{"id":"https://openalex.org/W2086716570","doi":"https://doi.org/10.1021/ac401165s","title":"Isolation and in Vitro Culture of Rare Cancer Stem Cells from Patient-Derived Xenografts of Pancreatic Ductal Adenocarcinoma","display_name":"Isolation and in Vitro Culture of Rare Cancer Stem Cells from Patient-Derived Xenografts of Pancreatic Ductal Adenocarcinoma","publication_year":2013,"publication_date":"2013-07-01","ids":{"openalex":"https://openalex.org/W2086716570","doi":"https://doi.org/10.1021/ac401165s","mag":"2086716570","pmid":"https://pubmed.ncbi.nlm.nih.gov/23815678","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3754897"},"language":"en","primary_location":{"id":"doi:10.1021/ac401165s","doi":"https://doi.org/10.1021/ac401165s","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac401165s","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","datacite","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3754897","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5033923147","display_name":"Philip C. Gach","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Philip C. Gach","raw_affiliation_strings":["Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina, 27599, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina, 27599, United States","institution_ids":["https://openalex.org/I114027177"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021664491","display_name":"Peter J. Attayek","orcid":"https://orcid.org/0000-0001-7572-6381","observed_orcids":["https://orcid.org/0000-0001-7572-6381"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I137902535","display_name":"North Carolina State University","ror":"https://ror.org/04tj63d06","country_code":"US","type":"education","lineage":["https://openalex.org/I137902535"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peter J. Attayek","raw_affiliation_strings":["Department of Biomedical Engineering,\rUniversity of North Carolina, Chapel Hill, North Carolina 27599, United\rStates and North Carolina State University, Raleigh, North Carolina\r27695, United States","Department of Biomedical Engineering,#R#University of North Carolina, Chapel Hill, North Carolina 27599, United#R#States and North Carolina State University, Raleigh, North Carolina#R#27695, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering,\rUniversity of North Carolina, Chapel Hill, North Carolina 27599, United\rStates and North Carolina State University, Raleigh, North Carolina\r27695, United States","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I137902535"]},{"raw_affiliation_string":"Department of Biomedical Engineering,#R#University of North Carolina, Chapel Hill, North Carolina 27599, United#R#States and North Carolina State University, Raleigh, North Carolina#R#27695, United States","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I137902535"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5125330811","display_name":"Gabriela Herrera","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gabriela Herrera","raw_affiliation_strings":["Lineberger\rComprehensive Cancer\rCenter, University of North Carolina, Chapel Hill, North Carolina\r27599, United States","Lineberger#R#Comprehensive Cancer#R#Center, University of North Carolina, Chapel Hill, North Carolina#R#27599, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lineberger\rComprehensive Cancer\rCenter, University of North Carolina, Chapel Hill, North Carolina\r27599, United States","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]},{"raw_affiliation_string":"Lineberger#R#Comprehensive Cancer#R#Center, University of North Carolina, Chapel Hill, North Carolina#R#27599, United States","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065098287","display_name":"Jen Jen Yeh","orcid":"https://orcid.org/0000-0003-2542-6211","observed_orcids":["https://orcid.org/0000-0003-2542-6211"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jen Jen Yeh","raw_affiliation_strings":["Department\rof Surgery, Division\rof Surgical Oncology, University of North Carolina, Chapel Hill, North\rCarolina 27599, United States","Department of Pharmacology,\rUniversity of North Carolina, Chapel Hill, North Carolina 27599, United\rStates","Lineberger\rComprehensive Cancer\rCenter, University of North Carolina, Chapel Hill, North Carolina\r27599, United States","Lineberger#R#Comprehensive Cancer#R#Center, University of North Carolina, Chapel Hill, North Carolina#R#27599, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department\rof Surgery, Division\rof Surgical Oncology, University of North Carolina, Chapel Hill, North\rCarolina 27599, United States","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Department of Pharmacology,\rUniversity of North Carolina, Chapel Hill, North Carolina 27599, United\rStates","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Lineberger\rComprehensive Cancer\rCenter, University of North Carolina, Chapel Hill, North