{"id":"https://openalex.org/W2085645098","doi":"https://doi.org/10.1002/jgm.726","title":"Generation of a packaging cell line for prolonged large\u2010scale production of high\u2010titer HIV\u20101\u2010based lentiviral vector","display_name":"Generation of a packaging cell line for prolonged large\u2010scale production of high\u2010titer HIV\u20101\u2010based lentiviral vector","publication_year":2005,"publication_date":"2005-02-03","ids":{"openalex":"https://openalex.org/W2085645098","doi":"https://doi.org/10.1002/jgm.726","mag":"2085645098","pmid":"https://pubmed.ncbi.nlm.nih.gov/15693055"},"language":"en","primary_location":{"id":"doi:10.1002/jgm.726","doi":"https://doi.org/10.1002/jgm.726","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jgm.726","pdf_url":null,"source":{"id":"https://openalex.org/S91057606","display_name":"The Journal of Gene Medicine","issn_l":"1099-498X","issn":["1099-498X","1521-2254"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Gene Medicine","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026320415","display_name":"Yajin Ni","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yajin Ni","raw_affiliation_strings":["VIRxSYS Corporation, Gaithersburg, Maryland 20877 [correction] USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VIRxSYS Corporation, Gaithersburg, Maryland 20877 [correction] USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103116987","display_name":"Susan Y. Sun","orcid":"https://orcid.org/0000-0001-9526-4639","observed_orcids":["https://orcid.org/0000-0001-9526-4639"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Susan Sun","raw_affiliation_strings":["VIRXSYS Corporation, Gaithersburg, MD 20878, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VIRXSYS Corporation, Gaithersburg, MD 20878, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075528718","display_name":"Ibe Oparaocha","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ibe Oparaocha","raw_affiliation_strings":["VIRXSYS Corporation, Gaithersburg, MD 20878, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VIRXSYS Corporation, Gaithersburg, MD 20878, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015488396","display_name":"Laurent M. P. F. Humeau","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Laurent Humeau","raw_affiliation_strings":["VIRXSYS Corporation, Gaithersburg, MD 20878, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VIRXSYS Corporation, Gaithersburg, MD 20878, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Brian Davis","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Brian Davis","raw_affiliation_strings":["VIRXSYS Corporation, Gaithersburg, MD 20878, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VIRXSYS Corporation, Gaithersburg, MD 20878, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074763899","display_name":"Reuben Cohen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Reuben Cohen","raw_affiliation_strings":["VIRXSYS Corporation, Gaithersburg, MD 20878, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VIRXSYS Corporation, Gaithersburg, MD 20878, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056610348","display_name":"Gwendolyn K. Binder","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Gwendolyn Binder","raw_affiliation_strings":["VIRXSYS Corporation, Gaithersburg, MD 20878, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VIRXSYS Corporation, Gaithersburg, MD 20878, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110101091","display_name":"Yung\u2010Nien Chang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yung\u2010Nien Chang","raw_affiliation_strings":["VIRXSYS Corporation, Gaithersburg, MD 20878, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VIRXSYS Corporation, Gaithersburg, MD 20878, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087101621","display_name":"Vladimir Slepushkin","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Vladimir Slepushkin","raw_affiliation_strings":["VIRXSYS Corporation, Gaithersburg, MD 20878, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"VIRXSYS Corporation, Gaithersburg, MD 20878, USA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5003913525","display_name":"Boro Dropuli\u0107","orcid":"https://orcid.org/0000-0002-3861-7677","observed_orcids":["https://orcid.org/0000-0002-3861-7677"]},"institutions":[{"id":"https://openalex.org/I4210126252","display_name":"Lentigen Technology (United States)","ror":"https://ror.org/0333j1f77","country_code":"US","type":"company","lineage":["https://openalex.org/I4210126252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Boro Dropulic","raw_affiliation_strings":["Lentigen Corporation, 6030 Daybreak Circle, Suite A150-333, Clarksville, MD 21029, USA","Lentigen Corporation, 6030 Daybreak Circle, Suite A150\u2010333, Clarksville, MD 21029, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lentigen