org/0000-0003-1176-6469"]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]},{"id":"https://openalex.org/I4390039250","display_name":"UAB Medicine","ror":"https://ror.org/036554539","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039250"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John Hartman","raw_affiliation_strings":["Departments of Medicine, Division of Hematology and Oncology, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005, USA","Departments of Medicine, Division of Hematology and Oncology, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005","School of Medicine, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Medicine, Division of Hematology and Oncology, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005, USA","institution_ids":["https://openalex.org/I32389192","https://openalex.org/I4390039250"]},{"raw_affiliation_string":"Departments of Medicine, Division of Hematology and Oncology, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005","institution_ids":["https://openalex.org/I32389192"]},{"raw_affiliation_string":"School of Medicine, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031776003","display_name":"Paru Daram","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paru Daram","raw_affiliation_strings":["Departments of Medicine, Division of Hematology and Oncology, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Medicine, Division of Hematology and Oncology, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026190252","display_name":"Raymond A. Frizzell","orcid":"https://orcid.org/0000-0003-0167-4421","observed_orcids":["https://orcid.org/0000-0003-0167-4421"]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Raymond A. Frizzell","raw_affiliation_strings":["Departments of Physiology and Biophysics, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Physiology and Biophysics, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028512124","display_name":"Thomas A. Rado","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas Rado","raw_affiliation_strings":["Departments of Medicine, Division of Hematology and Oncology, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Medicine, Division of Hematology and Oncology, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008621245","display_name":"Dale John Benos","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dale J. Benos","raw_affiliation_strings":["Departments of Physiology and Biophysics, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Physiology and Biophysics, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5050704941","display_name":"Eric J. Sorscher","orcid":"https://orcid.org/0000-0001-9341-3354","observed_orcids":["https://orcid.org/0000-0001-9341-3354"]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Eric J. Sorscher","raw_affiliation_strings":["Departments of Medicine, Division of Hematology and Oncology, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005","Departments of Physiology and Biophysics, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Medicine, Division of Hematology and Oncology, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005","institution_ids":["https://openalex.org/I32389192"]},{"raw_affiliation_string":"Departments of Physiology and Biophysics, UAB Station, University of Alabama at Birmingham, Birmingham, AL 35294-0005","institution_ids":["https://openalex.org/I32389192"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5050704941"],"corresponding_institution_ids":["https://openalex.org/I32389192"],"apc_list":{"value":5060,"currency":"USD","value_usd":5060},"apc_paid":null,"fwci":0.9165,"has_fulltext":false,"cited_by_count":21,"citation_normalized_percentile":{"value":0.73087725,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"39","issue":"8","first_page":"828","last_page":"832"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glutathione-s-transferase","display_name":"glutathione S-transferase","score":0.8709999918937683},{"id":"https://openalex.org/keywords/recombinant-fusion-protein","display_name":"recombinant fusion protein","score":0.9039999842643738},{"id":"https://openalex.org/keywords/protein-solubility","display_name":"protein solubility","score":0.7149999737739563},{"id":"https://openalex.org/keywords/cystic-fibrosis-transmembrane-conductance-regulator","display_name":"cystic fibrosis transmembrane conductance regulator","score":0.906000018119812},{"id":"https://openalex.org/keywords/escherichia-coli-expression","display_name":"Escherichia coli expression","score":0.7770000100135803},{"id":"https://openalex.org/keywords/low-temperature-induction","display_name":"low temperature induction","score":0.8740000128746033},{"id":"https://openalex.org/keywords/urea-solubilization","display_name":"urea solubilization","score":0.9860000014305115},{"id":"https://openalex.org/keywords/protein-folding","display_name":"protein folding","score":0.6140000224113464},{"id":"https://openalex.org/keywords/one-step-purification","display_name":"one-step purification","score":0.7089999914169312}],"concepts":[{"id":"https://openalex.org/C538909803","wikidata":"https://www.wikidata.org/wiki/Q116907","display_name":"Glutathione","level":3,"score":0.7690639495849609},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.7327392101287842},{"id":"https://openalex.org/C2776907368","wikidata":"https://www.wikidata.org/wiki/Q307302","display_name":"Glutathione