Carolina\r27599, United States","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]},{"raw_affiliation_string":"Lineberger#R#Comprehensive Cancer#R#Center, University of North Carolina, Chapel Hill, North Carolina#R#27599, United States","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5048509828","display_name":"Nancy L. Allbritton","orcid":"https://orcid.org/0000-0002-9242-768X","observed_orcids":["https://orcid.org/0000-0002-9242-768X"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I137902535","display_name":"North Carolina State University","ror":"https://ror.org/04tj63d06","country_code":"US","type":"education","lineage":["https://openalex.org/I137902535"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Nancy L. Allbritton","raw_affiliation_strings":["Department of Biomedical Engineering,\rUniversity of North Carolina, Chapel Hill, North Carolina 27599, United\rStates and North Carolina State University, Raleigh, North Carolina\r27695, United States","Department of Chemistry, University\rof North Carolina, Chapel Hill, North Carolina 27599, United States","Department of Pharmacology,\rUniversity of North Carolina, Chapel Hill, North Carolina 27599, United\rStates","Lineberger\rComprehensive Cancer\rCenter, University of North Carolina, Chapel Hill, North Carolina\r27599, United States","Department of Chemistry, University#R#of North Carolina, Chapel Hill, North Carolina 27599, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering,\rUniversity of North Carolina, Chapel Hill, North Carolina 27599, United\rStates and North Carolina State University, Raleigh, North Carolina\r27695, United States","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I137902535"]},{"raw_affiliation_string":"Department of Chemistry, University\rof North Carolina, Chapel Hill, North Carolina 27599, United States","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Department of Pharmacology,\rUniversity of North Carolina, Chapel Hill, North Carolina 27599, United\rStates","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Lineberger\rComprehensive Cancer\rCenter, University of North Carolina, Chapel Hill, North Carolina\r27599, United States","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]},{"raw_affiliation_string":"Department of Chemistry, University#R#of North Carolina, Chapel Hill, North Carolina 27599, United States","institution_ids":["https://openalex.org/I114027177"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5048509828"],"corresponding_institution_ids":["https://openalex.org/I114027177","https://openalex.org/I137902535","https://openalex.org/I4390039329"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.1741,"has_fulltext":true,"cited_by_count":10,"citation_normalized_percentile":{"value":0.78225429,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"85","issue":"15","first_page":"7271","last_page":"7278"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10336","display_name":"Cancer Cells and Metastasis","score":0.76419997215271,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10336","display_name":"Cancer Cells and Metastasis","score":0.76419997215271,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11255","display_name":"Microfluidic and Bio-sensing Technologies","score":0.06930000334978104,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10231","display_name":"Pancreatic and Hepatic Oncology Research","score":0.03680000081658363,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cancer-stem-cells","display_name":"cancer stem cells","score":0.9869999885559082},{"id":"https://openalex.org/keywords/pancreatic-ductal-adenocarcinoma","display_name":"pancreatic ductal adenocarcinoma","score":0.9570000171661377},{"id":"https://openalex.org/keywords/patient-derived-xenografts","display_name":"patient-derived xenografts","score":0.9769999980926514},{"id":"https://openalex.org/keywords/rare-cell-isolation","display_name":"rare cell isolation","score":0.8299999833106995},{"id":"https://openalex.org/keywords/fluorescence-screening","display_name":"fluorescence screening","score":0.6439999938011169},{"id":"https://openalex.org/keywords/tumor-spheroids","display_name":"tumor spheroids","score":0.753000020980835},{"id":"https://openalex.org/keywords/cd24","display_name":"CD24","score":0.9670000076293945},{"id":"https://openalex.org/keywords/cd44","display_name":"CD44","score":1.0},{"id":"https://openalex.org/keywords/epcam","display_name":"EpCAM","score":0.9700000286102295},{"id":"https://openalex.org/keywords/panc-1-cells","display_name":"PANC-1 cells","score":0.8920000195503235},{"id":"https://openalex.org/keywords/in-vitro-culture","display_name":"in vitro culture","score":0.5519999861717224},{"id":"https://openalex.org/keywords/cell-sorting","display_name":"cell sorting","score":0.4359999895095825}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8297669887542725},{"id":"https://openalex.org/C2992026798","wikidata":"https://www.wikidata.org/wiki/Q212961","display_name":"Pancreatic