Corporation, 6030 Daybreak Circle, Suite A150-333, Clarksville, MD 21029, USA","institution_ids":["https://openalex.org/I4210126252"]},{"raw_affiliation_string":"Lentigen Corporation, 6030 Daybreak Circle, Suite A150\u2010333, Clarksville, MD 21029, USA","institution_ids":["https://openalex.org/I4210126252"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5087101621"],"corresponding_institution_ids":[],"apc_list":{"value":4480,"currency":"USD","value_usd":4480},"apc_paid":null,"fwci":2.5196,"has_fulltext":false,"cited_by_count":77,"citation_normalized_percentile":{"value":0.88920561,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"7","issue":"6","first_page":"818","last_page":"834"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lentiviral-vector-production","display_name":"lentiviral vector production","score":0.8109999895095825},{"id":"https://openalex.org/keywords/packaging-cell-lines","display_name":"packaging cell lines","score":0.9300000071525574},{"id":"https://openalex.org/keywords/tetracycline-transactivator","display_name":"tetracycline transactivator","score":0.9229999780654907},{"id":"https://openalex.org/keywords/gene-silencing","display_name":"gene silencing","score":0.8349999785423279},{"id":"https://openalex.org/keywords/cytotoxicity","display_name":"cytotoxicity","score":0.5600000023841858},{"id":"https://openalex.org/keywords/cd34-cells","display_name":"CD34+ cells","score":0.9269999861717224},{"id":"https://openalex.org/keywords/vector-titer","display_name":"vector titer","score":0.4819999933242798}],"concepts":[{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.699576199054718},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.6668712496757507},{"id":"https://openalex.org/C32470452","wikidata":"https://www.wikidata.org/wiki/Q2298489","display_name":"Viral vector","level":4,"score":0.6537054777145386},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.612575888633728},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5818783640861511},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.572060763835907},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.547515332698822},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.526920735836029},{"id":"https://openalex.org/C2779690655","wikidata":"https://www.wikidata.org/wiki/Q374232","display_name":"Lentivirus","level":4,"score":0.5043121576309204},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.476629376411438},{"id":"https://openalex.org/C32611913","wikidata":"https://www.wikidata.org/wiki/Q2166189","display_name":"Titer","level":3,"score":0.47400128841400146},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.47123846411705017},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.454496294260025},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3781755864620209},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.2259979546070099},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.18663400411605835},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.1172204315662384},{"id":"https://openalex.org/C2780727368","wikidata":"https://www.wikidata.org/wiki/Q1928978","display_name":"Viral disease","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002999","descriptor_name":"Clone Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002999","descriptor_name":"Clone Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002999","descriptor_name":"Clone Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003062","descriptor_name":"Codon","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003062","descriptor_name":"Codon","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003062","descriptor_name":"Codon","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008562","descriptor_name":"Membrane Glycoproteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008562","descriptor_name":"Membrane Glycoproteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008562","descriptor_name":"Membrane Glycoproteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008957","descriptor_name":"Models, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008957","descriptor_name":"Models, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008957","descriptor_name":"Models, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013752","descriptor_name":"Tetracycline","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013752","descriptor_name":"Tetracycline","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013752","descriptor_name":"Tetracycline","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014161","descriptor_name":"Transduction, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014161","descriptor_name":"Transduction, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014161","descriptor_name":"Transduction, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014759","descriptor_name":"Viral