S-transferase","level":4,"score":0.7129371166229248},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6983699798583984},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6833590269088745},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5912171602249146},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.5707606077194214},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5334798097610474},{"id":"https://openalex.org/C2778476535","wikidata":"https://www.wikidata.org/wiki/Q390697","display_name":"Agarose","level":2,"score":0.46862155199050903},{"id":"https://openalex.org/C2776376580","wikidata":"https://www.wikidata.org/wiki/Q407355","display_name":"Transferase","level":3,"score":0.45316898822784424},{"id":"https://openalex.org/C71095615","wikidata":"https://www.wikidata.org/wiki/Q893051","display_name":"Tandem affinity purification","level":4,"score":0.45206862688064575},{"id":"https://openalex.org/C102362939","wikidata":"https://www.wikidata.org/wiki/Q417062","display_name":"Chaotropic agent","level":2,"score":0.4486147463321686},{"id":"https://openalex.org/C8985478","wikidata":"https://www.wikidata.org/wiki/Q903485","display_name":"Protein purification","level":2,"score":0.4117535948753357},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3228400945663452},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.23566105961799622},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.06548291444778442}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1002/bit.260390805","doi":"https://doi.org/10.1002/bit.260390805","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.260390805","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"pmid:18601017","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18601017","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and bioengineering","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W168199122","https://openalex.org/W643035040","https://openalex.org/W1806791563","https://openalex.org/W1975352932","https://openalex.org/W2012604733","https://openalex.org/W2022287320","https://openalex.org/W2023183303","https://openalex.org/W2039599894","https://openalex.org/W2049476833","https://openalex.org/W2068446924","https://openalex.org/W2072211198","https://openalex.org/W2093915148","https://openalex.org/W2156044856","https://openalex.org/W2269832643"],"related_works":["https://openalex.org/W4285298182","https://openalex.org/W2068881539","https://openalex.org/W2068446924","https://openalex.org/W1592167581","https://openalex.org/W2194767941","https://openalex.org/W2056707758","https://openalex.org/W2283475069","https://openalex.org/W3032619535","https://openalex.org/W2011163572","https://openalex.org/W2032539526"],"abstract_inverted_index":{"Prokaryotic":[0],"expression":[1],"of":[2,17,23,35,58,76,91,105,126,141,171,180],"polypeptides":[3,193],"as":[4,13,195],"fusion":[5,51,113,127,143,173],"proteins":[6,37,52,114,144],"with":[7,133,161,197],"glutathione-S-transferase":[8,54,93,142],"has":[9],"recently":[10],"been":[11],"reported":[12],"a":[14,116],"one-step":[15,83,139],"means":[16],"purifying":[18],"recombinant":[19,36,92,112,182],"protein.":[20,174],"The":[21],"usefulness":[22,179],"the":[24,32,59,103,178],"glutathione-S-transferase/glutathione-agarose":[25],"system,":[26],"however,":[27],"is":[28],"significantly":[29],"limited":[30],"by":[31,41,72],"frequent":[33],"synthesis":[34],"in":[38,69,155],"insoluble":[39,70,111],"form":[40,71,118],"Escherichia":[42],"coli.":[43,74],"We":[44],"have":[45],"found":[46],"that":[47],"for":[48,109],"5":[49],"separate":[50],"containing":[53],"and":[55,186],"different":[56],"domains":[57],"large":[60],"cystic":[61],"fibrosis":[62],"transmembrane":[63],"conductance":[64],"regulator,":[65],"all":[66],"were":[67],"packaged":[68],"E.":[73,146],"Insolubility":[75],"these":[77],"products":[78],"made":[79],"them":[80],"inaccessible":[81],"to":[82,94,115,120,137,191],"purification":[84,140,184],"utilizing":[85],"this":[86,181],"scheme":[87],"requires":[88],"proper":[89],"folding":[90],"allow":[95],"recognition":[96],"on":[97],"glutathione":[98],"affinity":[99,122],"agarose,":[100],"we":[101],"investigated":[102],"suitability":[104],"several":[106],"alternative":[107],"approaches":[108],"converting":[110],"soluble":[117],"amenable":[119],"glutathione-agarose":[121],"purification.":[123],"Low-temperature":[124],"induction":[125],"protein":[128,183],"synthesis,":[129],"but":[130,159],"not":[131,160],"incubation":[132],"anion-exchange":[134],"resins,":[135],"led":[136],"improved":[138],"from":[145],"coli":[147],"cell":[148],"lysate":[149],"using":[150],"mild,":[151],"nondenaturing":[152],"conditions.":[153],"Solubilization":[154],"8":[156],"mol/L":[157],"urea,":[158],"other":[162],"chaotropic":[163],"agents":[164],"or":[165],"detergents,":[166],"also":[167],"allowed":[168],"preparative":[169],"yields":[170],"affinity-purified":[172],"These":[175],"techniques":[176],"increase":[177],"scheme,":[185],"should":[187],"be":[188],"broadly":[189],"applicable":[190],"diverse":[192],"synthesized":[194],"fusions":[196],"glutathione-S-transferase.":[198]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-27T15:51:10.671819","created_date":"2016-06-24T00:00:00"}