ductal adenocarcinoma","level":4,"score":0.7388730049133301},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.6506537199020386},{"id":"https://openalex.org/C2775941552","wikidata":"https://www.wikidata.org/wiki/Q25212305","display_name":"Isolation (microbiology)","level":2,"score":0.5640977621078491},{"id":"https://openalex.org/C2781182431","wikidata":"https://www.wikidata.org/wiki/Q356033","display_name":"Adenocarcinoma","level":3,"score":0.5623403191566467},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.5119599103927612},{"id":"https://openalex.org/C2780210213","wikidata":"https://www.wikidata.org/wiki/Q212961","display_name":"Pancreatic cancer","level":3,"score":0.5113603472709656},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.4991624355316162},{"id":"https://openalex.org/C55427017","wikidata":"https://www.wikidata.org/wiki/Q1638475","display_name":"Cancer stem cell","level":3,"score":0.4120021462440491},{"id":"https://openalex.org/C143998085","wikidata":"https://www.wikidata.org/wiki/Q162555","display_name":"Oncology","level":1,"score":0.3630084991455078},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.35702112317085266},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3354622423648834},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.11482506990432739},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.07017242908477783},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.05640977621078491},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.04350599646568298}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002471","descriptor_name":"Cell Transformation, Neoplastic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010190","descriptor_name":"Pancreatic Neoplasms","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D014411","descriptor_name":"Neoplastic Stem Cells","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D021441","descriptor_name":"Carcinoma, Pancreatic Ductal","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1021/ac401165s","doi":"https://doi.org/10.1021/ac401165s","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac401165s","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"pmid:23815678","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23815678","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:3754897","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3754897","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Anal Chem","raw_type":"Text"},{"id":"pmh:cdr.lib.unc.edu:2n49t781t","doi":null,"is_oa":true,"landing_page_url":"https://doi.org/10.17615/qpyj-9493","pdf_url":"https://doi.org/10.17615/qpyj-9493","source":{"id":"https://openalex.org/S4306401075","display_name":"Carolina Digital Repository (University of North Carolina at Chapel Hill)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114027177","host_organization_name":"University of North Carolina at Chapel Hill","host_organization_lineage":["https://openalex.org/I114027177"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Analytical Chemistry, 85(15)","raw_type":"Article"},{"id":"doi:10.17615/qpyj-9493","doi":"https://doi.org/10.17615/qpyj-9493","is_oa":true,"landing_page_url":"https://doi.org/10.17615/qpyj-9493","pdf_url":null,"source":{"id":"https://openalex.org/S7407051488","display_name":"UNC Libraries","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:3754897","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3754897","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Anal Chem","raw_type":"Text"},"sustainable_development_goals":[{"score":0.766700029373169,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1650860556","display_name":"Multiplexed Measurement of Kinase Activity in Single Cancer Cells","funder_award_id":"R01CA139599","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G3752788551","display_name":"Arrays for Cloning Growth Suppressed Cells","funder_award_id":"R01EB012549","funder_id":"https://openalex.org/F4320337363","funder_display_name":"National Institute of Biomedical Imaging and Bioengineering"},{"id":"https://openalex.org/G5182618377","display_name":"Targeting Ras-Ral GEF-Ral Effector Signaling for Pancreatic Cancer Treatment","funder_award_id":"R01CA140424","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G9000913976","display_name":"Cancer Center Support Grant","funder_award_id":"P30CA016086","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320322725","display_name":"China Scholarship Council","ror":"https://ror.org/04atp4p48"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332600","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337363","display_name":"National Institute of Biomedical Imaging and