Envelope Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014759","descriptor_name":"Viral Envelope Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014759","descriptor_name":"Viral Envelope Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014771","descriptor_name":"Virion","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014771","descriptor_name":"Virion","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014771","descriptor_name":"Virion","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014779","descriptor_name":"Virus Replication","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014779","descriptor_name":"Virus Replication","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014779","descriptor_name":"Virus Replication","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015497","descriptor_name":"HIV-1","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015497","descriptor_name":"HIV-1","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015497","descriptor_name":"HIV-1","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015685","descriptor_name":"Fusion Proteins, gag-pol","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015685","descriptor_name":"Fusion Proteins, gag-pol","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015685","descriptor_name":"Fusion Proteins, gag-pol","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015685","descriptor_name":"Fusion Proteins, gag-pol","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015685","descriptor_name":"Fusion Proteins, gag-pol","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015685","descriptor_name":"Fusion Proteins, gag-pol","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015694","descriptor_name":"Gene Products, rev","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015694","descriptor_name":"Gene Products, rev","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015694","descriptor_name":"Gene Products, rev","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015694","descriptor_name":"Gene Products, rev","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015694","descriptor_name":"Gene Products, rev","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015694","descriptor_name":"Gene Products, rev","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015696","descriptor_name":"Gene Products, tat","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015696","descriptor_name":"Gene Products, tat","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015696","descriptor_name":"Gene Products, tat","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015696","descriptor_name":"Gene Products, tat","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015696","descriptor_name":"Gene Products, tat","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015696","descriptor_name":"Gene Products, tat","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D016086","descriptor_name":"Lentivirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016086","descriptor_name":"Lentivirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016086","descriptor_name":"Lentivirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D054321","descriptor_name":"rev Gene Products, Human Immunodeficiency Virus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D054321","descriptor_name":"rev Gene Products, Human Immunodeficiency Virus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D054321","descriptor_name":"rev Gene Products, Human Immunodeficiency Virus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D054322","descriptor_name":"tat Gene Products, Human Immunodeficiency Virus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D054322","descriptor_name":"tat Gene Products, Human Immunodeficiency Virus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D054322","descriptor_name":"tat Gene Products, Human Immunodeficiency Virus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/jgm.726","doi":"https://doi.org/10.1002/jgm.726","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jgm.726","pdf_url":null,"source":{"id":"https://openalex.org/S91057606","display_name":"The Journal of Gene Medicine","issn_l":"1099-498X","issn":["1099-498X","1521-2254"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Gene Medicine","raw_type":"journal-article"},{"id":"pmid:15693055","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15693055","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The journal of gene