Bioengineering","ror":"https://ror.org/00372qc85"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2086716570.pdf","grobid_xml":"https://content.openalex.org/works/W2086716570.grobid-xml"},"referenced_works_count":34,"referenced_works":["https://openalex.org/W600147695","https://openalex.org/W1495945591","https://openalex.org/W1558034062","https://openalex.org/W1972783166","https://openalex.org/W2001544973","https://openalex.org/W2007897263","https://openalex.org/W2009886857","https://openalex.org/W2019264784","https://openalex.org/W2021883029","https://openalex.org/W2023427658","https://openalex.org/W2030687926","https://openalex.org/W2034219288","https://openalex.org/W2039634179","https://openalex.org/W2042267487","https://openalex.org/W2050300062","https://openalex.org/W2050547995","https://openalex.org/W2057306577","https://openalex.org/W2059024717","https://openalex.org/W2060111924","https://openalex.org/W2069610810","https://openalex.org/W2083970667","https://openalex.org/W2093783043","https://openalex.org/W2095431158","https://openalex.org/W2102085111","https://openalex.org/W2111976640","https://openalex.org/W2133003941","https://openalex.org/W2139532459","https://openalex.org/W2141650341","https://openalex.org/W2153382986","https://openalex.org/W2162828533","https://openalex.org/W2165948908","https://openalex.org/W2505399031","https://openalex.org/W3217380390","https://openalex.org/W4205433743"],"related_works":["https://openalex.org/W2936446508","https://openalex.org/W2036773381","https://openalex.org/W2885413121","https://openalex.org/W7098717915","https://openalex.org/W4235665856","https://openalex.org/W2917505199","https://openalex.org/W1482011127","https://openalex.org/W7097668497","https://openalex.org/W4281753029","https://openalex.org/W2042267487"],"abstract_inverted_index":{"Described":[0],"is":[1],"the":[2,127,195,211,232,274],"construction":[3],"of":[4,8,40,64,81,122,139,164,194,213,220,231,238,250,270,276],"a":[5,30,57,96,146,153,192],"large":[6,58],"array":[7,39,63,77,128],"releasable":[9,68],"microstructures":[10],"(micropallets)":[11],"along":[12],"with":[13,95,110,116,158,167],"screening":[14,78,131],"and":[15,49,150,184,205,216,226,236,253,273],"isolation":[16,225],"protocols":[17],"for":[18,79,187,210],"sorting":[19,170,179],"rare,":[20],"approximately":[21,65],"1":[22],"in":[23,85],"10,000,":[24],"cancer":[25],"stem":[26],"cells":[27,84,108,119,138,200,235,242,260],"(CSCs)":[28],"from":[29,201,261],"heterogeneous":[31],"cell":[32,169,189,203,224,271],"population.":[33],"A":[34,161],"10.1":[35],"\u00d7":[36,43,45],"7.1":[37],"cm":[38],"micropallets":[41],"(50":[42],"50":[44],"75":[46],"\u03bcm":[47,51],"structures":[48],"25":[50],"micropallet":[52,174],"gap)":[53],"was":[54,103],"fabricated":[55],"on":[56,126,152],"glass":[59],"substrate,":[60],"providing":[61],"an":[62,91],"1.3":[66],"million":[67],"microstructures.":[69],"Image":[70],"analysis":[71],"algorithms":[72],"were":[73,141,208],"developed":[74],"to":[75,124,132,257],"permit":[76],"identification":[80],"fluorescently":[82],"labeled":[83],"less":[86],"than":[87],"15":[88],"min":[89],"using":[90],"epifluorescent":[92],"wide-field":[93],"microscope":[94],"computer":[97],"controlled":[98],"translational":[99],"stage.":[100],"Device":[101],"operation":[102],"tested":[104],"by":[105,130,145],"culturing":[106],"HeLa":[107,118],"transfected":[109],"green":[111],"fluorescent":[112],"protein":[113],"(GFP)":[114],"admixed":[115],"wild-type":[117],"at":[120],"ratios":[121],"1:10(4)":[123],"1:10(6)":[125],"followed":[129],"identify":[133],"flourescent":[134],"cells.":[135],"Micropallets":[136],"containing":[137],"interest":[140],"then":[142],"selectively":[143],"released":[144],"focused":[147],"laser":[148],"pulse":[149],"collected":[151],"numbered":[154],"poly(dimethylsiloxane)":[155],"(PDMS)":[156],"substrate":[157],"high":[159,247],"viability.":[160],"direct":[162],"comparison":[163],"this":[165],"technology":[166],"fluorescence-activated":[168],"(FACS)":[171],"demonstrated":[172],"that":[173],"arrays":[175],"offered":[176],"enhanced":[177],"post":[178],"purity":[180],"(100%),":[181,183],"yield":[182],"viability":[185],"(94-100%)":[186],"rare":[188,259],"isolation.":[190],"As":[191],"demonstration":[193],"technology's":[196],"value,":[197],"pancreatic":[198,221],"tumor":[199,244],"Panc-1":[202,234],"lines":[204],"patient-derived":[206],"xenografts":[207],"screened":[209],"presence":[212],"CD24,":[214,251],"CD44,":[215,252],"CD326:":[217],"surface":[218],"markers":[219],"CSCs.":[222],"Following":[223],"culture,":[227],"63":[228],"\u00b1":[229],"23%":[230],"isolated":[233],"35%":[237],"sorted":[239],"human":[240],"xenograft":[241],"formed":[243],"spheroids":[245],"retaining":[246],"expression":[248],"levels":[249],"CD326.":[254],"The":[255],"ability":[256],"isolate":[258],"relatively":[262],"small":[263],"sample":[264],"sizes":[265],"will":[266],"facilitate":[267],"our":[268],"understanding":[269],"biology":[272],"development":[275],"new":[277],"therapeutic":[278],"strategies.":[279]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}