medicine","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/3","score":0.6499999761581421,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":43,"referenced_works":["https://openalex.org/W1522200518","https://openalex.org/W1835940621","https://openalex.org/W1976365653","https://openalex.org/W1976694988","https://openalex.org/W1997571548","https://openalex.org/W2001056113","https://openalex.org/W2003113791","https://openalex.org/W2004048959","https://openalex.org/W2021534641","https://openalex.org/W2026901855","https://openalex.org/W2027122999","https://openalex.org/W2035444138","https://openalex.org/W2038069104","https://openalex.org/W2038780043","https://openalex.org/W2048464014","https://openalex.org/W2052754749","https://openalex.org/W2054261154","https://openalex.org/W2057889496","https://openalex.org/W2059003079","https://openalex.org/W2060484288","https://openalex.org/W2065189089","https://openalex.org/W2066699285","https://openalex.org/W2072291835","https://openalex.org/W2075623261","https://openalex.org/W2087473487","https://openalex.org/W2089710389","https://openalex.org/W2093632845","https://openalex.org/W2097309207","https://openalex.org/W2102535882","https://openalex.org/W2107266668","https://openalex.org/W2114156985","https://openalex.org/W2122504547","https://openalex.org/W2127345803","https://openalex.org/W2136677510","https://openalex.org/W2144144425","https://openalex.org/W2146702776","https://openalex.org/W2147167308","https://openalex.org/W2157894968","https://openalex.org/W2165116671","https://openalex.org/W2165729635","https://openalex.org/W2320256363","https://openalex.org/W2529969481","https://openalex.org/W4242794150"],"related_works":["https://openalex.org/W2347300262","https://openalex.org/W2374090291","https://openalex.org/W2350467418","https://openalex.org/W4230010807","https://openalex.org/W2028607742","https://openalex.org/W181974884","https://openalex.org/W1602083480","https://openalex.org/W2065266863","https://openalex.org/W2394205039","https://openalex.org/W2018609975"],"abstract_inverted_index":{"BACKGROUND:":[0],"A":[1],"stable":[2,144],"packaging":[3,24,33,50,90,101,269],"cell":[4,25,180,270],"line":[5],"facilitates":[6],"large-scale":[7,138],"lentivirus":[8,184],"vector":[9,118,145,214,221,261],"manufacture.":[10],"However,":[11],"it":[12],"has":[13],"been":[14],"difficult":[15],"to":[16,30,66,78,103],"produce":[17],"clinical-scale":[18],"HIV-1-based":[19,259],"lentiviral":[20,260],"vectors":[21],"using":[22,267],"a":[23,59,95,148,268],"line,":[26],"in":[27,205,223,226],"part":[28],"due":[29],"toxicity":[31],"of":[32,49,84,89,98,115],"genes,":[34],"and":[35,86,117,131,164,173,242],"gene":[36,62,199,218],"silencing":[37,200],"that":[38],"occurs":[39,201],"during":[40],"the":[41,99,105,123,155,193,208,256,264],"long":[42],"culture":[43,106,245],"period":[44,149],"necessary":[45],"for":[46,108,137,255],"sequential":[47],"addition":[48],"constructs.":[51],"METHODS:":[52],"To":[53],"avoid":[54],"these":[55],"problems,":[56],"we":[57],"developed":[58],"three-level":[60,124],"cascade":[61],"regulation":[63,125],"system":[64],"designed":[65],"remove":[67],"tetracycline":[68],"transactivator":[69],"(tTA)":[70],"from":[71,81,178],"cytomegalovirus":[72],"immediate":[73],"early":[74],"promoter":[75],"(CMV)-controlled":[76],"expression":[77,83,88,114],"reduce":[79],"cytotoxicity":[80,128],"constitutive":[82],"tTA":[85],"leaky":[87,113],"genes.":[91],"We":[92],"also":[93],"performed":[94],"one-step":[96,209],"integration":[97,211],"three":[100],"plasmids":[102],"shorten":[104],"time":[107,258],"clonal":[109],"selection.":[110],"RESULTS:":[111],"Although":[112],"p24":[116,165,177],"production":[119,146,215,262],"still":[120],"occurred":[121],"despite":[122],"system,":[126],"little":[127],"was":[129,186,247],"observed":[130],"producer":[132],"cells":[133,141,194,236],"could":[134],"be":[135],"expanded":[136],"production.":[139],"Producer":[140],"yielded":[142],"remarkably":[143],"over":[147],"greater":[150],"than":[151],"11":[152],"days":[153],"with":[154],"highest":[156],"titer":[157],"3.5":[158],"x":[159,170],"10(7)":[160],"transducing":[161],"units":[162],"(TU)/ml":[163],"300":[166],"ng/ml,":[167],"yielding":[168],"2.2":[169],"10(11)":[171],"TU":[172,231],"1.8":[174],"milligram":[175],"(mg)":[176],"one":[179],"factory.":[181],"No":[182],"replication-competent":[183],"(RCL)":[185],"detected.":[187],"Long-term":[188],"analysis":[189],"demonstrated":[190],"that,":[191],"although":[192],"are":[195],"genetically":[196],"stable,":[197],"partial":[198],"after":[202],"2-3":[203],"months":[204],"culture;":[206],"however,":[207],"construct":[210],"allowed":[212],"prolonged":[213],"before":[216],"significant":[217],"silencing.":[219],"Concentrated":[220],"resulted":[222],"90%":[224],"transduction":[225],"CD4+":[227],"lymphocytes":[228],"at":[229,239,249],"20":[230],"per":[232],"cell.":[233],"CD34+":[234],"progenitor":[235],"were":[237],"transduced":[238,248],"41-46%":[240],"efficiency,":[241],"long-term":[243],"initiating":[244],"(LTC-IC)":[246],"45-51%.":[250],"CONCLUSIONS:":[251],"These":[252],"results":[253],"demonstrate":[254],"first":[257],"on":[263],"large":[265],"scale":[266],"line.":[271]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":6}],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2025-10-10T00:00:00"}